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computer assisted sperm analysis casa image system  (Hamilton Thorne Ltd)

 
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    Hamilton Thorne Ltd computer assisted sperm analysis casa image system
    Computer Assisted Sperm Analysis Casa Image System, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer-assisted+image+analysis/analysis+assisted+computer+sperm/pm41370039-185-45-53
    Average 86 stars, based on 1 article reviews
    computer assisted sperm analysis casa image system - by Bioz Stars, 2026-11
    86/100 stars

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    Article Title: Cryopreservation of flushed epididymal spermatozoa outperforms epididymal tail vitrification in Spix's yellow-toothed cavy (Galea spixii).
    Article Snippet: Sperm kinematic parameters were evaluated using a computer-aided sperm analyzer (CASA) (IVOS 7.4G; Hamilton-Thorne Research, Beverly, MA, USA) with the settings used for the species by Moreira et al. [18].

    Article Title: Effect of Spirulina platensis extract (C-phycocyanin) supplementation to the semen extender on kinematic and oxidative parameters of Marwari stallions during freezing.
    Article Snippet: 1 Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Bikaner, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan 334001, India 2 Equine Production Campus, ICAR-National Research Centre on Equines, Bikaner, Rajasthan 334001, India 3 Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Bikaner, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan 334001, India 4 Department of Livestock Production and Management, College of Veterinary and Animal Science, Bikaner, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan 334001, India Abstract The current research aimed at examining the impact of different quantities of Spirulina platensis extract (C-phycocyanin) supplemented to the semen extender on the post-thaw quality of Marwari stallion spermatozoa.. Semen samples were obtained via an artificial vagina from six fertile stallions and diluted with an extender containing Spirulina platensis extract (C-phycocyanin) at concentrations of 10, 20, and 50 μg/mL, or without Spirulina as a control.. Aliquots of diluted semen were subjected to cooling (4 °C, 120 min) and then frozen (-196 °C, 1 day), after which the kinematic parameters (VAP average path velocity, VCL curvilinear velocity, VSL straight line velocity, STR straightness, and LIN linearity) as well as antioxidant activities of superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) were assessed in cryopreserved spermatozoa.

    Article Title: Palmitoyltransferase DHHC7 mediates protein palmitoylation and is essential for sperm function through the modulation of [Ca 2+ ] i and ROS signaling
    Article Snippet: Sperm motility and hyperactivation were evaluated using a computer-assisted sperm analyzer (CASA; IVOS; Hamilton-Thorne Biosciences, Beverly, MA, United States), as previously described ( ).

    Article Title: Pentoxifylline and EDTA can modulate the capacitation of frozen/thawed donkey sperm.
    Article Snippet: Background: Capacitation is a process in which sperm undergo a series of modifications that allow it to fertilize the oocyte.. Capacitation inducers are agents or substances that promote these changes essential for in vitro

    Article Title: Breed-specific divergence in boar sperm regulatory profiles involves piRNA and mitochondrial small RNAs.
    Article Snippet: The quality analysis was performed by Computer Assisted Semen Analysis (CASA) on IVOS II (Hamilton Thorne, MA, USA) before transportation.

    Analysis:

    Article Title: Aging, inflammation, and oxidative stress converge on semen quality in Oravka roosters
    Article Snippet: Unless stated otherwise, semen aliquots for sperm-cell assays were diluted in Dulbecco's phosphate-buffered saline (D-PBS; Sigma-Aldrich, St. Louis, MO, USA) to the concentration required by the respective assay. .. Sperm motility was evaluated with the computer-assisted sperm analysis (CASA) system (version 14.0 TOX IVOS II, Hamilton-Thorne Biosciences, Beverly, CA, USA). .. For CASA, semen was diluted in d -PBS to a final working concentration of 2 × 10^6 spermatozoa/mL, and 7 μL of the diluted specimen was pipetted into a Makler counting chamber (10 μm depth; Sefi Medical Instruments, Haifa, Israel).



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    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay