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Promega lambda dna concatemers
Lambda Dna Concatemers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+lambda+concatamers/lambda+dna+concatemers/pm20141949-70-67-71
Average 90 stars, based on 1 article reviews

lambda dna concatemers - by Bioz Stars, 2026-11

90/100 stars

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Blocking Assay:

Article Title: Recurrent Melioidosis in Patients in Northeast Thailand Is Frequently Due to Reinfection Rather than Relapse
Article Snippet: .. PFGE was performed on a CHEF-DRIII system (Bio-Rad Laboratories, Richmond, CA) for 44 h at a temperature of 14°C and at 6 V/cm using the following parameters: initial switch time, final switch time, and run time for block I, 10 to 60 V for 18 h; those for block II, 30 to 40 V for 18 h; and those for block III, 50 to 90 V for 8 h. Bacteriophage lambda concatemers were run as the standard (Promega). .. Gels were stained with ethidium bromide, washed in water, and photographed under UV light by using the Gel Doc 1000 system (Bio-Rad).

Article Title: Recurrent Melioidosis in Patients in Northeast Thailand Is Frequently Due to Reinfection Rather than Relapse
Article Snippet: .. 6032 on June 18, 2015 by N O R T H E R N A R IZ O N A U N IV http://jcm .asm .org/ D ow nloaded from 60 V for 18 h; those for block II, 30 to 40 V for 18 h; and those for block III, 50 to 90 V for 8 h. Bacteriophage lambda concatemers were run as the standard (Promega). .. Gels were stained with ethidium bromide, washed in water, and photographed under UV light by using the Gel Doc 1000 system (Bio-Rad).

Lambda DNA Preparation:

Article Title: Necrotizing fasciitis in captive juvenileCrocodylus porosuscaused byStreptococcus agalactiae: an outbreak and review of the animal and human literature
Article Snippet: Slices of DNA-containing agarose plugs were digested overnight with 20 U SmaI (New England BioLabs, Genesearch, Arundel, Queensland, Australia) at 25 xC, then electrophoresed on a CHEF DRIII system (Bio-Rad Laboratories, Hercules, CA, USA) for 25 h at 6 V/cm, with ramped pulse times of 5–40 s in a 1.2% SeaKem Gold agarose gel (BioWhittaker Molecular Applications, Rockland, ME, USA) and 0.5r TBE electrophoresis buffer containing 100 mM thiourea. .. Lambda DNA concatemer PFGE markers (Promega, Madison, WI, USA) were used as DNA size standards. .. The gel was stained with ethidium bromide (0.5 mg/ml, Sigma, Castle Hill, NSW, Australia) for 45 min, destained in 0.5r TBE for 45 min, and photographed under UV illumination.

Article Title: Genomic Diversity in Staphylococcus xylosus
Article Snippet: .. Lambda DNA concatemers were used as molecular size markers (Promega). .. After migration, gels were stained in ethidium bromide, digitized under UV light (Gel Doc 2000; Bio-Rad), and analyzed using the Quantity One quantitation software (Bio-Rad).

Article Title: Biodiversity of indigenous staphylococci of naturally fermented dry sausages and manufacturing environments of small-scale processing units.
Article Snippet: DNA fragments were resolved on a 1% gel with a contour-clamped homogeneous electric field apparatus CHEFDRIII (Bio-Rad, Ivry, France). .. PFGE was performed at 6 V/cm and an including angle of 120 with switch times of 40e100 s for 2 h and 5e35 s for 22 h, or switch times of 100 s for 12 h, 70 s for 16 h and 1e30 s for 12 h depending on the sizes of the DNA fragments to be resolved with 0.5 Tris base-borate-EDTA running buffer maintained at 14 C. Lambda DNA concatemers (Promega) and Staphylococcus cerevisae chromosomal DNA (Bio-Rad) were used as molecular weight markers. .. Gels were stained with ethidium bromide and digitalized with the Gel Doc 2000 apparatus (Bio-Rad).

Molecular Weight:

other:

Article Title: Characterization of Escherichia coli O157:H7 from Downer and Healthy Dairy Cattle in the Upper Midwest Region of the United States
Article Snippet: The resulting DNA fragments were resolved by CHEF-PFGE with a CHEF-DRII apparatus (Bio-Rad Laboratories, Richmond, Calif.) at 200 V for 19 h at 14°C and switch times from 1 to 60 s. Low-range lambda concatemers (Promega) were used as DNA size standards.

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