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Enzo Biochem devd-afc
Devd Afc, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/devd+afc/ac+devd+afc/pmc07410077-76-0-1
Average 90 stars, based on 1 article reviews
devd-afc - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Incubation:

Article Title: A Quantitative Method for the Specific Assessment of Caspase-6 Activity in Cell Culture
Article Snippet: Ac-VEID-Afc, Ac-DEVD-Afc, Ac-VEID-CHO, zVAD-fmk and active caspase-3, -6 and -7 enzymes were purchased from Enzo Biosciences.


Article Title: Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells.
Article Snippet: The cell lysates were diluted and transferred to black 384-well plates, followed by the addition of an equal volume of Ac-DEVD-AFC (Enzo, Farmingdale, NY, USA)-containing reaction buffer.

Article Title: Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors.
Article Snippet: High throughput cell viability screening assays often capitalize on the ability of active enzymes or molecules within viable cells to catalyze a quantifiable chemical reaction.. The tetrazolium reduction (MTT) assay relies on oxidoreductases to reduce tetrazolium into purple formazan crystals that are solubilized so absorbance reflects viability, while other assays use cellular ATP to catalyze a luminescence-emitting reaction.. It is therefore important to know how accurately these assays report cellular responses, as cytotoxic anti-cancer agents promote cell death via a variety of signaling pathways, some of which may alter how these assays work.

Article Title: Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells
Article Snippet: Cell lysates were incubated with reaction buffer containing Ac-DEVD-AFC (Enzo, USA).

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout
Article Snippet: To induce apoptosis, THP-1 cells were treated with Staurosporine (1 μg mL −1 , 60 min, Sigma Aldrich), lysed as above and incubated with the fluorogenic caspase-3 substrate Ac-DEVD-AFC (20 μM, Enzo Life Sciences) or the caspase-9 fluorogenic substrate Ac-LEHD-AFC (100 μM, Enzo Life Sciences).

Article Title: Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)–Mixed Lineage Kinase Domain-Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers
Article Snippet: 36 Briefly, 15 μg of total proteins were combined with 2 μmol/L Ac-IETD-AFC or Ac-DEVD-AFC (Enzo Life Sciences) in a caspase assay buffer [100 mmol/L NaCI, 1 mmol/L EDTA, 20 mmol/L PIPES (piperazine-N,N′-bis), 10% (w/v) sucrose, 0.1% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), 10 mmol/L dithiothreitol, pH 7.2] in a final volume of 200 μL and were incubated for 2 hours at 37°C.

Article Title: Molecular insights on cytochrome c and nucleotide regulation of apoptosome function and its implication in cancer
Article Snippet: The fluorogenic substrates LEHD-AFC and DEVD-AFC were obtained from Enzo Life Sciences.

Fluorescence:

Article Title: A Quantitative Method for the Specific Assessment of Caspase-6 Activity in Cell Culture
Article Snippet: Ac-VEID-Afc, Ac-DEVD-Afc, Ac-VEID-CHO, zVAD-fmk and active caspase-3, -6 and -7 enzymes were purchased from Enzo Biosciences.


Article Title: Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells.
Article Snippet: The cell lysates were diluted and transferred to black 384-well plates, followed by the addition of an equal volume of Ac-DEVD-AFC (Enzo, Farmingdale, NY, USA)-containing reaction buffer.

Article Title: Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors.
Article Snippet: High throughput cell viability screening assays often capitalize on the ability of active enzymes or molecules within viable cells to catalyze a quantifiable chemical reaction.. The tetrazolium reduction (MTT) assay relies on oxidoreductases to reduce tetrazolium into purple formazan crystals that are solubilized so absorbance reflects viability, while other assays use cellular ATP to catalyze a luminescence-emitting reaction.. It is therefore important to know how accurately these assays report cellular responses, as cytotoxic anti-cancer agents promote cell death via a variety of signaling pathways, some of which may alter how these assays work.

Article Title: Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells
Article Snippet: Cell lysates were incubated with reaction buffer containing Ac-DEVD-AFC (Enzo, USA).

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout
Article Snippet: To induce apoptosis, THP-1 cells were treated with Staurosporine (1 μg mL −1 , 60 min, Sigma Aldrich), lysed as above and incubated with the fluorogenic caspase-3 substrate Ac-DEVD-AFC (20 μM, Enzo Life Sciences) or the caspase-9 fluorogenic substrate Ac-LEHD-AFC (100 μM, Enzo Life Sciences).

Article Title: Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)–Mixed Lineage Kinase Domain-Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers
Article Snippet: 36 Briefly, 15 μg of total proteins were combined with 2 μmol/L Ac-IETD-AFC or Ac-DEVD-AFC (Enzo Life Sciences) in a caspase assay buffer [100 mmol/L NaCI, 1 mmol/L EDTA, 20 mmol/L PIPES (piperazine-N,N′-bis), 10% (w/v) sucrose, 0.1% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), 10 mmol/L dithiothreitol, pH 7.2] in a final volume of 200 μL and were incubated for 2 hours at 37°C.

