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fluoroshield with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield with dapi
    Fluoroshield With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshieldtm+with+dapi/DAPI/pm40022162-106-16-20
    Average 99 stars, based on 32901 article reviews
    fluoroshield with dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis
    Article Snippet: NIH‐3T3 cells, RAW264.7 cells, or 4T1/MCF7 cells treated with 40 ng mL −1 rCXCL10 were seeded onto the coverslips and cultured for 72 h. Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.2% Triton X‐100 (Solarbio, T8200) for 5 min, and blocked with 5% BSA (Solarbio, A8850) for 2 h. For tissue sections, slides underwent deparaffinization, rehydration, and blocking with 5% BSA for 2 h. Both coverslips and tissue sections were incubated overnight at 4°C with primary antibodies against α‐SMA (Affinity, AF1032), CD206 (Affinity, DF4149), or β‐Catenin (Proteintech, PRO‐CY‐50WY). .. After three washes, samples were incubated for 1 h at room temperature in the dark with Fluor488‐conjugated secondary antibody (Immunoway, RS32109), counterstained with DAPI (Beyotime, C1005) for 3 min, and mounted with antifade medium (Abbkine, BMU104). ..

    Staining:

    Article Title: Targeting REV7 confers cervical cancer cell radiosensitivity and ferroptosis by negatively regulating SFXN1
    Article Snippet: The cells were incubated with the antibody against γH2AX (1:1000, Abcam, Cambridge, UK) overnight at 4 °C and then incubated with Cy3-labeled goat anti-mouse IgG (1:1000, Beyotime) for 30 min at 37 °C in the dark. .. Nuclei were stained with DAPI (Beyotime) for 5 min. .. Finally, images were acquired using an FV1200 Olympus confocal microscope (Olympus, Tokyo, Japan) with γH2AX (Excitation wavelength: 550 nm, emission wavelength: 570 nm; exposure time: 1000 ms) and DAPI (Excitation wavelength: 358 nm, emission wavelength: 461 nm; exposure time: 1000 ms).

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark. .. Finally, the fluorescence images of the stained cells were obtained using a confocal laser scanning microscope (Leica TCS SP8, Leica Microsystems, Wetzlar, Germany) and analyzed with ImageJ software.

    Lysis:

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: Mouse ELISA kits for quantifying IL‐6, IL‐1β, TNF‐α, and IFN‐β were supplied by Beijing Solarbio Science & Technology Co., Ltd. (China). .. DAPI, PMSF, and RIPA lysis buffer were acquired from Beyotime Biotechnology (China). .. Antibodies targeting phospho‐TBK1 (Ser172, #5483), phospho‐STING (Ser365, #72971), total TBK1 (#38066), and total STING (#13647) were purchased from Cell Signaling Technology (CST) via Beijing Chengzhi Kewei Biotechnology Co., Ltd. (China).

    Fluorescence:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Microscopy:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..



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    Image Search Results


    Schematic diagram for primary HONCs culture establishment. (a) An excised specimen from human olfactory neuroepithelium. (b) Immunohistochemical stain with OMP (green), CK5 (red), and DAPI (blue) represents mature OSNs, basal cells, and nuclei, respectively (top). β4-tubulin (red) and DAPI (blue) immunohistochemical markers were to rule out respiratory epithelium (scale bar = 100 μm). (c) Schematic showing HONCs maturation process from progenitor cells to mature neuronal cells. (d) Time course of the experimental design with accompanying microscopy indicating cell migration from explants (scale bar = 1 mm).

    Journal: Regenerative Therapy

    Article Title: Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1

    doi: 10.1016/j.reth.2024.10.014

    Figure Lengend Snippet: Schematic diagram for primary HONCs culture establishment. (a) An excised specimen from human olfactory neuroepithelium. (b) Immunohistochemical stain with OMP (green), CK5 (red), and DAPI (blue) represents mature OSNs, basal cells, and nuclei, respectively (top). β4-tubulin (red) and DAPI (blue) immunohistochemical markers were to rule out respiratory epithelium (scale bar = 100 μm). (c) Schematic showing HONCs maturation process from progenitor cells to mature neuronal cells. (d) Time course of the experimental design with accompanying microscopy indicating cell migration from explants (scale bar = 1 mm).

    Article Snippet: Sections were washed with PBS, and then mounted with FluoroshieldTM with DAPI (GTX30920, Genetex, CA, USA) for further observation.

    Techniques: Immunohistochemical staining, Staining, Microscopy, Migration

    Immunofluorescence of signal transduction protein for cultures with or without orexin-A (1 ng/mL). The cells stained with protein marker (green), (a) ADCY3 and (b) Golf protein markers are used to identify olfactory signal transduction. Cellular cytoskeletal shape (F-actin) and nuclei are visualized with staining using phalloidin-ifluor 555 (in red) and DAPI (in blue), respectively (scale bar = 100 μm).

    Journal: Regenerative Therapy

    Article Title: Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1

    doi: 10.1016/j.reth.2024.10.014

    Figure Lengend Snippet: Immunofluorescence of signal transduction protein for cultures with or without orexin-A (1 ng/mL). The cells stained with protein marker (green), (a) ADCY3 and (b) Golf protein markers are used to identify olfactory signal transduction. Cellular cytoskeletal shape (F-actin) and nuclei are visualized with staining using phalloidin-ifluor 555 (in red) and DAPI (in blue), respectively (scale bar = 100 μm).

    Article Snippet: Sections were washed with PBS, and then mounted with FluoroshieldTM with DAPI (GTX30920, Genetex, CA, USA) for further observation.

    Techniques: Immunofluorescence, Transduction, Staining, Marker