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rneasybacteria protect mini kit  (Qiagen)


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    Structured Review

    Qiagen rneasybacteria protect mini kit
    Rneasybacteria Protect Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 2208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fairplay+microarray+labelling+kit/RNeasy+Protect+Mini+Kit/pmc04417155-172-5-9
    Average 97 stars, based on 2208 article reviews
    rneasybacteria protect mini kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A gain-of-function screening reveals dAnkmy2 as a potential mediator of lifespan extension and oxidative stress resistance in Drosophila melanogaster.
    Article Snippet: lifespans across genotypes and did not reveal robust lifespan-extending candidates.. In contrast, screening at 25 °C, where moderate hs-GAL4 induction produces robust and detectable transgene expression, revealed multiple longevity-promoting lines.. Lifespan measurements at the two temperatures were poorly correlated, indicating that elevated temperature imposes a dominant physiological burden that masks gene-specific effects.

    Synthesized:

    Article Title: A gain-of-function screening reveals dAnkmy2 as a potential mediator of lifespan extension and oxidative stress resistance in Drosophila melanogaster.
    Article Snippet: lifespans across genotypes and did not reveal robust lifespan-extending candidates.. In contrast, screening at 25 °C, where moderate hs-GAL4 induction produces robust and detectable transgene expression, revealed multiple longevity-promoting lines.. Lifespan measurements at the two temperatures were poorly correlated, indicating that elevated temperature imposes a dominant physiological burden that masks gene-specific effects.

    Article Title: Identification of pathological CD133+ endothelial cells in venous malformations
    Article Snippet: .. RNA was isolated using the RNeasy Mini Kit (Qiagen 74124). cDNA was synthesized using the SuperScript First-Strand Synthesis System (Invitrogen 11904018) ( ). .. Quantitative RT-PCR using gene specific primers ( ) and Sybr Green Master Mix (Thermofisher) was performed on a CFX96 PCR Cycler (Bio-Rad).

    Isolation:

    Article Title: Identification of pathological CD133+ endothelial cells in venous malformations
    Article Snippet: .. RNA was isolated using the RNeasy Mini Kit (Qiagen 74124). cDNA was synthesized using the SuperScript First-Strand Synthesis System (Invitrogen 11904018) ( ). .. Quantitative RT-PCR using gene specific primers ( ) and Sybr Green Master Mix (Thermofisher) was performed on a CFX96 PCR Cycler (Bio-Rad).

    Article Title: Transient analysis and engineering of bacterial metabolic pathways using cell-penetrating peptide-peptide nucleic acid conjugates.
    Article Snippet: .. Total RNA was then isolated and purified using the RNeasy Protect Mini Kit (QIAGEN K. K., Tokyo, Japan). .. Genomic DNA was removed by on-column DNase digestion using the QIAGEN RNase-Free DNase Set.

    Real-time Polymerase Chain Reaction:

    Article Title: FRZB-induced anti-angiogenic effect via Caveolin-1-mediated TGFβ signalling.
    Article Snippet: .. Quantitative Real-time PCR (qRT-PCR) RNA was extracted using an RNA extraction kit (QIAGEN, RNeasy Mini Kit) and was reverse transcribed into cDNA using qScript cDNA Synthesis Kit (Quantabio). .. PCR was conducted with QuantiTect Power SYBR Green (Applied Biosystems).

    RNA Extraction:

    Article Title: FRZB-induced anti-angiogenic effect via Caveolin-1-mediated TGFβ signalling.
    Article Snippet: .. Quantitative Real-time PCR (qRT-PCR) RNA was extracted using an RNA extraction kit (QIAGEN, RNeasy Mini Kit) and was reverse transcribed into cDNA using qScript cDNA Synthesis Kit (Quantabio). .. PCR was conducted with QuantiTect Power SYBR Green (Applied Biosystems).

    Article Title: Mitorubin, berberrubine-based compounds that improve mitochondrial function, exhibit cardioprotective effects against age-related cardiac dysfunction.
    Article Snippet: .. The 590/530 nm fluorescence ratio was calculated and used as an index of mitochondrial membrane potential. qRT-PCR analysis of C2C12 cells Total RNA was extracted from C2C12 cells using an RNA extraction kit (RNeasy Mini Kit, Qiagen). ..

