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hybridization buffer  (Nacalai)

 
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    Nacalai hybridization buffer
    Hybridization Buffer, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+buffer/buffer+hybridization/pm41638583-92-5-12
    Average 86 stars, based on 1 article reviews
    hybridization buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Hybridization:

    Article Title: Multiplex in situ hybridization chain reaction reveals the spatial organization of six pituitary hormone mRNAs in the Japanese eel.
    Article Snippet: The anterior pituitary gland regulates growth, development, metabolism, reproduction, and stress responses.. In this study, we established a two-round multiplex in situ hybridization chain reaction (isHCR) procedure combined with a fluorescence-quenching device to visualize the spatial distribution of six pituitary hormone mRNAs (gh, lhb, fshb, tshb, prl, and pomc) in cultured immature Japanese eels.. In pituitary sections, prl and tshb were localized to the rostral pars distalis (RPD), whereas lhb, fshb, and gh were distributed in the proximal pars distalis.

    Article Title: Fgf3 and Fgf10a regulate neuronal fasciculation through Schwann cell proliferation and infiltration in zebrafish posterior lateral line
    Article Snippet: Digoxigenin (DIG)-labelled RNA probes were synthesized from 200 ng of amplicon using DIG labelling kit (Roche) following the manufacturer’s protocol. .. The probes were denatured with 10 μl proteinase K (10 mg/ml) at 55°C for 30 minutes and precipitated with 100% ethanol, followed by washing with 75% ethanol and dissolved in hybridization buffer (60% formaldehyde, 5X SSC, 20% Tween 20 (Nacalai Tesque, Japan) and 50 mg/ml heparin (Sigma, USA). the quality and concentration of the probe were assessed by loading 0.5 μl RNA product onto a 1.5% agarose gel. ..

    Concentration Assay:

    Article Title: Fgf3 and Fgf10a regulate neuronal fasciculation through Schwann cell proliferation and infiltration in zebrafish posterior lateral line
    Article Snippet: Digoxigenin (DIG)-labelled RNA probes were synthesized from 200 ng of amplicon using DIG labelling kit (Roche) following the manufacturer’s protocol. .. The probes were denatured with 10 μl proteinase K (10 mg/ml) at 55°C for 30 minutes and precipitated with 100% ethanol, followed by washing with 75% ethanol and dissolved in hybridization buffer (60% formaldehyde, 5X SSC, 20% Tween 20 (Nacalai Tesque, Japan) and 50 mg/ml heparin (Sigma, USA). the quality and concentration of the probe were assessed by loading 0.5 μl RNA product onto a 1.5% agarose gel. ..

    Agarose Gel Electrophoresis:

    Article Title: Fgf3 and Fgf10a regulate neuronal fasciculation through Schwann cell proliferation and infiltration in zebrafish posterior lateral line
    Article Snippet: Digoxigenin (DIG)-labelled RNA probes were synthesized from 200 ng of amplicon using DIG labelling kit (Roche) following the manufacturer’s protocol. .. The probes were denatured with 10 μl proteinase K (10 mg/ml) at 55°C for 30 minutes and precipitated with 100% ethanol, followed by washing with 75% ethanol and dissolved in hybridization buffer (60% formaldehyde, 5X SSC, 20% Tween 20 (Nacalai Tesque, Japan) and 50 mg/ml heparin (Sigma, USA). the quality and concentration of the probe were assessed by loading 0.5 μl RNA product onto a 1.5% agarose gel. ..



