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Siemens Healthineers diff-quik solutions i
Diff Quik Solutions I, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Staining:

Article Title: Macrophage P2Y6 Receptor Signaling Selectively Activates NFATC2 and Suppresses Allergic Lung Inflammation.
Article Snippet: .. Bronchoalveolar lavage (BAL) fluid cells were cytocentrifuged onto slides, stained with DiffQuik (Siemens Healthcare Diagnostics), and differentially counted. .. The concentration of IL-12p40 in supernatants from the ex vivo BMMs was measured by using commercially available ELISA (BD Biosciences) kits according to the instructions of the manufacturer.

other:

Article Title: Tibial plateau levelling osteotomy: significance of matrix metalloproteinases in long-term monitoring of canine stifle stabilization after cranial cruciate ligament rupture
Article Snippet: Synovial fluid was collected via arthrocentesis and the smears were made and stained with Diff-Quik (Siemens Healthineers, Surrey, UK) for cytological analyses, and determination of total nucleated cell count was performed.

Article Title: MTOR maintains endothelial cell integrity to limit lung vascular injury
Article Snippet: Differential staining of BAL cells was performed using RAL Diff-Quik (Siemens Medical Solutions).

Article Title: Preclinical feasibility of bronchoscopic fluorescence-guided lung sentinel lymph node mapping.
Article Snippet: Methods: Healthy Yorkshire pigs were used; sentinel lymph node mapping was performed with indocyanine green.. The primary fluorescence imaging method was an ultrathin composite fiberscope placed in the bronchoscope working channel.. Secondary methods used a fluorescence thoracoscope placed in the trachea (rigid bronchoscopy) and pretracheal fascial plane (mediastinoscopy) to validate ultrathin composite fiberscope settings for sentinel lymph node detection.

Article Title: Canine Mast Cell Tumors: Clinical Signs, Laboratory Diagnosis, Treatment, and Prognosis
Article Snippet: Canine mast cell tumors, a tumor originating from mast cells involved in allergic reactions and inflammation, are among the most common skin tumors in dogs.. The present study aimed to explore the clinical features, diagnostic approaches, and prognosis of canine mastocytomas through a case study.. A 5-year-old male Akita, weighing 35.8 kg, was brought to the Doctor VET veterinary clinic in Kamianets-Podilskyi, Ukraine, for evaluation.

Article Title: Chronic Coinfection with Pseudomonas aeruginosa and Normal Colony Staphylococcus aureus Causes Lung Structural Damage in the Cystic Fibrosis Rat.
Article Snippet: 82 83 84 Gretchen E. Bollar,*y Johnathan D. Keith,y Denise D. Stanford,*y Ashley M. Oden,* S. Vamsee Raju,*y T. Spencer Poore,yz and Susan E. Birket*y 85 86 87 From the Division of Pulmonary, Allergy, and Critical Care Medicine,* Department of Medicine, the Gregory Fleming James Cystic Fibrosis Research Center,y and the Division of Pulmonology and Sleep Medicine,z Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama 88 89 90 Accepted for publication

Article Title: Therapeutic efficacy of JEW-M449, an anti-TCTP monoclonal antibody, administered via the nasal route in a BALB/c mouse model of ovalbumin-induced acute asthma.
Article Snippet: Numerous studies have highlighted the role of translationally controlled tumor protein (TCTP) as a key inflammatory mediator of asthma and allergies.. Our previous study revealed that blocking the cytokine-like activity of TCTP using JEW-M449, an anti-TCTP monoclonal antibody (mAb), alleviated allergic inflammation in asthmatic mice.. This study aimed to determine whether directly delivering JEW-M449 into the respiratory tract is a more effective way of mitigating airway inflammation in a mouse model of ovalbumin (OVA)-induced allergic airway inflammation than delivering this antibody via the intraperitoneal (IP) route.

Article Title: MTOR maintains endothelial cell integrity to limit lung vascular injury.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics <t>using</t> <t>Diff-Quik</t> staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
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Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics <t>using</t> <t>Diff-Quik</t> staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
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Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics <t>using</t> <t>Diff-Quik</t> staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
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Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Journal: Clinical and Experimental Otorhinolaryngology

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

doi: 10.21053/ceo.2025-00170

Figure Lengend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Article Snippet: Slides were stained using the Diff-Quik staining method (Sysmex).

Techniques: Diff-Quik, Staining, Standard Deviation