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fluorescent probe dil staining solution  (Beyotime)


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    Beyotime fluorescent probe dil staining solution
    Fluorescent Probe Dil Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+probe+dapi/DAPI+Staining+Solution/pmc09179371-32-6-14
    Average 99 stars, based on 2060 article reviews
    fluorescent probe dil staining solution - by Bioz Stars, 2026-10
    99/100 stars

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    Bicinchoninic Acid Protein Assay:

    Article Title: The host protein SSR4 mediates PRRSV-induced endoplasmic reticulum stress via interaction with Nsp2.
    Article Snippet: The following primary antibodies were used: anti-β-actin (Proteintech, 66009-1-Ig), anti-Calnexin (Proteintech, 10427-2-AP), anti-SSR1 (Proteintech, 10583-1-AP), anti-SSR2 (Proteintech, 10278-1-AP), anti-SSR3 (Proteintech, 30851-1-AP), anti-SSR4 (Proteintech, 11655-2-AP), anti-ATF4 (Proteintech, 10835-1-AP), anti-EDEM1 (Proteintech, 26226-1-AP), anti-GRP78 (Beyotime, AF0171), anti-ATF6 (Proteintech, 24169-1-AP), anti-Phospho-eIF2 alpha (Cell Signaling Technology, #3398), anti-eIF2 alpha (Cell Signaling Technol ogy, #5324), anti-Phospho-IRE1 (HUABIO, HA721980), anti-IRE1 (HUABIO, HA723225), anti-Puromycin (ABclonal, A21205), monoclonal anti-PRRSV N protein (generated in-house), and monoclonal anti-PRRSV Nsp2 (generated in-house). .. Key commercial reagents included tunicamycin (Beyotime, SC0393), DTT (Solarbio, 3483-12-3), 4-phenyl butyric acid (MCE, HY-A0281), MK-28 (AbMole, M56016), puromycin (MCE, HY-B1743), cycloheximide (MCE, HY-12320), BCA Protein Assay Kit (Thermo Fisher, 23225), Lipo fectamine 3000 (Thermo Fisher, L3000015), Lipofectamine RNAiMAX (Thermo Fisher, 13778075), ReverTra Ace qPCR RT Kit (TOYOBO, NLQ-101), KOD qPCR SYBR Mix (TOYOBO, QKD-201T), tunicamycin (MedChemExpress, HY-A0098), RIPA lysis buffer (Beyotime, P0013C), DAPI staining solution (Beyotime, C1006), and polyethylenimine (PEI; Yeasen). ..

    Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
    Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).

    Real-time Polymerase Chain Reaction:

    Article Title: The host protein SSR4 mediates PRRSV-induced endoplasmic reticulum stress via interaction with Nsp2.
    Article Snippet: The following primary antibodies were used: anti-β-actin (Proteintech, 66009-1-Ig), anti-Calnexin (Proteintech, 10427-2-AP), anti-SSR1 (Proteintech, 10583-1-AP), anti-SSR2 (Proteintech, 10278-1-AP), anti-SSR3 (Proteintech, 30851-1-AP), anti-SSR4 (Proteintech, 11655-2-AP), anti-ATF4 (Proteintech, 10835-1-AP), anti-EDEM1 (Proteintech, 26226-1-AP), anti-GRP78 (Beyotime, AF0171), anti-ATF6 (Proteintech, 24169-1-AP), anti-Phospho-eIF2 alpha (Cell Signaling Technology, #3398), anti-eIF2 alpha (Cell Signaling Technol ogy, #5324), anti-Phospho-IRE1 (HUABIO, HA721980), anti-IRE1 (HUABIO, HA723225), anti-Puromycin (ABclonal, A21205), monoclonal anti-PRRSV N protein (generated in-house), and monoclonal anti-PRRSV Nsp2 (generated in-house). .. Key commercial reagents included tunicamycin (Beyotime, SC0393), DTT (Solarbio, 3483-12-3), 4-phenyl butyric acid (MCE, HY-A0281), MK-28 (AbMole, M56016), puromycin (MCE, HY-B1743), cycloheximide (MCE, HY-12320), BCA Protein Assay Kit (Thermo Fisher, 23225), Lipo fectamine 3000 (Thermo Fisher, L3000015), Lipofectamine RNAiMAX (Thermo Fisher, 13778075), ReverTra Ace qPCR RT Kit (TOYOBO, NLQ-101), KOD qPCR SYBR Mix (TOYOBO, QKD-201T), tunicamycin (MedChemExpress, HY-A0098), RIPA lysis buffer (Beyotime, P0013C), DAPI staining solution (Beyotime, C1006), and polyethylenimine (PEI; Yeasen). ..

    Lysis:

    Article Title: The host protein SSR4 mediates PRRSV-induced endoplasmic reticulum stress via interaction with Nsp2.
    Article Snippet: The following primary antibodies were used: anti-β-actin (Proteintech, 66009-1-Ig), anti-Calnexin (Proteintech, 10427-2-AP), anti-SSR1 (Proteintech, 10583-1-AP), anti-SSR2 (Proteintech, 10278-1-AP), anti-SSR3 (Proteintech, 30851-1-AP), anti-SSR4 (Proteintech, 11655-2-AP), anti-ATF4 (Proteintech, 10835-1-AP), anti-EDEM1 (Proteintech, 26226-1-AP), anti-GRP78 (Beyotime, AF0171), anti-ATF6 (Proteintech, 24169-1-AP), anti-Phospho-eIF2 alpha (Cell Signaling Technology, #3398), anti-eIF2 alpha (Cell Signaling Technol ogy, #5324), anti-Phospho-IRE1 (HUABIO, HA721980), anti-IRE1 (HUABIO, HA723225), anti-Puromycin (ABclonal, A21205), monoclonal anti-PRRSV N protein (generated in-house), and monoclonal anti-PRRSV Nsp2 (generated in-house). .. Key commercial reagents included tunicamycin (Beyotime, SC0393), DTT (Solarbio, 3483-12-3), 4-phenyl butyric acid (MCE, HY-A0281), MK-28 (AbMole, M56016), puromycin (MCE, HY-B1743), cycloheximide (MCE, HY-12320), BCA Protein Assay Kit (Thermo Fisher, 23225), Lipo fectamine 3000 (Thermo Fisher, L3000015), Lipofectamine RNAiMAX (Thermo Fisher, 13778075), ReverTra Ace qPCR RT Kit (TOYOBO, NLQ-101), KOD qPCR SYBR Mix (TOYOBO, QKD-201T), tunicamycin (MedChemExpress, HY-A0098), RIPA lysis buffer (Beyotime, P0013C), DAPI staining solution (Beyotime, C1006), and polyethylenimine (PEI; Yeasen). ..

    Staining:

    Article Title: The host protein SSR4 mediates PRRSV-induced endoplasmic reticulum stress via interaction with Nsp2.
    Article Snippet: The following primary antibodies were used: anti-β-actin (Proteintech, 66009-1-Ig), anti-Calnexin (Proteintech, 10427-2-AP), anti-SSR1 (Proteintech, 10583-1-AP), anti-SSR2 (Proteintech, 10278-1-AP), anti-SSR3 (Proteintech, 30851-1-AP), anti-SSR4 (Proteintech, 11655-2-AP), anti-ATF4 (Proteintech, 10835-1-AP), anti-EDEM1 (Proteintech, 26226-1-AP), anti-GRP78 (Beyotime, AF0171), anti-ATF6 (Proteintech, 24169-1-AP), anti-Phospho-eIF2 alpha (Cell Signaling Technology, #3398), anti-eIF2 alpha (Cell Signaling Technol ogy, #5324), anti-Phospho-IRE1 (HUABIO, HA721980), anti-IRE1 (HUABIO, HA723225), anti-Puromycin (ABclonal, A21205), monoclonal anti-PRRSV N protein (generated in-house), and monoclonal anti-PRRSV Nsp2 (generated in-house). .. Key commercial reagents included tunicamycin (Beyotime, SC0393), DTT (Solarbio, 3483-12-3), 4-phenyl butyric acid (MCE, HY-A0281), MK-28 (AbMole, M56016), puromycin (MCE, HY-B1743), cycloheximide (MCE, HY-12320), BCA Protein Assay Kit (Thermo Fisher, 23225), Lipo fectamine 3000 (Thermo Fisher, L3000015), Lipofectamine RNAiMAX (Thermo Fisher, 13778075), ReverTra Ace qPCR RT Kit (TOYOBO, NLQ-101), KOD qPCR SYBR Mix (TOYOBO, QKD-201T), tunicamycin (MedChemExpress, HY-A0098), RIPA lysis buffer (Beyotime, P0013C), DAPI staining solution (Beyotime, C1006), and polyethylenimine (PEI; Yeasen). ..

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation
    Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

    Article Title: Targeted delivery of lupeol via hyaluronic acid-modified ZIF-8 for anti-hepatic fibrosis therapy
    Article Snippet: Quantitative analysis was performed using flow cytometry (Beckman Coulter, USA). .. A DAPI Staining Solution (Beyotime Biotechnology, Shanghai, China) was used to perform the cell apoptosis shape assay on LX-2 cells according to the manufacturer's instructions. .. Cells were seeded into six-well plates at a density of 1.0 × 10 6 cells per well and cultured for 24 h. The next day, cells were treated with different media containing Lupeol, Lupeol@ZIF-8 and HA/Lupeol@ZIF-8 (60 μM lupeol), with blank and model groups included as controls, and incubated for an additional 24 h. For qualitative analysis, DAPI solution was added 24 h after treatment, and fluorescence signals were recorded using a fluorescence microscope (Olympus, Tokyo, Japan).

    Article Title: Geometry-driven immunomodulation in 3D-printed bioceramics: Negative curvature promotes macrophage M2 polarization via Ras-MAPK/HIF-1α signaling for vascularized osteogenesis
    Article Snippet: The cells types utilized in this study included: hBMSCs (No. 7500, ScienCell, USA.), Raw264.7 (CL-0190, Procell system, China), HUVECs (No. 8000, ScienCell, USA.), rBMSCs (MUBMX-01001, OriCell, USA.) and MS1(CL-0162, Procell system, China). .. The reagents used in the experiment included: H-DMEM(11965118, Gibco, USA.), α-DMEM medium(12571063, Gibco, USA.), TritonX-100(ST1723, Beyotime, China), 4 % paraformaldehyde (BL539A, Biosharp, China),FBS(A5256701, Gibco, USA.),ECM medium (Science Cell, USA.),and DAPI staining solution (C1006, Beyotime, China),BCIP/NBT(C3206, Beyotime, China), reactive oxygen species kit (S0033S, Beyotime, China), BSA (B2064, ≥98 %, Sigma-Aldrich, USA.),CD31 antibody (ab28364, Abcam, USA.), secondary anti-IGg (ab175773, Alexa Fluor® 680, Abcam, USA.), Phalloidin-iFluor 488(ab176753, Abcam, USA.), CCR7(AF5293, Bioss, China), CD206 (bsm-60761R, Bioss, China), iNOS (bs-22924R, Bioss, China), RIPA (P0013, Beyotime, China), p-ERK1/2 (AF3687, Affinity, USA.) and ERK1/2 (#AF0155, Affinity, USA), luminol detection reagent (sc-2048, Santa Cruz, USA.), GAPDH (Cat#KC-5G5, Kangchen Biotechnology, China), Trizol(15596026CN, Invitrogen, USA.), DEPC(R0601, Thermo Scientific, USA.), TBST(R017R.0000, Thermo Scientific, USA.), HIF-1a(GTX127309, GeneTex, USA.), β-Tubulin(10094-1-AP, Proteintech, UK) Adezmapimod (SB 203580, MCE, USA.) medium and Paclitaxel (99.88 %, HY-B0015R, MCE), ELISA Arg-1(E-EL-M3092, ELabSci@, China), TNF-α (E-EL-M3063, ELabSci@, China), OPN(22952-1-AP, Proteintech, UK.), F4/80 (GB11027-100, Servicebio, China) Alkaline phosphatase activity kit (P0321S, Beyotime, China), Matrigel (CLS356234, Corning, USA), Microfill MV120 (Flow tech, USA), EDTA(17892, Thermo Scientific, USA.) xylene(X112051, AR,99 %, Aladdin, China), ethanol (107-21-1, AR,99 %, Aladdin, China). .. All primers were designed and synthesized by Genema (Shanghai) Co., Ltd. 5.2.Fabrication of high-throughput array cell culture chip (HCGC) with Gaussian curvature gradient distribution. (1) Constructing models with different Gaussian curvatures: the multipipe of Grasshopper in Rhinocero software is used to regulate Gaussian curvature of the truss in the diamond framework in .

    Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
    Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).

    Article Title: Activation of the TGR5/cAMP/PKA/CREB axis in cholangiocytes mediates epithelial-mesenchymal transition and fibrosis in hepatolithiasis.
    Article Snippet: On the following day, cells were washed three times with PBS (5 minutes each), then 313 incubated at 37°C for 30 minutes with fluorescent secondary antibodies (e.g., Alexa Fluor 488/594 314 conjugates; Invitrogen, 1:500). .. After additional PBS washes, DAPI staining solution (Beyotime, 315 Cat# C1002, 1 μg/mL) was added and incubated in the dark for 3 minutes. ..

    Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation.
    Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

    ROS Assay:

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Membrane:

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Incubation:

    Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation
    Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

    Article Title: Activation of the TGR5/cAMP/PKA/CREB axis in cholangiocytes mediates epithelial-mesenchymal transition and fibrosis in hepatolithiasis.
    Article Snippet: On the following day, cells were washed three times with PBS (5 minutes each), then 313 incubated at 37°C for 30 minutes with fluorescent secondary antibodies (e.g., Alexa Fluor 488/594 314 conjugates; Invitrogen, 1:500). .. After additional PBS washes, DAPI staining solution (Beyotime, 315 Cat# C1002, 1 μg/mL) was added and incubated in the dark for 3 minutes. ..

    Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation.
    Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Geometry-driven immunomodulation in 3D-printed bioceramics: Negative curvature promotes macrophage M2 polarization via Ras-MAPK/HIF-1α signaling for vascularized osteogenesis
    Article Snippet: The cells types utilized in this study included: hBMSCs (No. 7500, ScienCell, USA.), Raw264.7 (CL-0190, Procell system, China), HUVECs (No. 8000, ScienCell, USA.), rBMSCs (MUBMX-01001, OriCell, USA.) and MS1(CL-0162, Procell system, China). .. The reagents used in the experiment included: H-DMEM(11965118, Gibco, USA.), α-DMEM medium(12571063, Gibco, USA.), TritonX-100(ST1723, Beyotime, China), 4 % paraformaldehyde (BL539A, Biosharp, China),FBS(A5256701, Gibco, USA.),ECM medium (Science Cell, USA.),and DAPI staining solution (C1006, Beyotime, China),BCIP/NBT(C3206, Beyotime, China), reactive oxygen species kit (S0033S, Beyotime, China), BSA (B2064, ≥98 %, Sigma-Aldrich, USA.),CD31 antibody (ab28364, Abcam, USA.), secondary anti-IGg (ab175773, Alexa Fluor® 680, Abcam, USA.), Phalloidin-iFluor 488(ab176753, Abcam, USA.), CCR7(AF5293, Bioss, China), CD206 (bsm-60761R, Bioss, China), iNOS (bs-22924R, Bioss, China), RIPA (P0013, Beyotime, China), p-ERK1/2 (AF3687, Affinity, USA.) and ERK1/2 (#AF0155, Affinity, USA), luminol detection reagent (sc-2048, Santa Cruz, USA.), GAPDH (Cat#KC-5G5, Kangchen Biotechnology, China), Trizol(15596026CN, Invitrogen, USA.), DEPC(R0601, Thermo Scientific, USA.), TBST(R017R.0000, Thermo Scientific, USA.), HIF-1a(GTX127309, GeneTex, USA.), β-Tubulin(10094-1-AP, Proteintech, UK) Adezmapimod (SB 203580, MCE, USA.) medium and Paclitaxel (99.88 %, HY-B0015R, MCE), ELISA Arg-1(E-EL-M3092, ELabSci@, China), TNF-α (E-EL-M3063, ELabSci@, China), OPN(22952-1-AP, Proteintech, UK.), F4/80 (GB11027-100, Servicebio, China) Alkaline phosphatase activity kit (P0321S, Beyotime, China), Matrigel (CLS356234, Corning, USA), Microfill MV120 (Flow tech, USA), EDTA(17892, Thermo Scientific, USA.) xylene(X112051, AR,99 %, Aladdin, China), ethanol (107-21-1, AR,99 %, Aladdin, China). .. All primers were designed and synthesized by Genema (Shanghai) Co., Ltd. 5.2.Fabrication of high-throughput array cell culture chip (HCGC) with Gaussian curvature gradient distribution. (1) Constructing models with different Gaussian curvatures: the multipipe of Grasshopper in Rhinocero software is used to regulate Gaussian curvature of the truss in the diamond framework in .

    Activity Assay:

    Article Title: Geometry-driven immunomodulation in 3D-printed bioceramics: Negative curvature promotes macrophage M2 polarization via Ras-MAPK/HIF-1α signaling for vascularized osteogenesis
    Article Snippet: The cells types utilized in this study included: hBMSCs (No. 7500, ScienCell, USA.), Raw264.7 (CL-0190, Procell system, China), HUVECs (No. 8000, ScienCell, USA.), rBMSCs (MUBMX-01001, OriCell, USA.) and MS1(CL-0162, Procell system, China). .. The reagents used in the experiment included: H-DMEM(11965118, Gibco, USA.), α-DMEM medium(12571063, Gibco, USA.), TritonX-100(ST1723, Beyotime, China), 4 % paraformaldehyde (BL539A, Biosharp, China),FBS(A5256701, Gibco, USA.),ECM medium (Science Cell, USA.),and DAPI staining solution (C1006, Beyotime, China),BCIP/NBT(C3206, Beyotime, China), reactive oxygen species kit (S0033S, Beyotime, China), BSA (B2064, ≥98 %, Sigma-Aldrich, USA.),CD31 antibody (ab28364, Abcam, USA.), secondary anti-IGg (ab175773, Alexa Fluor® 680, Abcam, USA.), Phalloidin-iFluor 488(ab176753, Abcam, USA.), CCR7(AF5293, Bioss, China), CD206 (bsm-60761R, Bioss, China), iNOS (bs-22924R, Bioss, China), RIPA (P0013, Beyotime, China), p-ERK1/2 (AF3687, Affinity, USA.) and ERK1/2 (#AF0155, Affinity, USA), luminol detection reagent (sc-2048, Santa Cruz, USA.), GAPDH (Cat#KC-5G5, Kangchen Biotechnology, China), Trizol(15596026CN, Invitrogen, USA.), DEPC(R0601, Thermo Scientific, USA.), TBST(R017R.0000, Thermo Scientific, USA.), HIF-1a(GTX127309, GeneTex, USA.), β-Tubulin(10094-1-AP, Proteintech, UK) Adezmapimod (SB 203580, MCE, USA.) medium and Paclitaxel (99.88 %, HY-B0015R, MCE), ELISA Arg-1(E-EL-M3092, ELabSci@, China), TNF-α (E-EL-M3063, ELabSci@, China), OPN(22952-1-AP, Proteintech, UK.), F4/80 (GB11027-100, Servicebio, China) Alkaline phosphatase activity kit (P0321S, Beyotime, China), Matrigel (CLS356234, Corning, USA), Microfill MV120 (Flow tech, USA), EDTA(17892, Thermo Scientific, USA.) xylene(X112051, AR,99 %, Aladdin, China), ethanol (107-21-1, AR,99 %, Aladdin, China). .. All primers were designed and synthesized by Genema (Shanghai) Co., Ltd. 5.2.Fabrication of high-throughput array cell culture chip (HCGC) with Gaussian curvature gradient distribution. (1) Constructing models with different Gaussian curvatures: the multipipe of Grasshopper in Rhinocero software is used to regulate Gaussian curvature of the truss in the diamond framework in .



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    2 µ m dapi fluorescent probe  (Thermo Fisher)
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    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
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    fluorescent probe 4 6 diamidino 2phenylindole dapi  (Boster Bio)
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    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
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    dna-specific fluorescent probe 4'-6-diamidino-2phenylindole (dapi)  (Boster Bio)
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    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with <t>DAPI</t> (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.
    Dna Specific Fluorescent Probe 4' 6 Diamidino 2phenylindole (Dapi), supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and DAPI (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.

    Journal: PLOS One

    Article Title: Transcranial photobiomodulation therapy with 808 nm light changes expression of genes and proteins associated with neuroprotection, neuroinflammation, oxidative stress, and Alzheimer’s disease: Whole RNA sequencing of mouse cortex and hippocampus

    doi: 10.1371/journal.pone.0326881

    Figure Lengend Snippet: (A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and DAPI (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.

    Article Snippet: The tissue slices were stained with Arginase 1 antibody, inducible nitric oxide synthase (iNOS) antibody [ ], and DAPI fluorescent probe (Thermo Fisher Scientific, USA).

    Techniques: Fluorescence, Microscopy, Staining

    Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Orally Administered Functional Polyphenol-Nanozyme-Armored Probiotics for Enhanced Amelioration of Intestinal Inflammation and Microbiota Dysbiosis.

    doi: 10.1002/advs.202411939

    Figure Lengend Snippet: Figure 6. Therapeutic efficacy of ECA@EcN in a DSS-induced mouse colitis model. A) Schematic representation of the therapeutic evaluation procedure for ECA@EcN in a DSS-induced colitis model. B) Body weight variations in mice subjected to different treatments. C) Changes in disease activity index (DAI) during treatment. D,E) Colon tissue images and corresponding quantified colon lengths after various treatments. F,G) H&E-stained histological images of colon tissues and corresponding histopathological scores. Scale bar: 100 μm. H) Serum levels of TNF-𝛼, IL-6, and IL-1𝛽measured by ELISA on day 13. I) Representative immunofluorescence images of claudin-1 (green) and ZO-1 (yellow) with DAPI (blue) nuclear counterstaining. Scale bar: 100 μm. All data are presented as the means ± SD (n = 6). Statistical analysis was performed using one-way ANOVA. *p < 0.05; ns, not significant.

    Article Snippet: 3,3′,5,5′-Tetramethylbenzidine (TMB: ST746), DCFH-DA fluorescent probe, Total SOD Assay Kit with WST-8 (Cat# S0101S), Catalase Assay Kit (Cat# S0051), Hoechst 33342 (Cat# C1028), and DAPI fluorescent probe were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

    Techniques: Activity Assay, Staining, Enzyme-linked Immunosorbent Assay