fluorescence inverted microscope (Nikon)
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Nikon
fluorescence inverted microscope
Fluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ni-e+fluorescent+microscope/Inverted+Microscopes/pm40077620-55-35-38
Average 99 stars, based on 14758 article reviews
Fluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ni-e+fluorescent+microscope/Inverted+Microscopes/pm40077620-55-35-38
Average 99 stars, based on 14758 article reviews
fluorescence inverted microscope - by Bioz Stars,
2026-10
99/100 stars
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To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration. 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![a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1497/pmc12271497/pmc12271497__41467_2025_61442_Fig2_HTML.jpg)
