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fcm fixation buffer  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology fcm fixation buffer
    Fcm Fixation Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixation+buffer/FCM+Fixation+Buffer/pmc02824744-261-31-34
    Average 93 stars, based on 24 article reviews
    fcm fixation buffer - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Single Cell Gel Electrophoresis:

    Article Title: Low-dose bisphenol A (BPA)-induced DNA damage and tumorigenic events in MCF-10A cells
    Article Snippet: Anti-BrdU mouse monoclonal antibody (Biolegend cat #339801) was diluted 1:250 in dilution buffer prepared in bovine serum albumin (BSA). .. Secondary antibody for comet assay was donkey anti-mouse Alexa 488 conjugate (Santa Cruz sc-362248) used at 1:5000 dilution. .. We used NF-κB sampler kit provided by Cell Signaling (cat # 9936) for primary antibodies.

    Incubation:

    Article Title: <b>Investigation of the Apoptotic Effects of Mesenchymal Stem Cells on KG-1 Leukemic Cell Line</b>
    Article Snippet: .. Then, the cells were fixed by incubating with FCM fixation buffer for 30 min (sc-3622, Santa Cruz, CA), followed by washing and permeabilizing with FCM permeabilization buffer (sc-3623, Santa Cruz, CA) with incubation of 5 min at RT. .. After washing cells, FITC Active Anti-caspase-3 conjugated (Cat No:550480, BD Bioscience, Belgium) was added to the cells for 30 minutes.

    Activity Assay:

    Article Title: Investigation of L-carnitine effects on CD44 + cancer stem cells from MDA-MB-231 breast cancer cell line as anti-cancer therapy
    Article Snippet: .. To evaluate caspase-3 enzymatic activity, harvested cells were washed twice with washing buffer (PBS containing 5% FBS) and immediately fixed by FCM fixation buffer for 30 min (sc-3622, Santa Cruz, CA) and washed twice with PBS buffer. .. The cells were permeabilized by incubating with FCM permeabilization buffer for 5 min at RT (sc-3623, Santa Cruz, CA).

    Staining:

    Article Title: MafK mediates chromatin remodeling to silence IRF8 expression in non-immune cells in a lineage-specific manner
    Article Snippet: Flow cytometry analysis was performed using BD LSR-II flow cytometer (BD Bioscience, San Jose, CA, USA) and data was analyzed using Flowing Software 2 (Cell Imaging Core, Turku Centre for Biotechnology). .. For IRF8 staining, cells were fixed in 4% paraformaldehyde, permeabilized with 0.5% saponin, blocked with 10% normal donkey serum (Sigma-Aldrich) and stained with either goat anti-IRF8 (Santa Cruz, CAT# sc-6058) and CFL405 anti-goat IgG (Santa Cruz, CAT# sc-362245) or with CFL405 anti-goat IgG alone as control. ..

    Control:

    Article Title: MafK mediates chromatin remodeling to silence IRF8 expression in non-immune cells in a lineage-specific manner
    Article Snippet: Flow cytometry analysis was performed using BD LSR-II flow cytometer (BD Bioscience, San Jose, CA, USA) and data was analyzed using Flowing Software 2 (Cell Imaging Core, Turku Centre for Biotechnology). .. For IRF8 staining, cells were fixed in 4% paraformaldehyde, permeabilized with 0.5% saponin, blocked with 10% normal donkey serum (Sigma-Aldrich) and stained with either goat anti-IRF8 (Santa Cruz, CAT# sc-6058) and CFL405 anti-goat IgG (Santa Cruz, CAT# sc-362245) or with CFL405 anti-goat IgG alone as control. ..



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