    Reverse Transcription:

    Article Title: FRZB-induced anti-angiogenic effect via Caveolin-1-mediated TGFβ signalling.
    Article Snippet: .. Quantitative Real-time PCR (qRT-PCR) RNA was extracted using an RNA extraction kit (QIAGEN, RNeasy Mini Kit) and was reverse transcribed into cDNA using qScript cDNA Synthesis Kit (Quantabio). .. PCR was conducted with QuantiTect Power SYBR Green (Applied Biosystems).

    cDNA Synthesis:

    Article Title: FRZB-induced anti-angiogenic effect via Caveolin-1-mediated TGFβ signalling.
    Article Snippet: .. Quantitative Real-time PCR (qRT-PCR) RNA was extracted using an RNA extraction kit (QIAGEN, RNeasy Mini Kit) and was reverse transcribed into cDNA using qScript cDNA Synthesis Kit (Quantabio). .. PCR was conducted with QuantiTect Power SYBR Green (Applied Biosystems).

    Purification:

    Article Title: Transient analysis and engineering of bacterial metabolic pathways using cell-penetrating peptide-peptide nucleic acid conjugates.
    Article Snippet: .. Total RNA was then isolated and purified using the RNeasy Protect Mini Kit (QIAGEN K. K., Tokyo, Japan). .. Genomic DNA was removed by on-column DNase digestion using the QIAGEN RNase-Free DNase Set.

    Article Title: Generation of a HiBiT-expressing recombinant rat hepacivirus supporting both in vivo and in vitro infection
    Article Snippet: .. The NRHV1 NS3-specific primer and probe sequences used were as follows: The total RNA from tissue was purified with an RNeasy Protect Mini Kit (QIAGEN) and subjected to qRT–PCR as described for the infected cell samples. .. The HiBiT assay samples from organs were homogenized using a Handy Sonic in lysis buffer (100 mM Tris-HCl, 2 mM EDTA, 0.1% Triton X-100), and the supernatants obtained after centrifugation at 14000 × g for 30 min at 4°C were measured using a BCA protein kit (Nacalai Tesque) to determine the protein concentration, after which 10 μg of protein from the supernatants was used for the HiBiT assay.

    Fluorescence:

    Article Title: Mitorubin, berberrubine-based compounds that improve mitochondrial function, exhibit cardioprotective effects against age-related cardiac dysfunction.
    Article Snippet: .. The 590/530 nm fluorescence ratio was calculated and used as an index of mitochondrial membrane potential. qRT-PCR analysis of C2C12 cells Total RNA was extracted from C2C12 cells using an RNA extraction kit (RNeasy Mini Kit, Qiagen). ..

    Membrane:

    Article Title: Mitorubin, berberrubine-based compounds that improve mitochondrial function, exhibit cardioprotective effects against age-related cardiac dysfunction.
    Article Snippet: .. The 590/530 nm fluorescence ratio was calculated and used as an index of mitochondrial membrane potential. qRT-PCR analysis of C2C12 cells Total RNA was extracted from C2C12 cells using an RNA extraction kit (RNeasy Mini Kit, Qiagen). ..

    Quantitative RT-PCR:

    Article Title: Mitorubin, berberrubine-based compounds that improve mitochondrial function, exhibit cardioprotective effects against age-related cardiac dysfunction.
    Article Snippet: .. The 590/530 nm fluorescence ratio was calculated and used as an index of mitochondrial membrane potential. qRT-PCR analysis of C2C12 cells Total RNA was extracted from C2C12 cells using an RNA extraction kit (RNeasy Mini Kit, Qiagen). ..

    Infection:

    Article Title: Generation of a HiBiT-expressing recombinant rat hepacivirus supporting both in vivo and in vitro infection
    Article Snippet: .. The NRHV1 NS3-specific primer and probe sequences used were as follows: The total RNA from tissue was purified with an RNeasy Protect Mini Kit (QIAGEN) and subjected to qRT–PCR as described for the infected cell samples. .. The HiBiT assay samples from organs were homogenized using a Handy Sonic in lysis buffer (100 mM Tris-HCl, 2 mM EDTA, 0.1% Triton X-100), and the supernatants obtained after centrifugation at 14000 × g for 30 min at 4°C were measured using a BCA protein kit (Nacalai Tesque) to determine the protein concentration, after which 10 μg of protein from the supernatants was used for the HiBiT assay.



