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DuPont de Nemours 3h-thymidine
3h Thymidine, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+depolymerization/3h+thymidine/pm10622265-56-14-19
Average 90 stars, based on 1 article reviews
3h-thymidine - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Mouse Assay:

Article Title: Compositions, kits, and methods for the diagnosis, prognosis, monitoring, treatment and modulation of post-transplant lymphoproliferative disorders and hypoxia associated angiogenesis disorders using galectin-1
Article Snippet: Q. Intracellular Staining and FACS Analysis For intracellular cytokine staining, TLDN or tumor-infiltrating lymphocytes were made permeable with Perm2 solution (BD Biosciences) and were labeled with fluorescent-labeled monoclonal anti-IFN-γ (XMG1.2; BD Biosciences), anti-IL-17 (TC11-18H10; BD Biosciences), anti-IL-10 (JES5-16E3; eBioscience), anti-CD4 (GK1.5; BD Biosciences), anti-CD8 (H35-17.2; eBioscience) antibodies.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: PHA blasts (activated T cells) are generated by resuspending cells in RPMI 1640 containing 10% fetal bovine serum (Hyclone), to which 1% (w/v) of each of the following is added: L-glutamine; non-essential amino acids; sodium pyruvate; and antibiotic-antimycotic (RPMI media) at a density of 106 cells/ml.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure.
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO2 inhalation after five hours.

Article Title: Components of the Glutathione Cycle as Markers of Biological Age: An Approach to Clinical Application in Aging.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3Htimidine was added (Dupont, Boston, MA, USA), followed by another 24 h of incubation.

Article Title: Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3H-thymidine (Dupont, Boston, MA, USA) was added, followed by an additional 24 h incubation.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO 2 inhalation after five hours.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: Cells are harvested and washed two times in DPBS, resuspended in RPMI media and plated into 96-well flat bottom plates at a density of 105 cells/well in 200 μl with varying concentrations of IL2 or immunocytokine variant in RPMI media Plates were incubated for 48 hours at 37 C., pulsed with 1 μCi 3H-thymidine (DuPont NEN®, Boston, Mass.)/well for 6 hours, harvested, and radioactivity is measured after harvesting cells onto glass fiber filters.

Article Title: The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats.
Article Snippet: Platelet-derived growth factor BB (BB) regulates cell proliferation and function.. However, the roles of BB on proliferation and function of Leydig stem (LSCs) and progenitor cells (LPCs) and the underlying signaling pathways remain unclear.. This study aimed to analyze the roles of PI3K and MAPK pathways in the regulation of proliferation-related and steroidogenesis-related gene expression in rat LSCs/LPCs.

Injection:

Article Title: Compositions, kits, and methods for the diagnosis, prognosis, monitoring, treatment and modulation of post-transplant lymphoproliferative disorders and hypoxia associated angiogenesis disorders using galectin-1
Article Snippet: Q. Intracellular Staining and FACS Analysis For intracellular cytokine staining, TLDN or tumor-infiltrating lymphocytes were made permeable with Perm2 solution (BD Biosciences) and were labeled with fluorescent-labeled monoclonal anti-IFN-γ (XMG1.2; BD Biosciences), anti-IL-17 (TC11-18H10; BD Biosciences), anti-IL-10 (JES5-16E3; eBioscience), anti-CD4 (GK1.5; BD Biosciences), anti-CD8 (H35-17.2; eBioscience) antibodies.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: PHA blasts (activated T cells) are generated by resuspending cells in RPMI 1640 containing 10% fetal bovine serum (Hyclone), to which 1% (w/v) of each of the following is added: L-glutamine; non-essential amino acids; sodium pyruvate; and antibiotic-antimycotic (RPMI media) at a density of 106 cells/ml.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure.
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO2 inhalation after five hours.

Article Title: Components of the Glutathione Cycle as Markers of Biological Age: An Approach to Clinical Application in Aging.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3Htimidine was added (Dupont, Boston, MA, USA), followed by another 24 h of incubation.

