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DIAGENODE DIAGNOSTICS chip lysis buffer
Chip Lysis Buffer, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip+buffer/chip+lysis+buffer/pmc06185902-272-0-32
Average 90 stars, based on 1 article reviews
chip lysis buffer - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Chromatin Immunoprecipitation:

Article Title: The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly.
Article Snippet: .. The lysates were collected, diluted with ChIP lysis buffer, and sonicated with a Bioruptor Pico (Diagenode) for 10 cycles (30s on/30s off) to produce DNA fragments of 100–500 bp in length. .. The cleared lysates were incubated with the following antibodies overnight at 4 ̊C: H3K9me2 (Abcam, 115159), H3K14ac (Upstate, 07–353), and Pol II S2P (Abcam, ab5059).

Article Title: ER and Nutrient Stress Promote Assembly of Respiratory Chain Supercomplexes Through PERK/eIF2α Axis
Article Snippet: A minimum of 1.0×10 7 glucose or galactose-grown cells were crosslinked with 1% formaldehyde (Sigma) for 10 min followed by quenching with 0.125 M glycine for 5 min. .. Cells were lysed with ChIP lysis buffer (50 mM HEPES, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, and Complete protease inhibitors and sonicated in a Diagenode Bioruptor for 5 cycles for 5 min with a duty cycle of 30 s “on” and 30 s “off.” Samples were immunoprecipitated with specific antibodies to anti-ATF4 D4B8 and normal rabbit IgG. ..

Article Title: A Stat1 bound enhancer promotes Nampt expression and function within tumor associated macrophages.
Article Snippet: 5 × 107 RAW 264.7 cells per sample in 15 cm tissue culture plates treated with 50 ng/ml IFNγ or medium were crosslinked for 15 min at 37° in PBS with 5 mM EDTA and 1% paraformaldehyde, quenched with glycine, and lysed at 0° in ChIP Lysis Buffer (FA buffer containing 0.1% NP-40, 0.1% SDS, 2 mM MgCl2, 1 mM DTT, and protease inhibitors, using zircon beads with a Mini-Bead-Beater 96 (Biospec Products)). .. Washed lysate pellets were resuspended in fresh ChIP Lysis Buffer and sonicated using a Bioruptor XL bath sonicator (Diagenode). .. Soluble chromatin containing DNA sheared to 200 bp average length was immunoprecipitated with anti-STAT1 (sc-529, Santa Cruz) bound to Protein-G magnetic beads81.

Article Title: Genome-wide analysis of Pax8 binding provides new insights into thyroid functions
Article Snippet: .. The cell pellet was resuspended consecutively in ChIP lysis buffers [ ] and sonicated for 90 minutes (30 seconds high frequency pulsing followed by 30 seconds resting) using the Bioruptor sonicator (Diagenode, Denville, NJ) to produce chromatin fragments of 200–500 bp on average. .. The cell pellet was resuspended consecutively in ChIP lysis buffers [ ] and sonicated for 90 minutes (30 seconds high frequency pulsing followed by 30 seconds resting) using the Bioruptor sonicator (Diagenode, Denville, NJ) to produce chromatin fragments of 200–500 bp on average.

Article Title: STAT3 Signaling Induces the Differentiation of Human ICOS + CD4 T Cells Helping B lymphocytes
Article Snippet: .. Cells were resuspended in ChIP lysis buffer and subjected to sonication (40 cycles of 30 sec) using a bioruptor device (Diagenode, Liège, Belgium) to obtain DNA fragments ranging from 150 to 500 bp. .. Cells were resuspended in ChIP lysis buffer and subjected to sonication (40 cycles of 30 sec) using a bioruptor device (Diagenode, Liège, Belgium) to obtain DNA fragments ranging from 150 to 500 bp.

