Review



genopal®-micm mirna arrays  (Mitsubishi Rayon CO)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Mitsubishi Rayon CO genopal®-micm mirna arrays
    The expression levels of miRNAs during male germ cell development. (A) Expression profiling of 12 PGC-enriched miRNAs identified by <t>miRNA</t> microarray in developing male germ cells. For each miRNA, the mean-centered log 2 -expression profile (mean = 0) is shown. A hierarchical clustering was done to visualize the similarity in expression patterns of the miRNAs. The colors represent increased (red) and decreased (green) expression relative to the mean expression level. (B) PGC-enriched expression of miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301 (red) among 12 miRNAs identified from a miRNA microarray analysis were confirmed by miRNA qPCR. (C) Northern blot analysis demonstrates that miR-18a, -93, -106b, and -181a were expressed in PGCs during male germ cell development. In the bottom, Ethidium bromide staining of 28S, 18S, and 5S rRNAs is shown as a loading control. Northern blot assay was performed as described in Materials and Methods. (D) miRNA in situ hybridization assay demonstrated the PGC-enriched expression patterns of miRNAs in PGCs at 12.5 dpc. Arrow, a seminiferous cord; black arrowhead, primordial germ cells; white arrowhead, Sertoli cells.
    Genopal® Micm Mirna Arrays, supplied by Mitsubishi Rayon CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+genopal+mouse+custom+chip/genopal+microarray+system/pmc04625071-39-7-10
    Average 90 stars, based on 1 article reviews
    genopal®-micm mirna arrays - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "PGC-Enriched miRNAs Control Germ Cell Development"

    Article Title: PGC-Enriched miRNAs Control Germ Cell Development

    Journal: Molecules and Cells

    doi: 10.14348/molcells.2015.0146

    The expression levels of miRNAs during male germ cell development. (A) Expression profiling of 12 PGC-enriched miRNAs identified by miRNA microarray in developing male germ cells. For each miRNA, the mean-centered log 2 -expression profile (mean = 0) is shown. A hierarchical clustering was done to visualize the similarity in expression patterns of the miRNAs. The colors represent increased (red) and decreased (green) expression relative to the mean expression level. (B) PGC-enriched expression of miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301 (red) among 12 miRNAs identified from a miRNA microarray analysis were confirmed by miRNA qPCR. (C) Northern blot analysis demonstrates that miR-18a, -93, -106b, and -181a were expressed in PGCs during male germ cell development. In the bottom, Ethidium bromide staining of 28S, 18S, and 5S rRNAs is shown as a loading control. Northern blot assay was performed as described in Materials and Methods. (D) miRNA in situ hybridization assay demonstrated the PGC-enriched expression patterns of miRNAs in PGCs at 12.5 dpc. Arrow, a seminiferous cord; black arrowhead, primordial germ cells; white arrowhead, Sertoli cells.
    Figure Legend Snippet: The expression levels of miRNAs during male germ cell development. (A) Expression profiling of 12 PGC-enriched miRNAs identified by miRNA microarray in developing male germ cells. For each miRNA, the mean-centered log 2 -expression profile (mean = 0) is shown. A hierarchical clustering was done to visualize the similarity in expression patterns of the miRNAs. The colors represent increased (red) and decreased (green) expression relative to the mean expression level. (B) PGC-enriched expression of miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301 (red) among 12 miRNAs identified from a miRNA microarray analysis were confirmed by miRNA qPCR. (C) Northern blot analysis demonstrates that miR-18a, -93, -106b, and -181a were expressed in PGCs during male germ cell development. In the bottom, Ethidium bromide staining of 28S, 18S, and 5S rRNAs is shown as a loading control. Northern blot assay was performed as described in Materials and Methods. (D) miRNA in situ hybridization assay demonstrated the PGC-enriched expression patterns of miRNAs in PGCs at 12.5 dpc. Arrow, a seminiferous cord; black arrowhead, primordial germ cells; white arrowhead, Sertoli cells.

