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Biokine Therapeutics dynafit software
Dynafit Software, supplied by Biokine Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dynafit+software+program/dynafit+software/pmc05314180-790-7-12
Average 90 stars, based on 1 article reviews

dynafit software - by Bioz Stars, 2026-10

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Variant Assay:

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software (47) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described in the Results section. ..

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. With 18 μ m R6-PBP2b, nitrocefin was varied alone from 20 to 100 μ m , whereas it was kept at 10 μ m while amoxicillin ranged from 0 to 25 μ m , penicillin G from 0 to 800 μ m , piperacillin from 0 to 80 μ m , and cefotaxime from 0 to 5 m m . With 20 μ m 5204-PBP2b, nitrocefin was varied alone from 100 to 500 μ m , whereas it was kept at 150 μ m while amoxicillin and penicillin G ranged from 0 to 2.5 m m , and piperacillin from 0 to 800 μ m . With 30 μ m Hybrid-PBP2b, nitrocefin was varied alone from 200 to 800 μ m , whereas it was kept at 70 μ m while amoxicillin ranged from 0 to 1.2 m m , penicillin G from 0 to 2 m m and piperacillin from 0 to 150 μ m . With 20 μ m T446A-PBP2b, nitrocefin was varied alone from 70 to 200 μ m , whereas it was kept at 35 μ m while amoxicillin ranged from 0 to 625 μ m , penicillin G from 0 to 2.4 m m , piperacillin from 0 to 300 μ m , and cefotaxime from 0 to 4.5 m m . For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software ( 47 ) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described under “Results.” Thermofluor Assay Soluble PBP2b variants at a final concentration of 0.5 mg/ml (∼6 μ m ) were incubated with or without 1 m m of amoxicillin, penicillin G, piperacillin, or cefotaxime in 50 m m HEPES, pH 7.5, 150 m m NaCl, and 10 m m MgCl 2 for 5 min at room temperature prior to the addition of SYPRO® Orange (12× final concentration) on ice. .. Fluorescence melting curves were recorded in the SYBR channel in a CFX Connect instrument (Bio-Rad) with a 20–100 °C temperature ramp (0.5 °C steps of 7.2 s) and analyzed with the CFX Manager software.

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. With 18 μ m R6-PBP2b, nitrocefin was varied alone from 20 to 100 μ m , whereas it was kept at 10 μ m while amoxicillin ranged from 0 to 25 μ m , penicillin G from 0 to 800 μ m , piperacillin from 0 to 80 μ m , and cefotaxime from 0 to 5 m m . With 20 μ m 5204-PBP2b, nitrocefin was varied alone from 100 to 500 μ m , whereas it was kept at 150 μ m while amoxicillin and penicillin G ranged from 0 to 2.5 m m , and piperacillin from 0 to 800 μ m . With 30 μ m Hybrid-PBP2b, nitrocefin was varied alone from 200 to 800 μ m , whereas it was kept at 70 μ m while amoxicillin ranged from 0 to 1.2 m m , penicillin G from 0 to 2 m m and piperacillin from 0 to 150 μ m . With 20 μ m T446A-PBP2b, nitrocefin was varied alone from 70 to 200 μ m , whereas it was kept at 35 μ m while amoxicillin ranged from 0 to 625 μ m , penicillin G from 0 to 2.4 m m , piperacillin from 0 to 300 μ m , and cefotaxime from 0 to 4.5 m m . For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software ( 47 ) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described under “Results.” ..

Software:

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software (47) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described in the Results section. ..

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: Imaging and quantification were performed using a GelDoc ChemiDoc MP imager and the Image Lab software (Bio-Rad). .. The data were fitted globally with the DynaFit software ( 47 ) (Biokine) using a second order reaction model. ..

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. With 18 μ m R6-PBP2b, nitrocefin was varied alone from 20 to 100 μ m , whereas it was kept at 10 μ m while amoxicillin ranged from 0 to 25 μ m , penicillin G from 0 to 800 μ m , piperacillin from 0 to 80 μ m , and cefotaxime from 0 to 5 m m . With 20 μ m 5204-PBP2b, nitrocefin was varied alone from 100 to 500 μ m , whereas it was kept at 150 μ m while amoxicillin and penicillin G ranged from 0 to 2.5 m m , and piperacillin from 0 to 800 μ m . With 30 μ m Hybrid-PBP2b, nitrocefin was varied alone from 200 to 800 μ m , whereas it was kept at 70 μ m while amoxicillin ranged from 0 to 1.2 m m , penicillin G from 0 to 2 m m and piperacillin from 0 to 150 μ m . With 20 μ m T446A-PBP2b, nitrocefin was varied alone from 70 to 200 μ m , whereas it was kept at 35 μ m while amoxicillin ranged from 0 to 625 μ m , penicillin G from 0 to 2.4 m m , piperacillin from 0 to 300 μ m , and cefotaxime from 0 to 4.5 m m . For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software ( 47 ) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described under “Results.” Thermofluor Assay Soluble PBP2b variants at a final concentration of 0.5 mg/ml (∼6 μ m ) were incubated with or without 1 m m of amoxicillin, penicillin G, piperacillin, or cefotaxime in 50 m m HEPES, pH 7.5, 150 m m NaCl, and 10 m m MgCl 2 for 5 min at room temperature prior to the addition of SYPRO® Orange (12× final concentration) on ice. .. Fluorescence melting curves were recorded in the SYBR channel in a CFX Connect instrument (Bio-Rad) with a 20–100 °C temperature ramp (0.5 °C steps of 7.2 s) and analyzed with the CFX Manager software.