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    Composite images of ADGE <t>microarray</t> and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using PCR with the primers having a Taq I site at the end. After cut with Taq I , the same amount of DNA for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
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    Illustration of the <t>microarray</t> experiment. The various steps of the experiment and the corresponding covariates used in the model are listed with their symbols. The model consists of three levels: (i) selection, (ii) scanning and (iii) measurement. In (i), K g 1 and K g 2 mRNA molecules for gene g present in sample 1 and 2 undergo a selection process. Each molecule succeeds or fails in each of the experimental steps: cDNA synthesis, dye labelling, purification, hybridization and washing. Success for each molecule is modelled as a Bernoulli coin toss. The success probability depends on properties of the molecule and of the experiment (covariates). Molecules of the same gene can have different covariates, e.g. if they hybridize on different spots with different probes. If probe is in excess, molecules can be modelled as independent variables and the number of remaining molecules after each step is binomially distributed. The probability of successfully passing through the entire experiment is the product of the probabilities of surviving each individual step. Nested binomial variables are binomial and the final number of molecules ready for being scanned is binomial with two parameters: the unknown original number of transcripts per gene in each sample and the selection probability, modelled as in . Level (ii) describes the translation of the bound molecules remaining after washing ( H s t , a , on array a , spot s , for sample t = 1, 2) into fluorescence intensities, as in . Measurement error (iii) of pixel-wise intensities L j , s t , a (on array a , pixel j on spot s for sample t = 1, 2) is assumed to be normally distributed as in . This model allows to obtain estimates of absolute concentrations K g 1 and K g 2 together with their posterior marginal probability density, as sketched at the bottom.
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    Image Search Results


    Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using PCR with the primers having a Taq I site at the end. After cut with Taq I , the same amount of DNA for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.

    Journal: BMC Genomics

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

    doi: 10.1186/1471-2164-4-28

    Figure Lengend Snippet: Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using PCR with the primers having a Taq I site at the end. After cut with Taq I , the same amount of DNA for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.

    Article Snippet: Regular microarray was carried out following the manufacturer's instructions for the FairPlay™ microarray labeling kit (Stratagene, La Jolla, CA).

    Techniques: Microarray, Clone Assay, Amplification

    Relationship between detected ratios (y) and input ratios (x). The relationship is y = 1.05x 1.55 with R 2 = 0.97 for ADGE microarray while it is y = 0.56x +0.39 with R 2 = 0.96 for regular microarray within the input ratio of 4 (panel A). Within the input ratio range of 1~20, the relationship is y = 15.99ln(x) - 6.14 with R 2 = 0.84 for ADGE-microarray (panel B).

    Journal: BMC Genomics

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

    doi: 10.1186/1471-2164-4-28

    Figure Lengend Snippet: Relationship between detected ratios (y) and input ratios (x). The relationship is y = 1.05x 1.55 with R 2 = 0.97 for ADGE microarray while it is y = 0.56x +0.39 with R 2 = 0.96 for regular microarray within the input ratio of 4 (panel A). Within the input ratio range of 1~20, the relationship is y = 15.99ln(x) - 6.14 with R 2 = 0.84 for ADGE-microarray (panel B).

    Article Snippet: Regular microarray was carried out following the manufacturer's instructions for the FairPlay™ microarray labeling kit (Stratagene, La Jolla, CA).

    Techniques: Microarray

    The MA plots of ADGE microarray and regular microarray. A is the average of log 2 Cy5 and log 2 Cy3, representing intensities of spots. M is the difference of log 2 Cy5 and log 2 Cy3, representing the expression ratios in the power of 2, with positive values for up-regulated genes, negative values for down-regulated genes and 0 for unchanged genes. Panel A: ADGE microarray with HL60 vs HL60/TLK286, average of three replicates. Panel B: regular microarray with HL60 vs HL60/TLK286, average of three replicates. Panel C: ADGE microarray with HL60 vs HL60, average of two replicates. Panel D: number of differential genes selected from all genes on the chip or from genes with > 99% confidence level.

