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Becton Dickinson fc receptor blocking solution
Fc Receptor Blocking Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fc+blocking+solution/fc+receptor+blocking+solution/pmc07115095-40-7-12
Average 90 stars, based on 1 article reviews
fc receptor blocking solution - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration
Article Snippet: Cells were washed twice in PBS and stained with 1 μg/mL DAPI (D9542, Sigma-Aldrich) for 15 min at room temperature (RT) in the dark. .. After two successive washes with a staining buffer (PBS, 1% bovine serum albumin), cells were incubated with a Fc receptor blocking solution (564219, BD Pharmingen) for 15 min at RT in the dark. .. Subsequently, cells were stained with Alexa Fluor® 488-conjugated anti-αVβ3 antibody (Novus Biologicals, USA) (39.5 μg/mL) and Alexa Fluor® 700-conjugated anti-αVβ6 antibody (Novus Biologicals) (30.5 μg/mL), or with isotype controls (Alexa Fluor® 488-conjugated mouse IgG1 (R&D systems, USA) and Alexa Fluor® 700-conjugated rat IgG2 (Novus Biologicals) for 20 min at 4°C in the dark.

Article Title: An Fc-Competent Anti-Human TIGIT Blocking Antibody Ociperlimab (BGB-A1217) Elicits Strong Immune Responses and Potent Anti-Tumor Efficacy in Pre-Clinical Models
Article Snippet: .. Prior to antibody labeling, cell suspensions were preincubated with Live/Dead fixable Dead Cell Stain Kit (Invitrogen, catalog no. 65-0866-14) at room temperature for 20 minutes, Fc receptor blocking solution (BD, catalog no. 553141) at 4°C for 10 minutes, and 10μg/mL BGB-A1217 at 4°C for 30 minutes. ..

Article Title: An Fc-Competent Anti-Human TIGIT Blocking Antibody Ociperlimab (BGB-A1217) Elicits Strong Immune Responses and Potent Anti-Tumor Efficacy in Pre-Clinical Models.
Article Snippet: .. Prior to antibody labeling, cell suspensions were preincubated with Live/ Dead fixable Dead Cell Stain Kit (Invitrogen, catalog no. 65-0866- 14) at room temperature for 20 minutes, Fc receptor blocking solution (BD, catalog no. 553141) at 4°C for 10 minutes, and 10mg/ mL BGB-A1217 at 4°C for 30 minutes. ..

Article Title: Characterization of pathological stages in a mouse model of progressive multiple sclerosis.
Article Snippet: .. The cells were stained with Fixable Viability Dye eFluor780 (FVD780, 1:1000, eBioscience, Inc.) to discriminate between live and dead cells, incubated with Fc receptor blocking solution (1:200, BD Biosciences) to prevent nonspecific antibody binding, and incubated with antibody solution diluted in Stain Buffer (BD Biosciences) on ice for 20 min. .. The following fluorescently labeled antibodies were used: BUV395-CD45, BUV-737 B220, BV421-CD3e, BV605-CD4, FITC-PD-1, APC-CXCR5, AF700-CD8a (all from BioLegend, Inc.).

Article Title: The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration
Article Snippet: Cells were washed twice in PBS and stained with 1 μg/mL DAPI (D9542, Sigma-Aldrich) for 15 min at room temperature (RT) in the dark. .. After two successive washes with a staining buffer (PBS, 1% bovine serum albumin), cells were incubated with a Fc receptor blocking solution (564219, BD Pharmingen) for 15 min at RT in the dark. .. Subsequently, cells were stained with Alexa Fluor® 488-conjugated anti-αVβ3 antibody (Novus Biologicals, USA, FAB3050G) (39.5 μg/mL) and Alexa Fluor® 700-conjugated anti-αVβ6 antibody (Novus Biologicals, NBP2-50448AF700) (30.5 μg/mL), or with isotype controls (Alexa Fluor® 488-conjugated mouse IgG1 (R&D systems, USA, FAB110G) and Alexa Fluor® 700-conjugated rat IgG2 (Novus Biologicals, IC006N) for 20 min at 4 °C in the dark.

