luna script rt super mix kit (New England Biolabs)

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New England Biolabs luna script rt super mix kit
Luna Script Rt Super Mix Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/go+script+reverse+transcriptase+kits/LunaScript+RT+SuperMix+Kit/pm39864539-55-26-32
Average 99 stars, based on 1875 article reviews

luna script rt super mix kit - by Bioz Stars, 2026-10

99/100 stars

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Synthesized:

Article Title: Phosphoproteomic Insights into TRPV4–AMPK Signaling Axis and Barrier Regulation in the Choroid Plexus Epithelium
Article Snippet: RNA concentration was determined using a Nanodrop 2000 spectrophotometer (ThermoFisher) before cDNA synthesis. .. cDNA was synthesized from total RNA using LunaScript RT SuperMix Kit (NEB, Cat No: E3010L) and subsequently used for PCR analyses. ..

Article Title: Dose-response-defined physiological framework enables comparative analysis of transcriptional response dynamics across multiple herbicides in Arabidopsis thaliana
Article Snippet: .. First-strand cDNA was synthesized from DNase-treated RNA using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions. .. Custom DNA primers were synthesized by Sigma-Aldrich (St. Louis, MO, USA) ( ) and used for amplification with SYBR Green Universal Master Mix on an Applied Biosystems real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Dapagliflozin Prevents Heart Failure With Preserved Ejection Fraction by Inhibiting the Sodium/Hydrogen Exchanger
Article Snippet: .. First-strand cDNA was synthesized from 1 μg of total RNA using the LunaScript RT SuperMix Kit (New England Biolabs). .. Quantitative polymerase chain reaction (PCR) was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) on a 7500 Fast Real-Time PCR System (Thermo Fisher Scientific).

Polymerase Chain Reaction:

Article Title: Persistent SARS-CoV-2 infection in SCID rats reflects immune vulnerability of the upper airway and trachea.
Article Snippet: .. Viral cDNA synthesis was performed using the Q5 Hot Start DNA Polymerase (New England BioLabs) and the Luna Script RT Super Mix Kit (New England BioLabs), followed by multiplex PCR using ARTIC-N5 primers (used in two separate primer pools). .. These DNA fragments were prepared as libraries using the QIAseq FX DNA Library Kit (Qiagen) and sequenced on an iSeq 100 and MiSeq (Illumina).

Recombinant:

Article Title: Direct cell-to-cell transmission of retrotransposons.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat polyclonal anti-412 Gag raised against peptide MLKNDDKTNNAADSE Gift from E. Brasset N/A Rabbit polyclonal anti-412 Gag raised against peptide MLKNDDKTNNAADSE This study N/A Rabbit polyclonal anti-412 sORF1 raised against recombinant protein This study N/A Rabbit polyclonal anti-412 sORF2-N raised against peptide DTKTVDSTANVINKVEVQNAD This study N/A Rabbit polyclonal anti-412 sORF2-C raised against peptide LKKRYRSTANGIDNI This study N/A Rabbit polyclonal anti-Mack Gag raised against peptide SLSYNPTMPDEQSVPE This study N/A Rat monoclonal α-HA Roche Cat# 11867423001 ROAHAHA; RRID: AB_390918 Mouse monoclonal α-armadillo DSHB Cat# N2 7A1; RRID: AB_528089 Rat monoclonal α-vasa DSHB Cat# anti-vasa; RRID: AB_760351 Goat anti-rabbit IgG, 6 nm gold Aurion Cat# 806.011; RRID: AB_2732799 Chemicals, peptides, and recombinant proteins TRIzol Reagent Invitrogen Cat #15596026 Alexa Fluor 488 phalloidin Invitrogen Cat #A12379 Dynabeads Oligo(dT)25 Thermo Fisher Scientific Cat #61002 ProLong Diamond mounting medium Invitrogen Cat #P36965 Lunascript RT SuperMix kit NEB Cat #E3010 Enduren Live Cell Substrate Promega Cat #E6481 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Critical commercial assays RCC25 RNA cleanup kit Zymo Research Cat #R1017 ONT RNA002 Direct RNA Library Prep Kit Oxford Nanopore Technologies SQK-RNA002 NEBNext Ultra II Directional RNA library prep NEB Cat #E7760 Cell Line Nucleofector kit V Amaxa Biosystems VCA-1003 Deposited data Long-read direct RNA sequencing (ONT) from D. melanogaster ovaries and OSCs with somatic piRNA knockdown This study GEO: GSE292040 Poly(A) Illumina RNA-sequencing from D. melanogaster flamΔ412-St2 ovaries This study GEO: GSE292041 Small RNA-seq from flamΔ412-St2 ovaries This study GEO: GSE292044 Mass spectrometry proteomics data of ovaries and OSCs with somatic piRNA knockdown This study PRIDE: PXD061763 Electron tomogram of invasive protrusion in flamΔ412-St2 This study EMDB: EMD-53238 Experimental models: Cell lines Ovarian somatic cells (OSCs) Saito et al.73 RRID: CVCL_IY73 HEK293FT with Dual Split Proteins (DSP) J. Gohda; Kondo et al.54 pCMV-DSP1-7 and pCMV-DSP-8-11 Experimental models: Organisms/strains D. littoralis Kilpisj€arvi 1 C. Förster N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e11, December 10, 2026 e1 Article ..

RNA Sequencing:

Knockdown:

Mass Spectrometry:

Purification:

Article Title: Chondroitin sulfate shapes the unfolded protein response in osteosarcoma to sustain cancer cell survival during ER stress.
Article Snippet: After the treatments ended, RNA was extracted from MNNG cells using the Monarch Total RNA Miniprep Kit (New England Biolabs #T2010S) as per manufacturer’s protocol. .. Purified RNA was quantified using ThemoFisher’s NanoDrop Spectrophotometer and 1 μg RNA was reversetranscribed into cDNA using the LunaScript RT SuperMix Kit (New England Biolabs, #E3010L) in a 20 μL reaction according to manufacturer’s protocol (reaction: 2 min at 25°C, 10 min at 55°C, 1 min at 95°C). .. The resulting cDNA was diluted and amplified in a 10 μL reaction using Luna Universal qPCR Master Mix (New England Biolabs #M3003L) and gene-specific forward and reverse primers (0.6 μM) for 45 cycles in the ViiA 7 Real-Time PCR system (Applied biosystems, Thermo Fisher) according to manufacturer’s protocol.

Spectrophotometry:

Generated:

Article Title: Myelin sheath lengths in the central nervous system scale to axon diameter via oligodendroglial Piezo1
Article Snippet: DNase IXT (New England Biolabs) was used for in-column DNase treatment. .. cDNA generated with the LunaScript RT SuperMix Kit (New England Biolabs). ..

Extraction:

Article Title: Loss of CsrA in Acinetobacter baumannii leads to lethal dysregulation of alanine metabolism.
Article Snippet: RNA was extracted from two mL of culture using the Direct- zol RNA Miniprep kit (Zymo Research, cat. no. R2052) following the manufacturer’s recommendations. .. After extraction, one μg of RNA was converted to cDNA using LunaScript RT SuperMix (New England Biolabs, cat. no. E3010) as recommended by the manufacturer. .. Prior to use in qPCR reactions, the cDNA was diluted 1:10. qPCR reactions were performed using iTaq Universal SYBR Green Supermix (BioRad, cat. no. 1725122), and samples were run with technical triplicate reactions.

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