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buffer  (Epizyme Inc)

 
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    Structured Review

    Epizyme Inc buffer
    Buffer, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer/anti+buffer/pm40946305-98-171-175
    Average 86 stars, based on 1 article reviews
    buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Membrane:

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: Next, proteins in the gel were transferred to a 0.2 mm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). .. The membrane was blocked with 3% bovine serum albumin (BSA) for 1 h and incubated with primary antibodies, diluted in primary antibody dilution buffer (Epizyme Biotech, China), against JNK, p-JNK, p38, p-p38, IκBα, pIκBα, p65, p-p65, Tubulin and GAPDH overnight at 4 °C. ..

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance
    Article Snippet: Next, proteins in the gel were transferred to a 0.2 mm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). .. The membrane was blocked with 3% bovine serum albumin (BSA) for 1 h and incubated with primary antibodies, diluted in primary antibody dilution buffer (Epizyme Biotech, China), against JNK, p-JNK, p38, p-p38, IκBα, p-IκBα, p65, p-p65, Tubulin and GAPDH overnight at 4 °C. ..

    Incubation:

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance.
    Article Snippet: Next, proteins in the gel were transferred to a 0.2 mm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). .. The membrane was blocked with 3% bovine serum albumin (BSA) for 1 h and incubated with primary antibodies, diluted in primary antibody dilution buffer (Epizyme Biotech, China), against JNK, p-JNK, p38, p-p38, IκBα, pIκBα, p65, p-p65, Tubulin and GAPDH overnight at 4 °C. ..

    Article Title: Streptococcus thermophilus alleviates fatty liver disease by regulating gut microbiota, lipid metabolism, and inflammation.
    Article Snippet: Alcoholic fatty liver disease (AFLD) is a liver disease caused by long-term excessive alcohol consumption, and there remains no effective treatment beyond alcohol abstinence.. Streptococcus thermophilus (S. thermophilus), a traditional probiotic, is widely used in yogurt and other fermented dairy products.. This study investigated the therapeutic effects of S. thermophilus in AFLD mice.

    Article Title: A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice
    Article Snippet: Tissues were sectioned to a thickness of 40 μm with Thermo HM525-NX and were performed antigen retrieval at 70°C in sodium citrate solution for an hour. .. Antibodies were diluted in primary antibody dilution buffer (Epizyme) and incubated as following ratio: anti-Ube3a (1:500; A300-352A, Bethyl), anti-Flag (1:500; F1084, Sigma-Aldrich), anti-NeuN (1:2,000,MAB377,Chemicon) overnight at 4°C, and then incubated with Alexa Fluor 488 or Alexa Fluor 647 goat secondary antibody to rabbit IgG and Alexa Fluor 568 goat antibody to mouse IgG (1:500, Invitrogen) for 2 h at 4°C. ..

    Article Title: Faecalibacterium prausnitzii alleviates experimental recurrent acute pancreatitis by producing oleic acid to regulate MAPK/NF-κB signaling and Th17/Treg balance
    Article Snippet: Next, proteins in the gel were transferred to a 0.2 mm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). .. The membrane was blocked with 3% bovine serum albumin (BSA) for 1 h and incubated with primary antibodies, diluted in primary antibody dilution buffer (Epizyme Biotech, China), against JNK, p-JNK, p38, p-p38, IκBα, p-IκBα, p65, p-p65, Tubulin and GAPDH overnight at 4 °C. ..

    Blocking Assay:

    Article Title: Streptococcus thermophilus alleviates fatty liver disease by regulating gut microbiota, lipid metabolism, and inflammation.
    Article Snippet: Alcoholic fatty liver disease (AFLD) is a liver disease caused by long-term excessive alcohol consumption, and there remains no effective treatment beyond alcohol abstinence.. Streptococcus thermophilus (S. thermophilus), a traditional probiotic, is widely used in yogurt and other fermented dairy products.. This study investigated the therapeutic effects of S. thermophilus in AFLD mice.

    Binding Assay:

    Article Title: Streptococcus thermophilus alleviates fatty liver disease by regulating gut microbiota, lipid metabolism, and inflammation.
    Article Snippet: Alcoholic fatty liver disease (AFLD) is a liver disease caused by long-term excessive alcohol consumption, and there remains no effective treatment beyond alcohol abstinence.. Streptococcus thermophilus (S. thermophilus), a traditional probiotic, is widely used in yogurt and other fermented dairy products.. This study investigated the therapeutic effects of S. thermophilus in AFLD mice.