Incubation:

Article Title: The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration
Article Snippet: Cells were washed twice in PBS and stained with 1 μg/mL DAPI (D9542, Sigma-Aldrich) for 15 min at room temperature (RT) in the dark. .. After two successive washes with a staining buffer (PBS, 1% bovine serum albumin), cells were incubated with a Fc receptor blocking solution (564219, BD Pharmingen) for 15 min at RT in the dark. .. Subsequently, cells were stained with Alexa Fluor® 488-conjugated anti-αVβ3 antibody (Novus Biologicals, USA) (39.5 μg/mL) and Alexa Fluor® 700-conjugated anti-αVβ6 antibody (Novus Biologicals) (30.5 μg/mL), or with isotype controls (Alexa Fluor® 488-conjugated mouse IgG1 (R&D systems, USA) and Alexa Fluor® 700-conjugated rat IgG2 (Novus Biologicals) for 20 min at 4°C in the dark.

Article Title: Characterization of pathological stages in a mouse model of progressive multiple sclerosis.
Article Snippet: .. The cells were stained with Fixable Viability Dye eFluor780 (FVD780, 1:1000, eBioscience, Inc.) to discriminate between live and dead cells, incubated with Fc receptor blocking solution (1:200, BD Biosciences) to prevent nonspecific antibody binding, and incubated with antibody solution diluted in Stain Buffer (BD Biosciences) on ice for 20 min. .. The following fluorescently labeled antibodies were used: BUV395-CD45, BUV-737 B220, BV421-CD3e, BV605-CD4, FITC-PD-1, APC-CXCR5, AF700-CD8a (all from BioLegend, Inc.).

Article Title: The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration
Article Snippet: Cells were washed twice in PBS and stained with 1 μg/mL DAPI (D9542, Sigma-Aldrich) for 15 min at room temperature (RT) in the dark. .. After two successive washes with a staining buffer (PBS, 1% bovine serum albumin), cells were incubated with a Fc receptor blocking solution (564219, BD Pharmingen) for 15 min at RT in the dark. .. Subsequently, cells were stained with Alexa Fluor® 488-conjugated anti-αVβ3 antibody (Novus Biologicals, USA, FAB3050G) (39.5 μg/mL) and Alexa Fluor® 700-conjugated anti-αVβ6 antibody (Novus Biologicals, NBP2-50448AF700) (30.5 μg/mL), or with isotype controls (Alexa Fluor® 488-conjugated mouse IgG1 (R&D systems, USA, FAB110G) and Alexa Fluor® 700-conjugated rat IgG2 (Novus Biologicals, IC006N) for 20 min at 4 °C in the dark.

Article Title: Compositions, combinations and related methods for photoimmunotherapy
Article Snippet: .. The cells were divided into three groups (all in triplicate) as follows: (1) THP1 cells only (untreated); (2) THP1 cells treated with the drug cetuximab-IRDye 700DX at 500 ng/mL; and (3) THP1 cells first incubated with Fc receptor blocking solution (Catalog No: 564220, BD, Franklin Lakes, N.J.) at 1 μg/well for 20 min at room temperature followed by treatment with drug cetuximab-IRDye 700DX (500 ng/mL, 1 hr at 37° C. in incubator protected from light). ..

Article Title: Compositions, combinations and related methods for photoimmunotherapy
Article Snippet: .. The cells were divided into three groups (all in triplicate) as follows: (1) THP1 cells only (untreated); (2) THP1 cells treated with the drug cetuximab-IRDye 700DX at 500 ng/mL; and (3) THP1 cells first incubated with Fc receptor blocking solution (Catalog No: 564220, BD, Franklin Lakes, N.J.) at 1 μg/well for 20 min at room temperature followed by treatment with drug cetuximab-IRDye 700DX (500 ng/mL, 1 hr at 37° C. in incubator protected from light). ..

Article Title: Transcriptomic profiling of microglia and astrocytes throughout aging
Article Snippet: .. After isolation, cell pellets were resuspended in FC receptor blocking solution (553141, BD Biosciences), incubated on ice for 10 min, and costained for 30 min on ice in the dark with PE-Cy7-labeled CD45 (103114, BioLegend), PE-labeled CD11b (101208, BioLegend), and APC-labeled ACSA2 (130-117-386, Miltenyi Biotec). .. The cells were then rinsed in PBS, centrifuged, resuspended in FACS buffer (1% FBS + 2 mM EDTA, 25 mM HEPES, 1:500 RNA inhibitor in PBS), and incubated in 7AAD (420403, BioLegend) for 10 min before sorting.

