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r d for fgf21 df2100  (Cusabio)


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    Structured Review

    Cusabio r d for fgf21 df2100
    Mitokine assessment.
    R D For Fgf21 Df2100, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/FGF21/pmc07346035-178-16-23
    Average 93 stars, based on 34 article reviews
    r d for fgf21 df2100 - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Both objective and paradoxical insomnia elicit a stress response involving mitokine production"

    Article Title: Both objective and paradoxical insomnia elicit a stress response involving mitokine production

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.103274

    Mitokine assessment.
    Figure Legend Snippet: Mitokine assessment.

    Techniques Used:

    Related Articles

    Blocking Assay:

    Article Title: Establishment of a Direct Competitive ELISA for Camel FGF21 Detection
    Article Snippet: For the western blot test, gel after electrophoresis was electrotransferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Bedford, MA, USA). .. After blocking with 10% skimmed milk in TBST for 2 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies, including the anti-camel FGF21 polyclonal antibody mentioned above, anti-His tag antibody (CusaBio, Wuhan, China), or anti-human FGF21 antibody (ab171941, Abcam, UK). .. After three washes with TBST, the membranes were incubated with the corresponding HRP-coupled secondary antibody (CusaBio) for 1 h at 37 °C.

    Incubation:

    Article Title: Establishment of a Direct Competitive ELISA for Camel FGF21 Detection
    Article Snippet: For the western blot test, gel after electrophoresis was electrotransferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Bedford, MA, USA). .. After blocking with 10% skimmed milk in TBST for 2 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies, including the anti-camel FGF21 polyclonal antibody mentioned above, anti-His tag antibody (CusaBio, Wuhan, China), or anti-human FGF21 antibody (ab171941, Abcam, UK). .. After three washes with TBST, the membranes were incubated with the corresponding HRP-coupled secondary antibody (CusaBio) for 1 h at 37 °C.

    Clinical Proteomics:

    Article Title: Impaired hepatic BMAL1-FGF21 signaling drives adverse metabolic outcomes of ketogenic diet.
    Article Snippet: Aims The ketogenic diet (KD) has gained popularity for its metabolic benefits; however, its effects vary markedly across physiological and pathological conditions.. This study aimed to determine the mechanisms underlying differential metabolic responses to KD.. Materials and Methods Db/db, liver-specific fibroblast growth factor 21 (FGF21) knockdown mice, and liverspecific brain and muscle aryl hydrocarbon receptor nuclear translocator-like 1 (BMAL1) knockout mice were treated with isocaloric KD for 8 weeks.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Impaired hepatic BMAL1-FGF21 signaling drives adverse metabolic outcomes of ketogenic diet.
    Article Snippet: Aims The ketogenic diet (KD) has gained popularity for its metabolic benefits; however, its effects vary markedly across physiological and pathological conditions.. This study aimed to determine the mechanisms underlying differential metabolic responses to KD.. Materials and Methods Db/db, liver-specific fibroblast growth factor 21 (FGF21) knockdown mice, and liverspecific brain and muscle aryl hydrocarbon receptor nuclear translocator-like 1 (BMAL1) knockout mice were treated with isocaloric KD for 8 weeks.

    Recombinant:

    Article Title: Ultrasound-targeted microbubble destruction technology delivering β-klotho to the heart enhances FGF21 sensitivity and attenuates heart remodeling post-myocardial infarction
    Article Snippet: The rats were administered an intraperitoneal injection of the vehicle (0.9% saline, ST341, Beyotime Institute of Biotechnology) or FGF21 at 0.5 mg/kg/day. .. Recombinant human FGF21 (CSB-AP002471HU, Cusabio Technology, LLC) was expressed in E. coli and harvested by ion exchange and hydrophobic interaction chromatography, as previously described ( ). ..

