incucyte s3 (Sartorius AG)
Sartorius AG is a verified supplier 
Sartorius AG manufactures this product 
99
Structured Review
Sartorius AG
incucyte s3
Incucyte S3, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 16124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+imaging/Live+Cell+Analysis+Instruments/pmc13080321-75-6-8
Average 99 stars, based on 16124 article reviews
Incucyte S3, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 16124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+imaging/Live+Cell+Analysis+Instruments/pmc13080321-75-6-8
Average 99 stars, based on 16124 article reviews
incucyte s3 - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "Base Editing of TIGIT Reprograms CD155 Signaling in Natural Killer Cells to Enhance Cancer Immunotherapy Efficacy"
Article Title: Base Editing of TIGIT Reprograms CD155 Signaling in Natural Killer Cells to Enhance Cancer Immunotherapy Efficacy
Journal: Cancer Research
doi: 10.1158/0008-5472.CAN-25-0733
Figure Legend Snippet: Cytotoxicity activities of TIGIT BE-NK cells in vitro . A–E, A variety of different cancer cell lines (GFP expressing or red dye labeled) were inoculated into 96-well plates in quantities of 10 4 cells/well and left for 6 hours to allow cells to attach, then 10 4 PB-NK or BE-NK cells were added, and images of the same field of view were recorded at different times. A, NSCLC cell line H1299 (GFP expressing). B , NSCLC cell line A549 (GFP expressing). C , Fibrosarcoma cell line HT-1080 (GFP expressing). D , Hepatocellular cell line Huh7 (red dye labeled). E , Hepatoma cell line PLC/PRF/5 (red dye labeled). Images recorded and data analyzed by IncuCyte S3, and data are shown as mean ± SD.
Techniques Used: In Vitro, Expressing, Labeling
Figure Legend Snippet: TIGIT BE-NK cells specifically recognized target cells in a CD155-/CD226-dependent manner. A, The mean fluorescence intensity (MFI) of CD155 on H1299, H1975, A549, and H460 (NSCLC cell line), K652 (chronic myelogenous leukemia cell line), and CEM (acute lymphoblastic leukemia cell line) was assessed by flow cytometry. B, Tumor cells and PB-NK or TIGIT BE-NK cells were inoculated into 48-well plates at a 1:1 ratio, and CD107a expression on NK cells was detected by flow cytometry 4 hours later. The increase in CD107a-positive cells in the TIGIT BE-NK group was normalized to the PB-NK group. C, Tumor cells were coincubated with PB-NK or TIGIT BE-NK cells for 24 hours at an E:T ratio of 1:1, and then NK cell–mediated lysis was evaluated by luminescent cell viability assay. E:T corresponds to E:T cells. D, The mean fluorescence intensity of CD155 on U251-MG (glioblastoma cell line), Huh7 (hepatocellular cell line), HT-1080 (fibrosarcoma cell line), PLC/PRF/5 (hepatoma cell line), BxPC-3 (pancreatic adenocarcinoma cell line), T24 (urinary bladder cancer cell line), U2OS (osteosarcoma cell line), MCF7 (breast cancer cell line), HeLa (cervical carcinoma cell line), SKOV3 (ovarian cancer cell line), HCT116 (colorectal carcinoma cell line), HGC-27 (gastric cancer cell line), KG-1A, OCI-AML3 (acute myeloid leukemia cell line), and MM.1S (multiple myeloma) was assessed by flow cytometry. E, Tumor cells and PB-NK or TIGIT BE-NK cells were inoculated into 48-well plates at a 1:1 ratio, and CD107a expression on NK cells was detected by flow cytometry 4 hours later. The increase in CD107a-positive cells in the TIGIT BE-NK group was normalized to the PB-NK group. F, The correlation analysis of the result is shown in D and E . Simple linear regression analysis. G and H, The NK cell–mediated cytotoxicity was evaluated using a luminescent cell viability assay following coincubation of A549 ( G ) or Huh7 ( H ) cells with NK cells at a 1:1 ratio for 24 hours. I and J, Cytotoxic effects of individual and combined TIGIT, CD96, and PVRIG KO in NK cells. Data recorded and analyzed by IncuCyte S3; data are shown as mean ± SD. K and L, PB-NK, TIGIT BE-NK, or TIGIT/CD226 double–KO NK cells were cocultured with A549 or Huh7 tumor cells. NK cells were harvested at 0, 2, 15, and 30 minutes after stimulation, and p44/42 MAPK (ERK1/2) and phospho-p44/42 MAPK (ERK1/2; Thr202/Tyr204) activity was assessed by Western blotting. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, nonsignificant.
Techniques Used: Fluorescence, Flow Cytometry, Expressing, Lysis, Cell Viability Assay, Activity Assay, Western Blot
Related Articles
Cell Characterization:Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the Proliferation Assay:Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the Software:Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research Article Snippet: .. Article Title: Targeting CD28 on T lineage malignancies with chimeric antigen receptor T cells. Article Snippet: .. Quantification of cellular abundance was performed as recommended by measuring GFP integrated intensity in at least Article Title: Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons. Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss Zen software (Carl Zeiss), Seahorse Wave Desktop Software v2.6 (Agilent Technologies), Harmony software v4.9 (PerkinElmer), Analysis:Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research Article Snippet: .. Article Title: Differential effects of propranolol enantiomers on haemangioma-derived endothelial spheroids Article Snippet: The spheroids were embedded in Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, NE Minneapolis, MN, USA) at a concentration of 4–5 mg/mL, following removal of growth medium and incubated at 37 °C to allow polymerisation. .. Culture media was added on top and whole spheroid area and the sprouting area was monitored over 3 days with the Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Staining:Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis. Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells. Morphology:Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incubation:Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Phase Contrast:Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the |
