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biotinylated anti human mgl antibody  (R&D Systems)


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    Structured Review

    R&D Systems biotinylated anti human mgl antibody
    Biotinylated Anti Human Mgl Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd301/Human+CLEC10A%2FCD301+Biotinylated+Antibody/pm41006428-75-14-21
    Average 93 stars, based on 1 article reviews
    biotinylated anti human mgl antibody - by Bioz Stars, 2026-11
    93/100 stars

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    other:

    Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy.
    Article Snippet: The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking.

    Incubation:

    Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy
    Article Snippet: The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca 2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking. .. To detect glycoprotein-MGL complexes, the membranes were incubated for 1 h at RT with biotinylated anti-human MGL antibody (anti-human CLEC10A/CD301; BAF4888, R&D Systems, Minneapolis, MN, USA), diluted 1:2000 in TBST. .. For all lectins (MGL, VVL, and WFL), the complexes were detected with ExtrAvidin-alkaline phosphatase (Sigma-Aldrich, St. Louis, MO, USA), diluted 1:10,000 in TBST, and incubated for 45 min at RT, followed by development with BCIP/NBT substrate (Sigma-Aldrich, St. Louis, MO, USA).

    Article Title: An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides.
    Article Snippet: .. Retention of bound CLEC10A was determined by incubation with biotinylated anti-CLEC10A (goat IgG, R&D Systems) and detection with streptavidin-peroxidase conjugate (blue). ..



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    PYGM promotes colorectal cancer cell proliferation, migration, invasion, and M2 macrophage polarization. (A) Protein expression levels of PYGM were detected by western blotting. (B) EDU assay analysis. (C) Apoptosis rate was detected by flow cytometry. (D–F) Migration and invasion assay analysis. (G) Schematic illustration of the indirect co-culture system between colorectal cancer cells and THP-1-derived macrophages. (H) The expression levels of CD206 and <t>CD301</t> were determined by flow cytometry. (I) Arg1, CD206, CD301 and IL-10 gene expression levels were detected by RT-PCR. (J) The protein levels of Arg1, CD206, CD301 and IL-10 were detected by western blotting. *p< 0.05; **p< 0.01; ***p< 0.001, compared to the corresponding groups.
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    PYGM promotes colorectal cancer cell proliferation, migration, invasion, and M2 macrophage polarization. (A) Protein expression levels of PYGM were detected by western blotting. (B) EDU assay analysis. (C) Apoptosis rate was detected by flow cytometry. (D–F) Migration and invasion assay analysis. (G) Schematic illustration of the indirect co-culture system between colorectal cancer cells and THP-1-derived macrophages. (H) The expression levels of CD206 and <t>CD301</t> were determined by flow cytometry. (I) Arg1, CD206, CD301 and IL-10 gene expression levels were detected by RT-PCR. (J) The protein levels of Arg1, CD206, CD301 and IL-10 were detected by western blotting. *p< 0.05; **p< 0.01; ***p< 0.001, compared to the corresponding groups.
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    Image Search Results


    PYGM promotes colorectal cancer cell proliferation, migration, invasion, and M2 macrophage polarization. (A) Protein expression levels of PYGM were detected by western blotting. (B) EDU assay analysis. (C) Apoptosis rate was detected by flow cytometry. (D–F) Migration and invasion assay analysis. (G) Schematic illustration of the indirect co-culture system between colorectal cancer cells and THP-1-derived macrophages. (H) The expression levels of CD206 and CD301 were determined by flow cytometry. (I) Arg1, CD206, CD301 and IL-10 gene expression levels were detected by RT-PCR. (J) The protein levels of Arg1, CD206, CD301 and IL-10 were detected by western blotting. *p< 0.05; **p< 0.01; ***p< 0.001, compared to the corresponding groups.

    Journal: Frontiers in Immunology

    Article Title: Machine learning identifies PYGM as a macrophage polarization–linked metabolic biomarker in rectal cancer prognosis

    doi: 10.3389/fimmu.2025.1639303

    Figure Lengend Snippet: PYGM promotes colorectal cancer cell proliferation, migration, invasion, and M2 macrophage polarization. (A) Protein expression levels of PYGM were detected by western blotting. (B) EDU assay analysis. (C) Apoptosis rate was detected by flow cytometry. (D–F) Migration and invasion assay analysis. (G) Schematic illustration of the indirect co-culture system between colorectal cancer cells and THP-1-derived macrophages. (H) The expression levels of CD206 and CD301 were determined by flow cytometry. (I) Arg1, CD206, CD301 and IL-10 gene expression levels were detected by RT-PCR. (J) The protein levels of Arg1, CD206, CD301 and IL-10 were detected by western blotting. *p< 0.05; **p< 0.01; ***p< 0.001, compared to the corresponding groups.

    Article Snippet: Western blotting used the following primary antibodies: PYGM (1:1,000; ProteinTech, 19716-1-AP), β-actin (1:4,000; ProteinTech, 66009-1-Ig), Arg1(1:1,000; ProteinTech, 16001-1-AP), CD301(1:1,000; ProteinTech, 13590-1-AP), CD206(1:1,000; ProteinTech, 32647-1-AP), IL-10(1:1,000; ProteinTech, 60269-1-Ig).

    Techniques: Migration, Expressing, Western Blot, EdU Assay, Flow Cytometry, Invasion Assay, Co-Culture Assay, Derivative Assay, Gene Expression, Reverse Transcription Polymerase Chain Reaction