    Techniques Used: Expressing, Microarray, Northern Blot, Staining, Control, In Situ Hybridization

    Identification of target genes by PGC-enriched miRNAs and their biological processes. (A) The expression of target genes categorized in C1 and C2 showed a reverse correlation with nine PGC-enriched miRNAs during germ cell development. The auto-scaled log 2 -expression profiles of each target gene (mean = 0 and standard deviation = 1) are shown (gray line). Median expression values in C1 and C2 for each sample are expressed as blue and red lines. (B) The strategy for identifying target genes by nine PGC-enriched miRNAs. 6,511 targets were generated from the three databases using the sequence pairing algorithms. Among them, 127 were selected as target genes having a reverse expression (C1 and C2) with miRNA expression. (C) A heat map showed expression patterns of the 127 target genes. For each gene, the mean-centered log 2 -expression profile (mean = 0) is shown. A hierarchical clustering was done to visualize the similarity in expression patterns of the genes. The colors represent increased (red) and decreased (green) expression relative to the mean expression level. (D) miR-106b and -93 shared target genes due to possession of similar seed sequence. Similarly, miR-181a and -181b shared target genes.
    Figure Legend Snippet: Identification of target genes by PGC-enriched miRNAs and their biological processes. (A) The expression of target genes categorized in C1 and C2 showed a reverse correlation with nine PGC-enriched miRNAs during germ cell development. The auto-scaled log 2 -expression profiles of each target gene (mean = 0 and standard deviation = 1) are shown (gray line). Median expression values in C1 and C2 for each sample are expressed as blue and red lines. (B) The strategy for identifying target genes by nine PGC-enriched miRNAs. 6,511 targets were generated from the three databases using the sequence pairing algorithms. Among them, 127 were selected as target genes having a reverse expression (C1 and C2) with miRNA expression. (C) A heat map showed expression patterns of the 127 target genes. For each gene, the mean-centered log 2 -expression profile (mean = 0) is shown. A hierarchical clustering was done to visualize the similarity in expression patterns of the genes. The colors represent increased (red) and decreased (green) expression relative to the mean expression level. (D) miR-106b and -93 shared target genes due to possession of similar seed sequence. Similarly, miR-181a and -181b shared target genes.

    Techniques Used: Expressing, Standard Deviation, Generated, Sequencing

    A network model delineates biological processes regulated by PGC-enriched miRNAs. (A) The enrichment analysis results of GOBPs by total genes in C1 and C2 (red bar, total genes) and 127 target genes (blue bar). The red line denotes the cutoff value (P-value = 0.1) of the analysis. (B) A network model shows the relationships between nine PGC-enriched miRNAs and their target genes. Colors and shapes of the nodes represent PGC-enriched miRNAs (green diamonds), target genes in C1 (purple circles) and C2 (red circles). Edge colors were used to distinguish the interactions of individual miRNAs or miRNA families. The nodes were grouped according to their gene ontologies (colored rectangles). Nodes containing the same gene ontologies belong to same group as described in “Materials and Methods”. The genes with multiple gene ontologies were comprised of two or more functional groups. The background colors represent gene ontology groups (ellipsoidal for lower-level gene ontologies and rectangle for upper-level gene ontologies).
    Figure Legend Snippet: A network model delineates biological processes regulated by PGC-enriched miRNAs. (A) The enrichment analysis results of GOBPs by total genes in C1 and C2 (red bar, total genes) and 127 target genes (blue bar). The red line denotes the cutoff value (P-value = 0.1) of the analysis. (B) A network model shows the relationships between nine PGC-enriched miRNAs and their target genes. Colors and shapes of the nodes represent PGC-enriched miRNAs (green diamonds), target genes in C1 (purple circles) and C2 (red circles). Edge colors were used to distinguish the interactions of individual miRNAs or miRNA families. The nodes were grouped according to their gene ontologies (colored rectangles). Nodes containing the same gene ontologies belong to same group as described in “Materials and Methods”. The genes with multiple gene ontologies were comprised of two or more functional groups. The background colors represent gene ontology groups (ellipsoidal for lower-level gene ontologies and rectangle for upper-level gene ontologies).

    Techniques Used: Functional Assay

    A heat map represents the relationship between each PGC-enriched miRNA and the twelve biological processes. (A) A heat map shows the contribution score defined by the number of target genes regulated by each miRNA within a gene ontology group divided by the total number of the target genes of the miRNA within the group. (B) RT-PCR analysis confirms expression of the representative target genes during male germ cell development.
    Figure Legend Snippet: A heat map represents the relationship between each PGC-enriched miRNA and the twelve biological processes. (A) A heat map shows the contribution score defined by the number of target genes regulated by each miRNA within a gene ontology group divided by the total number of the target genes of the miRNA within the group. (B) RT-PCR analysis confirms expression of the representative target genes during male germ cell development.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing

    Related Articles

    Microarray:

    Article Title: Dynamic expression of specific miRNAs during erythroid differentiation of human embryonic stem cells.
    Article Snippet: .. miRNAs profiles in hESCs, mesenchymal stem cells (MSCs) and CB CD34+ cells were analyzed using the Genopal miRNA microarray system (Mitsubishi Rayon Co., Ltd., Yokohama Japan) (Lee et al., 2010). ..

