cell count normalization kit (Dojindo Labs)

Dojindo Labs is a verified supplier

Dojindo Labs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Dojindo Labs cell count normalization kit
Cell Count Normalization Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+counting/Cell+Count+Normalization+Kit/pm37408086-139-9-13
Average 93 stars, based on 31 article reviews

cell count normalization kit - by Bioz Stars, 2026-10

93/100 stars

Images

Cell Counting:

Article Title: Leucine-rich repeat kinase 2 promotes disintegration of retinal pigment epithelial cell: Implication in the pathogenesis of dry age-related macular degeneration.
Article Snippet: Page 11 of 34 Subsequently, 20 μg/mL of recombinant human α-synuclein fibrils (SPR-322, 1 StressMarq Biosciences Inc.) were added and incubated for 24, 48, and 72 h. 2 Caspase-3/7 activity was measured using caspase-Glo 3/7 Assay (Promega) in 3 accordance with the manufacturer’s instructions. .. 4 5 Cell counting 6 hTERT-RPE1 cells were seeded into clear-bottom 96-well plates at a density of 7 1.5×103 cells/well and incubated for 24 h. Subsequently, 20 μg/mL of recombinant 8 human α-synuclein fibrils (SPR-322, StressMarq Biosciences Inc., Victoria, Canada) 9 and control (PBS buffer) were added and incubated for 24, 48, and 72 h. Cell nuclei 10 were stained with a nuclear staining reagent (Hoechst 33342) using a Cell Count 11 Normalization Kit (#C544; Dojindo, Kumamoto, Japan). .. Three locations per well were 12 photographed, the number of cells in each photograph was counted using the image 13 analysis software ImageJ (https://imagej.net/software/imagej/, version: 1.53t, date of 14 last access: September 10, 2022), and the average value was calculated.

Incubation:

Recombinant:

SPR Assay:

Control:

Staining:

Cell Characterization:

Article Title: A novel senotherapeutic strategy with azithromycin for preventing endometriosis progression
Article Snippet: SA-β-Gal activity was quantitated with a Cellular Senescence Plate Assay Kit using SPiDER-βGal, a fluorogenic substrate for β-galactosidase (SG05, Dojindo, Kumamoto, Japan), following the manufacturer’s instructions. .. The optimal cell numbers in each well were adjusted using a Cell Count Normalization Kit (C544, Dojindo, Kumamoto, Japan), following the manufacturer’s instructions. .. Cells were lysed with Lysis Buffer for 10 min at room temperature, then incubated with SPiDER-βGal working solution at 37 °C for 30 min. After adding a stop solution, using an excitation wavelength of 535 nm, emission at 580 nm was recorded using a multimode microplate reader (SpectraMax iD5, Molecular Devices, San Jose, CA, USA).

Article Title: Carnosol, a Rosemary Ingredient Discovered in a Screen for Inhibitors of SARM1-NAD + Cleavage Activity, Ameliorates Symptoms of Peripheral Neuropathy.
Article Snippet: Luminescence was observed using a Fluoroskan Ascent FL plate reader (Thermo Fisher Scientific). .. To analyze intracellular NAD+ levels, a Cell Count Normalization Kit (Dojindo, Kumamoto, Japan) was used to normalize cell numbers before the NAD+ assay. .. The fluorescence intensity of cells stained with Hoechst 33342 was detected by using a FlexStation 3 microplate reader (Molecular Devices, Sunnyvale, CA, USA; Ex: 350 nm; Em: 461 nm).

Article Title: Carnosol, a Rosemary Ingredient Discovered in a Screen for Inhibitors of SARM1-NAD + Cleavage Activity, Ameliorates Symptoms of Peripheral Neuropathy
Article Snippet: Luminescence was observed using a Fluoroskan Ascent FL plate reader (Thermo Fisher Scientific). .. To analyze intracellular NAD + levels, a Cell Count Normalization Kit (Dojindo, Kumamoto, Japan) was used to normalize cell numbers before the NAD + assay. .. The fluorescence intensity of cells stained with Hoechst 33342 was detected by using a FlexStation 3 microplate reader (Molecular Devices, Sunnyvale, CA, USA; Ex: 350 nm; Em: 461 nm).

Article Title: A human and mouse subpopulation of senescent β-cells induces pathologic dysfunction through targetable paracrine signaling
Article Snippet: Following Xenium, multiplexed antibody-based immunofluorescence staining and imaging was performed using the Phenocycler-Fusion (Akoya Biosciences) according to a custom protocol ( https://dx.doi.org/10.17504/protocols.io.q26g71rwqgwz/v1 )]. .. To measure senescence-associated β-galactosidase (SA-βGal) activity, we used the Dojindo Cell Count Normalization Kit (C544) combined with the Cellular Senescence Plate Assay Kit – Spider-βGal (SG05) following the manufacturer’s protocol for combined analysis. ..

Article Title: A novel senotherapeutic strategy with azithromycin for preventing endometriosis progression.
Article Snippet: SA-β-Gal activity was quantitated with a Cellular Senescence Plate Assay Kit using SPiDER-βGal, a fluorogenic substrate for β-galactosidase (SG05, Dojindo, Kumamoto, Japan), following the manufacturer’s instructions. .. The optimal cell numbers in each well were adjusted using a Cell Count Normalization Kit (C544, Dojindo, Kumamoto, Japan), following the manufacturer’s instructions. .. Cells were lysed with Lysis Buffer for 10 min at room temperature, then incubated with SPiDER-βGal working solution at 37 °C for 30 min. After adding a stop solution, using an excitation wavelength of 535 nm, emission at 580 nm was recorded using a multimode microplate reader (SpectraMax iD5, Molecular Devices, San Jose, CA, USA).

Review