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Solarbio Inc dapi solution
Dapi Solution, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution+c0065/dapi/ppr0438422-62-23-25
Average 90 stars, based on 1 article reviews
dapi solution - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: Bombinin-BO1 induces hepatocellular carcinoma cell-cycle arrest and apoptosis via the HSP90A-Cdc37-CDK1 axis
Article Snippet: .. Subsequently, cells were mixed with 4% paraformaldehyde for 30 min and stained with DAPI (Solarbio, C0065, 300 nM). ..

Article Title: Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm
Article Snippet: Briefly, VSMCs with different treatments were seeded onto 12-well plates and cultured in SMCM medium for 48 h. Then switched into fresh SMCM medium supplemented with EdU (10 μmol/L) and incubated for 2 h. Cells were fixed with 4% paraformaldehyde for 15 min and permeated with 0.3% Triton X-100 (T8200, Solarbio) for another 15 min. Click reaction solution configured according to the instructions was added to each well. .. After 1 h of incubation in the dark, nuclei were stained with DAPI (C0060, Solarbio) for 10 min. .. Images were acquired with Invitrogen Evos FL Auto2 microscope (Invitrogen, USA).

Article Title: Dynamic interaction of REEP5–MFN1/2 enables mitochondrial hitchhiking on tubular ER
Article Snippet: Cells were seeded on glass coverslips (WHB-12-CS; WHB), fixed with 4% formaldehyde (P6148; Sigma-Aldrich) for 10 min at room temperature, and incubated with blocking buffer (PBS + 0.4% Triton X-100 + 1% BSA + 0.02% NaN3) for 30 min at room temperature. .. Fixed cells were then stained with primary antibody at room temperature for 2 h, washed in PBS, and stained with fluorescently labeled secondary antibody for 1 h, washed in PBS, stained with DAPI (C0060; Solarbio), and mounted with Mowiol. .. The slides were analyzed under the fluorescence microscope (Zeiss).

Article Title: Mechanism of Panax notoginseng saponins modulation of miR-214-3p/NR1I3 affecting the pharmacodynamics and pharmacokinetics of warfarin
Article Snippet: .. After washing, add fluorescent secondary antibody (1ug/ml, Thermofisher) and incubate for 2 h, then stained with DAPI (1:1000, Solarbio, C0060 CHINA). ..

Article Title: FTO-mediated m 6 A demethylation of ULK1 mRNA promotes autophagy and activation of hepatic stellate cells in liver fibrosis.
Article Snippet: .. Immunofluorescence microscopy The cells were fixed with 4% paraformaldehyde for 15‒20 min, permeabilized with 0.1% Triton X-100 for 15 min, and then blocked with 1% BSA at room temperature for 30 min. Next, the cells were incubated with primary antibodies at 4°C overnight, followed by incubation with FITC-labeled secondary antibodies (Beyotime) at room temperature for 1 h. DAPI (C0065; Solarbio) was used to stain the nuclei. .. An inverted fluorescence microscope (Olympus, Tokyo, Japan) was then used to observe the fluorescence.

Incubation:

Article Title: Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm
Article Snippet: Briefly, VSMCs with different treatments were seeded onto 12-well plates and cultured in SMCM medium for 48 h. Then switched into fresh SMCM medium supplemented with EdU (10 μmol/L) and incubated for 2 h. Cells were fixed with 4% paraformaldehyde for 15 min and permeated with 0.3% Triton X-100 (T8200, Solarbio) for another 15 min. Click reaction solution configured according to the instructions was added to each well. .. After 1 h of incubation in the dark, nuclei were stained with DAPI (C0060, Solarbio) for 10 min. .. Images were acquired with Invitrogen Evos FL Auto2 microscope (Invitrogen, USA).

Article Title: FTO-mediated m 6 A demethylation of ULK1 mRNA promotes autophagy and activation of hepatic stellate cells in liver fibrosis.
Article Snippet: .. Immunofluorescence microscopy The cells were fixed with 4% paraformaldehyde for 15‒20 min, permeabilized with 0.1% Triton X-100 for 15 min, and then blocked with 1% BSA at room temperature for 30 min. Next, the cells were incubated with primary antibodies at 4°C overnight, followed by incubation with FITC-labeled secondary antibodies (Beyotime) at room temperature for 1 h. DAPI (C0065; Solarbio) was used to stain the nuclei. .. An inverted fluorescence microscope (Olympus, Tokyo, Japan) was then used to observe the fluorescence.