Blocking Assay:

Article Title: The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration
Article Snippet: Cells were washed twice in PBS and stained with 1 μg/mL DAPI (D9542, Sigma-Aldrich) for 15 min at room temperature (RT) in the dark. .. After two successive washes with a staining buffer (PBS, 1% bovine serum albumin), cells were incubated with a Fc receptor blocking solution (564219, BD Pharmingen) for 15 min at RT in the dark. .. Subsequently, cells were stained with Alexa Fluor® 488-conjugated anti-αVβ3 antibody (Novus Biologicals, USA) (39.5 μg/mL) and Alexa Fluor® 700-conjugated anti-αVβ6 antibody (Novus Biologicals) (30.5 μg/mL), or with isotype controls (Alexa Fluor® 488-conjugated mouse IgG1 (R&D systems, USA) and Alexa Fluor® 700-conjugated rat IgG2 (Novus Biologicals) for 20 min at 4°C in the dark.

Article Title: An Fc-Competent Anti-Human TIGIT Blocking Antibody Ociperlimab (BGB-A1217) Elicits Strong Immune Responses and Potent Anti-Tumor Efficacy in Pre-Clinical Models
Article Snippet: .. Prior to antibody labeling, cell suspensions were preincubated with Live/Dead fixable Dead Cell Stain Kit (Invitrogen, catalog no. 65-0866-14) at room temperature for 20 minutes, Fc receptor blocking solution (BD, catalog no. 553141) at 4°C for 10 minutes, and 10μg/mL BGB-A1217 at 4°C for 30 minutes. ..

Article Title: An Fc-Competent Anti-Human TIGIT Blocking Antibody Ociperlimab (BGB-A1217) Elicits Strong Immune Responses and Potent Anti-Tumor Efficacy in Pre-Clinical Models.
Article Snippet: .. Prior to antibody labeling, cell suspensions were preincubated with Live/ Dead fixable Dead Cell Stain Kit (Invitrogen, catalog no. 65-0866- 14) at room temperature for 20 minutes, Fc receptor blocking solution (BD, catalog no. 553141) at 4°C for 10 minutes, and 10mg/ mL BGB-A1217 at 4°C for 30 minutes. ..

Article Title: Characterization of pathological stages in a mouse model of progressive multiple sclerosis.
Article Snippet: .. The cells were stained with Fixable Viability Dye eFluor780 (FVD780, 1:1000, eBioscience, Inc.) to discriminate between live and dead cells, incubated with Fc receptor blocking solution (1:200, BD Biosciences) to prevent nonspecific antibody binding, and incubated with antibody solution diluted in Stain Buffer (BD Biosciences) on ice for 20 min. .. The following fluorescently labeled antibodies were used: BUV395-CD45, BUV-737 B220, BV421-CD3e, BV605-CD4, FITC-PD-1, APC-CXCR5, AF700-CD8a (all from BioLegend, Inc.).

Article Title: The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration
Article Snippet: Cells were washed twice in PBS and stained with 1 μg/mL DAPI (D9542, Sigma-Aldrich) for 15 min at room temperature (RT) in the dark. .. After two successive washes with a staining buffer (PBS, 1% bovine serum albumin), cells were incubated with a Fc receptor blocking solution (564219, BD Pharmingen) for 15 min at RT in the dark. .. Subsequently, cells were stained with Alexa Fluor® 488-conjugated anti-αVβ3 antibody (Novus Biologicals, USA, FAB3050G) (39.5 μg/mL) and Alexa Fluor® 700-conjugated anti-αVβ6 antibody (Novus Biologicals, NBP2-50448AF700) (30.5 μg/mL), or with isotype controls (Alexa Fluor® 488-conjugated mouse IgG1 (R&D systems, USA, FAB110G) and Alexa Fluor® 700-conjugated rat IgG2 (Novus Biologicals, IC006N) for 20 min at 4 °C in the dark.

Article Title: Compositions, combinations and related methods for photoimmunotherapy
Article Snippet: .. The cells were divided into three groups (all in triplicate) as follows: (1) THP1 cells only (untreated); (2) THP1 cells treated with the drug cetuximab-IRDye 700DX at 500 ng/mL; and (3) THP1 cells first incubated with Fc receptor blocking solution (Catalog No: 564220, BD, Franklin Lakes, N.J.) at 1 μg/well for 20 min at room temperature followed by treatment with drug cetuximab-IRDye 700DX (500 ng/mL, 1 hr at 37° C. in incubator protected from light). ..