Article Title: Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3H-thymidine (Dupont, Boston, MA, USA) was added, followed by an additional 24 h incubation.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO 2 inhalation after five hours.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: Cells are harvested and washed two times in DPBS, resuspended in RPMI media and plated into 96-well flat bottom plates at a density of 105 cells/well in 200 μl with varying concentrations of IL2 or immunocytokine variant in RPMI media Plates were incubated for 48 hours at 37 C., pulsed with 1 μCi 3H-thymidine (DuPont NEN®, Boston, Mass.)/well for 6 hours, harvested, and radioactivity is measured after harvesting cells onto glass fiber filters.

Article Title: The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats.
Article Snippet: Platelet-derived growth factor BB (BB) regulates cell proliferation and function.. However, the roles of BB on proliferation and function of Leydig stem (LSCs) and progenitor cells (LPCs) and the underlying signaling pathways remain unclear.. This study aimed to analyze the roles of PI3K and MAPK pathways in the regulation of proliferation-related and steroidogenesis-related gene expression in rat LSCs/LPCs.

Activity Assay:

Article Title: Compositions, kits, and methods for the diagnosis, prognosis, monitoring, treatment and modulation of post-transplant lymphoproliferative disorders and hypoxia associated angiogenesis disorders using galectin-1
Article Snippet: Q. Intracellular Staining and FACS Analysis For intracellular cytokine staining, TLDN or tumor-infiltrating lymphocytes were made permeable with Perm2 solution (BD Biosciences) and were labeled with fluorescent-labeled monoclonal anti-IFN-γ (XMG1.2; BD Biosciences), anti-IL-17 (TC11-18H10; BD Biosciences), anti-IL-10 (JES5-16E3; eBioscience), anti-CD4 (GK1.5; BD Biosciences), anti-CD8 (H35-17.2; eBioscience) antibodies.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: PHA blasts (activated T cells) are generated by resuspending cells in RPMI 1640 containing 10% fetal bovine serum (Hyclone), to which 1% (w/v) of each of the following is added: L-glutamine; non-essential amino acids; sodium pyruvate; and antibiotic-antimycotic (RPMI media) at a density of 106 cells/ml.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure.
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO2 inhalation after five hours.

Article Title: Components of the Glutathione Cycle as Markers of Biological Age: An Approach to Clinical Application in Aging.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3Htimidine was added (Dupont, Boston, MA, USA), followed by another 24 h of incubation.

Article Title: Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3H-thymidine (Dupont, Boston, MA, USA) was added, followed by an additional 24 h incubation.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO 2 inhalation after five hours.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: Cells are harvested and washed two times in DPBS, resuspended in RPMI media and plated into 96-well flat bottom plates at a density of 105 cells/well in 200 μl with varying concentrations of IL2 or immunocytokine variant in RPMI media Plates were incubated for 48 hours at 37 C., pulsed with 1 μCi 3H-thymidine (DuPont NEN®, Boston, Mass.)/well for 6 hours, harvested, and radioactivity is measured after harvesting cells onto glass fiber filters.

Article Title: The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats.
Article Snippet: Platelet-derived growth factor BB (BB) regulates cell proliferation and function.. However, the roles of BB on proliferation and function of Leydig stem (LSCs) and progenitor cells (LPCs) and the underlying signaling pathways remain unclear.. This study aimed to analyze the roles of PI3K and MAPK pathways in the regulation of proliferation-related and steroidogenesis-related gene expression in rat LSCs/LPCs.

