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Structured Review

Becton Dickinson bioscience fixation buffer
Bioscience Fixation Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioscience+fixation/bioscience+fixation+buffer/pmc07272736-259-9-7
Average 90 stars, based on 1 article reviews
bioscience fixation buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: Depletion of microRNA-451 in response to allergen exposure accentuates asthmatic inflammation by regulating Sirtuin2
Article Snippet: Cells collected from BAL fluid were incubated with Fc-blocking anti-mouse CD16/32 antibody (no. 553142, BD Bioscience PharMingen) followed by PE-conjugated anti-SiglecF, PE-Cy7-conjugated CD11c, and APC-conjugated anti-CD11b antibodies. .. For intracellular staining, cells were fixed with BD Bioscience Fixation Buffer for 10 min at room temperature. ..

Incubation:

Article Title: The CD33xCD123xCD70 Multispecific CD3-Engaging DARPin MP0533 Induces Selective T Cell–Mediated Killing of AML Leukemic Stem Cells
Article Snippet: After again two washing steps, 100 μL/well goat anti-rabbit IgG (for detection of DARPins; ThermoFisher Scientific), goat-anti human IgG (for detection of CD123-CD3 ADAPTIR; ThermoFisher Scientific), or mouse anti-Penta His (for detection of CD33-CD3 BiTE and CD123-CD3 DART; Qiagen) antibodies (1:1,000 diluted in FACS buffer) were added to wells. .. After subsequent incubation at 4°C for 20 minutes, cells were again washed twice with FACS buffer and finally fixed with BD Biosciences Fixation Buffer (1:10 diluted in water). ..

FACS:

Article Title: The CD33xCD123xCD70 Multispecific CD3-Engaging DARPin MP0533 Induces Selective T Cell–Mediated Killing of AML Leukemic Stem Cells
Article Snippet: After again two washing steps, 100 μL/well goat anti-rabbit IgG (for detection of DARPins; ThermoFisher Scientific), goat-anti human IgG (for detection of CD123-CD3 ADAPTIR; ThermoFisher Scientific), or mouse anti-Penta His (for detection of CD33-CD3 BiTE and CD123-CD3 DART; Qiagen) antibodies (1:1,000 diluted in FACS buffer) were added to wells. .. After subsequent incubation at 4°C for 20 minutes, cells were again washed twice with FACS buffer and finally fixed with BD Biosciences Fixation Buffer (1:10 diluted in water). ..



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