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bioMerieux gmbh gradient diffusion method etest
Gradient Diffusion Method Etest, supplied by bioMerieux gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e-test+diffusion/etest+gradient+diffusion/pmc04331070-85-17-21
Average 90 stars, based on 1 article reviews
gradient diffusion method etest - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Streptococcus pneumoniae serotypes with meningeal tropism in cases of invasive pneumococcal disease. Community of Madrid, 2007–2018
Article Snippet: Introduction: To describe the distribution of Streptococcus pneumoniae serotypes isolated in cerebrospinal fluid (CSF) between 2007–2018 in the Community of Madrid (CM) and to identify those with higher meningeal tropism.. Methods: Strains isolated from invasive pneumococcal disease were sent to the Regional Laboratory of Public Health by Microbiology laboratories of public and private hospitals of the CM.. The frequency of serotypes from CSF was compared with that observed in other samples.

Article Title: Elevated Staphylococcus Ceftriaxone MICs are an Etest Artifact
Article Snippet: The recent publication by Pickering et al [ 1 ] described a collection of methicillin-susceptible Staphylococcus aureus (MSSA) that displayed elevated ceftriaxone minimum inhibitory concentrations (MICs) when tested by Etest (bioMerieux, Durham, North Carolina) gradient diffusion and would have been called “Resistant” to ceftriaxone based on previous Clinical and Laboratory Standards Institute (CLSI) interpretive guidance.

Article Title: Echinocandin Resistance in Candida Species: a Review of Recent Developments.
Article Snippet: The echinocandins are important agents for the treatment of invasive fungal infections, especially those caused by Candida species.. However, as with other antimicrobial agents, microbiologic resistance to this class of antifungal agents has emerged and can result in clinical failure.. Several studies have recently reported an increase in echinocandin resistance in Candida glabrata isolates at various medical centers in different geographic regions of the USA.

Diffusion-based Assay:

Article Title: Carbapenem-resistant Klebsiella pneumoniae outbreak with monoclonal spread: Evaluation of resistance genes and ceftazidime-avibactam susceptibility.
Article Snippet: The VITEK-2 (BioMerieux) automated system was used to determine antibiotic susceptibility. .. Isolates found resistant to all ertapenem, imipenem, and meropenem antibiotics were also confirmed by gradient diffusion test (Etest, BioMerieux, France). ..

Article Title: Rapid Emergence of Daptomycin Resistance in Clinical Isolates of Corynebacterium striatum … A Cautionary Tale
Article Snippet: Antibiotic susceptibility profiles for the case patient's three C. striatum isolates ( ) were determined using the gradient diffusion method Etest (bioMerieux, Durham, NC) on standard Mueller-Hinton agar containing 5% sheep blood (Remel) incubated in ambient air for 24 to 48 h. Quality control of reagents and medium and interpretation of MIC values were performed according to Clinical and Laboratory Standards Institute (CLSI) standards. .. Antibiotic susceptibility profiles for the case patient's three C. striatum isolates ( ) were determined using the gradient diffusion method Etest (bioMerieux, Durham, NC) on standard Mueller-Hinton agar containing 5% sheep blood (Remel) incubated in ambient air for 24 to 48 h. Quality control of reagents and medium and interpretation of MIC values were performed according to Clinical and Laboratory Standards Institute (CLSI) standards. ..

Article Title: Antibiotic resistance genotype, phenotype, and clinical outcomes in patients with Gram-negative infections at Rabin Medical Center in Israel.
Article Snippet: Antibiotic resistance is a major cause of morbidity and mortality.. However, a better understanding of the relationship between bacterial genetic markers, phenotypic resistance, and clinical outcomes is needed.. We performed whole-genome sequencing on five medically important pathogens (Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa) to investigate how resistance genes impact patient outcomes.

Article Title: Antibiotic resistance genotype, phenotype, and clinical outcomes in patients with Gram-negative infections at Rabin Medical Center in Israel
Article Snippet: The MIC plates were read at OD 600nm (Cytation5, Biotek, Winooski, VT). .. For sulbactam–durlobactam, Etest (bioMerieux, Marcy-l’Etoile, France) gradient diffusion was used, with MICs identified from the zone of inhibition. ..

Article Title: When Good Bugs Go Bad: Epidemiology and Antimicrobial Resistance Profiles of Corynebacterium striatum, an Emerging Multidrug-Resistant, Opportunistic Pathogen
Article Snippet: .. 293 294 Antimicrobial Susceptibility Testing: 295 For the all isolates included in the study, susceptibility testing was initially 296 performed when deemed clinically appropriate as a part of routine patient care using 297 gradient diffusion Etest (bioMerieux, Durham, NC). ..

