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Dade Behring diffquick method
Diffquick Method, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quik+stained+method/diffquick+kit/ppr0250671-63-10-12
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diffquick method - by Bioz Stars, 2026-11
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Cell Culture:

Article Title: Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts
Article Snippet: .. After 7 days of cell culture and one medium change, cell-seeded scaffolds were stained with DiffQuick kit (Dade Behring Inc., Newark, DE), 4’,6-diamidino2-phenylindole, and Live=Dead (Promega, Inc.) stains. .. Burst pressures were determined using a peristaltic pump and a piezoelectric pressure transducer (Endevco, San Juan, CA) connected to a personal computer through a universal serial bus–interfaced data-acquisition module (Omega Engineering Inc., Bridgeport, NJ).

Article Title: Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts
Article Snippet: .. After 7 days of cell culture and one medium change, cell-seeded scaffolds were stained with DiffQuick kit (Dade Behring Inc., Newark, DE), 4',6-diamidino-2-phenylindole, and Live/Dead (Promega, Inc.) stains. .. Burst pressures were determined using a peristaltic pump and a piezoelectric pressure transducer (Endevco, San Juan, CA) connected to a personal computer through a universal serial bus–interfaced data-acquisition module (Omega Engineering Inc., Bridgeport, NJ).

Staining:

Article Title: Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts
Article Snippet: .. After 7 days of cell culture and one medium change, cell-seeded scaffolds were stained with DiffQuick kit (Dade Behring Inc., Newark, DE), 4’,6-diamidino2-phenylindole, and Live=Dead (Promega, Inc.) stains. .. Burst pressures were determined using a peristaltic pump and a piezoelectric pressure transducer (Endevco, San Juan, CA) connected to a personal computer through a universal serial bus–interfaced data-acquisition module (Omega Engineering Inc., Bridgeport, NJ).

Article Title: Effect of Multi-Walled Carbon Nanotubes in the Snail Cornu aspersum
Article Snippet: .. Morphometric alterations in hemocytes were determined by image analysis of DiffQuick® (Dade Behring, Newark, NJ, USA) stained cells [57,59]. ..

Article Title: TGF-β-mediated airway tolerance to allergens induced by peptide-based immunomodulatory mucosal vaccination.
Article Snippet: The pellets were used for total and differential cell counts as previously described.33 Briefly, red blood cells were lysed using ACK lysing buffer (PharM Lyse, BD Biosciences, Mississauga, ON, Canada). .. Cells were spun onto glass slides and stained using the Diffquick method (Dade Behring, Deerfield, IL). ..

Article Title: Trained Immunity Induced by in Vivo Peptide-Based STAT6 Inhibition Prevents Ragweed Allergy in Mice
Article Snippet: Brie y, red blood cells were lysed using ACK lysing buffer (PharM LyseTM, BD Biosciences, Mississauga, ON). .. Cells were spun onto glass slides and stained using the Diffquick method (Dade Behring Inc IL). ..

Article Title: Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts
Article Snippet: .. After incubation, the nonmigrated cells were removed using a wiper blade (NeuroProbe, Gaithersburg, MD) and the filter fixed and stained using a DiffQuick kit (Dade Behring Inc, Newark, DE), dried, and screened for migrated cells using an inverted microscope. ..

Article Title: Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts
Article Snippet: .. After 7 days of cell culture and one medium change, cell-seeded scaffolds were stained with DiffQuick kit (Dade Behring Inc., Newark, DE), 4',6-diamidino-2-phenylindole, and Live/Dead (Promega, Inc.) stains. .. Burst pressures were determined using a peristaltic pump and a piezoelectric pressure transducer (Endevco, San Juan, CA) connected to a personal computer through a universal serial bus–interfaced data-acquisition module (Omega Engineering Inc., Bridgeport, NJ).

other:

Article Title: Antibodies blocking adhesion and matrix binding domains of laminin-332 inhibit tumor growth and metastasis in vivo.
Article Snippet: tumor growth and metastasis in vivo Sirpa Salo, Ariel Boutaud, Anker Jon Hansen, Liqun He, Yi Sun, Sylvia Morales, Amy Venturini, Paula Martin, Pasi Nokelainen, Christer Betsholtz, Ida Stenfeldt Mathiasen and Karl Tryggvason* Department of Biochemistry, University of Oulu, Oulu, Finland BioStratum Inc., Durham, NC Biopharmaceutical Research Unit, Novo Nordisk A/S, Målov, Denmark Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden

Incubation:

Article Title: Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts
Article Snippet: .. After incubation, the nonmigrated cells were removed using a wiper blade (NeuroProbe, Gaithersburg, MD) and the filter fixed and stained using a DiffQuick kit (Dade Behring Inc, Newark, DE), dried, and screened for migrated cells using an inverted microscope. ..

Inverted Microscopy:

Article Title: Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts
Article Snippet: .. After incubation, the nonmigrated cells were removed using a wiper blade (NeuroProbe, Gaithersburg, MD) and the filter fixed and stained using a DiffQuick kit (Dade Behring Inc, Newark, DE), dried, and screened for migrated cells using an inverted microscope. ..



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Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics <t>using</t> <t>Diff-Quik</t> staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
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Image Search Results


Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Journal: Clinical and Experimental Otorhinolaryngology

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

doi: 10.21053/ceo.2025-00170

Figure Lengend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Article Snippet: Slides were stained using the Diff-Quik staining method (Sysmex).

Techniques: Diff-Quik, Staining, Standard Deviation