Article Title: Ordered Cleavage of Myeloperoxidase Ester Bonds Releases Active site Heme Leading to Inactivation of Myeloperoxidase by Benzoic Acid Hydrazide Analogs
Article Snippet: .. For the inhibitory effect of ABAH on MPO activity, a set of the time-dependent fluorescence progress curves was fit simultaneously by a slow-tight binding model using DynaFit 3 software ([26]; Biokine, Ltd. Watertown, MA, USA). ..

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: Imaging and quantification were performed using a GelDoc ChemiDoc MP imager and the Image Lab software (BioRad). .. Data were fitted globally with the DynaFit software (47) (Biokine) using a second order reaction model. by guest on January 8, 2017 http://w w w .jbc.org/ D ow nloaded from Transpeptidase activity and β-lactam reactivity of pneumococcal PBP2b 11 Amoxicillin deacylation kinetics—Full- length R6- or 5204-PBP2b were incubated at 1 μM for 15 min at 20°C with 100 μM amoxicillin in 50 mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, and 0.02% (w/v) Triton X-100, prior to addition of 1 mM Bocillin FL at time zero of the deacylation time course. ..

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: Imaging and quantification were performed using a GelDoc ChemiDoc MP imager and the Image Lab software (Bio-Rad). .. The data were fitted globally with the DynaFit software ( 47 ) (Biokine) using a second order reaction model. Amoxicillin Deacylation Kinetics Full-length R6- or 5204-PBP2b were incubated at 1 μ m for 15 min at 20 °C with 100 μ m amoxicillin in 50 m m HEPES, pH 7.5, 150 m m NaCl, 10 m m MgCl 2 , and 0.02% (w/v) Triton X-100, prior to addition of 1 m m Bocillin FL at time 0 of the deacylation time course. ..

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. With 18 μ m R6-PBP2b, nitrocefin was varied alone from 20 to 100 μ m , whereas it was kept at 10 μ m while amoxicillin ranged from 0 to 25 μ m , penicillin G from 0 to 800 μ m , piperacillin from 0 to 80 μ m , and cefotaxime from 0 to 5 m m . With 20 μ m 5204-PBP2b, nitrocefin was varied alone from 100 to 500 μ m , whereas it was kept at 150 μ m while amoxicillin and penicillin G ranged from 0 to 2.5 m m , and piperacillin from 0 to 800 μ m . With 30 μ m Hybrid-PBP2b, nitrocefin was varied alone from 200 to 800 μ m , whereas it was kept at 70 μ m while amoxicillin ranged from 0 to 1.2 m m , penicillin G from 0 to 2 m m and piperacillin from 0 to 150 μ m . With 20 μ m T446A-PBP2b, nitrocefin was varied alone from 70 to 200 μ m , whereas it was kept at 35 μ m while amoxicillin ranged from 0 to 625 μ m , penicillin G from 0 to 2.4 m m , piperacillin from 0 to 300 μ m , and cefotaxime from 0 to 4.5 m m . For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software ( 47 ) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described under “Results.” ..

Concentration Assay:

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. With 18 μ m R6-PBP2b, nitrocefin was varied alone from 20 to 100 μ m , whereas it was kept at 10 μ m while amoxicillin ranged from 0 to 25 μ m , penicillin G from 0 to 800 μ m , piperacillin from 0 to 80 μ m , and cefotaxime from 0 to 5 m m . With 20 μ m 5204-PBP2b, nitrocefin was varied alone from 100 to 500 μ m , whereas it was kept at 150 μ m while amoxicillin and penicillin G ranged from 0 to 2.5 m m , and piperacillin from 0 to 800 μ m . With 30 μ m Hybrid-PBP2b, nitrocefin was varied alone from 200 to 800 μ m , whereas it was kept at 70 μ m while amoxicillin ranged from 0 to 1.2 m m , penicillin G from 0 to 2 m m and piperacillin from 0 to 150 μ m . With 20 μ m T446A-PBP2b, nitrocefin was varied alone from 70 to 200 μ m , whereas it was kept at 35 μ m while amoxicillin ranged from 0 to 625 μ m , penicillin G from 0 to 2.4 m m , piperacillin from 0 to 300 μ m , and cefotaxime from 0 to 4.5 m m . For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software ( 47 ) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described under “Results.” Thermofluor Assay Soluble PBP2b variants at a final concentration of 0.5 mg/ml (∼6 μ m ) were incubated with or without 1 m m of amoxicillin, penicillin G, piperacillin, or cefotaxime in 50 m m HEPES, pH 7.5, 150 m m NaCl, and 10 m m MgCl 2 for 5 min at room temperature prior to the addition of SYPRO® Orange (12× final concentration) on ice. .. Fluorescence melting curves were recorded in the SYBR channel in a CFX Connect instrument (Bio-Rad) with a 20–100 °C temperature ramp (0.5 °C steps of 7.2 s) and analyzed with the CFX Manager software.

