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las v4 1 computer based image analysis software  (Danaher Inc)


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    Danaher Inc las v4 1 computer based image analysis software
    Las V4 1 Computer Based Image Analysis Software, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 3750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computation+and+analysis+software/LAS+X+Life+Science+Microscope+Software+Platform/pm40629482-72-26-32
    Average 99 stars, based on 3750 article reviews
    las v4 1 computer based image analysis software - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Confocal:

    Article Title: A Hypothalamic Pathway for Energy Conservation
    Article Snippet: .. For confocal microscopy, we used Leica LAS X Confocal and the Leica Microscope Platform. ..

    Microscopy:

    Article Title: A Hypothalamic Pathway for Energy Conservation
    Article Snippet: .. For confocal microscopy, we used Leica LAS X Confocal and the Leica Microscope Platform. ..

    Article Title: Opposing roles for lipocalins and a CD36 family scavenger receptor in apical extracellular matrix-dependent protection of narrow tube integrity
    Article Snippet: Epifluorescent and differential interference contrast images of the L1 duct and pore cuticle and adult alae were captured using a Zeiss Axioskop and a Leica DFC360 FX camera. .. Confocal images were captured using a Leica TCS SP8 or Leica TCS DMi8 confocal microscope and Leica Las X Software; accompanying transmitted light images used Dodt gradient contrast or differential interference contrast imaging, respectively. ..

    Article Title: Development-inspired approach for the derivation of functional and transplantable salivary gland organoids from human pluripotent stem cells
    Article Snippet: Images were visualized with Zen Black Edition (Zeiss), or LAS X Life Science (Leica) Microscope Software Platformand then analyzed and quantified using FIJI (ImageJ) software. .. Finally, videos were compiled using either the LAS X Life Science Microscope Software Platform (Leica) or FIJI (ImageJ) software. ..

    Article Title: Engineered 3D Fibronectin‐Based Environments Modulate the Pathogenic Profile of Arthritic Synovial Fibroblasts
    Article Snippet: Samples were treated with the appropriate secondary antibody for 1 h at room temperature and washed with PBS 0.5% Tween 20 before mounting using Vectashield slow-fading media containing DAPI (VectorLabs). .. Scanning was carried out on a Leica DM8 widefield microscope using LAS X Life Science software at a magnification of ×10. ..

    Article Title: The biophysical interaction network that couples zona occludens-1 to membrane proteins at tight junctions
    Article Snippet: Phase separation droplets were visualized using a Leica DM6B upright microscope (Leica Microsystems) and images were captured using 20x, 40x, and 100x objectives under oil. .. LAS X Lifescience Microscope software (Leica Microsystems) was used for image generation. ..

    Software:

    Article Title: Opposing roles for lipocalins and a CD36 family scavenger receptor in apical extracellular matrix-dependent protection of narrow tube integrity
    Article Snippet: Epifluorescent and differential interference contrast images of the L1 duct and pore cuticle and adult alae were captured using a Zeiss Axioskop and a Leica DFC360 FX camera. .. Confocal images were captured using a Leica TCS SP8 or Leica TCS DMi8 confocal microscope and Leica Las X Software; accompanying transmitted light images used Dodt gradient contrast or differential interference contrast imaging, respectively. ..

    Article Title: Development-inspired approach for the derivation of functional and transplantable salivary gland organoids from human pluripotent stem cells
    Article Snippet: Images were visualized with Zen Black Edition (Zeiss), or LAS X Life Science (Leica) Microscope Software Platformand then analyzed and quantified using FIJI (ImageJ) software. .. Finally, videos were compiled using either the LAS X Life Science Microscope Software Platform (Leica) or FIJI (ImageJ) software. ..

    Article Title: Engineered 3D Fibronectin‐Based Environments Modulate the Pathogenic Profile of Arthritic Synovial Fibroblasts
    Article Snippet: Samples were treated with the appropriate secondary antibody for 1 h at room temperature and washed with PBS 0.5% Tween 20 before mounting using Vectashield slow-fading media containing DAPI (VectorLabs). .. Scanning was carried out on a Leica DM8 widefield microscope using LAS X Life Science software at a magnification of ×10. ..

    Article Title: Aluminum facilitates flavonol accumulation to modulate sensitivity thresholds of tea roots to aluminum
    Article Snippet: 498 Calcofluor white staining for cell wall: After the slices of tea roots were stained with calcofluor 499 white stain solution (Coolaber, Beijing, China) for 30 seconds, they were imaged using a laser confocal 500 scanning microscope, with excitation wavelength at 405 nm. .. The quantification of fluorescence intensity 501 values of DPBA staining (flavonols), Morin staining (Al), DAPI staining (nucleus) and Calcofluor white 502 staining (cell wall) was all counted by Leica Application Suite X (LAS X) software platform. ..