    Hydrophobic Interaction Chromatography:

    Article Title: Ultrasound-targeted microbubble destruction technology delivering β-klotho to the heart enhances FGF21 sensitivity and attenuates heart remodeling post-myocardial infarction
    Article Snippet: The rats were administered an intraperitoneal injection of the vehicle (0.9% saline, ST341, Beyotime Institute of Biotechnology) or FGF21 at 0.5 mg/kg/day. .. Recombinant human FGF21 (CSB-AP002471HU, Cusabio Technology, LLC) was expressed in E. coli and harvested by ion exchange and hydrophobic interaction chromatography, as previously described ( ). ..

    other:

    Article Title: Establishment of a Direct Competitive ELISA for Camel FGF21 Detection.
    Article Snippet: After blocking with 10% skimmed milk in TBST for 2 h at room temperature, the membranes were incubated overnight at 4 ◦C with primary antibodies, including the anti-camel FGF21 polyclonal antibody mentioned above, anti-His tag antibody (CusaBio, Wuhan, China), or anti-human FGF21 antibody (ab171941, Abcam, Vet.



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    Image Search Results


    Circulating biomarkers levels in chronic heart failure patients and controls

    Journal: ESC Heart Failure

    Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

    doi: 10.1093/eschf/xvag229

    Figure Lengend Snippet: Circulating biomarkers levels in chronic heart failure patients and controls

    Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

    Techniques:

    Circulating miRNAs and cytokines as molecular signatures of heart failure. Differences in serum levels of GDF-15 and FGF-21 in CHF patients and controls. Serum levels of GDF-15 by ELISA assay in patients with heart failure (HF) ( n = 25) and the control group ( n = 10). ( A ) Data showed significant upregulation of GDF-15 in CHF vs controls ( P < .001). ( B ) FGF-21 levels did not differ significantly between groups. Correlations between GDF-15 and selected miRNAs in CHF patients. ( C , D ) In CHF patients, GDF-15 levels were positively associated with miR-29b ( ρ = 0.42, P = .037) and miR-486 ( ρ = 0.42, P = .039). Data are expressed as fold change and are presented as mean ± SEM. Student's t -test or Mann–Whitney test was used for normally distributed data or non-normally distributed data, respectively. *** P ≤ .001.

    Journal: ESC Heart Failure

    Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

    doi: 10.1093/eschf/xvag229

    Figure Lengend Snippet: Circulating miRNAs and cytokines as molecular signatures of heart failure. Differences in serum levels of GDF-15 and FGF-21 in CHF patients and controls. Serum levels of GDF-15 by ELISA assay in patients with heart failure (HF) ( n = 25) and the control group ( n = 10). ( A ) Data showed significant upregulation of GDF-15 in CHF vs controls ( P < .001). ( B ) FGF-21 levels did not differ significantly between groups. Correlations between GDF-15 and selected miRNAs in CHF patients. ( C , D ) In CHF patients, GDF-15 levels were positively associated with miR-29b ( ρ = 0.42, P = .037) and miR-486 ( ρ = 0.42, P = .039). Data are expressed as fold change and are presented as mean ± SEM. Student's t -test or Mann–Whitney test was used for normally distributed data or non-normally distributed data, respectively. *** P ≤ .001.

    Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Control, MANN-WHITNEY

    Molecular profiles in CHF patients. Differences in serum levels of GDF-15 and FGF-21 among heart failure patients with and without cardiac cachexia. Circulating levels of GDF-15 and FGF-21 were analyzed in CHF patients with cardiac cachexia (CC) ( n = 10) and without cardiac cachexia (nCC) ( n = 15), and a control group ( n = 10). ( A ) Significant difference in GDF-15 levels was observed between patients with CC or nCC vs controls (all P < .001). ( B ) Levels of FGF-21 were significantly higher in CHF with CC compared with nCC ( P = .012) and vs controls ( P = .010) ( C ). Spearman’s correlation analysis revealed that in CHF patients with CC, GDF-15 negatively correlated with miR-21 ( ρ = −0.81, P = .007). ( D ) In the same group, FGF-21 negatively correlated with miR-486 ( ρ = −0.69, P = .035). Data are expressed as fold change and are presented as mean ± SEM. One-way ANOVA test or Kruskal–Wallis test was used for normally distributed data or non-normally distributed data, respectively. * P < .05, *** P ≤ .001.

    Journal: ESC Heart Failure

    Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

    doi: 10.1093/eschf/xvag229

    Figure Lengend Snippet: Molecular profiles in CHF patients. Differences in serum levels of GDF-15 and FGF-21 among heart failure patients with and without cardiac cachexia. Circulating levels of GDF-15 and FGF-21 were analyzed in CHF patients with cardiac cachexia (CC) ( n = 10) and without cardiac cachexia (nCC) ( n = 15), and a control group ( n = 10). ( A ) Significant difference in GDF-15 levels was observed between patients with CC or nCC vs controls (all P < .001). ( B ) Levels of FGF-21 were significantly higher in CHF with CC compared with nCC ( P = .012) and vs controls ( P = .010) ( C ). Spearman’s correlation analysis revealed that in CHF patients with CC, GDF-15 negatively correlated with miR-21 ( ρ = −0.81, P = .007). ( D ) In the same group, FGF-21 negatively correlated with miR-486 ( ρ = −0.69, P = .035). Data are expressed as fold change and are presented as mean ± SEM. One-way ANOVA test or Kruskal–Wallis test was used for normally distributed data or non-normally distributed data, respectively. * P < .05, *** P ≤ .001.

    Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

    Techniques: Control, Analysis

    Molecular profiles in cachectic patients with severe CHF. FGF-21 concentrations and their associations with NYHA functional class in patients with CHF. ( A ) Data show higher FGF-21 levels in NYHA class III-IV ( n = 13) compared with class I-II ( n = 9) ( P = .050) and the control group ( n = 10) ( P = .050). ( B ) In NYHA class III-IV, FGF-21 positively correlated with miR-15b ( r = 0.68, P = .014). Data are expressed as fold change and are presented as mean ± SEM. One-way ANOVA test or Kruskal–Wallis test was used for normally distributed data or non-normally distributed data, respectively. * P < .05.

    Journal: ESC Heart Failure

    Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

    doi: 10.1093/eschf/xvag229

    Figure Lengend Snippet: Molecular profiles in cachectic patients with severe CHF. FGF-21 concentrations and their associations with NYHA functional class in patients with CHF. ( A ) Data show higher FGF-21 levels in NYHA class III-IV ( n = 13) compared with class I-II ( n = 9) ( P = .050) and the control group ( n = 10) ( P = .050). ( B ) In NYHA class III-IV, FGF-21 positively correlated with miR-15b ( r = 0.68, P = .014). Data are expressed as fold change and are presented as mean ± SEM. One-way ANOVA test or Kruskal–Wallis test was used for normally distributed data or non-normally distributed data, respectively. * P < .05.

    Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

    Techniques: Functional Assay, Control

    Schematic representation of the circulating molecular profile associated with chronic heart failure and cardiac cachexia, characterized by dysregulation of muscle- and remodelling-related miRNAs and increased stress-responsive cytokines. CHF patients showed reduced miR-15b, miR-29b, miR-133a, and miR-486 and increased GDF-15, while cardiac cachexia was further associated with lower miR-29b and higher FGF-21 levels.

    Journal: ESC Heart Failure

    Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

    doi: 10.1093/eschf/xvag229

    Figure Lengend Snippet: Schematic representation of the circulating molecular profile associated with chronic heart failure and cardiac cachexia, characterized by dysregulation of muscle- and remodelling-related miRNAs and increased stress-responsive cytokines. CHF patients showed reduced miR-15b, miR-29b, miR-133a, and miR-486 and increased GDF-15, while cardiac cachexia was further associated with lower miR-29b and higher FGF-21 levels.

    Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

    Techniques: