Incubation:Article Title: Derepressing nuclear pyruvate dehydrogenase induces therapeutic cancer cell reprogramming.
Article Snippet: In brief Zhao et al. uncovered a nuclear-restricted acetyl-CoA regulatory mechanism where nucleus-localized pyruvate dehydrogenase complex (nPDC) is constitutively bound and repressed by nuclear protein ELMSAN1.. Pharmacological disruption of the ELMSAN1-nPDC interaction derepresses nPDC activity, synergizes with HDAC1/2 inhibitors, and therapeutically reprograms diverse cancer types to durably lose proliferation, stemness, and cell-of-origin identity.. Pyruvate
Article Title: Supporting Information for Mapping seasonal migration in a songbird hybrid zone; heritability, genetic correlations and genomic patterns linked to speciation
Article Snippet: .. Briefly, for tagmentation 2-5 nanograms of DNA was added to a mix of TDE1 Illumina Buffer, homemade buffer (20mM Tris-Hcl, 10mM MgCl2) and Tn5 transposase enzyme (TDE1 - Illumina) pre-charged with custom adapters and incubated at 55°C for 5 minutes. ..
Article Title: Supporting Information for S1 basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs
Article Snippet: .. Nuclei were resuspended in 50 μl of tagmentation reaction mix as per manufacturer instructions (TDE1, Illumina) and incubated at 37°C for 30 mins with gentle agitation every 5 min. .. Reactions were purified following the manufacturer’s instructions using a QIAGEN MiniElute PCR purification kit (https://www.qiagen.com) and eluted in 11 μl of elution buffer.
Polymerase Chain Reaction:Article Title: Derepressing nuclear pyruvate dehydrogenase induces therapeutic cancer cell reprogramming.
Article Snippet: In brief Zhao et al. uncovered a nuclear-restricted acetyl-CoA regulatory mechanism where nucleus-localized pyruvate dehydrogenase complex (nPDC) is constitutively bound and repressed by nuclear protein ELMSAN1.. Pharmacological disruption of the ELMSAN1-nPDC interaction derepresses nPDC activity, synergizes with HDAC1/2 inhibitors, and therapeutically reprograms diverse cancer types to durably lose proliferation, stemness, and cell-of-origin identity.. Pyruvate
Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
other:
Gentle:Article Title: Supporting Information for S1 basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs
Article Snippet: .. Nuclei were resuspended in 50 μl of tagmentation reaction mix as per manufacturer instructions (TDE1, Illumina) and incubated at 37°C for 30 mins with gentle agitation every 5 min. .. Reactions were purified following the manufacturer’s instructions using a QIAGEN MiniElute PCR purification kit (https://www.qiagen.com) and eluted in 11 μl of elution buffer.
cDNA Synthesis:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Purification:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Real-time Polymerase Chain Reaction:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Crocin Bleaching Assay:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Enzyme-linked Immunosorbent Assay:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Software:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Imaging:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Magnetic Beads:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
Membrane:Article Title: Malaria-derived hemozoin skews dendritic cell responses to bacterial infections by reducing interferon-stimulated-gene transcription by SWI/SNF-NuRD
Article Snippet: .. Biology grade Merck, Sigma Aldrich Cat#: 47608-250 ml Phenol, 89% solution Merck, Sigma Aldrich Cat#: P9346 Critical commercial assays Ficoll-PaqueTM PLUS Cytiva Cat#: 17-1440-03 Jo urn al Pr e-p roo f EasySepTM human monocyte kit STEMCELL Technologies Cat#: 19059 Percoll Sigma Aldrich Cat#: P1644 Tri Reagents ThermoFisher Scientific Cat#: AM9738 Superscript VILO cDNA Synthesis kit ThermoFisher Scientific Cat#: 11754050 Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) Illumina N/A MinElute PCR purification kit Qiagen Cat#: 28004 Clarity -ECL-Western substates BioRad Cat#: 1705060 Kapa SYBR FAST qPCR mix Roche/KAPA Biosystems, Sigma Aldrich Cat#: KK4618 CBA human Inflammatory Cytokine Kit BD Biosciences Cat# 551811 CBA human Chemokine kit BD Biosciences Cat# 552990 Elisa FLEX: human TNF-α (ALP) Mabtech Cat# 3512-1A-6 Elisa FLEX: human IL-6 (ALP) Mabtech Cat# 3460-1A-6 Elisa FLEX: human IL-23 (ALP) Mabtech Cat# 3457-1A-6 Elisa FLEX: human IL-10 (ALP) Mabtech Cat# 3430-1A-6 Elisa FLEX: human IL-1 (ALP) Mabtech Cat# 3416-1A-6 Human IL-1ra/IL-1F3 DuoSet ELISA Biotechne, R&D systems Cat# DY280 Experimental models: Organisms/strains Human buffy coats Stockholm Blood Centre N/A Oligonucleotides Presented in Supplementary Table S1 Software and algorithms Jo urn al Pr e-p roo f SoftMax Pro 5.2 rev C software Molecular Devices Corp ImageLab (ChemiDocTM Imaging system) BioRad FlowJo software v10.10 BD Biosciences Graph Pad Prism software v10.4.1 GraphPad software BioRender software (used for Fig. 1A, Fig. 7, Graphical abstract) BioRender https://biorender.co m; ConTra v3, update 2017 Inflammation Research Centre, Ghent University Łukasz Kreft et al.87 Other Protein A/G magnetic beads ThermoFisher Scientific Cat#: LSKMAGAG PVDF immobilon membrane Milipore/Sigma Aldrich Cat#: HVLP04700 Jo urn al Pr e-p roo f ..
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