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    (A, B) The C5-hFc-siSOD1 conjugate was dosed in WT mice as a single IV or SC bolus at 1.5 mg/kg (A) or 0.14 to 9 mg/kg siRNA equivalent dose (B). Target knockdown was measured 14 days after administration, using RT-qPCR on total RNA extracts from indicated regions of the CNS. The results are expressed as a ratio to mRNA levels measured in PBS-treated animals after normalization to RpL13 and RpL30 reference genes. (A) Box plots: extends from the 25th to 75th percentiles with center line plotted at the median. Whiskers indicate the smallest and the largest experimental values (n = 8-16 animals per group from 3 independent experiments). (B) Dose-response curves in each CNS region, where experimental results are expressed as means ± SEM, and estimated ED 50 values. (C) Mice were administered with either multi-IV (Q2Dx3) at 1.5 mg/kg or single SC at 4.5 mg/kg siRNA equivalent dose of the C5-hFc-siSOD1 conjugate. Shown are in situ <t>hybridization</t> micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections), and corresponding Sod1 mRNA (lower panels, RNAScope®), 14 days after the last IV or single SC (representative animal from n=4 mice per group). (D) Magnifications and neuron-specific co-staining, using a Map2 mRNA-specific probe, in indicated regions of the brain.
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    Image Search Results


    (A, B) The C5-hFc-siSOD1 conjugate was dosed in WT mice as a single IV or SC bolus at 1.5 mg/kg (A) or 0.14 to 9 mg/kg siRNA equivalent dose (B). Target knockdown was measured 14 days after administration, using RT-qPCR on total RNA extracts from indicated regions of the CNS. The results are expressed as a ratio to mRNA levels measured in PBS-treated animals after normalization to RpL13 and RpL30 reference genes. (A) Box plots: extends from the 25th to 75th percentiles with center line plotted at the median. Whiskers indicate the smallest and the largest experimental values (n = 8-16 animals per group from 3 independent experiments). (B) Dose-response curves in each CNS region, where experimental results are expressed as means ± SEM, and estimated ED 50 values. (C) Mice were administered with either multi-IV (Q2Dx3) at 1.5 mg/kg or single SC at 4.5 mg/kg siRNA equivalent dose of the C5-hFc-siSOD1 conjugate. Shown are in situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections), and corresponding Sod1 mRNA (lower panels, RNAScope®), 14 days after the last IV or single SC (representative animal from n=4 mice per group). (D) Magnifications and neuron-specific co-staining, using a Map2 mRNA-specific probe, in indicated regions of the brain.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: (A, B) The C5-hFc-siSOD1 conjugate was dosed in WT mice as a single IV or SC bolus at 1.5 mg/kg (A) or 0.14 to 9 mg/kg siRNA equivalent dose (B). Target knockdown was measured 14 days after administration, using RT-qPCR on total RNA extracts from indicated regions of the CNS. The results are expressed as a ratio to mRNA levels measured in PBS-treated animals after normalization to RpL13 and RpL30 reference genes. (A) Box plots: extends from the 25th to 75th percentiles with center line plotted at the median. Whiskers indicate the smallest and the largest experimental values (n = 8-16 animals per group from 3 independent experiments). (B) Dose-response curves in each CNS region, where experimental results are expressed as means ± SEM, and estimated ED 50 values. (C) Mice were administered with either multi-IV (Q2Dx3) at 1.5 mg/kg or single SC at 4.5 mg/kg siRNA equivalent dose of the C5-hFc-siSOD1 conjugate. Shown are in situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections), and corresponding Sod1 mRNA (lower panels, RNAScope®), 14 days after the last IV or single SC (representative animal from n=4 mice per group). (D) Magnifications and neuron-specific co-staining, using a Map2 mRNA-specific probe, in indicated regions of the brain.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: Knockdown, Quantitative RT-PCR, In Situ Hybridization, RNAscope, Staining

