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synthetic fluorogenic substrates ac-devd-afc  (Bachem)

 
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    Bachem synthetic fluorogenic substrates ac-devd-afc
    Synthetic Fluorogenic Substrates Ac Devd Afc, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/devd+afc/fluorogenic+substrate+ac+devd+afc/pmc01539015-57-12-16
    Average 90 stars, based on 1 article reviews
    synthetic fluorogenic substrates ac-devd-afc - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
    Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

    Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
    Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

    Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
    Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

    Article Title: Characterization of caspase-2 inhibitors based on specific sites of caspase-2-mediated proteolysis.
    Article Snippet: AFC fluorogenic substrates Z‐VDVAD‐AFC and AcDEVD‐AFC and control peptides AcVDVAD‐CHO and AcDEVD‐CHO were purchased from Bachem (Torrance).

    Article Title: Characterization of caspase-2 inhibitors based on specific sites of caspase-2-mediated proteolysis
    Article Snippet: AFC fluorogenic substrates Z-VDVAD-AFC and AcDEVD-AFC and control peptides AcVDVAD-CHO and AcDEVD-CHO were purchased from Bachem (Torrance/CA, USA).

    Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
    Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

    Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
    Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

    Control:

    Article Title: Characterization of caspase-2 inhibitors based on specific sites of caspase-2-mediated proteolysis
    Article Snippet: Human recombinant Casp1, 6, 7, and 9 were purchased from BioVision (Milpitas/CA, USA). .. AFC fluorogenic substrates and control peptides (AcYVAD-CHO, AcVDVAD-CHO, AcDEVD-CHO, AcVEID-CHO, and AcLEHD-CHO) were purchased from Bachem (Torrance/CA, USA). ..



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    Journal: Neoplasia (New York, N.Y.)

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    Figure Lengend Snippet: Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

    Article Snippet: The payloads used for antibodies conjugation, MC-GGFG-DXD (DXd, catalog reference: HY-13631E), MC-Val-Cit-PAB-MMAF (Vc-MMAF, catalog reference: HY-112786) and McMMAF (catalog reference: HY-15578), the belantamab mafodotin ADC (catalog reference: HY-P3239), as well as the Ac-DEVD-AFC (catalog reference: HY-P1005) substrate for caspase-3 activity detection, were purchased from MedChem Express (Princeton, NJ, USA).

    Techniques: Flow Cytometry, Apoptosis Assay, Western Blot, Control, Molecular Weight, Activity Assay, Fluorescence