Article Title: Diabetes-induced lncRNA Dnm3os regulates macrophage functions and inflammation via nuclear mechanisms
Article Snippet: Briefly, RAW macrophages were crosslinked with 1% formaldehyde (Cat. No. F8775, Sigma Aldrich) for 10 min at room temperature, quenched with 0.125M glycine for 5 min, and washed with ice-cold PBS. .. Cells were lysed in ChIP lysis buffer and chromatin was sheared (upto 200–600 bp) by sonication (8 cycles, 30 sec on/30 sec off) using Bioruptor R Pico (Diagenode, Denville, NJ). .. Immunoprecipitation was performed using 3 μg of antibodies specific to H3K9ac (Cat. Nos. ab10812, lot no. GR143846-2; Abcam, Cambridge, UK), or control rabbit IgG, overnight at 4°C.

Article Title: Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1
Article Snippet: .. The nuclei were resuspended in ChIP lysis buffer (20 mM Tris, pH 7.4; 150 mM NaCl; 1% sodium deoxycholate; 0.1% SDS; 1 mM EDTA, pH 8.0) and sonicated using a Bioruptor Pico sonicator (Diagenode) with the following settings: 30 s ON/OFF, 20 cycles. .. The sonicated chromatin was quality controlled using the Agilent 2100 Bioanalyzer system.

Lysis:

Article Title: The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly.
Article Snippet: .. The lysates were collected, diluted with ChIP lysis buffer, and sonicated with a Bioruptor Pico (Diagenode) for 10 cycles (30s on/30s off) to produce DNA fragments of 100–500 bp in length. .. The cleared lysates were incubated with the following antibodies overnight at 4 ̊C: H3K9me2 (Abcam, 115159), H3K14ac (Upstate, 07–353), and Pol II S2P (Abcam, ab5059).

Article Title: ER and Nutrient Stress Promote Assembly of Respiratory Chain Supercomplexes Through PERK/eIF2α Axis
Article Snippet: A minimum of 1.0×10 7 glucose or galactose-grown cells were crosslinked with 1% formaldehyde (Sigma) for 10 min followed by quenching with 0.125 M glycine for 5 min. .. Cells were lysed with ChIP lysis buffer (50 mM HEPES, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, and Complete protease inhibitors and sonicated in a Diagenode Bioruptor for 5 cycles for 5 min with a duty cycle of 30 s “on” and 30 s “off.” Samples were immunoprecipitated with specific antibodies to anti-ATF4 D4B8 and normal rabbit IgG. ..

Article Title: A Stat1 bound enhancer promotes Nampt expression and function within tumor associated macrophages.
Article Snippet: 5 × 107 RAW 264.7 cells per sample in 15 cm tissue culture plates treated with 50 ng/ml IFNγ or medium were crosslinked for 15 min at 37° in PBS with 5 mM EDTA and 1% paraformaldehyde, quenched with glycine, and lysed at 0° in ChIP Lysis Buffer (FA buffer containing 0.1% NP-40, 0.1% SDS, 2 mM MgCl2, 1 mM DTT, and protease inhibitors, using zircon beads with a Mini-Bead-Beater 96 (Biospec Products)). .. Washed lysate pellets were resuspended in fresh ChIP Lysis Buffer and sonicated using a Bioruptor XL bath sonicator (Diagenode). .. Soluble chromatin containing DNA sheared to 200 bp average length was immunoprecipitated with anti-STAT1 (sc-529, Santa Cruz) bound to Protein-G magnetic beads81.

Article Title: Genome-wide analysis of Pax8 binding provides new insights into thyroid functions
Article Snippet: .. The cell pellet was resuspended consecutively in ChIP lysis buffers [ ] and sonicated for 90 minutes (30 seconds high frequency pulsing followed by 30 seconds resting) using the Bioruptor sonicator (Diagenode, Denville, NJ) to produce chromatin fragments of 200–500 bp on average. .. The cell pellet was resuspended consecutively in ChIP lysis buffers [ ] and sonicated for 90 minutes (30 seconds high frequency pulsing followed by 30 seconds resting) using the Bioruptor sonicator (Diagenode, Denville, NJ) to produce chromatin fragments of 200–500 bp on average.

