Review




Structured Review

nanoAnalytics gmbh cellzscope software
Stable expression of E7 in MDCK monolayers induces the development of a higher peak of TER and changes the expression of claudins. (A) Parental MDCK and MDCK-E7 monolayers plated in Transwell inserts were subjected to a Ca-switch and the development of TER was measured using the automated cell monitoring system, <t>cellZscope.</t> Data are presented the mean ± SD from three independent monolayers per condition plated on Transwell inserts. Statistical analysis was performed at the 18 h with Student's t-test. **** P<0.0001. (B) Immunofluorescence detection of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative images from three independent experiments. Scale bar, 10 µ m. (C) Mean fluorescence intensity measurements of Cl-1, -2, -4 and -10 performed at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Data derived from three independent images from each condition and from three independent experiments. Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. **** P<0.0001. (D) Western blot analysis of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative western blots of at least three independent experiments (left panel) and the densitometry analysis (right panel). Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. * P<0.05, ** P<0.01, **** P<0.0001. Ca, calcium; Cl, claudin; TER, transepithelial electrical resistance; MDCK, Madin-Darby Canine Kidney; MDCK-E7, MDCK cells transfected with E7 from HPV; HPV, human papillomavirus.
Cellzscope Software, supplied by nanoAnalytics gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellzscope/cellzscope+software/pmc07473757-98-6-10
Average 90 stars, based on 1 article reviews
cellzscope software - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions"

Article Title: E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions

Journal: International Journal of Oncology

doi: 10.3892/ijo.2020.5105

Stable expression of E7 in MDCK monolayers induces the development of a higher peak of TER and changes the expression of claudins. (A) Parental MDCK and MDCK-E7 monolayers plated in Transwell inserts were subjected to a Ca-switch and the development of TER was measured using the automated cell monitoring system, cellZscope. Data are presented the mean ± SD from three independent monolayers per condition plated on Transwell inserts. Statistical analysis was performed at the 18 h with Student's t-test. **** P<0.0001. (B) Immunofluorescence detection of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative images from three independent experiments. Scale bar, 10 µ m. (C) Mean fluorescence intensity measurements of Cl-1, -2, -4 and -10 performed at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Data derived from three independent images from each condition and from three independent experiments. Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. **** P<0.0001. (D) Western blot analysis of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative western blots of at least three independent experiments (left panel) and the densitometry analysis (right panel). Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. * P<0.05, ** P<0.01, **** P<0.0001. Ca, calcium; Cl, claudin; TER, transepithelial electrical resistance; MDCK, Madin-Darby Canine Kidney; MDCK-E7, MDCK cells transfected with E7 from HPV; HPV, human papillomavirus.
Figure Legend Snippet: Stable expression of E7 in MDCK monolayers induces the development of a higher peak of TER and changes the expression of claudins. (A) Parental MDCK and MDCK-E7 monolayers plated in Transwell inserts were subjected to a Ca-switch and the development of TER was measured using the automated cell monitoring system, cellZscope. Data are presented the mean ± SD from three independent monolayers per condition plated on Transwell inserts. Statistical analysis was performed at the 18 h with Student's t-test. **** P<0.0001. (B) Immunofluorescence detection of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative images from three independent experiments. Scale bar, 10 µ m. (C) Mean fluorescence intensity measurements of Cl-1, -2, -4 and -10 performed at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Data derived from three independent images from each condition and from three independent experiments. Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. **** P<0.0001. (D) Western blot analysis of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative western blots of at least three independent experiments (left panel) and the densitometry analysis (right panel). Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. * P<0.05, ** P<0.01, **** P<0.0001. Ca, calcium; Cl, claudin; TER, transepithelial electrical resistance; MDCK, Madin-Darby Canine Kidney; MDCK-E7, MDCK cells transfected with E7 from HPV; HPV, human papillomavirus.

Techniques Used: Expressing, Immunofluorescence, Fluorescence, Derivative Assay, Standard Deviation, Western Blot, Transfection

Related Articles

Control:

Article Title: An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells
Article Snippet: The transwell inserts have a transparent PET membrane with a pore size of 0.4 μM (BD Falcon, Corning). .. After the establishment of a barrier of at least 700 Ωcm 2 (after 5 days), cells were treated with adhibin (2 μM, 10 μM, 25 μM, or 40 μM) or 0.3% DMSO (control) and TEER was monitored every hour for 48 h. The TEER data were automatically recorded by the cellZscope software (nanoAnalytics). ..

Article Title: An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells.
Article Snippet: The transwell inserts have a transparent PET membrane with a pore size of 0.4 μM (BD Falcon, Corning). .. After the establishment of a barrier of at least 700 Ωcm2 (after 5 days), cells were treated with adhibin (2 μM, 10 μM, 25 μM, or 40 μM) or 0.3% DMSO (control) and TEER was monitored every hour for 48 h. The TEER data were automatically recorded by the cellZscope software (nanoAnalytics). ..

Software:

Article Title: An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells
Article Snippet: The transwell inserts have a transparent PET membrane with a pore size of 0.4 μM (BD Falcon, Corning). .. After the establishment of a barrier of at least 700 Ωcm 2 (after 5 days), cells were treated with adhibin (2 μM, 10 μM, 25 μM, or 40 μM) or 0.3% DMSO (control) and TEER was monitored every hour for 48 h. The TEER data were automatically recorded by the cellZscope software (nanoAnalytics). ..

Article Title: A Novel Impedance Platform Based on Printed Polymer Electrodes for Automated Virus Neutralization Assays
Article Snippet: .. An external controller transmits the signals to a computer (Figure 1D) running a tailored version of the cellZscope software (nanoAnalytics GmbH) for data acquisition and analysis (Figure 1E), developed on the basis of the programming environment Visual Studio. ..

Article Title: Effects of small extracellular vesicles derived from normoxia- and hypoxia-treated prostate cancer cells on the submandibular salivary gland epithelium in vitro .
Article Snippet: .. Analyses of automated cell monitoring (TER, CAP) were done with the CellZScope software (Version 2.2.4, nanoAnalytics GmbH). .. Plots and statistical analyses were done with GraphPad Prism 8.0 (GraphPad Software, USA) and Microsoft Excel.

Article Title: Effects of small extracellular vesicles derived from normoxia- and hypoxia-treated prostate cancer cells on the submandibular salivary gland epithelium in vitro .
Article Snippet: Basolateral media were either serum-free, with 10% FBS or 10% sEVdepleted FBS in condition optimization experiments and serum-free or 10% FBS when sEVs were added in physiological concentrations.38 Small EV depleted FBS was prepared by ultrafiltration, using Amicon15 ultrafiltering tubes (UFC810096, Sigma-Aldrich). .. For the final TER [Ω x cm2] and CAP values [μF/ cm2] blank inserts (without cells) were subtracted for each time point, and values were calculated according to the algorithm of the CellZscope Software (Version 2.2.4, nanoAnalytics GmbH). .. DU145, human prostate adenocarcinoma cells were maintained in DMEM (Sigma, D5796) supplemented with 10% FBS, 1% Sodium pyruvate solution (S8636, Merck Life) and 1% Pen/strep.

Article Title: An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells.
Article Snippet: The transwell inserts have a transparent PET membrane with a pore size of 0.4 μM (BD Falcon, Corning). .. After the establishment of a barrier of at least 700 Ωcm2 (after 5 days), cells were treated with adhibin (2 μM, 10 μM, 25 μM, or 40 μM) or 0.3% DMSO (control) and TEER was monitored every hour for 48 h. The TEER data were automatically recorded by the cellZscope software (nanoAnalytics). ..

Article Title: Effects of small extracellular vesicles derived from normoxia- and hypoxia-treated prostate cancer cells on the submandibular salivary gland epithelium in vitro
Article Snippet: Small EV depleted FBS was prepared by ultrafiltration, using Amicon-15 ultrafiltering tubes (UFC810096, Sigma-Aldrich). .. For the final TER [Ω x cm 2 ] and CAP values [μF/cm 2 ] blank inserts (without cells) were subtracted for each time point, and values were calculated according to the algorithm of the CellZscope Software (Version 2.2.4, nanoAnalytics GmbH). .. DU145, human prostate adenocarcinoma cells were maintained in DMEM (Sigma, D5796) supplemented with 10% FBS, 1% Sodium pyruvate solution (S8636, Merck Life) and 1% Pen/strep.

Article Title: Enhanced Bioavailability by Orally Administered Sirolimus Nanocrystals.
Article Snippet: Subscriber access provided by University of Texas Libraries is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

other:

Article Title: Enhanced pathogenicity of Th17 cells due to natalizumab treatment: Implications for MS disease rebound
Article Snippet: Measurement was performed with cellZscope v2.2.2 Software (nanoAnalytics GmbH).



Similar Products

90
nanoAnalytics gmbh cellzscope+ instrument
Cellzscope+ Instrument, supplied by nanoAnalytics gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellzscope/cellzscope+instrument/pmc10103300__nl3c00377_si_001-30-12-14
Average 90 stars, based on 1 article reviews
cellzscope+ instrument - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
nanoAnalytics gmbh cellzscope+ equipment
Cellzscope+ Equipment, supplied by nanoAnalytics gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellzscope/cellzscope++equipment/pmc10723066__jf3c05479_si_001-38-12-14
Average 90 stars, based on 1 article reviews
cellzscope+ equipment - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
nanoAnalytics gmbh cellzscope
Cellzscope, supplied by nanoAnalytics gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellzscope/cellzscope/pm40644968-126-12-13
Average 90 stars, based on 1 article reviews
cellzscope - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
nanoAnalytics gmbh cellzscope -device
Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the <t>cellZscope</t> the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.
Cellzscope Device, supplied by nanoAnalytics gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellzscope/cellzscope+device/pmc12263646-72-8-15
Average 90 stars, based on 1 article reviews
cellzscope -device - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
nanoAnalytics gmbh automated cellzscope equipment
Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the <t>cellZscope</t> the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.
Automated Cellzscope Equipment, supplied by nanoAnalytics gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellzscope/automated+cell+monitoring+system+cellzscope/pmc12186227-343-10-12
Average 90 stars, based on 1 article reviews
automated cellzscope equipment - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the cellZscope the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Mosquito- and tick-borne orthoflaviviruses cross an in vitro endothelial-astrocyte barrier

doi: 10.3389/fcimb.2025.1624636

Figure Lengend Snippet: Transendothelial electrical resistance (TEER) monitored over time after introduction of transwell inserts to the cellZscope the day after seeding of mouse brain microvascular endothelial cells (mBMEC) and four days after astrocyte seeding. Presented are the mean values of n=6 inserts (continuous line) with the standard deviation (SD; dotted lines and filled area) (A) . TEER values obtained at 72h (C) and 120h (D) of incubation in the cellZscope were statistically tested with a repeated measures one-way analysis of variance (ANOVA), followed by Tukey’s test for multiple comparison (C, D) . Barrier permeability assay of inserts after 120h of incubation in the cellZscope . Presented are the values of n=3 inserts with the SD. Horizontal dotted line indicates 1% of FITC-dextran stock solution, whereas horizontal dashed line indicates 0.1% of applied FITC-dextran concentration (B) . FITC-dextran concentrations determined at 240 minutes after apical application were statistically tested with a repeated measures one-way ANOVA, followed by Tukey’s test for multiple comparison (E) . Asterisks indicate statistical significance as follows: p < 0.05*, p < 0.01**, p < 0.0001****.

Article Snippet: A day later inserts were transferred into a cellZscope -device ( cellZscope + ® , nanoAnalytics, Münster, Germany) and cultured until they were confluent.

Techniques: Standard Deviation, Incubation, Comparison, Permeability, Concentration Assay