fixation buffer (Becton Dickinson)

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Becton Dickinson fixation buffer
Fixation Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fcm+tm+fixation+buffer/fixation+buffer/pmc09369201-160-5-8
Average 90 stars, based on 1 article reviews

fixation buffer - by Bioz Stars, 2026-11

90/100 stars

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other:

Article Title: The CD33xCD123xCD70 Multispecific CD3-Engaging DARPin MP0533 Induces Selective T Cell–Mediated Killing of AML Leukemic Stem Cells
Article Snippet: In a next step, cells were washed with 100 μL FACS buffer and stained for surface markers by using directly labeled antibodies against human CD4 (OKT4, 1:200), CD8a (RPA-T8, 1:100), CD25 (BC96, 1:200), CD69 (FN50, 1:100), PD-1 (EH12.2H7, 1:100), LAG-3 (7H2C65, 1:200), and TIM-3 (F38-2E2, 1:200; all from BioLegend) in 100 μL FACS buffer for 30 minutes at 4°C in the dark.

Article Title: The CD33xCD123xCD70 Multispecific CD3-Engaging DARPin MP0533 Induces Selective T Cell–Mediated Killing of AML Leukemic Stem Cells
Article Snippet: Expression of CD33, CD123, and CD70 was analyzed on HUVEC after 24 hours culture under unstimulated, low (0.3 ng/mL TNFα and 1 ng/mL IFNɣ), or high (10 ng/mL TNFα and 10 ng/mL IFNɣ) cytokine (Peprotech) treatment conditions.

Incubation:

Article Title: Maternal humoral factors modulate offspring gut immune homeostasis to mitigate diabetes development
Article Snippet: .. Samples were incubated at 4°C for 30 min, washed, centrifuged, and resuspended in Fixation buffer, then analyzed by flow cytometry on a BD LSRFortessa X-20. .. FlowJo (BD, v10.8) was used to analyze all cytometry data.

Article Title: Generation of a human SLC12A3 knock-in human induced pluripotent stem cell line (CMCi014-A-82) using CRISPR-Cas9 system.
Article Snippet: After selection, genomic PCR was performed using AccuPower® PCR PreMix & Master Mix (Bioneer) to identify the selected WTC11-SLC12A3 clonies with the primer sequences in Table 2. .. The suspended single cell was incubated in Fixation buffer (BD) at 4◦C for 30 min in the dark, then permeabilizated with the Perm/Wash buffer (BD) at 4◦C for 20 min. .. The cells were incubated with the primary antibodies, then read the fluorescence using BD CantoII and analyzed using the FlowJo V10 Single Cell Analysis Software (TreeStar).

Article Title: METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation
Article Snippet: .. Fixation buffer (BD Biosciences) was used to fix these cells at room temperature for 30 min. After washed once with PBS, these cells were permeated in perm/wash buffer (BD Biosciences) at 4 °C for 15 min. After that, these cells were incubated with corresponding primary antibodies at 37 °C for 30 min. After washed once with PBS, these cells were incubated with corresponding secondary antibodies at 37 °C for 30 min. ..

Article Title: A Genome-Wide CRISPR Screen Identifies BRD4 as a Regulator of Cardiomyocyte Differentiation
Article Snippet: Antibodies used were mouse anti-FLAG (Sigma-Aldrich, F1804), rabbit anti-BRD4 (EpiCypher, 13–2003), rabbit anti-H3K4Me3 (EpiCypher, 13–0041), rabbit anti-H3K27Me3 (EpiCypher, 13–0055) and rabbit IgG (EpiCypher, 13–0042). .. Approximately 1 × 10 6 cells were fixed using fixation buffer (BD Biosciences, 554655), washed two times with 1x permeabilization/wash buffer (BD Biosciences, 554723), incubated for 15 min in 1x permeabilization/wash buffer and resuspended in 100 μl of 1x permeabilization/wash buffer supplemented with 1 μl of the appropriate antibody at room temperature for 40 min with gentle rocking. ..

Flow Cytometry:

Single Cell:

Gentle:

Staining:

Article Title: HP inulin-MCT dietary fiber improves lipid metabolism and prevents non-alcoholic steatohepatitis in obese mice
Article Snippet: .. For intracellular staining, the splenocytes were resuspended in 1 ml permeabilization buffer (Thermo Fisher Scientific Inc., Massachusetts, USA) for 10 min. After washings, splenocytes were stained with 10μg APC-labeled anti-Foxp3, washed and fixed with fixation buffer (BD Cytofix). .. T-cells were analyzed by flow cytometry (FACSCalibur; Becton Dickinson, CA) using flowing software 2TM (Turku Bioscience, Finland).

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