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Keygen Biotech dab substrate solution
Dab Substrate Solution, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dab+substrate+solution/dab+peroxidase+substrate/pm42145053-112-5-17
Average 86 stars, based on 1 article reviews
dab substrate solution - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Staining:

Article Title: Cellular fibronectin exacerbates α-synuclein aggregation via integrin alpha4beta1 mediated PARP1 and SCD elevation
Article Snippet: .. The signals were detected with a DAB staining kit (KeyGEN, China). .. For the quantification of TH + cell numbers in each group, TH + cells in SNpc were conducted at regular, predetermined intervals using a counting frame size of 80 μm × 80 μm and a sampling grid spacing of 100 μm × 100 μm in a Leica DM6 photomicroscope equipped with a digital camera and StereoInvestigator software [ ].

Article Title: Fibrinogen exacerbates α-synuclein aggregation and mitochondrial dysfunction via alpha5beta3 integrin in Parkinson’s disease
Article Snippet: .. The signals were detected with a DAB staining kit (KeyGEN Biotech, Cat# KGB4101). ..

Article Title: Cellular fibronectin exacerbates α-synuclein aggregation via integrin alpha4beta1 mediated PARP1 and SCD elevation.
Article Snippet: The sections were then incubated with donkey anti-rabbit IgG (Alexa Fluor 488, Abcam, Catalogue number ab150073, 1:200 dilution) and/or donkey anti-mouse IgG (Alexa Fluor 594, Abcam, Catalogue number ab150108, 1:200 dilution) secondary antibodies, and the nuclei were counterstained with DAPI solution. .. For immunohistochemistry (IHC), the thickness of the brain paraffin section was 40 μm, four sections through the substantia nigra within the range covering the entire SNpc (between − 2.8 and − 3.88 mm AP from bregma) were processed for TH immunohistochemistry. the brain slices were incubated with 3 % H2O2, blocked with 10 % goat serum, and incubated with primary antibodies overnight at 4 ◦ C. The signals were detected with a DAB staining kit (KeyGEN, China). .. For the quantification of TH + cell numbers in each group, TH + cells in SNpc were conducted at regular, predetermined intervals using a counting frame size of 80 μm × 80 μm and a sampling grid spacing of 100 μm × 100 μm in a Leica DM6 photomicroscope equipped with a digital camera and StereoInvestigator software [30].

Incubation:

Article Title: Loss of DJ-1 alleviates microglia-mediated neuroinflammation in Parkinson’s disease via autophagy-lysosomal degradation of NLRP3
Article Snippet: .. After PBS washes, sections were incubated with secondary antibodyfor 1.5 h at room temperature, followed by streptavidin-HRP and development with DAB substrate (KGP1045, KeyGEN BioTECH). ..

Article Title: Cellular fibronectin exacerbates α-synuclein aggregation via integrin alpha4beta1 mediated PARP1 and SCD elevation.
Article Snippet: The sections were then incubated with donkey anti-rabbit IgG (Alexa Fluor 488, Abcam, Catalogue number ab150073, 1:200 dilution) and/or donkey anti-mouse IgG (Alexa Fluor 594, Abcam, Catalogue number ab150108, 1:200 dilution) secondary antibodies, and the nuclei were counterstained with DAPI solution. .. For immunohistochemistry (IHC), the thickness of the brain paraffin section was 40 μm, four sections through the substantia nigra within the range covering the entire SNpc (between − 2.8 and − 3.88 mm AP from bregma) were processed for TH immunohistochemistry. the brain slices were incubated with 3 % H2O2, blocked with 10 % goat serum, and incubated with primary antibodies overnight at 4 ◦ C. The signals were detected with a DAB staining kit (KeyGEN, China). .. For the quantification of TH + cell numbers in each group, TH + cells in SNpc were conducted at regular, predetermined intervals using a counting frame size of 80 μm × 80 μm and a sampling grid spacing of 100 μm × 100 μm in a Leica DM6 photomicroscope equipped with a digital camera and StereoInvestigator software [30].

other:

Article Title: PCIF1 modulates glioblastoma cell migration and invasion by altering PI(3,4)P2 levels through the PI5-phosphatase INPP5B.
Article Snippet: After primary antibody incubation, all sections were processed using an ABC kit (Vector Laboratories, Burlingame, USA) following the manufacturer’s protocol.

Immunohistochemistry:

Article Title: Cellular fibronectin exacerbates α-synuclein aggregation via integrin alpha4beta1 mediated PARP1 and SCD elevation.
Article Snippet: The sections were then incubated with donkey anti-rabbit IgG (Alexa Fluor 488, Abcam, Catalogue number ab150073, 1:200 dilution) and/or donkey anti-mouse IgG (Alexa Fluor 594, Abcam, Catalogue number ab150108, 1:200 dilution) secondary antibodies, and the nuclei were counterstained with DAPI solution. .. For immunohistochemistry (IHC), the thickness of the brain paraffin section was 40 μm, four sections through the substantia nigra within the range covering the entire SNpc (between − 2.8 and − 3.88 mm AP from bregma) were processed for TH immunohistochemistry. the brain slices were incubated with 3 % H2O2, blocked with 10 % goat serum, and incubated with primary antibodies overnight at 4 ◦ C. The signals were detected with a DAB staining kit (KeyGEN, China). .. For the quantification of TH + cell numbers in each group, TH + cells in SNpc were conducted at regular, predetermined intervals using a counting frame size of 80 μm × 80 μm and a sampling grid spacing of 100 μm × 100 μm in a Leica DM6 photomicroscope equipped with a digital camera and StereoInvestigator software [30].

Paraffin Section:

Article Title: Cellular fibronectin exacerbates α-synuclein aggregation via integrin alpha4beta1 mediated PARP1 and SCD elevation.
Article Snippet: The sections were then incubated with donkey anti-rabbit IgG (Alexa Fluor 488, Abcam, Catalogue number ab150073, 1:200 dilution) and/or donkey anti-mouse IgG (Alexa Fluor 594, Abcam, Catalogue number ab150108, 1:200 dilution) secondary antibodies, and the nuclei were counterstained with DAPI solution. .. For immunohistochemistry (IHC), the thickness of the brain paraffin section was 40 μm, four sections through the substantia nigra within the range covering the entire SNpc (between − 2.8 and − 3.88 mm AP from bregma) were processed for TH immunohistochemistry. the brain slices were incubated with 3 % H2O2, blocked with 10 % goat serum, and incubated with primary antibodies overnight at 4 ◦ C. The signals were detected with a DAB staining kit (KeyGEN, China). .. For the quantification of TH + cell numbers in each group, TH + cells in SNpc were conducted at regular, predetermined intervals using a counting frame size of 80 μm × 80 μm and a sampling grid spacing of 100 μm × 100 μm in a Leica DM6 photomicroscope equipped with a digital camera and StereoInvestigator software [30].

Light Microscopy:

Article Title: Gene regulatory networks orchestrating oocyte fate bifurcation in primordial follicles revealed by single-cell transcriptomics.
Article Snippet: Following washing in PBS, sections were incubated with horseradish peroxidase (HRP)conjugated goat anti‐rabbit IgG (ABclonal, AS014) for 1 hour at room temperature. .. The chromogenic reaction was developed using DAB peroxidase substrate (KeyGEN, KGC3201), and sections were counterstained with hematoxylin (Sigma, H9627-25G) before examination under a light microscope. .. Mouse monoclonal anti-MVH antibody (ab27591) was purchased from Abcam (UK).



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