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ccplus elisa  (Euro Diagnostica)

 
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    Euro Diagnostica ccplus elisa
    Ccplus Elisa, supplied by Euro Diagnostica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom-made+multiplex+microarray/ccplus++elisa/pmc10234203-100-10-12
    Average 90 stars, based on 1 article reviews
    ccplus elisa - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Immunopeptidomics:

    Article Title: Antibody-induced pain-like behavior and bone erosion: links to subclinical inflammation, osteoclast activity, and acid-sensing ion channel 3-dependent sensitization.
    Article Snippet: .. Both patients were carrying Major Histocompatibility Complex, Class II, DR Alpha (HLA-DR) shared epitope allele and were seropositive, with high anticyclic citrullinated antibodies (CCP) levels measured by CCPlus (Euro Diagnostica, Malmö, Sweden). .. Monoclonal antibodies were generated from single memory B cells54 (1103:01B02 and control mAbs 1362:01E02 and 1276: 01G09) or antibody-secreting cells (1325:01B09) isolated from synovial fluid mononuclear cells from the patients with RA.86 After isolation, Ig variable regions were cloned into vectors and mAbs were expressed as recombinant immunoglobulin G (IgG) in Expi293 human embryonic kidney (HEK) cells (Life Technologies, Carlsbad, CA).

    other:

    Article Title: Abstracts from the Focused Nordic Conference Novel Drugs and Drug Targets to Treat Inflammation
    Article Snippet: S FROM THE FOCUSED NORDIC CONFERENCE NOVEL DRUGS AND DRUG TARGETS TO TREAT INFLAMMATION NOVEL THIAZOLINONE AND IMIDAZO-1,2[A]PYRIDINEBASED 5-LIPOXYGENASE INHIBITORS Bettina H., Carmen R., Joanna W., Andreas L., Estella B., Proschak E., Holger S., Dieter S. Goethe University Frankfurt, Institute of Pharmaceutical Chemistry, Frankfurt, Germany Objectives: 5-Lipoxygenase (5-LO) is a crucial enzyme of the arachidonic acid (AA) cascade and catalyses the formation of bioactive leukotrienes (LTs).. LTs are inflammatory mediators playing a pathophysiological role in different diseases like asthma, allergic rhinitis, as well as cardiovascular diseases and certain types of cancer.. Up to now, zileuton is the only 5-LO inhibitor that reached the market.

    Article Title: CIGB-814, an altered peptide ligand derived from human heat-shock protein 60, decreases anti-cyclic citrullinated peptides antibodies in patients with rheumatoid arthritis.
    Article Snippet: Rheumatoid arthritis (RA) is a chronic T cell-mediated autoimmune disease.. Serum autoantibodies against cyclic citrullinated peptides (anti-CCP) are significant markers for diagnosis and prognosis of this disease.. Induction of immune tolerance as therapeutic approach for RA constitutes a current research focal point.

    Article Title: Minor Genetic Overlap Among Rheumatoid Arthritis, Myocardial Infarction, and Myocardial Infarction Risk Determinants.
    Article Snippet: In the EIRA, anti– citrullinated peptide antibody (ACPA) status was determined by screening baseline sera for IgG anti–cyclic citrullinated peptide (CCP) 2 using CCPlus enzyme-linked immunosorbent assay (Euro-Diagnostica) according to the manufacturer’s instructions.


    Article Title: Genome-wide investigation of persistence with methotrexate treatment in early rheumatoid arthritis
    Article Snippet: In EIRA, ACPA status was determined by screening of baseline sera for IgG anti-CCP2 using CCPlus ® ELISA (Euro-Diagnostica AB, Malmö, Sweden) according to the manufacturer’s instructions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Genome-wide investigation of persistence to treatment with methotrexate in early rheumatoid arthritis.
    Article Snippet: .. In EIRA, ACPA status was determined by screening of baseline sera for IgG anti-CCP2 using CCPlusV R ELISA (Euro-Diagnostica AB, Malmö, Sweden) according to the manufacturer’s instructions. ..