    Article Title: The biophysical interaction network that couples zona occludens-1 to membrane proteins at tight junctions
    Article Snippet: Phase separation droplets were visualized using a Leica DM6B upright microscope (Leica Microsystems) and images were captured using 20x, 40x, and 100x objectives under oil. .. LAS X Lifescience Microscope software (Leica Microsystems) was used for image generation. ..

    Imaging:

    Article Title: Opposing roles for lipocalins and a CD36 family scavenger receptor in apical extracellular matrix-dependent protection of narrow tube integrity
    Article Snippet: Epifluorescent and differential interference contrast images of the L1 duct and pore cuticle and adult alae were captured using a Zeiss Axioskop and a Leica DFC360 FX camera. .. Confocal images were captured using a Leica TCS SP8 or Leica TCS DMi8 confocal microscope and Leica Las X Software; accompanying transmitted light images used Dodt gradient contrast or differential interference contrast imaging, respectively. ..

    Article Title: Iturin A disrupts Cryptococcus neoformans membrane integrity, attenuates virulence, and synergizes with amphotericin B
    Article Snippet: .. To validate the fungal membrane permeability after ITA treatment in the presence and absence of ergosterol, C. neoformans cells stained with PI were visualized under a Leica DMi8 inverted microscope at a magnification of ×40, prior to imaging with a Leica DFC7000T digital camera using the Leica software platform LAS X. Untreated and AmpB-treated (2×MIC, 1 μg/mL) cells were used as negative and positive controls, respectively. ..

    Widefield:

    Article Title: Engineered 3D Fibronectin‐Based Environments Modulate the Pathogenic Profile of Arthritic Synovial Fibroblasts
    Article Snippet: Samples were treated with the appropriate secondary antibody for 1 h at room temperature and washed with PBS 0.5% Tween 20 before mounting using Vectashield slow-fading media containing DAPI (VectorLabs). .. Scanning was carried out on a Leica DM8 widefield microscope using LAS X Life Science software at a magnification of ×10. ..

    other:

    Article Title: Bidirectional photoriboswitch for translational regulation in mammalian cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 5′ UTR sequence, see Table S1 Guangzhou Ruibo Biotechnology Co., Ltd. N/A 3′ UTR sequence, see Table S2 Guangzhou Ruibo Biotechnology Co., Ltd. N/A PCR primers Guangzhou Ruibo Biotechnology Co., Ltd. N/A Recombinant DNA Plasmid mCherry-Mito-7 Olenych et al.91 Addgene#55102 Plasmid pEBFP2-Nuc Ai et al.92 Addgene#14893 Plasmid miRFP670nano Oliinyk et al.93 Addgene#127443 Plasmid pCMV-N1-mousesuperfolderGFP (mGRASP) Yamagata et al.71 Addgene#42143 Plasmid pcDNA3.1_pMagFast2 (3x)-iRFP Kawano et al.53 Addgene#67297 Plasmid pcDNA3.1_nMagHigh1-EGFP-CAAX Kawano et al.53 Addgene#67300 Plasmid pSKDuet16 Ellila et al.58 Addgene#41684 Plasmid pETZZtevPP7CP(C68A,C73A) Hogg et al.72 Addgene#27548 Plasmid encoding CRY2 Song et al.54 N/A Plasmid encoding CIBN Song et al.54 N/A Plasmid encoding EGFP Shibata et al.94 N/A Plasmid encoding MS2CP Nakanishi et al.23,51 N/A Software and algorithms Microsoft Excel for Microsoft 365 Microsoft N/A FIJI ImageJ https://fiji.sc/ Pymol Schrödinger, LLC https://www.pymol.org/ GraphPad Prism GraphPad Software https://www.graphpad.com TM-score on-line Zhang Lab, NUS https://aideepmed.com/TM-score/ AlphaFold 3 Google DeepMind and Isomorphic Labs https://alphafoldserver.com/ LAS X Life Science Microscope Software Leica https://www.leica-microsystems.com/products/ microscope-software/p/leica-las-x-ls/ Attune NxT Flow Cytometer Software version 4.2 Thermo Fisher https://www.thermofisher.com/hk/en/home/life- science/cell-analysis/flow-cytometry/flowcytometers/attune-acoustic-focusing-flowcytometer/attune-cytometer-software.html iScience 29, 117671, October 16, 2026 13 commercial repositories, individual informed consent was not applicable.

    Membrane:

    Article Title: Iturin A disrupts Cryptococcus neoformans membrane integrity, attenuates virulence, and synergizes with amphotericin B
    Article Snippet: .. To validate the fungal membrane permeability after ITA treatment in the presence and absence of ergosterol, C. neoformans cells stained with PI were visualized under a Leica DMi8 inverted microscope at a magnification of ×40, prior to imaging with a Leica DFC7000T digital camera using the Leica software platform LAS X. Untreated and AmpB-treated (2×MIC, 1 μg/mL) cells were used as negative and positive controls, respectively. ..

    Permeability:

    Article Title: Iturin A disrupts Cryptococcus neoformans membrane integrity, attenuates virulence, and synergizes with amphotericin B
    Article Snippet: .. To validate the fungal membrane permeability after ITA treatment in the presence and absence of ergosterol, C. neoformans cells stained with PI were visualized under a Leica DMi8 inverted microscope at a magnification of ×40, prior to imaging with a Leica DFC7000T digital camera using the Leica software platform LAS X. Untreated and AmpB-treated (2×MIC, 1 μg/mL) cells were used as negative and positive controls, respectively. ..

    Staining:

    Article Title: Iturin A disrupts Cryptococcus neoformans membrane integrity, attenuates virulence, and synergizes with amphotericin B
    Article Snippet: .. To validate the fungal membrane permeability after ITA treatment in the presence and absence of ergosterol, C. neoformans cells stained with PI were visualized under a Leica DMi8 inverted microscope at a magnification of ×40, prior to imaging with a Leica DFC7000T digital camera using the Leica software platform LAS X. Untreated and AmpB-treated (2×MIC, 1 μg/mL) cells were used as negative and positive controls, respectively. ..

    Article Title: Aluminum facilitates flavonol accumulation to modulate sensitivity thresholds of tea roots to aluminum
    Article Snippet: 498 Calcofluor white staining for cell wall: After the slices of tea roots were stained with calcofluor 499 white stain solution (Coolaber, Beijing, China) for 30 seconds, they were imaged using a laser confocal 500 scanning microscope, with excitation wavelength at 405 nm. .. The quantification of fluorescence intensity 501 values of DPBA staining (flavonols), Morin staining (Al), DAPI staining (nucleus) and Calcofluor white 502 staining (cell wall) was all counted by Leica Application Suite X (LAS X) software platform. ..

    Inverted Microscopy:

    Article Title: Iturin A disrupts Cryptococcus neoformans membrane integrity, attenuates virulence, and synergizes with amphotericin B
    Article Snippet: .. To validate the fungal membrane permeability after ITA treatment in the presence and absence of ergosterol, C. neoformans cells stained with PI were visualized under a Leica DMi8 inverted microscope at a magnification of ×40, prior to imaging with a Leica DFC7000T digital camera using the Leica software platform LAS X. Untreated and AmpB-treated (2×MIC, 1 μg/mL) cells were used as negative and positive controls, respectively. ..

    Fluorescence:

    Article Title: Aluminum facilitates flavonol accumulation to modulate sensitivity thresholds of tea roots to aluminum
    Article Snippet: 498 Calcofluor white staining for cell wall: After the slices of tea roots were stained with calcofluor 499 white stain solution (Coolaber, Beijing, China) for 30 seconds, they were imaged using a laser confocal 500 scanning microscope, with excitation wavelength at 405 nm. .. The quantification of fluorescence intensity 501 values of DPBA staining (flavonols), Morin staining (Al), DAPI staining (nucleus) and Calcofluor white 502 staining (cell wall) was all counted by Leica Application Suite X (LAS X) software platform. ..

    DAPI Staining:

    Article Title: Aluminum facilitates flavonol accumulation to modulate sensitivity thresholds of tea roots to aluminum
    Article Snippet: 498 Calcofluor white staining for cell wall: After the slices of tea roots were stained with calcofluor 499 white stain solution (Coolaber, Beijing, China) for 30 seconds, they were imaged using a laser confocal 500 scanning microscope, with excitation wavelength at 405 nm. .. The quantification of fluorescence intensity 501 values of DPBA staining (flavonols), Morin staining (Al), DAPI staining (nucleus) and Calcofluor white 502 staining (cell wall) was all counted by Leica Application Suite X (LAS X) software platform. ..



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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay