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Structured Review

IBA GmbH capture buffer bxt
Capture Buffer Bxt, supplied by IBA GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bxt+buffer/capture+buffer+bxt/pmc07804149-173-21-24
Average 90 stars, based on 1 article reviews
capture buffer bxt - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Expressing:

Article Title: Capsid-like particles decorated with the SARS2-CoV-2 receptor-binding domain elicit strong virus neutralization activity
Article Snippet: The crude protein was loaded onto a StreptactinXT (IBA) affinity column. .. 333 Proteins were eluted using capture buffer (100mM Tris-HCl, 150mM NaCl, 1 mM EDTA pH 8.0 ) supplemented 334 with 50mM D-Biotin (BXT buffer, Iba GmbH) 335 336 Design, expression and purification of Tag-CLP 337 The proprietary peptide binding Tag and a linker (GSGTAGGGSGS) was added to the N-terminus of the 338 Acinetobacter phage AP205 coat protein (Gene ID: 956335) by PCR. .. The gene sequence was inserted into the 339 Side 14 af 23 pET28a(+) vector (Novagen) using NcoI (New England Biolabs) and NotI (New England Biolabs) restriction 340 sites.

Purification:

Article Title: Capsid-like particles decorated with the SARS2-CoV-2 receptor-binding domain elicit strong virus neutralization activity
Article Snippet: The crude protein was loaded onto a StreptactinXT (IBA) affinity column. .. 333 Proteins were eluted using capture buffer (100mM Tris-HCl, 150mM NaCl, 1 mM EDTA pH 8.0 ) supplemented 334 with 50mM D-Biotin (BXT buffer, Iba GmbH) 335 336 Design, expression and purification of Tag-CLP 337 The proprietary peptide binding Tag and a linker (GSGTAGGGSGS) was added to the N-terminus of the 338 Acinetobacter phage AP205 coat protein (Gene ID: 956335) by PCR. .. The gene sequence was inserted into the 339 Side 14 af 23 pET28a(+) vector (Novagen) using NcoI (New England Biolabs) and NotI (New England Biolabs) restriction 340 sites.

Binding Assay:

Article Title: Capsid-like particles decorated with the SARS2-CoV-2 receptor-binding domain elicit strong virus neutralization activity
Article Snippet: The crude protein was loaded onto a StreptactinXT (IBA) affinity column. .. 333 Proteins were eluted using capture buffer (100mM Tris-HCl, 150mM NaCl, 1 mM EDTA pH 8.0 ) supplemented 334 with 50mM D-Biotin (BXT buffer, Iba GmbH) 335 336 Design, expression and purification of Tag-CLP 337 The proprietary peptide binding Tag and a linker (GSGTAGGGSGS) was added to the N-terminus of the 338 Acinetobacter phage AP205 coat protein (Gene ID: 956335) by PCR. .. The gene sequence was inserted into the 339 Side 14 af 23 pET28a(+) vector (Novagen) using NcoI (New England Biolabs) and NotI (New England Biolabs) restriction 340 sites.

Polymerase Chain Reaction:

Article Title: Capsid-like particles decorated with the SARS2-CoV-2 receptor-binding domain elicit strong virus neutralization activity
Article Snippet: The crude protein was loaded onto a StreptactinXT (IBA) affinity column. .. 333 Proteins were eluted using capture buffer (100mM Tris-HCl, 150mM NaCl, 1 mM EDTA pH 8.0 ) supplemented 334 with 50mM D-Biotin (BXT buffer, Iba GmbH) 335 336 Design, expression and purification of Tag-CLP 337 The proprietary peptide binding Tag and a linker (GSGTAGGGSGS) was added to the N-terminus of the 338 Acinetobacter phage AP205 coat protein (Gene ID: 956335) by PCR. .. The gene sequence was inserted into the 339 Side 14 af 23 pET28a(+) vector (Novagen) using NcoI (New England Biolabs) and NotI (New England Biolabs) restriction 340 sites.



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