Article Title: Molecular insights on cytochrome c and nucleotide regulation of apoptosome function and its implication in cancer
Article Snippet: The fluorogenic substrates LEHD-AFC and DEVD-AFC were obtained from Enzo Life Sciences.

Generated:

Article Title: A Quantitative Method for the Specific Assessment of Caspase-6 Activity in Cell Culture
Article Snippet: Ac-VEID-Afc, Ac-DEVD-Afc, Ac-VEID-CHO, zVAD-fmk and active caspase-3, -6 and -7 enzymes were purchased from Enzo Biosciences.


Article Title: Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells.
Article Snippet: The cell lysates were diluted and transferred to black 384-well plates, followed by the addition of an equal volume of Ac-DEVD-AFC (Enzo, Farmingdale, NY, USA)-containing reaction buffer.

Article Title: Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors.
Article Snippet: High throughput cell viability screening assays often capitalize on the ability of active enzymes or molecules within viable cells to catalyze a quantifiable chemical reaction.. The tetrazolium reduction (MTT) assay relies on oxidoreductases to reduce tetrazolium into purple formazan crystals that are solubilized so absorbance reflects viability, while other assays use cellular ATP to catalyze a luminescence-emitting reaction.. It is therefore important to know how accurately these assays report cellular responses, as cytotoxic anti-cancer agents promote cell death via a variety of signaling pathways, some of which may alter how these assays work.

Article Title: Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells
Article Snippet: Cell lysates were incubated with reaction buffer containing Ac-DEVD-AFC (Enzo, USA).

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout
Article Snippet: To induce apoptosis, THP-1 cells were treated with Staurosporine (1 μg mL −1 , 60 min, Sigma Aldrich), lysed as above and incubated with the fluorogenic caspase-3 substrate Ac-DEVD-AFC (20 μM, Enzo Life Sciences) or the caspase-9 fluorogenic substrate Ac-LEHD-AFC (100 μM, Enzo Life Sciences).

Article Title: Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)–Mixed Lineage Kinase Domain-Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers
Article Snippet: 36 Briefly, 15 μg of total proteins were combined with 2 μmol/L Ac-IETD-AFC or Ac-DEVD-AFC (Enzo Life Sciences) in a caspase assay buffer [100 mmol/L NaCI, 1 mmol/L EDTA, 20 mmol/L PIPES (piperazine-N,N′-bis), 10% (w/v) sucrose, 0.1% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), 10 mmol/L dithiothreitol, pH 7.2] in a final volume of 200 μL and were incubated for 2 hours at 37°C.

Article Title: Molecular insights on cytochrome c and nucleotide regulation of apoptosome function and its implication in cancer
Article Snippet: The fluorogenic substrates LEHD-AFC and DEVD-AFC were obtained from Enzo Life Sciences.

Concentration Assay:

Article Title: A Quantitative Method for the Specific Assessment of Caspase-6 Activity in Cell Culture
Article Snippet: Ac-VEID-Afc, Ac-DEVD-Afc, Ac-VEID-CHO, zVAD-fmk and active caspase-3, -6 and -7 enzymes were purchased from Enzo Biosciences.


Article Title: Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells.
Article Snippet: The cell lysates were diluted and transferred to black 384-well plates, followed by the addition of an equal volume of Ac-DEVD-AFC (Enzo, Farmingdale, NY, USA)-containing reaction buffer.

Article Title: Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors.
Article Snippet: High throughput cell viability screening assays often capitalize on the ability of active enzymes or molecules within viable cells to catalyze a quantifiable chemical reaction.. The tetrazolium reduction (MTT) assay relies on oxidoreductases to reduce tetrazolium into purple formazan crystals that are solubilized so absorbance reflects viability, while other assays use cellular ATP to catalyze a luminescence-emitting reaction.. It is therefore important to know how accurately these assays report cellular responses, as cytotoxic anti-cancer agents promote cell death via a variety of signaling pathways, some of which may alter how these assays work.

Article Title: Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells
Article Snippet: Cell lysates were incubated with reaction buffer containing Ac-DEVD-AFC (Enzo, USA).

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout
Article Snippet: To induce apoptosis, THP-1 cells were treated with Staurosporine (1 μg mL −1 , 60 min, Sigma Aldrich), lysed as above and incubated with the fluorogenic caspase-3 substrate Ac-DEVD-AFC (20 μM, Enzo Life Sciences) or the caspase-9 fluorogenic substrate Ac-LEHD-AFC (100 μM, Enzo Life Sciences).