    Journal: BMC Genomics

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

    doi: 10.1186/1471-2164-4-28

    Figure Lengend Snippet: The MA plots of ADGE microarray and regular microarray. A is the average of log 2 Cy5 and log 2 Cy3, representing intensities of spots. M is the difference of log 2 Cy5 and log 2 Cy3, representing the expression ratios in the power of 2, with positive values for up-regulated genes, negative values for down-regulated genes and 0 for unchanged genes. Panel A: ADGE microarray with HL60 vs HL60/TLK286, average of three replicates. Panel B: regular microarray with HL60 vs HL60/TLK286, average of three replicates. Panel C: ADGE microarray with HL60 vs HL60, average of two replicates. Panel D: number of differential genes selected from all genes on the chip or from genes with > 99% confidence level.

    Article Snippet: Regular microarray was carried out following the manufacturer's instructions for the FairPlay™ microarray labeling kit (Stratagene, La Jolla, CA).

    Techniques: Microarray, Expressing

    The variances of Cy3 (HL60) (panel A) and Cy5 (HL60/TLK286) (panel B) in ADGE microarray and regular microarray. The normalized values of Cy3 and Cy5 were used to calculate the variances. Panel C is the number of genes with confidence levels of 90% or greater in ADGE microarray and regular microarray. The confidence level is a result of t-test for each gene.

    Journal: BMC Genomics

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

    doi: 10.1186/1471-2164-4-28

    Figure Lengend Snippet: The variances of Cy3 (HL60) (panel A) and Cy5 (HL60/TLK286) (panel B) in ADGE microarray and regular microarray. The normalized values of Cy3 and Cy5 were used to calculate the variances. Panel C is the number of genes with confidence levels of 90% or greater in ADGE microarray and regular microarray. The confidence level is a result of t-test for each gene.

    Article Snippet: Regular microarray was carried out following the manufacturer's instructions for the FairPlay™ microarray labeling kit (Stratagene, La Jolla, CA).

    Techniques: Microarray

    Comparison of ADGE  microarray  results between two duplicated spots

    Journal: BMC Genomics

    Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

    doi: 10.1186/1471-2164-4-28

    Figure Lengend Snippet: Comparison of ADGE microarray results between two duplicated spots

    Article Snippet: Regular microarray was carried out following the manufacturer's instructions for the FairPlay™ microarray labeling kit (Stratagene, La Jolla, CA).

    Techniques: Microarray

    Illustration of the microarray experiment. The various steps of the experiment and the corresponding covariates used in the model are listed with their symbols. The model consists of three levels: (i) selection, (ii) scanning and (iii) measurement. In (i), K g 1 and K g 2 mRNA molecules for gene g present in sample 1 and 2 undergo a selection process. Each molecule succeeds or fails in each of the experimental steps: cDNA synthesis, dye labelling, purification, hybridization and washing. Success for each molecule is modelled as a Bernoulli coin toss. The success probability depends on properties of the molecule and of the experiment (covariates). Molecules of the same gene can have different covariates, e.g. if they hybridize on different spots with different probes. If probe is in excess, molecules can be modelled as independent variables and the number of remaining molecules after each step is binomially distributed. The probability of successfully passing through the entire experiment is the product of the probabilities of surviving each individual step. Nested binomial variables are binomial and the final number of molecules ready for being scanned is binomial with two parameters: the unknown original number of transcripts per gene in each sample and the selection probability, modelled as in . Level (ii) describes the translation of the bound molecules remaining after washing ( H s t , a , on array a , spot s , for sample t = 1, 2) into fluorescence intensities, as in . Measurement error (iii) of pixel-wise intensities L j , s t , a (on array a , pixel j on spot s for sample t = 1, 2) is assumed to be normally distributed as in . This model allows to obtain estimates of absolute concentrations K g 1 and K g 2 together with their posterior marginal probability density, as sketched at the bottom.