Article Title: Compositions, combinations and related methods for photoimmunotherapy
Article Snippet: .. The cells were divided into three groups (all in triplicate) as follows: (1) THP1 cells only (untreated); (2) THP1 cells treated with the drug cetuximab-IRDye 700DX at 500 ng/mL; and (3) THP1 cells first incubated with Fc receptor blocking solution (Catalog No: 564220, BD, Franklin Lakes, N.J.) at 1 μg/well for 20 min at room temperature followed by treatment with drug cetuximab-IRDye 700DX (500 ng/mL, 1 hr at 37° C. in incubator protected from light). ..

Article Title: Transcriptomic profiling of microglia and astrocytes throughout aging
Article Snippet: .. After isolation, cell pellets were resuspended in FC receptor blocking solution (553141, BD Biosciences), incubated on ice for 10 min, and costained for 30 min on ice in the dark with PE-Cy7-labeled CD45 (103114, BioLegend), PE-labeled CD11b (101208, BioLegend), and APC-labeled ACSA2 (130-117-386, Miltenyi Biotec). .. The cells were then rinsed in PBS, centrifuged, resuspended in FACS buffer (1% FBS + 2 mM EDTA, 25 mM HEPES, 1:500 RNA inhibitor in PBS), and incubated in 7AAD (420403, BioLegend) for 10 min before sorting.

Antibody Labeling:

Article Title: An Fc-Competent Anti-Human TIGIT Blocking Antibody Ociperlimab (BGB-A1217) Elicits Strong Immune Responses and Potent Anti-Tumor Efficacy in Pre-Clinical Models
Article Snippet: .. Prior to antibody labeling, cell suspensions were preincubated with Live/Dead fixable Dead Cell Stain Kit (Invitrogen, catalog no. 65-0866-14) at room temperature for 20 minutes, Fc receptor blocking solution (BD, catalog no. 553141) at 4°C for 10 minutes, and 10μg/mL BGB-A1217 at 4°C for 30 minutes. ..

Article Title: An Fc-Competent Anti-Human TIGIT Blocking Antibody Ociperlimab (BGB-A1217) Elicits Strong Immune Responses and Potent Anti-Tumor Efficacy in Pre-Clinical Models.
Article Snippet: .. Prior to antibody labeling, cell suspensions were preincubated with Live/ Dead fixable Dead Cell Stain Kit (Invitrogen, catalog no. 65-0866- 14) at room temperature for 20 minutes, Fc receptor blocking solution (BD, catalog no. 553141) at 4°C for 10 minutes, and 10mg/ mL BGB-A1217 at 4°C for 30 minutes. ..

Binding Assay:

Article Title: Characterization of pathological stages in a mouse model of progressive multiple sclerosis.
Article Snippet: .. The cells were stained with Fixable Viability Dye eFluor780 (FVD780, 1:1000, eBioscience, Inc.) to discriminate between live and dead cells, incubated with Fc receptor blocking solution (1:200, BD Biosciences) to prevent nonspecific antibody binding, and incubated with antibody solution diluted in Stain Buffer (BD Biosciences) on ice for 20 min. .. The following fluorescently labeled antibodies were used: BUV395-CD45, BUV-737 B220, BV421-CD3e, BV605-CD4, FITC-PD-1, APC-CXCR5, AF700-CD8a (all from BioLegend, Inc.).

Isolation:

Article Title: Transcriptomic profiling of microglia and astrocytes throughout aging
Article Snippet: .. After isolation, cell pellets were resuspended in FC receptor blocking solution (553141, BD Biosciences), incubated on ice for 10 min, and costained for 30 min on ice in the dark with PE-Cy7-labeled CD45 (103114, BioLegend), PE-labeled CD11b (101208, BioLegend), and APC-labeled ACSA2 (130-117-386, Miltenyi Biotec). .. The cells were then rinsed in PBS, centrifuged, resuspended in FACS buffer (1% FBS + 2 mM EDTA, 25 mM HEPES, 1:500 RNA inhibitor in PBS), and incubated in 7AAD (420403, BioLegend) for 10 min before sorting.



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