adult normal human brain tissue lysates (Novus Biologicals)

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Novus Biologicals adult normal human brain tissue lysates

a Comparison of CHSY1 gene expression in glioma subtypes and <t>normal</t> <t>brain</t> <t>tissue</t> in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal <t>human</t> brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.

Adult Normal Human Brain Tissue Lysates, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brain+tissue/Human+Brain+Tissue+MicroArray+(Normal)/pmc07000683-223-0-9
Average 93 stars, based on 2 article reviews

adult normal human brain tissue lysates - by Bioz Stars, 2026-10

93/100 stars

Images

1) Product Images from "Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability"

Article Title: Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability

Journal: Oncogenesis

doi: 10.1038/s41389-020-0197-0

a Comparison of CHSY1 gene expression in glioma subtypes and normal brain tissue in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal human brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.

Figure Legend Snippet: a Comparison of CHSY1 gene expression in glioma subtypes and normal brain tissue in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal human brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.

Techniques Used: Comparison, Gene Expression, Microarray, Expressing, Immunohistochemistry, Staining, Amplification, MANN-WHITNEY, Western Blot

In Situ Hybridization:

Article Title: FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system
Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296). .. Chromogenic double in situ hybridization was performed for the human brain tissue using the RNAscope 2.5 HD Duplex Assay (Advanced Cell Diagnostics) according to the manufacturer's protocol. .. A Fluorescent Multiplex assay was used for the human retina tissue according to the manufacturer's protocol (Advanced Cell Diagnostics).

RNAscope:

Article Title: Transcriptomic analysis of the human habenula in schizophrenia
Article Snippet: .. smFISH experiments were performed on postmortem human brain tissue using RNAScope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) and 4-Plex Ancillary Kit as previously described . ..

Labeling:

Article Title: Isoglutaminyl cyclase contributes to CCL2-driven neuroinflammation in Alzheimer’s disease
Article Snippet: .. In human brain tissue, the goat antiserum sc-1784 and the mouse monoclonal antibody MAB2791 (R&D Systems) demonstrated distinct neuronal CCL2 labeling comparable to the staining pattern observed in mouse brain. ..

Staining:

Multiplex Assay:

other:

Article Title: FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system
Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296).

Review