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Arraystar inc hybridization solution
Hybridization Solution, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/g2565ba+microarray+scanner/hybridization+solution/pm29794463-83-5-25
Average 90 stars, based on 1 article reviews
hybridization solution - by Bioz Stars, 2026-11
90/100 stars

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Hybridization:

Article Title: Expression profile of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Article Snippet: .. Additionally, 50 μl of hybridization solution was injected into the spacer glass slide, assembled on the Arraystar Human circRNA V2 (8 × 15 K) chip (Arraystar, USA), and incubated in an Agilent hybridization oven at 65 °C for 17 h. After washing the slides, scan the array with G2505C gene scanner (Agilent, USA). .. The sequencing process of circRNA chip was carried out according to the protocol of KangChen Bio-tech, Shanghai, China.

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 °C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer's disease patients.
Article Snippet: Expression of circular RNA (circRNA), a class of noncoding RNAs that regulates gene expression, is altered in Alzheimer’s disease.. This study profiled differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from five patients with Alzheimer’s disease compared to healthy controls using circRNA microarrays.. We identified a total of 4060 differentially expressed circRNAs (1990 upregulated and 2070 downregulated) in Alzheimer’s disease patients.

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis.
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 ◦C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 ◦C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Injection:

Article Title: Expression profile of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Article Snippet: .. Additionally, 50 μl of hybridization solution was injected into the spacer glass slide, assembled on the Arraystar Human circRNA V2 (8 × 15 K) chip (Arraystar, USA), and incubated in an Agilent hybridization oven at 65 °C for 17 h. After washing the slides, scan the array with G2505C gene scanner (Agilent, USA). .. The sequencing process of circRNA chip was carried out according to the protocol of KangChen Bio-tech, Shanghai, China.

Incubation:

Article Title: Expression profile of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Article Snippet: .. Additionally, 50 μl of hybridization solution was injected into the spacer glass slide, assembled on the Arraystar Human circRNA V2 (8 × 15 K) chip (Arraystar, USA), and incubated in an Agilent hybridization oven at 65 °C for 17 h. After washing the slides, scan the array with G2505C gene scanner (Agilent, USA). .. The sequencing process of circRNA chip was carried out according to the protocol of KangChen Bio-tech, Shanghai, China.

other:

Article Title: hsa_circRNA_100873 upregulation is associated with increased lymphatic metastasis of esophageal squamous cell carcinoma.
Article Snippet: Next, 25 μl 2x hybridization buffer (Arraystar Super RNA Tag kit; Arraystar) was added to dilute the labeled circRNA samples.

Article Title: hsa_circRNA_100873 upregulation is associated with increased lymphatic metastasis of esophageal squamous cell carcinoma
Article Snippet: Following the protocol of the Arraystar Super RNA Tag kit, enriched circRNAs were amplified and transcribed into fluorescent circRNA utilizing a random priming method.

Labeling:

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 °C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer's disease patients.
Article Snippet: Expression of circular RNA (circRNA), a class of noncoding RNAs that regulates gene expression, is altered in Alzheimer’s disease.. This study profiled differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from five patients with Alzheimer’s disease compared to healthy controls using circRNA microarrays.. We identified a total of 4060 differentially expressed circRNAs (1990 upregulated and 2070 downregulated) in Alzheimer’s disease patients.

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis.
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 ◦C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 ◦C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Blocking Assay:

Article Title: Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer's disease patients.
Article Snippet: Expression of circular RNA (circRNA), a class of noncoding RNAs that regulates gene expression, is altered in Alzheimer’s disease.. This study profiled differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from five patients with Alzheimer’s disease compared to healthy controls using circRNA microarrays.. We identified a total of 4060 differentially expressed circRNAs (1990 upregulated and 2070 downregulated) in Alzheimer’s disease patients.



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