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g418  (Thermo Fisher)


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    Structured Review

    Thermo Fisher g418
    (A) Mouse embryonal P19 cells were stably transfected with Myo6 expression vector, followed by selection by <t>G418</t> and subsequent determination of Myo6 mRNA expression with RT-PCR analysis. Several clones of stable transfectants were cultured with ATRA for 4 days for subsequent (B) micrographic observation and (C) MTT reduction determination. Each value represents the mean ± S.E. in 4 different experiments. *P<0.05, **P<0.01, significantly different from the control value in stable EV transfectants.
    G418, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+expression+specificity+analysis+single+cell+transcriptomic+data/G418+DISULFATE+CELL+CULTURE+RE/pmc03656852-45-23-24
    Average 99 stars, based on 1 article reviews
    g418 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Myosin VI Reduces Proliferation, but Not Differentiation, in Pluripotent P19 Cells"

    Article Title: Myosin VI Reduces Proliferation, but Not Differentiation, in Pluripotent P19 Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063947

    (A) Mouse embryonal P19 cells were stably transfected with Myo6 expression vector, followed by selection by G418 and subsequent determination of Myo6 mRNA expression with RT-PCR analysis. Several clones of stable transfectants were cultured with ATRA for 4 days for subsequent (B) micrographic observation and (C) MTT reduction determination. Each value represents the mean ± S.E. in 4 different experiments. *P<0.05, **P<0.01, significantly different from the control value in stable EV transfectants.
    Figure Legend Snippet: (A) Mouse embryonal P19 cells were stably transfected with Myo6 expression vector, followed by selection by G418 and subsequent determination of Myo6 mRNA expression with RT-PCR analysis. Several clones of stable transfectants were cultured with ATRA for 4 days for subsequent (B) micrographic observation and (C) MTT reduction determination. Each value represents the mean ± S.E. in 4 different experiments. *P<0.05, **P<0.01, significantly different from the control value in stable EV transfectants.

    Techniques Used: Stable Transfection, Transfection, Expressing, Plasmid Preparation, Selection, Reverse Transcription Polymerase Chain Reaction, Clone Assay, Cell Culture, Control

    Related Articles

    Stable Transfection:

    Article Title: Drosophila Abi maintains blood cell homeostasis by promoting clathrin-mediated endocytosis of Notch
    Article Snippet: For expression in Escherichia coli , a cDNA encoding NICD (amino acid residues 1765–2703) was PCR-amplified from genomic DNA isolated from UAS-N transgenic flies and cloned into the 6xHis tag vector pCold-TF (pCold-His6-TF, Takara) to produce pCold-His 6 -TF-NICD . .. S2 cells stably expressing the Myc-Ser fusion under the control of an actin promoter (S2S cells) were generated by transfecting the Ac5-Myc-Ser-STABLE1-Neo vector and selecting cells in 2 mg/ml G418 (Invitrogen) as previously described ( ). .. These cells were maintained at 25°C in Shields and Sang M3 Insect Medium (USBiological Life Sciences) supplemented with 10% heat-inactivated (30 min, 56°C) fetal bovine serum, 1 g/liter yeast extract, 2.5 g/liter Bacto-peptone, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco).

    Expressing:

    Article Title: Drosophila Abi maintains blood cell homeostasis by promoting clathrin-mediated endocytosis of Notch
    Article Snippet: For expression in Escherichia coli , a cDNA encoding NICD (amino acid residues 1765–2703) was PCR-amplified from genomic DNA isolated from UAS-N transgenic flies and cloned into the 6xHis tag vector pCold-TF (pCold-His6-TF, Takara) to produce pCold-His 6 -TF-NICD . .. S2 cells stably expressing the Myc-Ser fusion under the control of an actin promoter (S2S cells) were generated by transfecting the Ac5-Myc-Ser-STABLE1-Neo vector and selecting cells in 2 mg/ml G418 (Invitrogen) as previously described ( ). .. These cells were maintained at 25°C in Shields and Sang M3 Insect Medium (USBiological Life Sciences) supplemented with 10% heat-inactivated (30 min, 56°C) fetal bovine serum, 1 g/liter yeast extract, 2.5 g/liter Bacto-peptone, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco).

    Control:

    Article Title: Drosophila Abi maintains blood cell homeostasis by promoting clathrin-mediated endocytosis of Notch
    Article Snippet: For expression in Escherichia coli , a cDNA encoding NICD (amino acid residues 1765–2703) was PCR-amplified from genomic DNA isolated from UAS-N transgenic flies and cloned into the 6xHis tag vector pCold-TF (pCold-His6-TF, Takara) to produce pCold-His 6 -TF-NICD . .. S2 cells stably expressing the Myc-Ser fusion under the control of an actin promoter (S2S cells) were generated by transfecting the Ac5-Myc-Ser-STABLE1-Neo vector and selecting cells in 2 mg/ml G418 (Invitrogen) as previously described ( ). .. These cells were maintained at 25°C in Shields and Sang M3 Insect Medium (USBiological Life Sciences) supplemented with 10% heat-inactivated (30 min, 56°C) fetal bovine serum, 1 g/liter yeast extract, 2.5 g/liter Bacto-peptone, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco).

