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Serumwerk Bernburg AG optiprep density gradient
Optiprep Density Gradient, supplied by Serumwerk Bernburg AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+layers+isolate/optiprep+density+gradient/pm37932279-251-29-32
Average 90 stars, based on 1 article reviews
optiprep density gradient - by Bioz Stars, 2026-10
90/100 stars

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Isolation:

Article Title: Neutralizing monoclonal antibodies protect against human adenovirus type 55 infection in transgenic mice and tree shrews.
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by OptiPrep density gradient separation (Serumwerk Bernburg) and subjected to memory B cell sorting. .. Purified HAdV55ΔE1ΔE3 virions were labelled with Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Thermo Fisher Scientific) according to manufacturer’s guidelines.

Article Title: An AIM2 inflammasome biomimetic mineralization inhibitor for vascular dementia therapy.
Article Snippet: .. Neuron fractions were isolated by OptiPrep density gradient (Serumwerk Bernburg AG, Bernberg, Germany) following the manufacturer’s instructions. .. Purified neurons were resuspended and seeded on poly-L-lysine-coated coverslips (0.1 mg/mL) (Sigma, Theranostics 2025, Vol.

Article Title: An AIM2 inflammasome biomimetic mineralization inhibitor for vascular dementia therapy
Article Snippet: Collected hippocampus tissue was digested with 2 mg/mL papain (Macklin, Shanghai, China) and 0.1 mg/mL DNase (Macklin, Shanghai, China) at 37°C for 30 min. .. Neuron fractions were isolated by OptiPrep density gradient (Serumwerk Bernburg AG, Bernberg, Germany) following the manufacturer ' s instructions. .. Neuron fractions were isolated by OptiPrep density gradient (Serumwerk Bernburg AG, Bernberg, Germany) following the manufacturer ' s instructions.

FACS:

Article Title: Neutralizing monoclonal antibodies protect against human adenovirus type 55 infection in transgenic mice and tree shrews.
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by OptiPrep density gradient separation (Serumwerk Bernburg) and subjected to memory B cell sorting. .. Purified HAdV55ΔE1ΔE3 virions were labelled with Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Thermo Fisher Scientific) according to manufacturer’s guidelines.

other:

Article Title: Cancer EV stimulate endothelial glycolysis to fuel protein synthesis via mTOR and AMPKα activation.
Article Snippet: Cells were harvested in buffer containing 0.25 M sucrose, 1 mM EDTA, 10 mM acetic acid, protease inhibitors (Roche, 11873580001), pH 7.4, and incubated on ice for 20 min.

Single Cell:

Article Title: ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis.
Article Snippet: Cell Death and Disease (2023) 14:721 ml papain (Macklin, Shanghai, China) and 0.1mg/ml DNase (Macklin, Shanghai, China) (37° for 20minutes). .. After terminating the digestion, 50 μg/mL DNase was added, and the hippocampal tissue was triturated gently to obtain a single-cell suspension, which was subsequently layered on top of the OptiPrep density gradient (Serumwerk Bernburg AG, Bernberg, Germany). ..

Suspension:

Article Title: ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis.
Article Snippet: Cell Death and Disease (2023) 14:721 ml papain (Macklin, Shanghai, China) and 0.1mg/ml DNase (Macklin, Shanghai, China) (37° for 20minutes). .. After terminating the digestion, 50 μg/mL DNase was added, and the hippocampal tissue was triturated gently to obtain a single-cell suspension, which was subsequently layered on top of the OptiPrep density gradient (Serumwerk Bernburg AG, Bernberg, Germany). ..



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