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(A–C) Western blot of transfected-cell lysates expressing the indicated FLAG-tagged PR-mutant constructs using an <t>anti-FLAG</t> antibody. β-Actin served as a loading control. (D–F) Western blot analyses of virions pelleted from equal volumes of the corresponding culture supernatants using an anti-FLAG antibody. Molecular masses are indicated in kDa.
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Mimotopes epitope p4448
(A–C) Western blot of transfected-cell lysates expressing the indicated FLAG-tagged PR-mutant constructs using an <t>anti-FLAG</t> antibody. β-Actin served as a loading control. (D–F) Western blot analyses of virions pelleted from equal volumes of the corresponding culture supernatants using an anti-FLAG antibody. Molecular masses are indicated in kDa.
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Image Search Results


(A–C) Western blot of transfected-cell lysates expressing the indicated FLAG-tagged PR-mutant constructs using an anti-FLAG antibody. β-Actin served as a loading control. (D–F) Western blot analyses of virions pelleted from equal volumes of the corresponding culture supernatants using an anti-FLAG antibody. Molecular masses are indicated in kDa.

Journal: bioRxiv

Article Title: Multiple Pol determinants constrain koala retrovirus A replication

doi: 10.64898/2026.10.13.759007

Figure Lengend Snippet: (A–C) Western blot of transfected-cell lysates expressing the indicated FLAG-tagged PR-mutant constructs using an anti-FLAG antibody. β-Actin served as a loading control. (D–F) Western blot analyses of virions pelleted from equal volumes of the corresponding culture supernatants using an anti-FLAG antibody. Molecular masses are indicated in kDa.

Article Snippet: Primary antibodies included anti-FLAG epitope (1:1,000; Novus Biologicals; cat. no. NB600-345), anti-GFP (1:1,000; Takara Bio; cat. no. 632381), anti-β-actin (1:1,000; Abcam; cat. no. ab8226), anti-KoRV-A CA, anti-KoRV-A MA, anti-KoRV-A RT, anti-KoRV-A IN, and anti-MLV CA (clone R187).

Techniques: Western Blot, Transfection, Expressing, Mutagenesis, Construct, Control

(A) Schematic representation of the proviral constructs containing a FLAG epitope within p12 or RT in the KoRV-A and MLV backgrounds. (B) Exogenous RT activity in culture supernatants from HEK293T cells transfected with the corresponding untagged, p12-FLAG, or RT-FLAG proviral constructs. Data are presented as geometric means ± geometric standard deviations, with individual biological replicates shown as points. Statistical significance was evaluated by ordinary two-way ANOVA using log10-transformed values followed by Šídák’s multiple-comparisons test. Exact adjusted p values are shown. (C–E) Western blot analyses of transfected-cell lysates using an anti-FLAG antibody. β-Actin served as a loading control. (F–H) Western blot analyses of virions pelleted from equal volumes of the corresponding culture supernatants using an anti-FLAG antibody. Molecular masses are indicated in kDa.

Journal: bioRxiv

Article Title: Multiple Pol determinants constrain koala retrovirus A replication

doi: 10.64898/2026.10.13.759007

Figure Lengend Snippet: (A) Schematic representation of the proviral constructs containing a FLAG epitope within p12 or RT in the KoRV-A and MLV backgrounds. (B) Exogenous RT activity in culture supernatants from HEK293T cells transfected with the corresponding untagged, p12-FLAG, or RT-FLAG proviral constructs. Data are presented as geometric means ± geometric standard deviations, with individual biological replicates shown as points. Statistical significance was evaluated by ordinary two-way ANOVA using log10-transformed values followed by Šídák’s multiple-comparisons test. Exact adjusted p values are shown. (C–E) Western blot analyses of transfected-cell lysates using an anti-FLAG antibody. β-Actin served as a loading control. (F–H) Western blot analyses of virions pelleted from equal volumes of the corresponding culture supernatants using an anti-FLAG antibody. Molecular masses are indicated in kDa.

Article Snippet: Primary antibodies included anti-FLAG epitope (1:1,000; Novus Biologicals; cat. no. NB600-345), anti-GFP (1:1,000; Takara Bio; cat. no. 632381), anti-β-actin (1:1,000; Abcam; cat. no. ab8226), anti-KoRV-A CA, anti-KoRV-A MA, anti-KoRV-A RT, anti-KoRV-A IN, and anti-MLV CA (clone R187).

Techniques: Construct, FLAG-tag, Activity Assay, Transfection, Transformation Assay, Western Blot, Control