    Generated:

    Article Title: Drosophila Abi maintains blood cell homeostasis by promoting clathrin-mediated endocytosis of Notch
    Article Snippet: For expression in Escherichia coli , a cDNA encoding NICD (amino acid residues 1765–2703) was PCR-amplified from genomic DNA isolated from UAS-N transgenic flies and cloned into the 6xHis tag vector pCold-TF (pCold-His6-TF, Takara) to produce pCold-His 6 -TF-NICD . .. S2 cells stably expressing the Myc-Ser fusion under the control of an actin promoter (S2S cells) were generated by transfecting the Ac5-Myc-Ser-STABLE1-Neo vector and selecting cells in 2 mg/ml G418 (Invitrogen) as previously described ( ). .. These cells were maintained at 25°C in Shields and Sang M3 Insect Medium (USBiological Life Sciences) supplemented with 10% heat-inactivated (30 min, 56°C) fetal bovine serum, 1 g/liter yeast extract, 2.5 g/liter Bacto-peptone, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco).

    Polymerase Chain Reaction:

    Article Title: Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?
    Article Snippet: All the S. cerevisiae strains generated in this work were constructed as follows: firstly, DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase, Nzytech) with specific oligonucleotides (listed in Table ) using yeast genomic DNA or plasmid DNA. .. The resulting PCR products were introduced in S. cerevisiae cells, by following the polyethylene glycol (PEG)/lithium acetate (LiAc) protocol [ ], and the resulting transformants were selected on geneticin (G418 disulfate, Alfa Aesar; J62671) or hygromycin B (Alfa Aesar; J60681) according to the resistance (R) marker present (geneticin for kan R and hygromycin for hgh R). ..

    Marker:

    Article Title: Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?
    Article Snippet: All the S. cerevisiae strains generated in this work were constructed as follows: firstly, DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase, Nzytech) with specific oligonucleotides (listed in Table ) using yeast genomic DNA or plasmid DNA. .. The resulting PCR products were introduced in S. cerevisiae cells, by following the polyethylene glycol (PEG)/lithium acetate (LiAc) protocol [ ], and the resulting transformants were selected on geneticin (G418 disulfate, Alfa Aesar; J62671) or hygromycin B (Alfa Aesar; J60681) according to the resistance (R) marker present (geneticin for kan R and hygromycin for hgh R). ..

    Transduction:

    Article Title: ZEB1, a novel junctional adhesion molecule A regulator, impacts sensitivity of pancreatic cancer-associated fibroblasts to reovirus
    Article Snippet: .. Clonal ZEB1 KO cells were transduced with this vector and selected and subsequently cultured with puromycin (2 μg/mL; Sigma-Aldrich) or G418 (400 μg/mL; Thermo Fisher Scientific). ..

    Plasmid Preparation:

    Article Title: ZEB1, a novel junctional adhesion molecule A regulator, impacts sensitivity of pancreatic cancer-associated fibroblasts to reovirus
    Article Snippet: .. Clonal ZEB1 KO cells were transduced with this vector and selected and subsequently cultured with puromycin (2 μg/mL; Sigma-Aldrich) or G418 (400 μg/mL; Thermo Fisher Scientific). ..

    Cell Culture:

    Article Title: ZEB1, a novel junctional adhesion molecule A regulator, impacts sensitivity of pancreatic cancer-associated fibroblasts to reovirus
    Article Snippet: .. Clonal ZEB1 KO cells were transduced with this vector and selected and subsequently cultured with puromycin (2 μg/mL; Sigma-Aldrich) or G418 (400 μg/mL; Thermo Fisher Scientific). ..

    Recombinant:

    Article Title: A human CAGinSTEM platform for decoding HTT repeats’ somatic instability links CAG interruption to HD pathology in neurons
    Article Snippet: HiFi Cas9 Nuclease V3 (IDT # 1081060) and sgRNA RNP complex using Nucleofector kit (Lonza), in Nucleofector II (Amaxa biosystems) using B-016 program. .. After nucleofection, cells were plated in mTeSR media with 10 μM RI (Y-27632, provided by CHDI foundation) and 1 μM of SCR7 (TOCRIS) for 24 h. Two days after nucleofection, cells were daily treated with 0.1 mg/mL G418 (ThermoFisher) for 5 days to select and isolate recombinant cells. ..



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