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eclipse te2000 5 microscope  (Nikon)


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    Nikon eclipse te2000 5 microscope
    Eclipse Te2000 5 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 60152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te2000+5+microscope/Objectives/pm37731092-131-16-15
    Average 99 stars, based on 60152 article reviews
    eclipse te2000 5 microscope - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Live Cell Imaging:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Microscopy:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Fluorescence:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Electron Microscopy:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Immunohistochemical staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Confocal:

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Single Cell:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Clone Assay:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Process/Product Development:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..



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    Image Search Results


    (A) Representative epifluorescence images (20×) of L4 animals expressing Pins-3::GFP under basal conditions (20 °C on Nematode Growth Media plates seeded with OP50), under oxidative stress (1 mM FeSO 4, 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous tyramine (15 mM). Scale bar 50 µm. (B) Corresponding quantification of fluorescence levels per worm. Scatter dot plot with relative expression of Pins-3::GFP normalized to basal conditions of each independent experiment. Line at the median. n = 50−200 animals per condition (distributed across 3–4 independent experiments). One-way ANOVA and Dunn’s post hoc test vs. basal were used. * p < 0.05, **** p < 0.0001. (C) Log 2 fold-changes in ins-3 transcript levels in animals exposed to oxidative stress (1 mM FeSO 4 , 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous Tyramine (15 mM). Negative and positive values indicate down- and up-regulation of ins-3 , respectively. Fold change was calculated as ΔCt basal conditions/ΔCt test conditions. Results are shown as mean ± s.e.m. n = 4 independent experiments. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Journal: PLOS Biology

    Article Title: The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response

    doi: 10.1371/journal.pbio.3002997

    Figure Lengend Snippet: (A) Representative epifluorescence images (20×) of L4 animals expressing Pins-3::GFP under basal conditions (20 °C on Nematode Growth Media plates seeded with OP50), under oxidative stress (1 mM FeSO 4, 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous tyramine (15 mM). Scale bar 50 µm. (B) Corresponding quantification of fluorescence levels per worm. Scatter dot plot with relative expression of Pins-3::GFP normalized to basal conditions of each independent experiment. Line at the median. n = 50−200 animals per condition (distributed across 3–4 independent experiments). One-way ANOVA and Dunn’s post hoc test vs. basal were used. * p < 0.05, **** p < 0.0001. (C) Log 2 fold-changes in ins-3 transcript levels in animals exposed to oxidative stress (1 mM FeSO 4 , 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous Tyramine (15 mM). Negative and positive values indicate down- and up-regulation of ins-3 , respectively. Fold change was calculated as ΔCt basal conditions/ΔCt test conditions. Results are shown as mean ± s.e.m. n = 4 independent experiments. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Article Snippet: For ins-3 and ins-4 expression levels analysis, animals containing the corresponding transcriptional GFP reporter ( Pins-3::GFP and Pins-4:GFP , respectively) were imaged using an epifluorescence microscope (Nikon Eclipse TE2000-5) coupled to a CCD camera (Nikon DS-Qi2) with 20× objective.

    Techniques: Expressing, Fluorescence

    Representative epifluorescence images (40×) of young not gravid adults expressing Pges-1::INS-3::VENUS in wild-type and tyra-3 null mutant background, in the absence or presence of exogenous tyramine (15 mM). Scale bar 25 µm. Fluorescence is observed in the two posterior coelomocytes of worms (inset). Right. Corresponding quantification of fluorescence intensity on the coelomocytes (normalized to wild-type animals without tyramine exposure). Line at the median. n = 35–40 animals per condition distributed across three independent experiments. For conditions with tyramine, a two-tailed Student’s t test vs. the same strain without tyramine was used. * p < 0.05. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Journal: PLOS Biology

    Article Title: The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response

    doi: 10.1371/journal.pbio.3002997

    Figure Lengend Snippet: Representative epifluorescence images (40×) of young not gravid adults expressing Pges-1::INS-3::VENUS in wild-type and tyra-3 null mutant background, in the absence or presence of exogenous tyramine (15 mM). Scale bar 25 µm. Fluorescence is observed in the two posterior coelomocytes of worms (inset). Right. Corresponding quantification of fluorescence intensity on the coelomocytes (normalized to wild-type animals without tyramine exposure). Line at the median. n = 35–40 animals per condition distributed across three independent experiments. For conditions with tyramine, a two-tailed Student’s t test vs. the same strain without tyramine was used. * p < 0.05. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Article Snippet: For ins-3 and ins-4 expression levels analysis, animals containing the corresponding transcriptional GFP reporter ( Pins-3::GFP and Pins-4:GFP , respectively) were imaged using an epifluorescence microscope (Nikon Eclipse TE2000-5) coupled to a CCD camera (Nikon DS-Qi2) with 20× objective.

    Techniques: Expressing, Mutagenesis, Fluorescence, Two Tailed Test