Incubation:

Article Title: Compositions, kits, and methods for the diagnosis, prognosis, monitoring, treatment and modulation of post-transplant lymphoproliferative disorders and hypoxia associated angiogenesis disorders using galectin-1
Article Snippet: Q. Intracellular Staining and FACS Analysis For intracellular cytokine staining, TLDN or tumor-infiltrating lymphocytes were made permeable with Perm2 solution (BD Biosciences) and were labeled with fluorescent-labeled monoclonal anti-IFN-γ (XMG1.2; BD Biosciences), anti-IL-17 (TC11-18H10; BD Biosciences), anti-IL-10 (JES5-16E3; eBioscience), anti-CD4 (GK1.5; BD Biosciences), anti-CD8 (H35-17.2; eBioscience) antibodies.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: PHA blasts (activated T cells) are generated by resuspending cells in RPMI 1640 containing 10% fetal bovine serum (Hyclone), to which 1% (w/v) of each of the following is added: L-glutamine; non-essential amino acids; sodium pyruvate; and antibiotic-antimycotic (RPMI media) at a density of 106 cells/ml.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure.
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO2 inhalation after five hours.

Article Title: Components of the Glutathione Cycle as Markers of Biological Age: An Approach to Clinical Application in Aging.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3Htimidine was added (Dupont, Boston, MA, USA), followed by another 24 h of incubation.

Article Title: Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3H-thymidine (Dupont, Boston, MA, USA) was added, followed by an additional 24 h incubation.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO 2 inhalation after five hours.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: Cells are harvested and washed two times in DPBS, resuspended in RPMI media and plated into 96-well flat bottom plates at a density of 105 cells/well in 200 μl with varying concentrations of IL2 or immunocytokine variant in RPMI media Plates were incubated for 48 hours at 37 C., pulsed with 1 μCi 3H-thymidine (DuPont NEN®, Boston, Mass.)/well for 6 hours, harvested, and radioactivity is measured after harvesting cells onto glass fiber filters.

Article Title: The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats.
Article Snippet: Platelet-derived growth factor BB (BB) regulates cell proliferation and function.. However, the roles of BB on proliferation and function of Leydig stem (LSCs) and progenitor cells (LPCs) and the underlying signaling pathways remain unclear.. This study aimed to analyze the roles of PI3K and MAPK pathways in the regulation of proliferation-related and steroidogenesis-related gene expression in rat LSCs/LPCs.

Radioactivity:

Article Title: Compositions, kits, and methods for the diagnosis, prognosis, monitoring, treatment and modulation of post-transplant lymphoproliferative disorders and hypoxia associated angiogenesis disorders using galectin-1
Article Snippet: Q. Intracellular Staining and FACS Analysis For intracellular cytokine staining, TLDN or tumor-infiltrating lymphocytes were made permeable with Perm2 solution (BD Biosciences) and were labeled with fluorescent-labeled monoclonal anti-IFN-γ (XMG1.2; BD Biosciences), anti-IL-17 (TC11-18H10; BD Biosciences), anti-IL-10 (JES5-16E3; eBioscience), anti-CD4 (GK1.5; BD Biosciences), anti-CD8 (H35-17.2; eBioscience) antibodies.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: PHA blasts (activated T cells) are generated by resuspending cells in RPMI 1640 containing 10% fetal bovine serum (Hyclone), to which 1% (w/v) of each of the following is added: L-glutamine; non-essential amino acids; sodium pyruvate; and antibiotic-antimycotic (RPMI media) at a density of 106 cells/ml.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure.
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO2 inhalation after five hours.

Article Title: Components of the Glutathione Cycle as Markers of Biological Age: An Approach to Clinical Application in Aging.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3Htimidine was added (Dupont, Boston, MA, USA), followed by another 24 h of incubation.

Article Title: Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3H-thymidine (Dupont, Boston, MA, USA) was added, followed by an additional 24 h incubation.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO 2 inhalation after five hours.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: Cells are harvested and washed two times in DPBS, resuspended in RPMI media and plated into 96-well flat bottom plates at a density of 105 cells/well in 200 μl with varying concentrations of IL2 or immunocytokine variant in RPMI media Plates were incubated for 48 hours at 37 C., pulsed with 1 μCi 3H-thymidine (DuPont NEN®, Boston, Mass.)/well for 6 hours, harvested, and radioactivity is measured after harvesting cells onto glass fiber filters.

