Review



boltzmann sigmoidal function  (OriginLab corp)


Bioz Manufacturer Symbol OriginLab corp manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    OriginLab corp boltzmann sigmoidal function
    Boltzmann Sigmoidal Function, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+function/boltzmann+sigmoidal+function/pm39798757-74-10-18
    Average 90 stars, based on 1 article reviews
    boltzmann sigmoidal function - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Purification:

    Article Title: Engineering of a hydroxysteroid dehydrogenase with simultaneous enhancement in activity and thermostability for efficient biosynthesis of ursodeoxycholic acid.
    Article Snippet: Hydroxysteroid dehydrogenases (HSDHs) catalyze the oxidation/reduction of hydroxyl/keto groups of steroids with high regioor stereoselectivity, playing an essential role in producing optically pure chemicals.. In this work, a novel approach was developed to simultaneously improve the stability and activity of 7β-hydroxyste roid dehydrogenase (7β-HSDH) by combining B-factor analysis and computer-aided prediction.. Several advantageous mutants were identified, and the most promising variant, S51Y/P202Y, exhibited 2.3-fold improvements in catalytic activity, 3.3-fold in half-life at 40°C, and 4.7-fold in catalytic efficiency (kcat/Km), respectively.

    Incubation:

    Article Title: Engineering of a hydroxysteroid dehydrogenase with simultaneous enhancement in activity and thermostability for efficient biosynthesis of ursodeoxycholic acid.
    Article Snippet: Hydroxysteroid dehydrogenases (HSDHs) catalyze the oxidation/reduction of hydroxyl/keto groups of steroids with high regioor stereoselectivity, playing an essential role in producing optically pure chemicals.. In this work, a novel approach was developed to simultaneously improve the stability and activity of 7β-hydroxyste roid dehydrogenase (7β-HSDH) by combining B-factor analysis and computer-aided prediction.. Several advantageous mutants were identified, and the most promising variant, S51Y/P202Y, exhibited 2.3-fold improvements in catalytic activity, 3.3-fold in half-life at 40°C, and 4.7-fold in catalytic efficiency (kcat/Km), respectively.

    Activity Assay:

    Article Title: Engineering of a hydroxysteroid dehydrogenase with simultaneous enhancement in activity and thermostability for efficient biosynthesis of ursodeoxycholic acid.
    Article Snippet: Hydroxysteroid dehydrogenases (HSDHs) catalyze the oxidation/reduction of hydroxyl/keto groups of steroids with high regioor stereoselectivity, playing an essential role in producing optically pure chemicals.. In this work, a novel approach was developed to simultaneously improve the stability and activity of 7β-hydroxyste roid dehydrogenase (7β-HSDH) by combining B-factor analysis and computer-aided prediction.. Several advantageous mutants were identified, and the most promising variant, S51Y/P202Y, exhibited 2.3-fold improvements in catalytic activity, 3.3-fold in half-life at 40°C, and 4.7-fold in catalytic efficiency (kcat/Km), respectively.

    Inhibition:

    Article Title: Preclinical Studies and Drug Combination of Low-Cost Molecules for Chagas Disease
    Article Snippet: .. This is determined by plotting the % inhibition against the log 10 of the concentration and fitting the points to a sigmoid Boltzmann curve (dose–response curve) using OriginLab 8.5. ..

    Concentration Assay:

    Article Title: Preclinical Studies and Drug Combination of Low-Cost Molecules for Chagas Disease
    Article Snippet: .. This is determined by plotting the % inhibition against the log 10 of the concentration and fitting the points to a sigmoid Boltzmann curve (dose–response curve) using OriginLab 8.5. ..

    Protein Concentration:

    Article Title: The feruloyl esterase from Thermobacillus xylanilyticus shows broad specificity for processing pre-biotic feruloylated xylooligosaccharides at high temperatures.
    Article Snippet: Ferulic acid has antioxidant properties of interest to the food industry and can be released from natural plant fibres using feruloyl esterases.. Esterases active at high temperatures are highly desirable but currently underrepresented.. Here we report the biochemical characterization of the feruloyl esterase from Thermobacillus xylanilyticus.

    Software:

    Article Title: Physico-chemical characterization of bilirubin-10-sulfonate and comparison of its acid–base behavior with unconjugated bilirubin
    Article Snippet: .. The position of the inflection points was determined using the numerical interpolation procedure using the standard Boltzmann sigmoidal function defined in the Origin software (Origin (Pro), Version 2016, OriginLab Corporation, Northampton, MA, USA Corporation, MA USA). ..

    other:

    Article Title: I-motif formation in the promoter region of the B-MYB proto-oncogene.
    Article Snippet: The data were normalized from 0 to 1 and the Boltzmann sigmoidal function was employed using OriginPro 2021 (OriginLab Corporation, Northampton, Massachusetts, USA).



    Similar Products

    90
    GraphPad Software Inc boltzmann sigmoid function in graphpad prism 10
    Boltzmann Sigmoid Function In Graphpad Prism 10, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+function/boltzman+sigmoidal+fit+function+in+graphpad+prism+6+software/pm40327692-251-10-10
    Average 90 stars, based on 1 article reviews
    boltzmann sigmoid function in graphpad prism 10 - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    GraphPad Software Inc boltzmann sigmoidal function
    Boltzmann Sigmoidal Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+function/boltzmann+sigmoidal+equation/pm39856061-242-10-13
    Average 90 stars, based on 1 article reviews
    boltzmann sigmoidal function - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    OriginLab corp boltzmann sigmoidal function
    Boltzmann Sigmoidal Function, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+function/boltzmann+sigmoidal+function/pm39798757-74-10-18
    Average 90 stars, based on 1 article reviews
    boltzmann sigmoidal function - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    GraphPad Software Inc boltzmann sigmoid function
    Boltzmann Sigmoid Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+function/boltzmann+sigmoidal+equation/pm39394803-136-6-10
    Average 90 stars, based on 1 article reviews
    boltzmann sigmoid function - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    GraphPad Software Inc boltzmann sigmoidal function in graphpad prism
    Boltzmann Sigmoidal Function In Graphpad Prism, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+function/boltzman+sigmoidal+fit+function+in+graphpad+prism+6+software/pm38683783-94-22-22
    Average 90 stars, based on 1 article reviews
    boltzmann sigmoidal function in graphpad prism - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    GraphPad Software Inc nonlinear regression boltzmann sigmoid function
    ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a <t>Boltzmann</t> sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.
    Nonlinear Regression Boltzmann Sigmoid Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/boltzmann+sigmoidal+function/boltzman+sigmoidal+fit+function+in+graphpad+prism+6+software/pmc11029814-336-16-20
    Average 90 stars, based on 1 article reviews
    nonlinear regression boltzmann sigmoid function - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a Boltzmann sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.

    Journal: Science Advances

    Article Title: The immune-evasive proline-283 substitution in influenza nucleoprotein increases aggregation propensity without altering the native structure

    doi: 10.1126/sciadv.adl6144

    Figure Lengend Snippet: ( A ) Representative SYPRO Orange thermal unfolding curve of Pro 283 NP, Ser 283 NP, and SYPRO Orange dye with buffer only. Means of four technical replicates are shown with the shaded area representing the SD. ( B ) Circular dichroism spectra of Pro 283 and Ser 283 NP. ( C ) Irreversible T agg was calculated by fitting the plots from (A) to a Boltzmann sigmoidal curve. The experiment was performed at three different NP concentrations. Individual measurements for five technical replicates are shown (**** P < 0.0001). ( D ) Schematic for a cellular thermal shift assay experiment. Intact cells ectopically expressing NP are harvested, split into aliquots, and heated rapidly to the indicated temperatures in a thermocycler. After centrifugation to pellet protein aggregates, soluble NP remaining in the supernatant is quantified via immunoblot. ( E ) Quantification of cellular thermal shift assay immunoblots. The mean of biological triplicates is shown with the error bars representing the SD (* P < 0.05). All band intensities were normalized to 37°C. Original blots are shown in fig. S2.

    Article Snippet: For differential scanning fluorimetry, T agg values were obtained by performing a nonlinear regression to a Boltzmann sigmoid function using GraphPad Prism.

    Techniques: Circular Dichroism, Thermal Shift Assay, Expressing, Centrifugation, Western Blot