    Western Blot:

    Article Title: Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells
    Article Snippet: Primary antibody dilution buffer for western blot , Epizyme , Cat# PS114. .. Secondary antibody dilution buffer for western blot , Epizyme , Cat# PS115. .. SurePAGE , GenScript , Cat# M00654.

    Article Title: Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag-HRP (6E2) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 HA-Tag (C29F4) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Anti-Ubiquitin-HRP (P4D1) (WB: 1/2,000) BioLegend Cat# 646303; RRID:AB_2629597 AF647 anti-human LAG3 (3DS223H) (FC: 1/500) Invitrogen Cat# 51-2239-42; RRID:AB_2744740 Relatlimab Jiang et al. 1 N/A HRP goat anti-rabbit IgG (H+L) (WB: 1/5,000) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Chemicals, peptides, and recombinant proteins Staphylococcal Enterotoxin E (SEE) Toxin Technologies Cat# ET404 Hieff Trans liposomal transfection reagent Yeasen Cat# 40802ES08 N-ethylmaleimide (NEM) Sigma-Aldrich Cat# E3876 Protease inhibitor cocktail Selleck Cat# B14002 EZview Red anti-HA affinity gel Sigma-Aldrich Cat# E6779 63 protein loading buffer TransGen Cat# DL101-02 RPMI 1640 Thermo Fisher Scientific Cat# C22400500CP Fetal bovine serum (FBS) Gibco Cat# 16000-044 DMEM Thermo Fisher Scientific Cat# C11995500BT Triton X-100 Sigma-Aldrich Cat# T8787 20x MOPS SDS running buffer ABCONE Cat# M22785-500ML 10x Tris buffered saline with Tween 20 ABCONE Cat# T71236-1L Transfer buffer (103) Epizyme Cat# PS109 Primary antibody dilution buffer for western blot Epizyme Cat# PS114 Secondary antibody dilution buffer for western blot Epizyme Cat# PS115 SurePAGE GenScript Cat# M00654 PageRuler prestained protein ladder Thermo Scientific Cat# 26617 SuperPico ECL master mix Vazyme Cat# E432-02 (Continued on next page) STAR Protocols 6, 104090, December 19, 2025 5 .. Reagents for cell culture Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell staining buffer 4A Biotech Cat# FXP005 Critical commercial assays Zombie Violet fixable viability kit BioLegend Cat# 423114 EndoFree Mini plasmid kit II TIANGEN Cat# DP118-02 Experimental models: Cell lines HEK-293FT (human fetal epithelial cells) Cell Bank of Chinese Academy of Sciences Cat#GNHu 43 Jurkat E6.1 T cells Gifted from Arthur Weiss Lab N/A Raji Gifted from Arthur Weiss Lab N/A Recombinant DNA pCMVdR8.91 Li et al. 5 N/A pMD2.G Li et al. 5 RRID:Addgene_12259 pHR-PGK Morsut et al. 6 RRID:Addgene_79120 pHR-PGK HA-LAG3 Jiang et al. 1 N/A pHR-PGK HA-LAG3-K498R Jiang et al. 1 N/A Software and algorithms FlowJo v.10 TreeStar https://www.flowjo.com/ ImageJ NIH https://imagej.nih.gov/ij/download.html Other Amersham ImageQuant 800 (AI800) GE Healthcare N/A Vi-CELL BLU Beckman N/A LSRFortessa X-20 BD N/A Syringe driven filters BIOFIL Cat# FPE404030 DMEM basic medium (for culturing 293FT cells) Reagent Final concentration Amount DMEM basic N/A 445 mL FBS 10% 50 mL Penicillin/streptomycin (1003) 1 3 5 mL Total N/A 500 mL Store at 4 ◦ C for up to 3 weeks.

    Article Title: Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells
    Article Snippet: Transfer buffer (10×) , Epizyme , Cat# PS109. .. Primary antibody dilution buffer for western blot , Epizyme , Cat# PS114. .. Secondary antibody dilution buffer for western blot , Epizyme , Cat# PS115.