    Journal: Nucleic Acids Research

    Article Title: Genome-wide estimation of transcript concentrations from spotted cDNA microarray data

    doi: 10.1093/nar/gni141

    Figure Lengend Snippet: Illustration of the microarray experiment. The various steps of the experiment and the corresponding covariates used in the model are listed with their symbols. The model consists of three levels: (i) selection, (ii) scanning and (iii) measurement. In (i), K g 1 and K g 2 mRNA molecules for gene g present in sample 1 and 2 undergo a selection process. Each molecule succeeds or fails in each of the experimental steps: cDNA synthesis, dye labelling, purification, hybridization and washing. Success for each molecule is modelled as a Bernoulli coin toss. The success probability depends on properties of the molecule and of the experiment (covariates). Molecules of the same gene can have different covariates, e.g. if they hybridize on different spots with different probes. If probe is in excess, molecules can be modelled as independent variables and the number of remaining molecules after each step is binomially distributed. The probability of successfully passing through the entire experiment is the product of the probabilities of surviving each individual step. Nested binomial variables are binomial and the final number of molecules ready for being scanned is binomial with two parameters: the unknown original number of transcripts per gene in each sample and the selection probability, modelled as in . Level (ii) describes the translation of the bound molecules remaining after washing ( H s t , a , on array a , spot s , for sample t = 1, 2) into fluorescence intensities, as in . Measurement error (iii) of pixel-wise intensities L j , s t , a (on array a , pixel j on spot s for sample t = 1, 2) is assumed to be normally distributed as in . This model allows to obtain estimates of absolute concentrations K g 1 and K g 2 together with their posterior marginal probability density, as sketched at the bottom.

    Article Snippet: Labelled cDNA was synthesized from 20 µg total RNA using Superscript II transcriptase (Life technologies) and Fairplay Microarray Labeling kit (Stratagene) in the presence of either Cy3-dUTP or Cy5-dUTP (Amersham Pharmacia).

    Techniques: Microarray, Selection, Purification, Hybridization, Fluorescence

    Validation of the microarray results . The transcriptomic results obtained by microarray hybridisation were validated by quantitive RT-PCR (qRT-PCR) analysis. The level of differential expression of 12 genes was compared and showed a correlation between microarray (Y-axis) and qRT-PCR analysis (X-axis). The level of differential expression between iron replete and iron limitation is given in Log 2 -values.

    Journal: BMC Genomics

    Article Title: Investigation of the human pathogen Acinetobacter baumannii under iron limiting conditions

    doi: 10.1186/1471-2164-12-126

    Figure Lengend Snippet: Validation of the microarray results . The transcriptomic results obtained by microarray hybridisation were validated by quantitive RT-PCR (qRT-PCR) analysis. The level of differential expression of 12 genes was compared and showed a correlation between microarray (Y-axis) and qRT-PCR analysis (X-axis). The level of differential expression between iron replete and iron limitation is given in Log 2 -values.

    Article Snippet: Total RNA was reverse transcribed and labelled with either Cy3 or Cy5 using the Agilent Fairplay Microarray Labelling kit (Stratagene).

    Techniques: Microarray, Hybridization, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

    Microarray results displayed by COG-function . Depiction of cluster of orthologous groups (COG) and the percentage of up-regulated (green) and down-regulated genes (red) within such group determined by microarray. The total number of genes per COG is shown in parentheses.

    Journal: BMC Genomics

    Article Title: Investigation of the human pathogen Acinetobacter baumannii under iron limiting conditions

    doi: 10.1186/1471-2164-12-126

    Figure Lengend Snippet: Microarray results displayed by COG-function . Depiction of cluster of orthologous groups (COG) and the percentage of up-regulated (green) and down-regulated genes (red) within such group determined by microarray. The total number of genes per COG is shown in parentheses.

    Article Snippet: Total RNA was reverse transcribed and labelled with either Cy3 or Cy5 using the Agilent Fairplay Microarray Labelling kit (Stratagene).

    Techniques: Microarray

    The effect of NaCl on the expression of EF0282, EF1211 and EF2642 as quantified by QPCR (□; Pfaffl method), and by microarray (▪).

    Journal: PLoS ONE

    Article Title: Transcriptomic and Functional Analysis of NaCl-Induced Stress in Enterococcus faecalis

    doi: 10.1371/journal.pone.0094571

    Figure Lengend Snippet: The effect of NaCl on the expression of EF0282, EF1211 and EF2642 as quantified by QPCR (□; Pfaffl method), and by microarray (▪).

    Article Snippet: The concentrations of the RNA samples were measured by using the NanoDrop (NanoDrop Technologies), and the quality was assessed by using the RNA 600 Nano LabChip kit and the Bioanalyzer 2100 (Agilent Technologies). cDNA was synthesized and labeled with the Fairplay II Microarray labeling kit (Stratagene), with modifications as previously described .

    Techniques: Expressing, Microarray