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ls column  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec ls column
    Ls Column, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 8965 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+sorting+ls+columns/LS+Columns/pm35337337-105-7-9
    Average 99 stars, based on 8965 article reviews
    ls column - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: .. PBMCs were thawed and naïve‐enriched B cells were isolated using the Naïve B Cell Isolation Kit II human (Miltenyi Biotec, #130‐091‐150) with LS columns (Miltenyi Biotec, #130‐042‐401), according to manufacturer's instructions. .. Purified naïve‐enriched B cells were adjusted to 10 6 cells/mL and labelled with 0.25 μM CTV (Invitrogen, # C34557 ) at 37°C for 20 min, according to manufacturer's instructions and cultured in complete (c)RMPI media containing 10% FBS, 2 mM L‐glutamine (Sigma‐Aldrich, #G7513), 1× penicillin–streptomycin (Sigma‐Aldrich, #P0781), and 50 μM 2‐Mercaptoethanol (Thermofisher, #31350‐010).

    Cell Isolation:

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: .. PBMCs were thawed and naïve‐enriched B cells were isolated using the Naïve B Cell Isolation Kit II human (Miltenyi Biotec, #130‐091‐150) with LS columns (Miltenyi Biotec, #130‐042‐401), according to manufacturer's instructions. .. Purified naïve‐enriched B cells were adjusted to 10 6 cells/mL and labelled with 0.25 μM CTV (Invitrogen, # C34557 ) at 37°C for 20 min, according to manufacturer's instructions and cultured in complete (c)RMPI media containing 10% FBS, 2 mM L‐glutamine (Sigma‐Aldrich, #G7513), 1× penicillin–streptomycin (Sigma‐Aldrich, #P0781), and 50 μM 2‐Mercaptoethanol (Thermofisher, #31350‐010).

    Labeling:

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: The cell pellet was then resuspended in 90 μl of 0.5% BSA in DPBS and incubated with 10 μl of anti-CD11b magnetic beads (Miltenyi, Cat#: 130-097-142) for 30 min at 4°C. .. The labeled cells were loaded onto an LS Column (Miltenyi, Cat#: 130-042-401) pre-equilibrated with 10 ml of 0.5% BSA in DPBS and washed with an additional 10 ml of the same buffer. ..

    Magnetic Cell Separation:

    Article Title: TCRαβ repertoire plasticity defines universal CD8+ T cell responses directed at influenza-specific HLA-A*02:01-PB1413-421 epitope.
    Article Snippet: Tetramer-associated magnetic enrichment of epitope-specific CD8+ T cells Tetramer-associated magnetic enrichment (TAME) was performed as described.32 Briefly, 1 to 50 × 106 cryopreserved peripheral blood mononuclear cells (PBMCs) were incubated with anti-human FcR block (Miltenyi Biotec) for 20 min on ice before staining with pMHC-I tetramers conjugated to PE-SA or APC-SA for 1 h at room temperature. .. Cells were washed in MACS buffer, incubated with anti-PE or anti-APC MicroBeads (Miltenyi Biotec) before passing through a LS column (Miltenyi Biotec). ..

    Incubation:

    Article Title: TCRαβ repertoire plasticity defines universal CD8+ T cell responses directed at influenza-specific HLA-A*02:01-PB1413-421 epitope.
    Article Snippet: Tetramer-associated magnetic enrichment of epitope-specific CD8+ T cells Tetramer-associated magnetic enrichment (TAME) was performed as described.32 Briefly, 1 to 50 × 106 cryopreserved peripheral blood mononuclear cells (PBMCs) were incubated with anti-human FcR block (Miltenyi Biotec) for 20 min on ice before staining with pMHC-I tetramers conjugated to PE-SA or APC-SA for 1 h at room temperature. .. Cells were washed in MACS buffer, incubated with anti-PE or anti-APC MicroBeads (Miltenyi Biotec) before passing through a LS column (Miltenyi Biotec). ..



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