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Structured Review

NZYTech Inc bsa blocking solution
Bsa Blocking Solution, supplied by NZYTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsa+blocking+solution/bsa+blocking+solution/pm40347673-96-35-38
Average 90 stars, based on 1 article reviews
bsa blocking solution - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Lead as an environmental toxicant in models of synucleinopathies.
Article Snippet: At the end of the lead exposure protocol, primary whole brain neuronal cultures and H4 cells were washed with 1x DPBS (PAN Biotech, Aidenbach, Germany) and fixed with 4 % of paraformaldehyde solution (PFA) for 20 min (home-made) at room temperature (RT). .. Cells were then washed 3 times with DPBS 1x solution (PAN Biotech, Aidenbach, Germany) and permeabilized with 0.1 % Triton X-100 (Sigma-Aldrich, MO, USA) for 10 min at RT, and then incubated with 3 % BSA in PBS (NZYTech) blocking solution for 1h at RT. .. Incubation with primary antibodies (1:1000) was performed overnight at 4 ◦C [primary neuronal cultures: MAP2 (mouse, ab11267, abcam), phosphorylated alpha synuclein S129 – paSyn S129 (rabbit, ab51253, abcam) and H4 cells: alpha synuclein (mouse, 610787, BD Biosciences)].

Blocking Assay:

Article Title: Lead as an environmental toxicant in models of synucleinopathies.
Article Snippet: At the end of the lead exposure protocol, primary whole brain neuronal cultures and H4 cells were washed with 1x DPBS (PAN Biotech, Aidenbach, Germany) and fixed with 4 % of paraformaldehyde solution (PFA) for 20 min (home-made) at room temperature (RT). .. Cells were then washed 3 times with DPBS 1x solution (PAN Biotech, Aidenbach, Germany) and permeabilized with 0.1 % Triton X-100 (Sigma-Aldrich, MO, USA) for 10 min at RT, and then incubated with 3 % BSA in PBS (NZYTech) blocking solution for 1h at RT. .. Incubation with primary antibodies (1:1000) was performed overnight at 4 ◦C [primary neuronal cultures: MAP2 (mouse, ab11267, abcam), phosphorylated alpha synuclein S129 – paSyn S129 (rabbit, ab51253, abcam) and H4 cells: alpha synuclein (mouse, 610787, BD Biosciences)].



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