    Recombinant:

    Article Title: Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag-HRP (6E2) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 HA-Tag (C29F4) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Anti-Ubiquitin-HRP (P4D1) (WB: 1/2,000) BioLegend Cat# 646303; RRID:AB_2629597 AF647 anti-human LAG3 (3DS223H) (FC: 1/500) Invitrogen Cat# 51-2239-42; RRID:AB_2744740 Relatlimab Jiang et al. 1 N/A HRP goat anti-rabbit IgG (H+L) (WB: 1/5,000) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Chemicals, peptides, and recombinant proteins Staphylococcal Enterotoxin E (SEE) Toxin Technologies Cat# ET404 Hieff Trans liposomal transfection reagent Yeasen Cat# 40802ES08 N-ethylmaleimide (NEM) Sigma-Aldrich Cat# E3876 Protease inhibitor cocktail Selleck Cat# B14002 EZview Red anti-HA affinity gel Sigma-Aldrich Cat# E6779 63 protein loading buffer TransGen Cat# DL101-02 RPMI 1640 Thermo Fisher Scientific Cat# C22400500CP Fetal bovine serum (FBS) Gibco Cat# 16000-044 DMEM Thermo Fisher Scientific Cat# C11995500BT Triton X-100 Sigma-Aldrich Cat# T8787 20x MOPS SDS running buffer ABCONE Cat# M22785-500ML 10x Tris buffered saline with Tween 20 ABCONE Cat# T71236-1L Transfer buffer (103) Epizyme Cat# PS109 Primary antibody dilution buffer for western blot Epizyme Cat# PS114 Secondary antibody dilution buffer for western blot Epizyme Cat# PS115 SurePAGE GenScript Cat# M00654 PageRuler prestained protein ladder Thermo Scientific Cat# 26617 SuperPico ECL master mix Vazyme Cat# E432-02 (Continued on next page) STAR Protocols 6, 104090, December 19, 2025 5 .. Reagents for cell culture Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell staining buffer 4A Biotech Cat# FXP005 Critical commercial assays Zombie Violet fixable viability kit BioLegend Cat# 423114 EndoFree Mini plasmid kit II TIANGEN Cat# DP118-02 Experimental models: Cell lines HEK-293FT (human fetal epithelial cells) Cell Bank of Chinese Academy of Sciences Cat#GNHu 43 Jurkat E6.1 T cells Gifted from Arthur Weiss Lab N/A Raji Gifted from Arthur Weiss Lab N/A Recombinant DNA pCMVdR8.91 Li et al. 5 N/A pMD2.G Li et al. 5 RRID:Addgene_12259 pHR-PGK Morsut et al. 6 RRID:Addgene_79120 pHR-PGK HA-LAG3 Jiang et al. 1 N/A pHR-PGK HA-LAG3-K498R Jiang et al. 1 N/A Software and algorithms FlowJo v.10 TreeStar https://www.flowjo.com/ ImageJ NIH https://imagej.nih.gov/ij/download.html Other Amersham ImageQuant 800 (AI800) GE Healthcare N/A Vi-CELL BLU Beckman N/A LSRFortessa X-20 BD N/A Syringe driven filters BIOFIL Cat# FPE404030 DMEM basic medium (for culturing 293FT cells) Reagent Final concentration Amount DMEM basic N/A 445 mL FBS 10% 50 mL Penicillin/streptomycin (1003) 1 3 5 mL Total N/A 500 mL Store at 4 ◦ C for up to 3 weeks.

    Transfection:

    Article Title: Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag-HRP (6E2) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 HA-Tag (C29F4) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Anti-Ubiquitin-HRP (P4D1) (WB: 1/2,000) BioLegend Cat# 646303; RRID:AB_2629597 AF647 anti-human LAG3 (3DS223H) (FC: 1/500) Invitrogen Cat# 51-2239-42; RRID:AB_2744740 Relatlimab Jiang et al. 1 N/A HRP goat anti-rabbit IgG (H+L) (WB: 1/5,000) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Chemicals, peptides, and recombinant proteins Staphylococcal Enterotoxin E (SEE) Toxin Technologies Cat# ET404 Hieff Trans liposomal transfection reagent Yeasen Cat# 40802ES08 N-ethylmaleimide (NEM) Sigma-Aldrich Cat# E3876 Protease inhibitor cocktail Selleck Cat# B14002 EZview Red anti-HA affinity gel Sigma-Aldrich Cat# E6779 63 protein loading buffer TransGen Cat# DL101-02 RPMI 1640 Thermo Fisher Scientific Cat# C22400500CP Fetal bovine serum (FBS) Gibco Cat# 16000-044 DMEM Thermo Fisher Scientific Cat# C11995500BT Triton X-100 Sigma-Aldrich Cat# T8787 20x MOPS SDS running buffer ABCONE Cat# M22785-500ML 10x Tris buffered saline with Tween 20 ABCONE Cat# T71236-1L Transfer buffer (103) Epizyme Cat# PS109 Primary antibody dilution buffer for western blot Epizyme Cat# PS114 Secondary antibody dilution buffer for western blot Epizyme Cat# PS115 SurePAGE GenScript Cat# M00654 PageRuler prestained protein ladder Thermo Scientific Cat# 26617 SuperPico ECL master mix Vazyme Cat# E432-02 (Continued on next page) STAR Protocols 6, 104090, December 19, 2025 5 .. Reagents for cell culture Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell staining buffer 4A Biotech Cat# FXP005 Critical commercial assays Zombie Violet fixable viability kit BioLegend Cat# 423114 EndoFree Mini plasmid kit II TIANGEN Cat# DP118-02 Experimental models: Cell lines HEK-293FT (human fetal epithelial cells) Cell Bank of Chinese Academy of Sciences Cat#GNHu 43 Jurkat E6.1 T cells Gifted from Arthur Weiss Lab N/A Raji Gifted from Arthur Weiss Lab N/A Recombinant DNA pCMVdR8.91 Li et al. 5 N/A pMD2.G Li et al. 5 RRID:Addgene_12259 pHR-PGK Morsut et al. 6 RRID:Addgene_79120 pHR-PGK HA-LAG3 Jiang et al. 1 N/A pHR-PGK HA-LAG3-K498R Jiang et al. 1 N/A Software and algorithms FlowJo v.10 TreeStar https://www.flowjo.com/ ImageJ NIH https://imagej.nih.gov/ij/download.html Other Amersham ImageQuant 800 (AI800) GE Healthcare N/A Vi-CELL BLU Beckman N/A LSRFortessa X-20 BD N/A Syringe driven filters BIOFIL Cat# FPE404030 DMEM basic medium (for culturing 293FT cells) Reagent Final concentration Amount DMEM basic N/A 445 mL FBS 10% 50 mL Penicillin/streptomycin (1003) 1 3 5 mL Total N/A 500 mL Store at 4 ◦ C for up to 3 weeks.

    Protease Inhibitor:

    Article Title: Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag-HRP (6E2) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 HA-Tag (C29F4) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Anti-Ubiquitin-HRP (P4D1) (WB: 1/2,000) BioLegend Cat# 646303; RRID:AB_2629597 AF647 anti-human LAG3 (3DS223H) (FC: 1/500) Invitrogen Cat# 51-2239-42; RRID:AB_2744740 Relatlimab Jiang et al. 1 N/A HRP goat anti-rabbit IgG (H+L) (WB: 1/5,000) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Chemicals, peptides, and recombinant proteins Staphylococcal Enterotoxin E (SEE) Toxin Technologies Cat# ET404 Hieff Trans liposomal transfection reagent Yeasen Cat# 40802ES08 N-ethylmaleimide (NEM) Sigma-Aldrich Cat# E3876 Protease inhibitor cocktail Selleck Cat# B14002 EZview Red anti-HA affinity gel Sigma-Aldrich Cat# E6779 63 protein loading buffer TransGen Cat# DL101-02 RPMI 1640 Thermo Fisher Scientific Cat# C22400500CP Fetal bovine serum (FBS) Gibco Cat# 16000-044 DMEM Thermo Fisher Scientific Cat# C11995500BT Triton X-100 Sigma-Aldrich Cat# T8787 20x MOPS SDS running buffer ABCONE Cat# M22785-500ML 10x Tris buffered saline with Tween 20 ABCONE Cat# T71236-1L Transfer buffer (103) Epizyme Cat# PS109 Primary antibody dilution buffer for western blot Epizyme Cat# PS114 Secondary antibody dilution buffer for western blot Epizyme Cat# PS115 SurePAGE GenScript Cat# M00654 PageRuler prestained protein ladder Thermo Scientific Cat# 26617 SuperPico ECL master mix Vazyme Cat# E432-02 (Continued on next page) STAR Protocols 6, 104090, December 19, 2025 5 .. Reagents for cell culture Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell staining buffer 4A Biotech Cat# FXP005 Critical commercial assays Zombie Violet fixable viability kit BioLegend Cat# 423114 EndoFree Mini plasmid kit II TIANGEN Cat# DP118-02 Experimental models: Cell lines HEK-293FT (human fetal epithelial cells) Cell Bank of Chinese Academy of Sciences Cat#GNHu 43 Jurkat E6.1 T cells Gifted from Arthur Weiss Lab N/A Raji Gifted from Arthur Weiss Lab N/A Recombinant DNA pCMVdR8.91 Li et al. 5 N/A pMD2.G Li et al. 5 RRID:Addgene_12259 pHR-PGK Morsut et al. 6 RRID:Addgene_79120 pHR-PGK HA-LAG3 Jiang et al. 1 N/A pHR-PGK HA-LAG3-K498R Jiang et al. 1 N/A Software and algorithms FlowJo v.10 TreeStar https://www.flowjo.com/ ImageJ NIH https://imagej.nih.gov/ij/download.html Other Amersham ImageQuant 800 (AI800) GE Healthcare N/A Vi-CELL BLU Beckman N/A LSRFortessa X-20 BD N/A Syringe driven filters BIOFIL Cat# FPE404030 DMEM basic medium (for culturing 293FT cells) Reagent Final concentration Amount DMEM basic N/A 445 mL FBS 10% 50 mL Penicillin/streptomycin (1003) 1 3 5 mL Total N/A 500 mL Store at 4 ◦ C for up to 3 weeks.

