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genotyping method (Jackson Laboratory)

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Jackson Laboratory genotyping method
The specific set of primers designed for <t>PS1</t> gene generated a 231 bp PCR product in three different DNA samples tested whose genotypes were determined using the classical method (+/+, +/KI, KI/KI). PCR was performed in triplicate for each sample. No PCR product was detected in the negative control lane (T−).

The specific set of primers designed for <t>PS1</t> gene generated a 231 bp PCR product in three different DNA samples tested whose genotypes were determined using the classical method (+/+, +/KI, KI/KI). PCR was performed in triplicate for each sample. No PCR product was detected in the negative control lane (T−).

Genotyping Method, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genotyping+methods/genotyping+method/pmc02789286-264-4-16
Average 90 stars, based on 1 article reviews

genotyping method - by Bioz Stars, 2026-11

90/100 stars

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1) Product Images from "A new and simple approach for genotyping Alzheimer's disease presenilin-1 mutant knockin mice"

Article Title: A new and simple approach for genotyping Alzheimer's disease presenilin-1 mutant knockin mice

Journal:

doi: 10.1016/j.jneumeth.2009.05.009

The specific set of primers designed for PS1 gene generated a 231 bp PCR product in three different DNA samples tested whose genotypes were determined using the classical method (+/+, +/KI, KI/KI). PCR was performed in triplicate for each sample. No PCR product was detected in the negative control lane (T−).

Figure Legend Snippet: The specific set of primers designed for PS1 gene generated a 231 bp PCR product in three different DNA samples tested whose genotypes were determined using the classical method (+/+, +/KI, KI/KI). PCR was performed in triplicate for each sample. No PCR product was detected in the negative control lane (T−).

Techniques Used: Generated, Negative Control

Photomicrograph of a polyacrylamide gel of SSCP electrophoresis. Migration was stopped when the loading dye had totally migrated outside of the gel (i.e. overnight). The lower part of the gel was cut off. Arrows indicate targeted specific bands. The upper band represents wild-type allele and the lower band the mutated allele KI of PS1

Figure Legend Snippet: Photomicrograph of a polyacrylamide gel of SSCP electrophoresis. Migration was stopped when the loading dye had totally migrated outside of the gel (i.e. overnight). The lower part of the gel was cut off. Arrows indicate targeted specific bands. The upper band represents wild-type allele and the lower band the mutated allele KI of PS1

Techniques Used: Electrophoresis, Migration

Efficiency of SSCP analysis for genotyping Description of the tails (samples) tested corresponding to the number of lane and the genotypes obtained by classical PCR in order to confirm the efficiency of SSCP method. In total, 33 fragments of tails were analysed from the three genotypes blind to the tester: 7 fragments from +/+ mice among which 6 were duplicated; 4 fragments from +/KI mice among which 4 were duplicated and 6 fragments from KI/KI mice among which 6 were duplicated. The efficiency of SSCP method for genotyping was clearly confirmed (100% match).

Figure Legend Snippet: Efficiency of SSCP analysis for genotyping Description of the tails (samples) tested corresponding to the number of lane and the genotypes obtained by classical PCR in order to confirm the efficiency of SSCP method. In total, 33 fragments of tails were analysed from the three genotypes blind to the tester: 7 fragments from +/+ mice among which 6 were duplicated; 4 fragments from +/KI mice among which 4 were duplicated and 6 fragments from KI/KI mice among which 6 were duplicated. The efficiency of SSCP method for genotyping was clearly confirmed (100% match).

Techniques Used: Polymerase Chain Reaction

Transgenic Assay:

Article Title: Attenuation of Spatial Memory in 5xFAD Mice by Halting Cholinesterases, Oxidative Stress and Neuroinflammation Using a Cyclopentanone Derivative
Article Snippet: .. The genotyping methodology specified by Jackson Lab for strain transgenic mice was followed with minor modifications. ..

other:

Article Title: ATM protein is located on presynaptic vesicles and its deficit leads to failures in synaptic plasticity
Article Snippet: The ATM mouse colony 129S6/SvEvTac- Atm tm1Awb /J was purchased from Jackson Laboratory and maintained in the animal facilities at Rutgers University.

Article Title: Cyclopentanone Derivative Attenuates Memory Impairment by Inhibiting Amyloid Plaques Formation in the 5xFAD Mice
Article Snippet: Methodology of genotyping as specified by Jackson Lab U.S. for strain transgenic mice was followed with slight modifications [ ].

Article Title: Retinal Pigmented Epithelial Cells Obtained from Human Induced Pluripotent Stem Cells Possess Functional Visual Cycle Enzymes in Vitro and in Vivo
Article Snippet: All mice were genotyped by established methods provided by the Jackson Laboratory.

Article Title: A new and simple approach for genotyping Alzheimer's disease presenilin-1 mutant knockin mice
Article Snippet: The classical method for genotyping PS1 mice, described by Guo et al. (1999) and recommended by the Jackson Laboratories is a multistep method.

Article Title: Cyclin-Dependent Kinase Inhibitor 1a (p21) Modulates Response to Cocaine and Motivated Behaviors
Article Snippet: All mice were genotyped for p21 genetic status based on the method suggested by the Jackson Laboratory.

Article Title: A novel co-culture model for investigation of the effects of LPS-induced macrophage-derived cytokines on brain endothelial cells.
Article Snippet: The genotyping for the APPswe/ PS1dE9 transgenes was performed by the PCR-based method provided by the Jackson Lab. B6.

Article Title: PLX3397, a CSF1 receptor inhibitor, limits allotransplantation-induced vascular remodelling.
Article Snippet: Methods for genotyping the mice were performed according to protocols established in the Jackson laboratory.