    Article Title: Dynamic Expression of Specific miRNAs during Erythroid Differentiation of Human Embryonic Stem Cells
    Article Snippet: .. Microarray analysis miRNAs profiles in hESCs, mesenchymal stem cells (MSCs) and CB CD34+ cells were analyzed using the Genopal miRNA microarray system (Mitsubishi Rayon Co., Ltd., Yokohama Japan) ( Lee et al., 2010 ). ..

    Article Title: Inhibitory effect of strawberry geranium (Saxifraga stolonifera) on Toll-like receptor 2-mediated inflammatory response in human skin keratinocytes.
    Article Snippet: Ethnopharmacological relevance: Strawberry geranium (Saxifraga stolonifera [L.] Meeb) has traditionally been used as a drug to treat skin disorders in Japan.. However, little is known about its physiological effects on skin keratinocytes.. Aim of the study: We investigated the anti-inflammatory effects of a strawberry geranium extract (SGE) on human skin keratinocytes.

    Article Title: Dynamic expression of specific miRNAs during erythroid differentiation of human embryonic stem cells.
    Article Snippet: .. We are grateful to Mitsubishi Rayon Co., Ltd. for providing the Genopal microarray system and for excellent technical assistance. .. This work was supported part by Stem Cell Research Center (SC-2220) and Medical Research Center (2011- 0028263) program funded by Ministry of Education, Science and Technology, Republic of Korea (to K.S.K.).

    Article Title: Functional Characterization of Domains of IPS-1 Using an Inducible Oligomerization System
    Article Snippet: .. In the microarray analysis, we used the Genopal microarray system according to the manufacturer’s instructions (Mitsubishi Rayon). .. Biotin-labeled RNA was prepared with a MessageAmp II-Biotin Enhanced kit (Ambion).

    Article Title: Virus-Infection or 5′ppp-RNA Activates Antiviral Signal through Redistribution of IPS-1 Mediated by MFN1
    Article Snippet: The siRNA negative control and siRNAs targeting MFN1, OPA1, or DRP1 were purchased from Invitrogen, and transfected with RNAi MAX (Invitrogen) according to the manufacturer's recommendation (final concentration of siRNA was 50nM). .. In the microarray analysis, we used the Genopal microarray system according to the manufacturer's instructions (Mitsubishi Rayon). .. Total RNA was prepared with TRIZOL (Invitrogen) and treated with DNase I (Roche Diagnostics).

    other:

    Article Title: Dynamic Expression of Specific miRNAs during Erythroid Differentiation of Human Embryonic Stem Cells
    Article Snippet: In addition, cell morphology was examined with Wright-Giemsa staining under digital camera (Leica, Germany) at 1,000 fold magnification.

    Article Title: PGC-Enriched miRNAs Control Germ Cell Development
    Article Snippet: A hybridization assay was carried out with Genopal-MICM miRNA arrays (Mitsubishi Rayon, Japan) overnight, where 180-oligonucleotide DNA probes are installed for detection of mouse miRNAs in 150 μl of hybridization buffer containing 2x SSC, 0.2% SDS, and ∼1 μg of heat-denatured labeled RNAs at 50°C.

    Analysis:

    Article Title: Functional Characterization of Domains of IPS-1 Using an Inducible Oligomerization System
    Article Snippet: .. In the microarray analysis, we used the Genopal microarray system according to the manufacturer’s instructions (Mitsubishi Rayon). .. Biotin-labeled RNA was prepared with a MessageAmp II-Biotin Enhanced kit (Ambion).



    Similar Products

    dna microarray genopal mouse custom chip  (Mitsubishi Rayon CO)
    90
    Mitsubishi Rayon CO dna microarray genopal mouse custom chip
    Dna Microarray Genopal Mouse Custom Chip, supplied by Mitsubishi Rayon CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+genopal+mouse+custom+chip/dna+microarray+genopal/pm25445590-72-2-10
    Average 90 stars, based on 1 article reviews
    dna microarray genopal mouse custom chip - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results