    Saline:

    Article Title: Protocol for detecting ligand-induced ubiquitination of LAG3 in human T cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag-HRP (6E2) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 HA-Tag (C29F4) (WB: 1/2,000) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Anti-Ubiquitin-HRP (P4D1) (WB: 1/2,000) BioLegend Cat# 646303; RRID:AB_2629597 AF647 anti-human LAG3 (3DS223H) (FC: 1/500) Invitrogen Cat# 51-2239-42; RRID:AB_2744740 Relatlimab Jiang et al. 1 N/A HRP goat anti-rabbit IgG (H+L) (WB: 1/5,000) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Chemicals, peptides, and recombinant proteins Staphylococcal Enterotoxin E (SEE) Toxin Technologies Cat# ET404 Hieff Trans liposomal transfection reagent Yeasen Cat# 40802ES08 N-ethylmaleimide (NEM) Sigma-Aldrich Cat# E3876 Protease inhibitor cocktail Selleck Cat# B14002 EZview Red anti-HA affinity gel Sigma-Aldrich Cat# E6779 63 protein loading buffer TransGen Cat# DL101-02 RPMI 1640 Thermo Fisher Scientific Cat# C22400500CP Fetal bovine serum (FBS) Gibco Cat# 16000-044 DMEM Thermo Fisher Scientific Cat# C11995500BT Triton X-100 Sigma-Aldrich Cat# T8787 20x MOPS SDS running buffer ABCONE Cat# M22785-500ML 10x Tris buffered saline with Tween 20 ABCONE Cat# T71236-1L Transfer buffer (103) Epizyme Cat# PS109 Primary antibody dilution buffer for western blot Epizyme Cat# PS114 Secondary antibody dilution buffer for western blot Epizyme Cat# PS115 SurePAGE GenScript Cat# M00654 PageRuler prestained protein ladder Thermo Scientific Cat# 26617 SuperPico ECL master mix Vazyme Cat# E432-02 (Continued on next page) STAR Protocols 6, 104090, December 19, 2025 5 .. Reagents for cell culture Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell staining buffer 4A Biotech Cat# FXP005 Critical commercial assays Zombie Violet fixable viability kit BioLegend Cat# 423114 EndoFree Mini plasmid kit II TIANGEN Cat# DP118-02 Experimental models: Cell lines HEK-293FT (human fetal epithelial cells) Cell Bank of Chinese Academy of Sciences Cat#GNHu 43 Jurkat E6.1 T cells Gifted from Arthur Weiss Lab N/A Raji Gifted from Arthur Weiss Lab N/A Recombinant DNA pCMVdR8.91 Li et al. 5 N/A pMD2.G Li et al. 5 RRID:Addgene_12259 pHR-PGK Morsut et al. 6 RRID:Addgene_79120 pHR-PGK HA-LAG3 Jiang et al. 1 N/A pHR-PGK HA-LAG3-K498R Jiang et al. 1 N/A Software and algorithms FlowJo v.10 TreeStar https://www.flowjo.com/ ImageJ NIH https://imagej.nih.gov/ij/download.html Other Amersham ImageQuant 800 (AI800) GE Healthcare N/A Vi-CELL BLU Beckman N/A LSRFortessa X-20 BD N/A Syringe driven filters BIOFIL Cat# FPE404030 DMEM basic medium (for culturing 293FT cells) Reagent Final concentration Amount DMEM basic N/A 445 mL FBS 10% 50 mL Penicillin/streptomycin (1003) 1 3 5 mL Total N/A 500 mL Store at 4 ◦ C for up to 3 weeks.



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