Article Title: Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)–Mixed Lineage Kinase Domain-Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers
Article Snippet: 36 Briefly, 15 μg of total proteins were combined with 2 μmol/L Ac-IETD-AFC or Ac-DEVD-AFC (Enzo Life Sciences) in a caspase assay buffer [100 mmol/L NaCI, 1 mmol/L EDTA, 20 mmol/L PIPES (piperazine-N,N′-bis), 10% (w/v) sucrose, 0.1% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), 10 mmol/L dithiothreitol, pH 7.2] in a final volume of 200 μL and were incubated for 2 hours at 37°C.

Article Title: Molecular insights on cytochrome c and nucleotide regulation of apoptosome function and its implication in cancer
Article Snippet: The fluorogenic substrates LEHD-AFC and DEVD-AFC were obtained from Enzo Life Sciences.

SDS Page:

Article Title: A Quantitative Method for the Specific Assessment of Caspase-6 Activity in Cell Culture
Article Snippet: Ac-VEID-Afc, Ac-DEVD-Afc, Ac-VEID-CHO, zVAD-fmk and active caspase-3, -6 and -7 enzymes were purchased from Enzo Biosciences.


Article Title: Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells.
Article Snippet: The cell lysates were diluted and transferred to black 384-well plates, followed by the addition of an equal volume of Ac-DEVD-AFC (Enzo, Farmingdale, NY, USA)-containing reaction buffer.

Article Title: Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors.
Article Snippet: High throughput cell viability screening assays often capitalize on the ability of active enzymes or molecules within viable cells to catalyze a quantifiable chemical reaction.. The tetrazolium reduction (MTT) assay relies on oxidoreductases to reduce tetrazolium into purple formazan crystals that are solubilized so absorbance reflects viability, while other assays use cellular ATP to catalyze a luminescence-emitting reaction.. It is therefore important to know how accurately these assays report cellular responses, as cytotoxic anti-cancer agents promote cell death via a variety of signaling pathways, some of which may alter how these assays work.

Article Title: Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells
Article Snippet: Cell lysates were incubated with reaction buffer containing Ac-DEVD-AFC (Enzo, USA).

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout
Article Snippet: To induce apoptosis, THP-1 cells were treated with Staurosporine (1 μg mL −1 , 60 min, Sigma Aldrich), lysed as above and incubated with the fluorogenic caspase-3 substrate Ac-DEVD-AFC (20 μM, Enzo Life Sciences) or the caspase-9 fluorogenic substrate Ac-LEHD-AFC (100 μM, Enzo Life Sciences).

Article Title: Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)–Mixed Lineage Kinase Domain-Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers
Article Snippet: 36 Briefly, 15 μg of total proteins were combined with 2 μmol/L Ac-IETD-AFC or Ac-DEVD-AFC (Enzo Life Sciences) in a caspase assay buffer [100 mmol/L NaCI, 1 mmol/L EDTA, 20 mmol/L PIPES (piperazine-N,N′-bis), 10% (w/v) sucrose, 0.1% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), 10 mmol/L dithiothreitol, pH 7.2] in a final volume of 200 μL and were incubated for 2 hours at 37°C.

Article Title: Molecular insights on cytochrome c and nucleotide regulation of apoptosome function and its implication in cancer
Article Snippet: The fluorogenic substrates LEHD-AFC and DEVD-AFC were obtained from Enzo Life Sciences.

Western Blot:

Article Title: A Quantitative Method for the Specific Assessment of Caspase-6 Activity in Cell Culture
Article Snippet: Ac-VEID-Afc, Ac-DEVD-Afc, Ac-VEID-CHO, zVAD-fmk and active caspase-3, -6 and -7 enzymes were purchased from Enzo Biosciences.


Article Title: Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells.
Article Snippet: The cell lysates were diluted and transferred to black 384-well plates, followed by the addition of an equal volume of Ac-DEVD-AFC (Enzo, Farmingdale, NY, USA)-containing reaction buffer.

Article Title: Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors.
Article Snippet: High throughput cell viability screening assays often capitalize on the ability of active enzymes or molecules within viable cells to catalyze a quantifiable chemical reaction.. The tetrazolium reduction (MTT) assay relies on oxidoreductases to reduce tetrazolium into purple formazan crystals that are solubilized so absorbance reflects viability, while other assays use cellular ATP to catalyze a luminescence-emitting reaction.. It is therefore important to know how accurately these assays report cellular responses, as cytotoxic anti-cancer agents promote cell death via a variety of signaling pathways, some of which may alter how these assays work.

Article Title: Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells
Article Snippet: Cell lysates were incubated with reaction buffer containing Ac-DEVD-AFC (Enzo, USA).