Incubation:

Article Title: Rapid Emergence of Daptomycin Resistance in Clinical Isolates of Corynebacterium striatum … A Cautionary Tale
Article Snippet: Antibiotic susceptibility profiles for the case patient's three C. striatum isolates ( ) were determined using the gradient diffusion method Etest (bioMerieux, Durham, NC) on standard Mueller-Hinton agar containing 5% sheep blood (Remel) incubated in ambient air for 24 to 48 h. Quality control of reagents and medium and interpretation of MIC values were performed according to Clinical and Laboratory Standards Institute (CLSI) standards. .. Antibiotic susceptibility profiles for the case patient's three C. striatum isolates ( ) were determined using the gradient diffusion method Etest (bioMerieux, Durham, NC) on standard Mueller-Hinton agar containing 5% sheep blood (Remel) incubated in ambient air for 24 to 48 h. Quality control of reagents and medium and interpretation of MIC values were performed according to Clinical and Laboratory Standards Institute (CLSI) standards. ..

Control:

Article Title: Rapid Emergence of Daptomycin Resistance in Clinical Isolates of Corynebacterium striatum … A Cautionary Tale
Article Snippet: Antibiotic susceptibility profiles for the case patient's three C. striatum isolates ( ) were determined using the gradient diffusion method Etest (bioMerieux, Durham, NC) on standard Mueller-Hinton agar containing 5% sheep blood (Remel) incubated in ambient air for 24 to 48 h. Quality control of reagents and medium and interpretation of MIC values were performed according to Clinical and Laboratory Standards Institute (CLSI) standards. .. Antibiotic susceptibility profiles for the case patient's three C. striatum isolates ( ) were determined using the gradient diffusion method Etest (bioMerieux, Durham, NC) on standard Mueller-Hinton agar containing 5% sheep blood (Remel) incubated in ambient air for 24 to 48 h. Quality control of reagents and medium and interpretation of MIC values were performed according to Clinical and Laboratory Standards Institute (CLSI) standards. ..

Inhibition:

Article Title: Antibiotic resistance genotype, phenotype, and clinical outcomes in patients with Gram-negative infections at Rabin Medical Center in Israel.
Article Snippet: Antibiotic resistance is a major cause of morbidity and mortality.. However, a better understanding of the relationship between bacterial genetic markers, phenotypic resistance, and clinical outcomes is needed.. We performed whole-genome sequencing on five medically important pathogens (Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa) to investigate how resistance genes impact patient outcomes.

Article Title: Antibiotic resistance genotype, phenotype, and clinical outcomes in patients with Gram-negative infections at Rabin Medical Center in Israel
Article Snippet: The MIC plates were read at OD 600nm (Cytation5, Biotek, Winooski, VT). .. For sulbactam–durlobactam, Etest (bioMerieux, Marcy-l’Etoile, France) gradient diffusion was used, with MICs identified from the zone of inhibition. ..



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In vitro activity of  cefiderocol  in relation to the activity of the other drugs studied. S = susceptible; R = resistant.

Journal: Antibiotics

Article Title: Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant Pseudomonas aeruginosa Strains: Influence of Bacterial Inoculum

doi: 10.3390/antibiotics13070663

Figure Lengend Snippet: In vitro activity of cefiderocol in relation to the activity of the other drugs studied. S = susceptible; R = resistant.

Article Snippet: Diffusion gradient strips E-test Liofilchem ® of cefiderocol: This technique was carried out according to the manufacturer’s instructions using three different bacterial inoculums.

Techniques: In Vitro, Activity Assay

Results of MIC50 (mg/L) and MIC90 (mg/L) values for  cefiderocol  compared to other drugs.

Journal: Antibiotics

Article Title: Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant Pseudomonas aeruginosa Strains: Influence of Bacterial Inoculum

doi: 10.3390/antibiotics13070663

Figure Lengend Snippet: Results of MIC50 (mg/L) and MIC90 (mg/L) values for cefiderocol compared to other drugs.

Article Snippet: Diffusion gradient strips E-test Liofilchem ® of cefiderocol: This technique was carried out according to the manufacturer’s instructions using three different bacterial inoculums.

Techniques:

Comparison of microdilution in a medium with iron depletion: UMIC ® Bruker with Thermo Scientific™ Oxoid™ cefiderocol antimicrobial susceptibility discs. (ATU: Area of technical uncertainty).

Journal: Antibiotics

Article Title: Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant Pseudomonas aeruginosa Strains: Influence of Bacterial Inoculum

doi: 10.3390/antibiotics13070663

Figure Lengend Snippet: Comparison of microdilution in a medium with iron depletion: UMIC ® Bruker with Thermo Scientific™ Oxoid™ cefiderocol antimicrobial susceptibility discs. (ATU: Area of technical uncertainty).

Article Snippet: Diffusion gradient strips E-test Liofilchem ® of cefiderocol: This technique was carried out according to the manufacturer’s instructions using three different bacterial inoculums.

Techniques: Comparison