Article Title: The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats.
Article Snippet: Platelet-derived growth factor BB (BB) regulates cell proliferation and function.. However, the roles of BB on proliferation and function of Leydig stem (LSCs) and progenitor cells (LPCs) and the underlying signaling pathways remain unclear.. This study aimed to analyze the roles of PI3K and MAPK pathways in the regulation of proliferation-related and steroidogenesis-related gene expression in rat LSCs/LPCs.

Variant Assay:

Article Title: Compositions, kits, and methods for the diagnosis, prognosis, monitoring, treatment and modulation of post-transplant lymphoproliferative disorders and hypoxia associated angiogenesis disorders using galectin-1
Article Snippet: Q. Intracellular Staining and FACS Analysis For intracellular cytokine staining, TLDN or tumor-infiltrating lymphocytes were made permeable with Perm2 solution (BD Biosciences) and were labeled with fluorescent-labeled monoclonal anti-IFN-γ (XMG1.2; BD Biosciences), anti-IL-17 (TC11-18H10; BD Biosciences), anti-IL-10 (JES5-16E3; eBioscience), anti-CD4 (GK1.5; BD Biosciences), anti-CD8 (H35-17.2; eBioscience) antibodies.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: PHA blasts (activated T cells) are generated by resuspending cells in RPMI 1640 containing 10% fetal bovine serum (Hyclone), to which 1% (w/v) of each of the following is added: L-glutamine; non-essential amino acids; sodium pyruvate; and antibiotic-antimycotic (RPMI media) at a density of 106 cells/ml.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure.
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO2 inhalation after five hours.

Article Title: Components of the Glutathione Cycle as Markers of Biological Age: An Approach to Clinical Application in Aging.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3Htimidine was added (Dupont, Boston, MA, USA), followed by another 24 h of incubation.

Article Title: Supplementation with Bioactive Compounds Improves Health and Rejuvenates Biological Age in Postmenopausal Women.
Article Snippet: After 48 h of incubation, 0.5 μCi/well of 3H-thymidine (Dupont, Boston, MA, USA) was added, followed by an additional 24 h incubation.

Article Title: Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure
Article Snippet: Mice were then injected intravenously via the lateral tail vein with 20 μCi 3H-thymidine (Dupont NEN, Waltham, MA, USA; specific activity 2 Ci/mmol) 48 h following the final exposure, then euthanized via CO 2 inhalation after five hours.

Article Title: Light chain immunoglobulin fusion proteins and methods of use thereof
Article Snippet: Cells are harvested and washed two times in DPBS, resuspended in RPMI media and plated into 96-well flat bottom plates at a density of 105 cells/well in 200 μl with varying concentrations of IL2 or immunocytokine variant in RPMI media Plates were incubated for 48 hours at 37 C., pulsed with 1 μCi 3H-thymidine (DuPont NEN®, Boston, Mass.)/well for 6 hours, harvested, and radioactivity is measured after harvesting cells onto glass fiber filters.

Article Title: The role of platelet-derived growth factor BB signaling pathway in the regulation of stem and progenitor Leydig cell proliferation and steroidogenesis in male rats.
Article Snippet: Platelet-derived growth factor BB (BB) regulates cell proliferation and function.. However, the roles of BB on proliferation and function of Leydig stem (LSCs) and progenitor cells (LPCs) and the underlying signaling pathways remain unclear.. This study aimed to analyze the roles of PI3K and MAPK pathways in the regulation of proliferation-related and steroidogenesis-related gene expression in rat LSCs/LPCs.