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout
Article Snippet: To induce apoptosis, THP-1 cells were treated with Staurosporine (1 μg mL −1 , 60 min, Sigma Aldrich), lysed as above and incubated with the fluorogenic caspase-3 substrate Ac-DEVD-AFC (20 μM, Enzo Life Sciences) or the caspase-9 fluorogenic substrate Ac-LEHD-AFC (100 μM, Enzo Life Sciences).

Article Title: Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)–Mixed Lineage Kinase Domain-Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers
Article Snippet: 36 Briefly, 15 μg of total proteins were combined with 2 μmol/L Ac-IETD-AFC or Ac-DEVD-AFC (Enzo Life Sciences) in a caspase assay buffer [100 mmol/L NaCI, 1 mmol/L EDTA, 20 mmol/L PIPES (piperazine-N,N′-bis), 10% (w/v) sucrose, 0.1% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), 10 mmol/L dithiothreitol, pH 7.2] in a final volume of 200 μL and were incubated for 2 hours at 37°C.

Article Title: Molecular insights on cytochrome c and nucleotide regulation of apoptosome function and its implication in cancer
Article Snippet: The fluorogenic substrates LEHD-AFC and DEVD-AFC were obtained from Enzo Life Sciences.

Caspase Assay:

Article Title: A Quantitative Method for the Specific Assessment of Caspase-6 Activity in Cell Culture
Article Snippet: Ac-VEID-Afc, Ac-DEVD-Afc, Ac-VEID-CHO, zVAD-fmk and active caspase-3, -6 and -7 enzymes were purchased from Enzo Biosciences.


Article Title: Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells.
Article Snippet: The cell lysates were diluted and transferred to black 384-well plates, followed by the addition of an equal volume of Ac-DEVD-AFC (Enzo, Farmingdale, NY, USA)-containing reaction buffer.

Article Title: Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors.
Article Snippet: High throughput cell viability screening assays often capitalize on the ability of active enzymes or molecules within viable cells to catalyze a quantifiable chemical reaction.. The tetrazolium reduction (MTT) assay relies on oxidoreductases to reduce tetrazolium into purple formazan crystals that are solubilized so absorbance reflects viability, while other assays use cellular ATP to catalyze a luminescence-emitting reaction.. It is therefore important to know how accurately these assays report cellular responses, as cytotoxic anti-cancer agents promote cell death via a variety of signaling pathways, some of which may alter how these assays work.

Article Title: Isolation of cabbage exosome-like nanovesicles and investigation of their biological activities in human cells
Article Snippet: Cell lysates were incubated with reaction buffer containing Ac-DEVD-AFC (Enzo, USA).

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout
Article Snippet: To induce apoptosis, THP-1 cells were treated with Staurosporine (1 μg mL −1 , 60 min, Sigma Aldrich), lysed as above and incubated with the fluorogenic caspase-3 substrate Ac-DEVD-AFC (20 μM, Enzo Life Sciences) or the caspase-9 fluorogenic substrate Ac-LEHD-AFC (100 μM, Enzo Life Sciences).

Article Title: Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)–Mixed Lineage Kinase Domain-Like Protein (MLKL)–Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers
Article Snippet: 36 Briefly, 15 μg of total proteins were combined with 2 μmol/L Ac-IETD-AFC or Ac-DEVD-AFC (Enzo Life Sciences) in a caspase assay buffer [100 mmol/L NaCI, 1 mmol/L EDTA, 20 mmol/L PIPES (piperazine-N,N′-bis), 10% (w/v) sucrose, 0.1% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), 10 mmol/L dithiothreitol, pH 7.2] in a final volume of 200 μL and were incubated for 2 hours at 37°C.

Article Title: Molecular insights on cytochrome c and nucleotide regulation of apoptosome function and its implication in cancer
Article Snippet: The fluorogenic substrates LEHD-AFC and DEVD-AFC were obtained from Enzo Life Sciences.



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Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Journal: Neoplasia (New York, N.Y.)

Article Title: Rational payload selection enables high antitumoral efficacy of an anti-EGFR antibody-drug conjugate against ovarian tumors

doi: 10.1016/j.neo.2026.101295

Figure Lengend Snippet: Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Article Snippet: The payloads used for antibodies conjugation, MC-GGFG-DXD (DXd, catalog reference: HY-13631E), MC-Val-Cit-PAB-MMAF (Vc-MMAF, catalog reference: HY-112786) and McMMAF (catalog reference: HY-15578), the belantamab mafodotin ADC (catalog reference: HY-P3239), as well as the Ac-DEVD-AFC (catalog reference: HY-P1005) substrate for caspase-3 activity detection, were purchased from MedChem Express (Princeton, NJ, USA).

Techniques: Flow Cytometry, Apoptosis Assay, Western Blot, Control, Molecular Weight, Activity Assay, Fluorescence