Incubation:

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: .. With 18 μ m R6-PBP2b, nitrocefin was varied alone from 20 to 100 μ m , whereas it was kept at 10 μ m while amoxicillin ranged from 0 to 25 μ m , penicillin G from 0 to 800 μ m , piperacillin from 0 to 80 μ m , and cefotaxime from 0 to 5 m m . With 20 μ m 5204-PBP2b, nitrocefin was varied alone from 100 to 500 μ m , whereas it was kept at 150 μ m while amoxicillin and penicillin G ranged from 0 to 2.5 m m , and piperacillin from 0 to 800 μ m . With 30 μ m Hybrid-PBP2b, nitrocefin was varied alone from 200 to 800 μ m , whereas it was kept at 70 μ m while amoxicillin ranged from 0 to 1.2 m m , penicillin G from 0 to 2 m m and piperacillin from 0 to 150 μ m . With 20 μ m T446A-PBP2b, nitrocefin was varied alone from 70 to 200 μ m , whereas it was kept at 35 μ m while amoxicillin ranged from 0 to 625 μ m , penicillin G from 0 to 2.4 m m , piperacillin from 0 to 300 μ m , and cefotaxime from 0 to 4.5 m m . For each PBP2b variant, the data for a range of β-lactam concentrations were fitted globally with the DynaFit software ( 47 ) (Biokine) using second order reaction models for the acylations and first order model for the deacylation, as described under “Results.” Thermofluor Assay Soluble PBP2b variants at a final concentration of 0.5 mg/ml (∼6 μ m ) were incubated with or without 1 m m of amoxicillin, penicillin G, piperacillin, or cefotaxime in 50 m m HEPES, pH 7.5, 150 m m NaCl, and 10 m m MgCl 2 for 5 min at room temperature prior to the addition of SYPRO® Orange (12× final concentration) on ice. .. Fluorescence melting curves were recorded in the SYBR channel in a CFX Connect instrument (Bio-Rad) with a 20–100 °C temperature ramp (0.5 °C steps of 7.2 s) and analyzed with the CFX Manager software.

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: Imaging and quantification were performed using a GelDoc ChemiDoc MP imager and the Image Lab software (BioRad). .. Data were fitted globally with the DynaFit software (47) (Biokine) using a second order reaction model. by guest on January 8, 2017 http://w w w .jbc.org/ D ow nloaded from Transpeptidase activity and β-lactam reactivity of pneumococcal PBP2b 11 Amoxicillin deacylation kinetics—Full- length R6- or 5204-PBP2b were incubated at 1 μM for 15 min at 20°C with 100 μM amoxicillin in 50 mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, and 0.02% (w/v) Triton X-100, prior to addition of 1 mM Bocillin FL at time zero of the deacylation time course. ..

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: Imaging and quantification were performed using a GelDoc ChemiDoc MP imager and the Image Lab software (Bio-Rad). .. The data were fitted globally with the DynaFit software ( 47 ) (Biokine) using a second order reaction model. Amoxicillin Deacylation Kinetics Full-length R6- or 5204-PBP2b were incubated at 1 μ m for 15 min at 20 °C with 100 μ m amoxicillin in 50 m m HEPES, pH 7.5, 150 m m NaCl, 10 m m MgCl 2 , and 0.02% (w/v) Triton X-100, prior to addition of 1 m m Bocillin FL at time 0 of the deacylation time course. ..

Activity Assay:

Article Title: Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity
Article Snippet: Imaging and quantification were performed using a GelDoc ChemiDoc MP imager and the Image Lab software (BioRad). .. Data were fitted globally with the DynaFit software (47) (Biokine) using a second order reaction model. by guest on January 8, 2017 http://w w w .jbc.org/ D ow nloaded from Transpeptidase activity and β-lactam reactivity of pneumococcal PBP2b 11 Amoxicillin deacylation kinetics—Full- length R6- or 5204-PBP2b were incubated at 1 μM for 15 min at 20°C with 100 μM amoxicillin in 50 mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, and 0.02% (w/v) Triton X-100, prior to addition of 1 mM Bocillin FL at time zero of the deacylation time course. ..

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