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truseq short insert libraries  (Illumina Inc)


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    Illumina Inc truseq short insert libraries
    Truseq Short Insert Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 13185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+insert/TruSeq+DNA+Library+Prep/pm41887342-87-6-12
    Average 99 stars, based on 13185 article reviews
    truseq short insert libraries - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Sample Prep:

    Article Title: Exploratory Shotgun Metagenomic Analysis of the Fecal Microbiome and Antimicrobial-Resistance Potential in Simmental Calves with Different Diarrheal Statuses
    Article Snippet: .. stool DNA Kit (D401500S) (Omega Bio-Tek, Norcross, GA, USA); TruSeq DNA Sample Prep Kit (Illumina, San Diego, CA, USA); phosphate buffered saline (HyClone, Logan, UT, USA); Covaris M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). ..

    Article Title: Plant growth improvement and microbial community reorganization are decoupled during restoration of trampled urban forest soil.
    Article Snippet: PCR used TransStart FastPfu DNA Polymerase (TransGen AP221-02) on an ABI GeneAmp 9700 thermocycler, and three replicate reactions per sample were pooled. .. Amplicons were separated on 2% agarose gels, purified with the AxyPrep DNA Gel Extraction Kit (Axygen, Union City, CA, USA), quantified with the QuantiFluor-ST system (Promega), pooled at equimolar concentrations, and prepared with a TruSeq DNA Sample Prep Kit for paired-end sequencing on an Illumina NextSeq 2000 platform. ..

    Article Title: Probiotic and safety characterization of Lactiplantibacillus plantarum ZZM01 isolated from mink.
    Article Snippet: .. A paired-end library was constructed using the TruSeq DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina NovaSeq platform to obtain high-quality short reads. .. A long-read library was prepared according to the Oxford Nanopore Technologies protocol and sequenced on the PromethION platform (Oxford Nanopore Technologies, Oxford, UK).

    Article Title: Stepwise evolution of tigecycline resistance in Enterococcus hirae through early IS1216E-mediated tet(L) amplification and subsequent rpsJ mutations.
    Article Snippet: Genomic DNA was extracted using the E.Z.N.A. Bacterial DNA Kit (Omega Bio-tek, USA) according to the manufacturer’s instructions. .. Illumina sequencing libraries were prepared using the TruSeq DNA Sample Prep Kit (Illumina, USA) following the manufacturer’s protocol. .. Short-read sequencing (2 × 150 bp) was performed on the Illumina NovaSeq platform, and long-read sequencing was conducted using the Oxford Nanopore Technologies MinION platform.

    Article Title: Identification and whole-genome sequencing of blood-brain barrier-penetrating Riemerella anatipestifer isolated from chickens and ducks.
    Article Snippet: DNA concentration, quality, and integrity were determined using a Qubit Fluorometer (Invitrogen, USA) and a NanoDrop Spectrophotometer (Thermo Scientific, USA). .. Sequencing libraries were generated using the TruSeq DNA Sample Preparation Kit (Illumina, USA) and the Template Prep Kit (Pacific Biosciences, USA). .. Genome sequencing was performed by Personal Biotechnology Co., Ltd. (Shanghai, China) using the Nanopore PromethION 48 platform and the Illumina NovaSeq platform.

    Saline:

    Article Title: Exploratory Shotgun Metagenomic Analysis of the Fecal Microbiome and Antimicrobial-Resistance Potential in Simmental Calves with Different Diarrheal Statuses
    Article Snippet: .. stool DNA Kit (D401500S) (Omega Bio-Tek, Norcross, GA, USA); TruSeq DNA Sample Prep Kit (Illumina, San Diego, CA, USA); phosphate buffered saline (HyClone, Logan, UT, USA); Covaris M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). ..

    Sequencing:

    Article Title: Effects of dietary barley malt extract on growth performance, immune parameters, intestinal fermentation and gut microbiota in nursery piglets.
    Article Snippet: .. Samples were pooled in equimolar concentrations and prepared for sequencing using the Illumina TruSeq DNA Library Preparation Kit (Illumina, San Diego, CA, USA). .. Sequencing was performed on an Illumina MiSeq platform (2 × 250-bp paired end reads) according to the manufacturer’s instructions.

    Article Title: Plant growth improvement and microbial community reorganization are decoupled during restoration of trampled urban forest soil.
    Article Snippet: PCR used TransStart FastPfu DNA Polymerase (TransGen AP221-02) on an ABI GeneAmp 9700 thermocycler, and three replicate reactions per sample were pooled. .. Amplicons were separated on 2% agarose gels, purified with the AxyPrep DNA Gel Extraction Kit (Axygen, Union City, CA, USA), quantified with the QuantiFluor-ST system (Promega), pooled at equimolar concentrations, and prepared with a TruSeq DNA Sample Prep Kit for paired-end sequencing on an Illumina NextSeq 2000 platform. ..

    Article Title: Recent geographic dispersal and environmental adaptation of indica rice.
    Article Snippet: 6 Genomic DNA was extracted from the seedlings using Qiagen Plant DNEasy kits according to 7 manufacturer’s protocols. .. Extracted DNA from each sample was prepared for Illumina genome 8 sequencing using either the Illumina TruSeq or Nextera DNA Library Preparation Kit. .. 9 Sequencing was done on the Illumina NovaSeq S2 with 2x100 bp read configuration.

    Article Title: Identification and whole-genome sequencing of blood-brain barrier-penetrating Riemerella anatipestifer isolated from chickens and ducks.
    Article Snippet: DNA concentration, quality, and integrity were determined using a Qubit Fluorometer (Invitrogen, USA) and a NanoDrop Spectrophotometer (Thermo Scientific, USA). .. Sequencing libraries were generated using the TruSeq DNA Sample Preparation Kit (Illumina, USA) and the Template Prep Kit (Pacific Biosciences, USA). .. Genome sequencing was performed by Personal Biotechnology Co., Ltd. (Shanghai, China) using the Nanopore PromethION 48 platform and the Illumina NovaSeq platform.

    DNA Library Preparation:

    Article Title: Effects of dietary barley malt extract on growth performance, immune parameters, intestinal fermentation and gut microbiota in nursery piglets.
    Article Snippet: .. Samples were pooled in equimolar concentrations and prepared for sequencing using the Illumina TruSeq DNA Library Preparation Kit (Illumina, San Diego, CA, USA). .. Sequencing was performed on an Illumina MiSeq platform (2 × 250-bp paired end reads) according to the manufacturer’s instructions.

    Article Title: Recent geographic dispersal and environmental adaptation of indica rice.
    Article Snippet: 6 Genomic DNA was extracted from the seedlings using Qiagen Plant DNEasy kits according to 7 manufacturer’s protocols. .. Extracted DNA from each sample was prepared for Illumina genome 8 sequencing using either the Illumina TruSeq or Nextera DNA Library Preparation Kit. .. 9 Sequencing was done on the Illumina NovaSeq S2 with 2x100 bp read configuration.

    Purification:

    Article Title: Plant growth improvement and microbial community reorganization are decoupled during restoration of trampled urban forest soil.
    Article Snippet: PCR used TransStart FastPfu DNA Polymerase (TransGen AP221-02) on an ABI GeneAmp 9700 thermocycler, and three replicate reactions per sample were pooled. .. Amplicons were separated on 2% agarose gels, purified with the AxyPrep DNA Gel Extraction Kit (Axygen, Union City, CA, USA), quantified with the QuantiFluor-ST system (Promega), pooled at equimolar concentrations, and prepared with a TruSeq DNA Sample Prep Kit for paired-end sequencing on an Illumina NextSeq 2000 platform. ..

    Gel Extraction:

    Article Title: Plant growth improvement and microbial community reorganization are decoupled during restoration of trampled urban forest soil.
    Article Snippet: PCR used TransStart FastPfu DNA Polymerase (TransGen AP221-02) on an ABI GeneAmp 9700 thermocycler, and three replicate reactions per sample were pooled. .. Amplicons were separated on 2% agarose gels, purified with the AxyPrep DNA Gel Extraction Kit (Axygen, Union City, CA, USA), quantified with the QuantiFluor-ST system (Promega), pooled at equimolar concentrations, and prepared with a TruSeq DNA Sample Prep Kit for paired-end sequencing on an Illumina NextSeq 2000 platform. ..

    Construct:

    Article Title: Probiotic and safety characterization of Lactiplantibacillus plantarum ZZM01 isolated from mink.
    Article Snippet: .. A paired-end library was constructed using the TruSeq DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina NovaSeq platform to obtain high-quality short reads. .. A long-read library was prepared according to the Oxford Nanopore Technologies protocol and sequenced on the PromethION platform (Oxford Nanopore Technologies, Oxford, UK).

    Article Title: Bistability of rhizosphere microbiomes shapes phosphorus partitioning in river networks under water diversion.
    Article Snippet: Inter-basin water transfer projects are critical for mitigating water scarcity yet impose complex environmental disturbances, altering phosphorus (P) partitioning in recipient river networks.. In these ecosystems, rhizosphere microbial communities (RMCs) act as biogeochemical engines regulating P partitioning among water, sediment, and biological phases.. This regulatory capacity relies on structural and functional traits of RMC states.

    Generated:

    Article Title: Identification and whole-genome sequencing of blood-brain barrier-penetrating Riemerella anatipestifer isolated from chickens and ducks.
    Article Snippet: DNA concentration, quality, and integrity were determined using a Qubit Fluorometer (Invitrogen, USA) and a NanoDrop Spectrophotometer (Thermo Scientific, USA). .. Sequencing libraries were generated using the TruSeq DNA Sample Preparation Kit (Illumina, USA) and the Template Prep Kit (Pacific Biosciences, USA). .. Genome sequencing was performed by Personal Biotechnology Co., Ltd. (Shanghai, China) using the Nanopore PromethION 48 platform and the Illumina NovaSeq platform.