Immunofluorescence:

Article Title: BCI Improves Alcohol-Induced Cognitive and Emotional Impairments by Restoring pERK-BDNF.
Article Snippet: Binge drinking causes a range of problems especially damage to the nervous system, and the specific neural mechanism of brain loss and behavioral abnormalities caused by which is still unclear.. Extracellular regulated protein kinases (ERK) maintain neuronal survival, growth, and regulation of synaptic plasticity by phosphorylating specific transcription factors to regulate expression of brain-derived neurotrophic factor (BDNF).. Dual-specific phosphatase 1 (DUSP1) and DUSP6 dephosphorylate tyrosine and serine/threonine residues in ERK1/2 to inactivate them.

Article Title: FTO-mediated m 6 A demethylation of ULK1 mRNA promotes autophagy and activation of hepatic stellate cells in liver fibrosis.
Article Snippet: .. Immunofluorescence microscopy The cells were fixed with 4% paraformaldehyde for 15‒20 min, permeabilized with 0.1% Triton X-100 for 15 min, and then blocked with 1% BSA at room temperature for 30 min. Next, the cells were incubated with primary antibodies at 4°C overnight, followed by incubation with FITC-labeled secondary antibodies (Beyotime) at room temperature for 1 h. DAPI (C0065; Solarbio) was used to stain the nuclei. .. An inverted fluorescence microscope (Olympus, Tokyo, Japan) was then used to observe the fluorescence.

Labeling:

Article Title: Dynamic interaction of REEP5–MFN1/2 enables mitochondrial hitchhiking on tubular ER
Article Snippet: Cells were seeded on glass coverslips (WHB-12-CS; WHB), fixed with 4% formaldehyde (P6148; Sigma-Aldrich) for 10 min at room temperature, and incubated with blocking buffer (PBS + 0.4% Triton X-100 + 1% BSA + 0.02% NaN3) for 30 min at room temperature. .. Fixed cells were then stained with primary antibody at room temperature for 2 h, washed in PBS, and stained with fluorescently labeled secondary antibody for 1 h, washed in PBS, stained with DAPI (C0060; Solarbio), and mounted with Mowiol. .. The slides were analyzed under the fluorescence microscope (Zeiss).

Fluorescence:

Article Title: The Role of LC3-Associated Phagocytosis Inhibits the Inflammatory Response in A spergillus fumigatus Keratitis
Article Snippet: The slices were stained with FITC-conjugated goat anti-rabbit antibody (Abcam, 1:200) for 1 hour. .. Nuclei were counterstained with DAPI (Solarbio), and slides were imaged under a fluorescence microscope at 400 × magnification. ..

Microscopy:

Article Title: The Role of LC3-Associated Phagocytosis Inhibits the Inflammatory Response in A spergillus fumigatus Keratitis
Article Snippet: The slices were stained with FITC-conjugated goat anti-rabbit antibody (Abcam, 1:200) for 1 hour. .. Nuclei were counterstained with DAPI (Solarbio), and slides were imaged under a fluorescence microscope at 400 × magnification. ..

Article Title: FTO-mediated m 6 A demethylation of ULK1 mRNA promotes autophagy and activation of hepatic stellate cells in liver fibrosis.
Article Snippet: .. Immunofluorescence microscopy The cells were fixed with 4% paraformaldehyde for 15‒20 min, permeabilized with 0.1% Triton X-100 for 15 min, and then blocked with 1% BSA at room temperature for 30 min. Next, the cells were incubated with primary antibodies at 4°C overnight, followed by incubation with FITC-labeled secondary antibodies (Beyotime) at room temperature for 1 h. DAPI (C0065; Solarbio) was used to stain the nuclei. .. An inverted fluorescence microscope (Olympus, Tokyo, Japan) was then used to observe the fluorescence.



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