    Article Title: The association between autoantibodies and risk for venous thromboembolic events among patients with rheumatoid arthritis
    Article Snippet: .. We centrally screened baseline sera for IgG anti-CCP2 using the CCPlus ELISA (Euro-Diagnostica AB, Malmö, Sweden) according to manufacturer’s instructions, IgA anti-CCP2 using an EliA immunoassay (Phadia AB, Uppsala, Sweden) as previously described [ ], 20 different ACPA fine-specificities ( , available at Rheumatology online), using a custom-made multiplex solid phase microarray platform (Thermo Fisher Scientific, ImmunoDiagnostics, Sweden), as previously described [ ], and IgA, IgG and IgM RF isotypes, using an EliA immunoassay (Phadia), as previously reported [ ]. ..

    Multiplex Assay:

    Article Title: The association between autoantibodies and risk for venous thromboembolic events among patients with rheumatoid arthritis
    Article Snippet: .. We centrally screened baseline sera for IgG anti-CCP2 using the CCPlus ELISA (Euro-Diagnostica AB, Malmö, Sweden) according to manufacturer’s instructions, IgA anti-CCP2 using an EliA immunoassay (Phadia AB, Uppsala, Sweden) as previously described [ ], 20 different ACPA fine-specificities ( , available at Rheumatology online), using a custom-made multiplex solid phase microarray platform (Thermo Fisher Scientific, ImmunoDiagnostics, Sweden), as previously described [ ], and IgA, IgG and IgM RF isotypes, using an EliA immunoassay (Phadia), as previously reported [ ]. ..

    Microarray:

    Article Title: The association between autoantibodies and risk for venous thromboembolic events among patients with rheumatoid arthritis
    Article Snippet: .. We centrally screened baseline sera for IgG anti-CCP2 using the CCPlus ELISA (Euro-Diagnostica AB, Malmö, Sweden) according to manufacturer’s instructions, IgA anti-CCP2 using an EliA immunoassay (Phadia AB, Uppsala, Sweden) as previously described [ ], 20 different ACPA fine-specificities ( , available at Rheumatology online), using a custom-made multiplex solid phase microarray platform (Thermo Fisher Scientific, ImmunoDiagnostics, Sweden), as previously described [ ], and IgA, IgG and IgM RF isotypes, using an EliA immunoassay (Phadia), as previously reported [ ]. ..



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    The anti-CPP3 antibody response in relation to the autoimmune ACPA response. (A) Anti-CPP3 (top panel) and anti-RPP3 (bottom panel) IgG levels in EIRA RA patients (n=2,859) and controls (n=372), measured by multiplex <t>microarray;</t> 98 th percentile cut-off (red line), and 80 th percentile cut-off (blue line) are shown; p-values indicate differences in CPP3/RPP3 IgG levels between groups. (B) Pie chart illustrating the frequency (%) of CPP3+ RA patients (98 th percentile cut-off) in relation to CCP2 status (n=2,859) (top panel), and co-occurrence of anti-CPP3 IgG (98 th percentile cut-off) in relation to eight autoimmune ACPA fine-specificities (measured by multiplex microarray) in EIRA RA patients (n=2,859) (bottom panel); correlation between antibody expression vectors (corrplot package v. 0.77) was used to illustrate co-occurrence, colours signify degree of correlation according to Pearson correlation coefficient (full scale shown on the right). (C) CPP3/RPP3 reactivity in a pool of affinity-purified anti-CCP2 IgG (top panel) and corresponding anti-CCP2 IgG-depleted IgG pool (bottom panel), at different IgG concentrations. AU, arbitrary units; CPP3, citrullinated P. PAD peptide; RPP3, arginine-containing version of CPP3; RA, rheumatoid arthritis; CCP2, cyclic citrullinated peptide(s); OD, optical density; cfc1-cyc, cyclic citrullinated filaggrin peptide 1; Cit-Vim, citrullinated vimentin; Cit-Fib, citrullinated fibrinogen; CEP-1, citrullinated α-enolase peptide 1; Cit-C1, citrullinated triple helical C1 epitope on collagen type II.
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    Image Search Results


    The anti-CPP3 antibody response in relation to the autoimmune ACPA response. (A) Anti-CPP3 (top panel) and anti-RPP3 (bottom panel) IgG levels in EIRA RA patients (n=2,859) and controls (n=372), measured by multiplex microarray; 98 th percentile cut-off (red line), and 80 th percentile cut-off (blue line) are shown; p-values indicate differences in CPP3/RPP3 IgG levels between groups. (B) Pie chart illustrating the frequency (%) of CPP3+ RA patients (98 th percentile cut-off) in relation to CCP2 status (n=2,859) (top panel), and co-occurrence of anti-CPP3 IgG (98 th percentile cut-off) in relation to eight autoimmune ACPA fine-specificities (measured by multiplex microarray) in EIRA RA patients (n=2,859) (bottom panel); correlation between antibody expression vectors (corrplot package v. 0.77) was used to illustrate co-occurrence, colours signify degree of correlation according to Pearson correlation coefficient (full scale shown on the right). (C) CPP3/RPP3 reactivity in a pool of affinity-purified anti-CCP2 IgG (top panel) and corresponding anti-CCP2 IgG-depleted IgG pool (bottom panel), at different IgG concentrations. AU, arbitrary units; CPP3, citrullinated P. PAD peptide; RPP3, arginine-containing version of CPP3; RA, rheumatoid arthritis; CCP2, cyclic citrullinated peptide(s); OD, optical density; cfc1-cyc, cyclic citrullinated filaggrin peptide 1; Cit-Vim, citrullinated vimentin; Cit-Fib, citrullinated fibrinogen; CEP-1, citrullinated α-enolase peptide 1; Cit-C1, citrullinated triple helical C1 epitope on collagen type II.

    Journal: Frontiers in Immunology

    Article Title: Antibodies to a Citrullinated Porphyromonas gingivalis Epitope Are Increased in Early Rheumatoid Arthritis, and Can Be Produced by Gingival Tissue B Cells: Implications for a Bacterial Origin in RA Etiology

    doi: 10.3389/fimmu.2022.804822

    Figure Lengend Snippet: The anti-CPP3 antibody response in relation to the autoimmune ACPA response. (A) Anti-CPP3 (top panel) and anti-RPP3 (bottom panel) IgG levels in EIRA RA patients (n=2,859) and controls (n=372), measured by multiplex microarray; 98 th percentile cut-off (red line), and 80 th percentile cut-off (blue line) are shown; p-values indicate differences in CPP3/RPP3 IgG levels between groups. (B) Pie chart illustrating the frequency (%) of CPP3+ RA patients (98 th percentile cut-off) in relation to CCP2 status (n=2,859) (top panel), and co-occurrence of anti-CPP3 IgG (98 th percentile cut-off) in relation to eight autoimmune ACPA fine-specificities (measured by multiplex microarray) in EIRA RA patients (n=2,859) (bottom panel); correlation between antibody expression vectors (corrplot package v. 0.77) was used to illustrate co-occurrence, colours signify degree of correlation according to Pearson correlation coefficient (full scale shown on the right). (C) CPP3/RPP3 reactivity in a pool of affinity-purified anti-CCP2 IgG (top panel) and corresponding anti-CCP2 IgG-depleted IgG pool (bottom panel), at different IgG concentrations. AU, arbitrary units; CPP3, citrullinated P. PAD peptide; RPP3, arginine-containing version of CPP3; RA, rheumatoid arthritis; CCP2, cyclic citrullinated peptide(s); OD, optical density; cfc1-cyc, cyclic citrullinated filaggrin peptide 1; Cit-Vim, citrullinated vimentin; Cit-Fib, citrullinated fibrinogen; CEP-1, citrullinated α-enolase peptide 1; Cit-C1, citrullinated triple helical C1 epitope on collagen type II.

    Article Snippet: Sera from 2,859 RA patients and 372 controls were screened on a custom-made multiplex microarray (ThermoFisher Scientific, ImmunoDiagnostics Division, Uppsala, Sweden) for IgG reactivity against citrullinated peptides derived from Pg PAD (CPP3) and human proteins , as previously described ( , ).

    Techniques: Multiplex Assay, Microarray, Expressing, Affinity Purification