    (A-B) In situ hybridization micrographs showing siSOD1 AS distribution (miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections) (A), 14 days after multi-IV bolus (Q2D x3) of the non-binding C5neg- or the TfR1-binding B8V32-hFc-siSOD1 conjugates, at 1.5 mg/kg siRNA. (B) In situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) and corresponding Sod1 mRNA (lower panels, RNAScope®) in retina layers of the same animals (representative animal from n=4 mice per group). Retina layers: GCL, Ganglion Cell Layer; IPL, Inner Plexiform Layer; INL, Inner Nuclear Layer; OPL, Outer Plexiform Layer; ONL, Outer Nuclear Layer; IS, Inner Segments; OS, Outer Segments; RPE, Retinal Pigment Epithelium; Chor, Choroid.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: (A-B) In situ hybridization micrographs showing siSOD1 AS distribution (miRNAScope®) throughout the brain (sagittal sections) and spinal cord (cross-sections) (A), 14 days after multi-IV bolus (Q2D x3) of the non-binding C5neg- or the TfR1-binding B8V32-hFc-siSOD1 conjugates, at 1.5 mg/kg siRNA. (B) In situ hybridization micrographs showing siSOD1 AS distribution (top panels, miRNAScope®) and corresponding Sod1 mRNA (lower panels, RNAScope®) in retina layers of the same animals (representative animal from n=4 mice per group). Retina layers: GCL, Ganglion Cell Layer; IPL, Inner Plexiform Layer; INL, Inner Nuclear Layer; OPL, Outer Plexiform Layer; ONL, Outer Nuclear Layer; IS, Inner Segments; OS, Outer Segments; RPE, Retinal Pigment Epithelium; Chor, Choroid.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: In Situ Hybridization, Binding Assay, RNAscope

    Cynomolgus monkeys were dosed by 30-min IV infusion three times at 7 days intervals with the B8V32-hFc-siHPRT conjugate, at the same dose previously evaluated in hTfR-KI mice of 1.5 mg/kg siRNA equivalent dose. Plasma samples were collected at indicated timepoints for PK analysis (A). Tissues were recovered at 14 days after last injection to assess target knockdown using RT-qPCR (B) and in situ hybridization in the brain using RNAScope® on one coronal section through the putamen (top panels: approximately -1 to -3 mm from the anterior commissure, based on Calabrese et al. 2015) and one through the hippocampus (lower panels: approximately -11 to -13 mm from the anterior commissure; star indicates an artefact) (C). (D) Neuroinflammation was assessed on brain tissue samples by measurement of mRNA levels of IbA1, GFAP and C3 as markers of microglial reactivity, astrocyte reactivity and complement activation, respectively.

    Journal: bioRxiv

    Article Title: Harnessing TfR1 for Cross-Species Systemic Delivery of siRNAs to Deep Brain Regions Using Single-Domain Antibodies

    doi: 10.64898/2026.05.20.726486

    Figure Lengend Snippet: Cynomolgus monkeys were dosed by 30-min IV infusion three times at 7 days intervals with the B8V32-hFc-siHPRT conjugate, at the same dose previously evaluated in hTfR-KI mice of 1.5 mg/kg siRNA equivalent dose. Plasma samples were collected at indicated timepoints for PK analysis (A). Tissues were recovered at 14 days after last injection to assess target knockdown using RT-qPCR (B) and in situ hybridization in the brain using RNAScope® on one coronal section through the putamen (top panels: approximately -1 to -3 mm from the anterior commissure, based on Calabrese et al. 2015) and one through the hippocampus (lower panels: approximately -11 to -13 mm from the anterior commissure; star indicates an artefact) (C). (D) Neuroinflammation was assessed on brain tissue samples by measurement of mRNA levels of IbA1, GFAP and C3 as markers of microglial reactivity, astrocyte reactivity and complement activation, respectively.

    Article Snippet: For knock-down analysis on tissue sections using in situ hybridization (RNAScope, ACD), left cerebral hemisphere half-slices were cut into one ventral and one dorsal parts and immediately immersed in 10% neutral buffer formalin (NBF) fixative solution for approximately 48h at RT.

    Techniques: Clinical Proteomics, Injection, Knockdown, Quantitative RT-PCR, In Situ Hybridization, RNAscope, Activation Assay