Article Title: STAT3 Signaling Induces the Differentiation of Human ICOS + CD4 T Cells Helping B lymphocytes
Article Snippet: .. Cells were resuspended in ChIP lysis buffer and subjected to sonication (40 cycles of 30 sec) using a bioruptor device (Diagenode, Liège, Belgium) to obtain DNA fragments ranging from 150 to 500 bp. .. Cells were resuspended in ChIP lysis buffer and subjected to sonication (40 cycles of 30 sec) using a bioruptor device (Diagenode, Liège, Belgium) to obtain DNA fragments ranging from 150 to 500 bp.

Article Title: Diabetes-induced lncRNA Dnm3os regulates macrophage functions and inflammation via nuclear mechanisms
Article Snippet: Briefly, RAW macrophages were crosslinked with 1% formaldehyde (Cat. No. F8775, Sigma Aldrich) for 10 min at room temperature, quenched with 0.125M glycine for 5 min, and washed with ice-cold PBS. .. Cells were lysed in ChIP lysis buffer and chromatin was sheared (upto 200–600 bp) by sonication (8 cycles, 30 sec on/30 sec off) using Bioruptor R Pico (Diagenode, Denville, NJ). .. Immunoprecipitation was performed using 3 μg of antibodies specific to H3K9ac (Cat. Nos. ab10812, lot no. GR143846-2; Abcam, Cambridge, UK), or control rabbit IgG, overnight at 4°C.

Article Title: Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1
Article Snippet: .. The nuclei were resuspended in ChIP lysis buffer (20 mM Tris, pH 7.4; 150 mM NaCl; 1% sodium deoxycholate; 0.1% SDS; 1 mM EDTA, pH 8.0) and sonicated using a Bioruptor Pico sonicator (Diagenode) with the following settings: 30 s ON/OFF, 20 cycles. .. The sonicated chromatin was quality controlled using the Agilent 2100 Bioanalyzer system.

Sonication:

Article Title: The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly.
Article Snippet: .. The lysates were collected, diluted with ChIP lysis buffer, and sonicated with a Bioruptor Pico (Diagenode) for 10 cycles (30s on/30s off) to produce DNA fragments of 100–500 bp in length. .. The cleared lysates were incubated with the following antibodies overnight at 4 ̊C: H3K9me2 (Abcam, 115159), H3K14ac (Upstate, 07–353), and Pol II S2P (Abcam, ab5059).

Article Title: ER and Nutrient Stress Promote Assembly of Respiratory Chain Supercomplexes Through PERK/eIF2α Axis
Article Snippet: A minimum of 1.0×10 7 glucose or galactose-grown cells were crosslinked with 1% formaldehyde (Sigma) for 10 min followed by quenching with 0.125 M glycine for 5 min. .. Cells were lysed with ChIP lysis buffer (50 mM HEPES, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, and Complete protease inhibitors and sonicated in a Diagenode Bioruptor for 5 cycles for 5 min with a duty cycle of 30 s “on” and 30 s “off.” Samples were immunoprecipitated with specific antibodies to anti-ATF4 D4B8 and normal rabbit IgG. ..

Article Title: A Stat1 bound enhancer promotes Nampt expression and function within tumor associated macrophages.
Article Snippet: 5 × 107 RAW 264.7 cells per sample in 15 cm tissue culture plates treated with 50 ng/ml IFNγ or medium were crosslinked for 15 min at 37° in PBS with 5 mM EDTA and 1% paraformaldehyde, quenched with glycine, and lysed at 0° in ChIP Lysis Buffer (FA buffer containing 0.1% NP-40, 0.1% SDS, 2 mM MgCl2, 1 mM DTT, and protease inhibitors, using zircon beads with a Mini-Bead-Beater 96 (Biospec Products)). .. Washed lysate pellets were resuspended in fresh ChIP Lysis Buffer and sonicated using a Bioruptor XL bath sonicator (Diagenode). .. Soluble chromatin containing DNA sheared to 200 bp average length was immunoprecipitated with anti-STAT1 (sc-529, Santa Cruz) bound to Protein-G magnetic beads81.

Article Title: Genome-wide analysis of Pax8 binding provides new insights into thyroid functions
Article Snippet: .. The cell pellet was resuspended consecutively in ChIP lysis buffers [ ] and sonicated for 90 minutes (30 seconds high frequency pulsing followed by 30 seconds resting) using the Bioruptor sonicator (Diagenode, Denville, NJ) to produce chromatin fragments of 200–500 bp on average. .. The cell pellet was resuspended consecutively in ChIP lysis buffers [ ] and sonicated for 90 minutes (30 seconds high frequency pulsing followed by 30 seconds resting) using the Bioruptor sonicator (Diagenode, Denville, NJ) to produce chromatin fragments of 200–500 bp on average.

Article Title: STAT3 Signaling Induces the Differentiation of Human ICOS + CD4 T Cells Helping B lymphocytes
Article Snippet: .. Cells were resuspended in ChIP lysis buffer and subjected to sonication (40 cycles of 30 sec) using a bioruptor device (Diagenode, Liège, Belgium) to obtain DNA fragments ranging from 150 to 500 bp. .. Cells were resuspended in ChIP lysis buffer and subjected to sonication (40 cycles of 30 sec) using a bioruptor device (Diagenode, Liège, Belgium) to obtain DNA fragments ranging from 150 to 500 bp.

Article Title: Diabetes-induced lncRNA Dnm3os regulates macrophage functions and inflammation via nuclear mechanisms
Article Snippet: Briefly, RAW macrophages were crosslinked with 1% formaldehyde (Cat. No. F8775, Sigma Aldrich) for 10 min at room temperature, quenched with 0.125M glycine for 5 min, and washed with ice-cold PBS. .. Cells were lysed in ChIP lysis buffer and chromatin was sheared (upto 200–600 bp) by sonication (8 cycles, 30 sec on/30 sec off) using Bioruptor R Pico (Diagenode, Denville, NJ). .. Immunoprecipitation was performed using 3 μg of antibodies specific to H3K9ac (Cat. Nos. ab10812, lot no. GR143846-2; Abcam, Cambridge, UK), or control rabbit IgG, overnight at 4°C.

Article Title: Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1
Article Snippet: .. The nuclei were resuspended in ChIP lysis buffer (20 mM Tris, pH 7.4; 150 mM NaCl; 1% sodium deoxycholate; 0.1% SDS; 1 mM EDTA, pH 8.0) and sonicated using a Bioruptor Pico sonicator (Diagenode) with the following settings: 30 s ON/OFF, 20 cycles. .. The sonicated chromatin was quality controlled using the Agilent 2100 Bioanalyzer system.

other:

Article Title: Loss of TP63 promotes the metastasis of head and neck squamous cell carcinoma by activating MAPK and STAT3 signaling
Article Snippet: After the second wash, cells were lysed using ChIP-SDS lysis buffer (1% SDS, 10mM EDTS, 50mM Tris-HCl, pH 8.1, protease inhibitor cocktail (Roche, Basel, Switzerland)).

Immunoprecipitation:

Article Title: ER and Nutrient Stress Promote Assembly of Respiratory Chain Supercomplexes Through PERK/eIF2α Axis
Article Snippet: A minimum of 1.0×10 7 glucose or galactose-grown cells were crosslinked with 1% formaldehyde (Sigma) for 10 min followed by quenching with 0.125 M glycine for 5 min. .. Cells were lysed with ChIP lysis buffer (50 mM HEPES, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, and Complete protease inhibitors and sonicated in a Diagenode Bioruptor for 5 cycles for 5 min with a duty cycle of 30 s “on” and 30 s “off.” Samples were immunoprecipitated with specific antibodies to anti-ATF4 D4B8 and normal rabbit IgG. ..



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