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Schematic diagram of the formation of liquid-crystalline dispersion as a result of phase exclusion of linear ds <t>DNA</t> <t>molecules</t> <t>[molecular</t> mass (6–8) × 105 Da)] from PEG-containing water-salt solutions. 1. Initial ds DNA molecules in a PEG-containing solution (PEG molecules are shown by filled red circles) at CPEG below the critical value. 2. Particle of the DNA liquid-crystalline dispersion (encircled by an oval) formed at CPEG above the critical value. (P is the pitch of the helical twist of the spatial structure of a dispersion particle.) 3. A quasinematic layer formed by ds DNA molecules (the cross at the center denotes the rotation axis of the helical structure). [Note that DNA molecules in a quasinematic layer are located at a distance, which changes from 2.5 to 5.0 nm depending on the osmotic pressure of the solution (thick red frame and dark arrows are symbols of osmotic pressure of a PEG-containing solution)]
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Image Search Results


Schematic diagram of the formation of liquid-crystalline dispersion as a result of phase exclusion of linear ds DNA molecules [molecular mass (6–8) × 105 Da)] from PEG-containing water-salt solutions. 1. Initial ds DNA molecules in a PEG-containing solution (PEG molecules are shown by filled red circles) at CPEG below the critical value. 2. Particle of the DNA liquid-crystalline dispersion (encircled by an oval) formed at CPEG above the critical value. (P is the pitch of the helical twist of the spatial structure of a dispersion particle.) 3. A quasinematic layer formed by ds DNA molecules (the cross at the center denotes the rotation axis of the helical structure). [Note that DNA molecules in a quasinematic layer are located at a distance, which changes from 2.5 to 5.0 nm depending on the osmotic pressure of the solution (thick red frame and dark arrows are symbols of osmotic pressure of a PEG-containing solution)]

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: Schematic diagram of the formation of liquid-crystalline dispersion as a result of phase exclusion of linear ds DNA molecules [molecular mass (6–8) × 105 Da)] from PEG-containing water-salt solutions. 1. Initial ds DNA molecules in a PEG-containing solution (PEG molecules are shown by filled red circles) at CPEG below the critical value. 2. Particle of the DNA liquid-crystalline dispersion (encircled by an oval) formed at CPEG above the critical value. (P is the pitch of the helical twist of the spatial structure of a dispersion particle.) 3. A quasinematic layer formed by ds DNA molecules (the cross at the center denotes the rotation axis of the helical structure). [Note that DNA molecules in a quasinematic layer are located at a distance, which changes from 2.5 to 5.0 nm depending on the osmotic pressure of the solution (thick red frame and dark arrows are symbols of osmotic pressure of a PEG-containing solution)]

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques:

Experimental small-angle X-ray scattering curves from the phase formed via low-speed sedimentation of DNA LCD particles measured at four different temperatures. 1–20 °C, 2–40 °C, 3–60 °C, 4–80 °C. Conditions of DNA LCD particle formation: CDNA = 30 μg ml−1, CPEG = 300 mg ml−1, 0.3 M NaCl + 0.002 M Na+ phosphate buffer; T = 20 °C

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: Experimental small-angle X-ray scattering curves from the phase formed via low-speed sedimentation of DNA LCD particles measured at four different temperatures. 1–20 °C, 2–40 °C, 3–60 °C, 4–80 °C. Conditions of DNA LCD particle formation: CDNA = 30 μg ml−1, CPEG = 300 mg ml−1, 0.3 M NaCl + 0.002 M Na+ phosphate buffer; T = 20 °C

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques: Sedimentation

Experimental small-angle X-ray scattering curves from phases formed via low-speed sedimentation of DNA LCD particles. Conditions of DNA LCD particle formation: 1 - CPEG = 170 mg ml−1, 2 - CPEG = 300 mg ml−1. CDNA = 30 μg ml−1; 0.3 M NaCl + 0.002 M Na+ phosphate buffer. Inset: the dependence of the mean distance between DNA molecules (d) in liquid crystalline phases upon PEG concentration. a Experimental curves of small-angle X-ray scattering by liquid-crystalline phases formed via low-speed sedimentation of DNA LCD particles. b Fingerprint texture typical of the DNA classical cholesteric liquid-crystalline phase. CPEG = 170 mg ml−1; 0.3 M NaCl + 0.002 M Na+ phosphate buffer. Bar corresponds to 5 μm

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: Experimental small-angle X-ray scattering curves from phases formed via low-speed sedimentation of DNA LCD particles. Conditions of DNA LCD particle formation: 1 - CPEG = 170 mg ml−1, 2 - CPEG = 300 mg ml−1. CDNA = 30 μg ml−1; 0.3 M NaCl + 0.002 M Na+ phosphate buffer. Inset: the dependence of the mean distance between DNA molecules (d) in liquid crystalline phases upon PEG concentration. a Experimental curves of small-angle X-ray scattering by liquid-crystalline phases formed via low-speed sedimentation of DNA LCD particles. b Fingerprint texture typical of the DNA classical cholesteric liquid-crystalline phase. CPEG = 170 mg ml−1; 0.3 M NaCl + 0.002 M Na+ phosphate buffer. Bar corresponds to 5 μm

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques: Sedimentation, Concentration Assay

a Dependence of the amplitude of the abnormal band in the CD spectra of DNA LCDs (λ = 270 nm) formed in water-salt solutions (0.3 M NaCl + 0.002 M Na+ phosphate buffer) with different PEG concentrations on the temperature. 1 - CPEG = 220 mg ml−1, 2 - CPEG = 240 mg ml−1, 3 - CPEG = 260 mg ml−1, 4 - CPEG = 280 mg ml−1, 5 - CPEG = 300 mg ml−1. CDNA = 30 μg/ml. ΔA270 × 10−6 optical units; L = 1 cm. Arrows show the mean values of transition temperature (τtrans.) of DNA LCDs from the optically inactive to optically active state for two different condition sets. b Dependence of the value τtrans. versus PEG concentration. Different points relate to two series of independent experiments

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: a Dependence of the amplitude of the abnormal band in the CD spectra of DNA LCDs (λ = 270 nm) formed in water-salt solutions (0.3 M NaCl + 0.002 M Na+ phosphate buffer) with different PEG concentrations on the temperature. 1 - CPEG = 220 mg ml−1, 2 - CPEG = 240 mg ml−1, 3 - CPEG = 260 mg ml−1, 4 - CPEG = 280 mg ml−1, 5 - CPEG = 300 mg ml−1. CDNA = 30 μg/ml. ΔA270 × 10−6 optical units; L = 1 cm. Arrows show the mean values of transition temperature (τtrans.) of DNA LCDs from the optically inactive to optically active state for two different condition sets. b Dependence of the value τtrans. versus PEG concentration. Different points relate to two series of independent experiments

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques: Concentration Assay

Dependence of the amplitude of the abnormal band (λ = 270 nm) in the CD spectra of DNA LCDs formed at room temperature (curve 1), heated to 80 °C (curve 2) and cooled to room temperature (curve 3) on PEG concentration. CDNA = 30 μg ml−1, 0.3 M NaCl + 0.002 M Na+ phosphate buffer. ΔA270 × 10−6 optical units; L = 1 cm. Symbols I and II correspond to various PEG domains; arrows show the direction of the CD band amplitude change at temperature increase

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: Dependence of the amplitude of the abnormal band (λ = 270 nm) in the CD spectra of DNA LCDs formed at room temperature (curve 1), heated to 80 °C (curve 2) and cooled to room temperature (curve 3) on PEG concentration. CDNA = 30 μg ml−1, 0.3 M NaCl + 0.002 M Na+ phosphate buffer. ΔA270 × 10−6 optical units; L = 1 cm. Symbols I and II correspond to various PEG domains; arrows show the direction of the CD band amplitude change at temperature increase

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques: Concentration Assay

CD spectra of DNA LCD treated with SYBR Green (curves 2–5), measured at different temperatures. 2–22 °C, 3–40 °C, 4–80 °C, 5–80 °C → 22 °C. CDNA = 30 μg ml−1, CPEG = 250 mg ml−1, 0.3 M NaCl + 0.002 M Na+ phosphate buffer. CSG = 0.781 × 10−5 M. 1 – the CD spectrum of the initial DNA LCD (22 °C, control). ΔA = (AL – AR) × 10−6 optical units, L = 1 cm

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: CD spectra of DNA LCD treated with SYBR Green (curves 2–5), measured at different temperatures. 2–22 °C, 3–40 °C, 4–80 °C, 5–80 °C → 22 °C. CDNA = 30 μg ml−1, CPEG = 250 mg ml−1, 0.3 M NaCl + 0.002 M Na+ phosphate buffer. CSG = 0.781 × 10−5 M. 1 – the CD spectrum of the initial DNA LCD (22 °C, control). ΔA = (AL – AR) × 10−6 optical units, L = 1 cm

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques: SYBR Green Assay

Hypothetical scheme of hexagonal ds DNA molecule packing in dispersion particles formed in PEG-containing solution. (Dark spots are PEG-molecules outside closely packed DNA molecules) (See text above this Fig.)

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: Hypothetical scheme of hexagonal ds DNA molecule packing in dispersion particles formed in PEG-containing solution. (Dark spots are PEG-molecules outside closely packed DNA molecules) (See text above this Fig.)

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques:

Theoretically computed CD spectra of ds DNA cholesteric LCD particles: curve 1 – CD spectrum for classical ds DNA cholesteric LCD (d = 3.5 nm, P = 2,500 nm, φ = 0.5°); curve 2 – CD spectrum for model artificial ds DNA cholesteric LCD (d = 2.5 nm, φ = 0.5°); curve 3 - CD spectrum for model artificial ds DNA cholesteric LCD (d = 2.5 nm, φ = 1.0°); curve 4 - CD spectrum for model artificial ds DNA cholesteric LCD (d = 2.5 nm, φ = 1.5°). D = 550 nm, CDNA = 30 μg ml−1, ΔA = (AL – AR) × 10−6 optical units, L = 1 cm. φ - the twist angle between neighboring quasinematic layers

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: Theoretically computed CD spectra of ds DNA cholesteric LCD particles: curve 1 – CD spectrum for classical ds DNA cholesteric LCD (d = 3.5 nm, P = 2,500 nm, φ = 0.5°); curve 2 – CD spectrum for model artificial ds DNA cholesteric LCD (d = 2.5 nm, φ = 0.5°); curve 3 - CD spectrum for model artificial ds DNA cholesteric LCD (d = 2.5 nm, φ = 1.0°); curve 4 - CD spectrum for model artificial ds DNA cholesteric LCD (d = 2.5 nm, φ = 1.5°). D = 550 nm, CDNA = 30 μg ml−1, ΔA = (AL – AR) × 10−6 optical units, L = 1 cm. φ - the twist angle between neighboring quasinematic layers

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques:

Theoretically calculated dependence of the abnormal negative band amplitude (∆A 270) upon the pitches (P) of hypothetical ds DNA cholesterics. Point 1 (green) corresponds to the real parameters of ds DNA classical cholesteric. Point 2 (red) corresponds to the P value that is equal to about 500 nm. CDNA = 30 μg ml−1, ΔA270 × 10−6 optical units, L = 1 cm

Journal: Journal of Biological Physics

Article Title: Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

doi: 10.1007/s10867-016-9433-4

Figure Lengend Snippet: Theoretically calculated dependence of the abnormal negative band amplitude (∆A 270) upon the pitches (P) of hypothetical ds DNA cholesterics. Point 1 (green) corresponds to the real parameters of ds DNA classical cholesteric. Point 2 (red) corresponds to the P value that is equal to about 500 nm. CDNA = 30 μg ml−1, ΔA270 × 10−6 optical units, L = 1 cm

Article Snippet: Calf thymus depolymerized ds DNA (Sigma, USA) with a molecular mass of ∼(0.6–0.8) × 10 6 Da after additional purification was used.

Techniques: