flag control (Addgene inc)

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Addgene inc flag control
Flag Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+control/FLAG-HA-pcDNA3%2E1-+(Plasmid+%2352535)/pm41838122-98-5-7
Average 95 stars, based on 116 article reviews

flag control - by Bioz Stars, 2026-11

95/100 stars

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Plasmid Preparation:

Article Title: EGR Proteins Mediate Interferon‐Independent Anti‐HSV‐1 Responses Through Viral and Host Targets
Article Snippet: .. The human Egr1 gene was inserted between the EcoRI and HindIII sites of FLAG‐HA‐pcDNA3.1‐ vector (Addgene, 52535, here referred to as pcDNA) to make pEGR1. ..

Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
Article Snippet: METTL3 (K513R) and SETD1A (R1495A) were generated 176 by a site-directed mutagenesis kit (C214-01, Vazyme). .. The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936).

Expressing:

Article Title: Functional and clinical insights into nuclear receptor variants for advancing precision diagnostics in male infertility
Article Snippet: .. The pcDNA3.1 (+) expression vectors (RRID: Addgene_52535) containing the wild type NR5A1 ( NM_004959 ; NR5A1-WT) and AR ( NM_000044 ; pcDNA3.1 (+)AR-WT) coding sequences were commercially purchased (GenScript Biotech Corp., USA). .. Individual missense variants were introduced using the QuikChange II XL Site-Directed Mutagenesis kit (RRID: SCR_020426 ), per the manufacturer's protocol (Agilent Technologies Inc., USA; primers listed in ).

Transfection:

Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis
Article Snippet: HEK293 were stably transfected with YFP (control) (addgene #84910), YFP tagged TDP-43 WT (addgene: #84911), or YFP tagged TDP-43 ΔNLS (addgene: #84912) plasmids and selected for at least 2 weeks using 200 μg/mL of G418 selection reagent (Enzo: #ALX-380-013). .. NSC-34 cells were transfected with flag (control) (addgene: #52535), flag tagged TDP-43 WT (addgene: #141327), or flag tagged TDP-43 A315T (addgene: #141329) plasmids and were selected for at least 2 weeks using (2 μg/mL) of puromycin. .. HEK293 cells were transiently co-overexpressed with either YFP (control)/TDP-43 WT /TDP-43 ΔNLS and Flag/Flag-tagged HK1 plasmids with TransIT-2020 according to manufacturer’s protocol.

Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis.
Article Snippet: HEK293 were stably transfected with YFP (control) (addgene #84910), YFP tagged TDP-43WT (addgene: #84911), or YFP tagged TDP-43ΔNLS (addgene: #84912) plasmids and selected for at least 2 weeks using 200 μg/mL of G418 selection reagent (Enzo: #ALX-380-013). .. NSC-34 cells were transfected with flag (control) (addgene: #52535), flag tagged TDP-43WT (addgene: #141327), or flag tagged TDP-43A315T (addgene: #141329) plasmids and were selected for at least 2 weeks using (2 μg/mL) of puromycin. .. HEK293 cells were transiently co-overexpressed with either YFP (control)/TDP-43WT/TDP-43ΔNLS and Flag/Flag-tagged HK1 plasmids with TransIT-2020 according to manufacturer’s protocol.

Control:

Clone Assay:

Article Title: Identification of TMEM263 as an ER-resident protein that controls early stages of lipid droplet biogenesis
Article Snippet: .. TMEM263cDNA (NP_001306590.1) was purchased from Integrated DNA Technologies (IDT) and cloned into pEGFP-C2 (Clontech), p-mCherry2-C1 (addgene #54563)), FLAGHA-pcDNA3.1 (addgene #52535). ..

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