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    (A) Cartoon representation of the experimental strategy used in this study. Libraries were generated from a pooled oligo library containing fragments coding for each type of single-residue missense variant within positions 927-974 of the <t>rat</t> <t>TRPM8</t> channel. Oligo fragments were PCR-amplified and cloned into ‘acceptor’ expression plasmids by Golden Gate cloning using BsmBI sites . (B) The plasmid library was expressed in iCasp9 cells , and cells expressing mCherry were sorted by flow cytometry into groups of low, medium, or high GCaMP6s fluorescence intensity after stimulation with cold or 2 mM menthol. The genomic <t>DNA</t> was extracted from the sorted cells, from which the TRPM8 coding region was amplified by PCR, cleaved by a restriction endonuclease to reduce amplicon size, and prepared for next-generation sequencing. (C) NGS read counts per variant in the plasmid library, showing homogenous variant representation. (D) mCherry fluorescence intensity distribution in the library cells that were sorted. (E) GCaMP6s fluorescence intensity distribution of the library cells, measured at room temperature (dashed black curves, not sorted), or after stimulation with cold (blue) or 2 mM menthol (green) during cell sorting. Dashed red lines denote the three cell-sorting bins. (F) Deep mutational scanning score distribution for cold and menthol experiments. (G) Correlation between individual variant scores from two biological replicates. (H) GCaMP6s fluorescence responses in four silent mutations included in the library (gray traces) relative to cells expressing WT.
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    Image Search Results


    (A) Cartoon representation of the experimental strategy used in this study. Libraries were generated from a pooled oligo library containing fragments coding for each type of single-residue missense variant within positions 927-974 of the rat TRPM8 channel. Oligo fragments were PCR-amplified and cloned into ‘acceptor’ expression plasmids by Golden Gate cloning using BsmBI sites . (B) The plasmid library was expressed in iCasp9 cells , and cells expressing mCherry were sorted by flow cytometry into groups of low, medium, or high GCaMP6s fluorescence intensity after stimulation with cold or 2 mM menthol. The genomic DNA was extracted from the sorted cells, from which the TRPM8 coding region was amplified by PCR, cleaved by a restriction endonuclease to reduce amplicon size, and prepared for next-generation sequencing. (C) NGS read counts per variant in the plasmid library, showing homogenous variant representation. (D) mCherry fluorescence intensity distribution in the library cells that were sorted. (E) GCaMP6s fluorescence intensity distribution of the library cells, measured at room temperature (dashed black curves, not sorted), or after stimulation with cold (blue) or 2 mM menthol (green) during cell sorting. Dashed red lines denote the three cell-sorting bins. (F) Deep mutational scanning score distribution for cold and menthol experiments. (G) Correlation between individual variant scores from two biological replicates. (H) GCaMP6s fluorescence responses in four silent mutations included in the library (gray traces) relative to cells expressing WT.

    Journal: bioRxiv

    Article Title: Deep mutational scan of the pore of the cold-sensing TRPM8 channel

    doi: 10.64898/2026.04.28.721489

    Figure Lengend Snippet: (A) Cartoon representation of the experimental strategy used in this study. Libraries were generated from a pooled oligo library containing fragments coding for each type of single-residue missense variant within positions 927-974 of the rat TRPM8 channel. Oligo fragments were PCR-amplified and cloned into ‘acceptor’ expression plasmids by Golden Gate cloning using BsmBI sites . (B) The plasmid library was expressed in iCasp9 cells , and cells expressing mCherry were sorted by flow cytometry into groups of low, medium, or high GCaMP6s fluorescence intensity after stimulation with cold or 2 mM menthol. The genomic DNA was extracted from the sorted cells, from which the TRPM8 coding region was amplified by PCR, cleaved by a restriction endonuclease to reduce amplicon size, and prepared for next-generation sequencing. (C) NGS read counts per variant in the plasmid library, showing homogenous variant representation. (D) mCherry fluorescence intensity distribution in the library cells that were sorted. (E) GCaMP6s fluorescence intensity distribution of the library cells, measured at room temperature (dashed black curves, not sorted), or after stimulation with cold (blue) or 2 mM menthol (green) during cell sorting. Dashed red lines denote the three cell-sorting bins. (F) Deep mutational scanning score distribution for cold and menthol experiments. (G) Correlation between individual variant scores from two biological replicates. (H) GCaMP6s fluorescence responses in four silent mutations included in the library (gray traces) relative to cells expressing WT.

    Article Snippet: To generate the acceptor plasmid for the library, we used restriction sites MfeI and SphI, which flank the sequence coding for the transmembrane domain of TRPM8, to clone a double stranded DNA insert (Twist Bioscience) that included BsmBI sites flanking the variable-sequence region of the library from positions 927 to 974.

    Techniques: Generated, Residue, Variant Assay, Amplification, Clone Assay, Expressing, Cloning, Plasmid Preparation, Flow Cytometry, Fluorescence, Next-Generation Sequencing, FACS