Journal: iScience

Article Title: tANCHOR-cell-based assay for monitoring of SARS-CoV-2 neutralizing antibodies rapidly adaptive to various receptor-binding domains

doi: 10.1016/j.isci.2024.109123

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Article Snippet: Rabbit anti-V5 , Novus Biologicals , Cat# NB600-381.

Techniques: Virus, Recombinant, Saline, Staining, Isolation, Protease Inhibitor, Software, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5

doi: 10.1016/j.celrep.2023.113145

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Article Snippet: MYC Epitope Tag Antibody Rabbit Polyclonal , Rockland , Cat#600-401-381; RRID:AB_217927.

Techniques: Control, Recombinant, Affinity Purification, Virus, Plasmid Preparation, Quantitative Proteomics, Over Expression, Knock-Out, Software

Journal: PLoS Pathogens

Article Title: The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid

doi: 10.1371/journal.ppat.1005833

Figure Lengend Snippet: (A) A3-RNA cross-linked complexes were immunoprecipitated from mock infected or HIV-1 NL4-3 ΔVif infected HEK 293T cells stably expressing 3×HA-tagged A3F, A3G or A3H proteins that had been fed with 4SU and UV-irradiated. A3-RNA cross-linked complexes were also immunoprecipitated from purified HIV-1 NL4-3 ΔVif virions that were derived from the 4SU-fed infected cells. Complexes were visualized by autoradiography (top) and Western blot analysis using a polyclonal anti-HA (bottom). (B) Frequency distribution of nucleotide occurrence (read density) in reads that were mapped to the HIV-1 NL4-3 genome (left). A colinear schematic diagram of the HIV-1 genome is presented above. Correlation analyses (see ) of A3 binding to viral RNA in cells from two independent CLIP experiments are shown (right).

Article Snippet: The rabbit polyclonal anti-HA (600-401-384 Rockland) and the mouse monoclonal anti-HIV-1 p24CA (183-H12-5C, NIH AIDS Reagent Program) were used in Western blot analyses.

Techniques: Immunoprecipitation, Infection, Stable Transfection, Expressing, Irradiation, Purification, Derivative Assay, Autoradiography, Western Blot, Binding Assay