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Macrogen big dye terminator cycle sequencing kit
Big Dye Terminator Cycle Sequencing Kit, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/big+dye+terminators/bigdye+cycle+kit+sequencing+terminator/pm41850155-54-11-5
Average 86 stars, based on 1 article reviews
big dye terminator cycle sequencing kit - by Bioz Stars, 2026-11
86/100 stars

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Polymerase Chain Reaction:

Article Title: Roselle (Hibiscus sabdariffa) stem residues as a sustainable plant-based culture medium for isolation of endophytic bacteria.
Article Snippet: .. The PCR conditions consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 50 °C for 30 s, and extension at 72 °C for 1.5 min, with a final extension at 72 °C for 10 min. Scientific Reports | (2026) 16:14394 4| https://doi.org/10.1038/s41598-026-47923-7 PCR products were purified and sequenced in both forward and reverse directions using the BigDye® Terminator v3.1 Cycle Sequencing Kit on an ABI PRISM 3730XL Analyzer (Macrogen Inc., Korea). ..

Article Title: Mycological Survey of Fungal Carriage in Cats and Their Owners: Analysis of Species Diversity and Antifungal Susceptibility.
Article Snippet: .. All PCR products were purified and sequenced in both the forward and reverse directions using ABI BigDye Terminator V.3.1 (Macrogen Inc., Seoul, Korea). .. Sequences were assembled and manually edited for consistency using BioEdit v7.2.5 software (https://bioedit.software.informal.com).

Article Title: Hippobosca equina: Influences on oxidative stress and immune-regulating cytokine responses in horses.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Amplicons were purified using a QIAquick PCR purification kit (Qiagen, USA) and sequenced bidirectionally by Macrogen Inc. (Seoul, South Korea) using the BigDye Terminator Cycle Sequencing Kit and a 3730XL sequencer (Applied BiosystemsTM, USA). .. Sequences were edited and assembled using BioEdit software and submitted to GenBank, resulting in accession numbers PQ637294.1 and PQ637295.1 for the newly sequenced variants.

Article Title: Molecular epidemiology and antifungal susceptibility patterns of Aspergillus and Candida species isolated from Argentinian patients with COVID-19-associated pulmonary aspergillosis and candidemia.
Article Snippet: .. PCR products were puried using the PCR and DNA fragment purification kitTM Dongsheng Biotech, Guangzhou, Guangdong, China) and idirectionally sequenced using the ABI BigDye Terminator .3.1 kit (Macrogen Inc., Seoul, South Korea). ..

Purification:

Article Title: Roselle (Hibiscus sabdariffa) stem residues as a sustainable plant-based culture medium for isolation of endophytic bacteria.
Article Snippet: .. The PCR conditions consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 50 °C for 30 s, and extension at 72 °C for 1.5 min, with a final extension at 72 °C for 10 min. Scientific Reports | (2026) 16:14394 4| https://doi.org/10.1038/s41598-026-47923-7 PCR products were purified and sequenced in both forward and reverse directions using the BigDye® Terminator v3.1 Cycle Sequencing Kit on an ABI PRISM 3730XL Analyzer (Macrogen Inc., Korea). ..

Article Title: Mycological Survey of Fungal Carriage in Cats and Their Owners: Analysis of Species Diversity and Antifungal Susceptibility.
Article Snippet: .. All PCR products were purified and sequenced in both the forward and reverse directions using ABI BigDye Terminator V.3.1 (Macrogen Inc., Seoul, Korea). .. Sequences were assembled and manually edited for consistency using BioEdit v7.2.5 software (https://bioedit.software.informal.com).

Article Title: Hippobosca equina: Influences on oxidative stress and immune-regulating cytokine responses in horses.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Amplicons were purified using a QIAquick PCR purification kit (Qiagen, USA) and sequenced bidirectionally by Macrogen Inc. (Seoul, South Korea) using the BigDye Terminator Cycle Sequencing Kit and a 3730XL sequencer (Applied BiosystemsTM, USA). .. Sequences were edited and assembled using BioEdit software and submitted to GenBank, resulting in accession numbers PQ637294.1 and PQ637295.1 for the newly sequenced variants.

Article Title: First report of the giant kidney worm Dioctophyme renale in a domestic dog from Costa Rica.
Article Snippet: Dioctophyme renale, the giant kidney worm, is a parasitic nematode of domestic and wild carnivores, occasionally infecting humans.. While widely reported in South America, it has not been documented in Central America before.. A 3-year-old French bulldog presented with leukocytosis, thrombocytopenia, azotemia, pyrexia, vomiting, and apathy.

Article Title: Molecular epidemiology and antifungal susceptibility patterns of Aspergillus and Candida species isolated from Argentinian patients with COVID-19-associated pulmonary aspergillosis and candidemia.
Article Snippet: .. PCR products were puried using the PCR and DNA fragment purification kitTM Dongsheng Biotech, Guangzhou, Guangdong, China) and idirectionally sequenced using the ABI BigDye Terminator .3.1 kit (Macrogen Inc., Seoul, South Korea). ..

Sequencing:

Article Title: Roselle (Hibiscus sabdariffa) stem residues as a sustainable plant-based culture medium for isolation of endophytic bacteria.
Article Snippet: .. The PCR conditions consisted of an initial denaturation at 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 50 °C for 30 s, and extension at 72 °C for 1.5 min, with a final extension at 72 °C for 10 min. Scientific Reports | (2026) 16:14394 4| https://doi.org/10.1038/s41598-026-47923-7 PCR products were purified and sequenced in both forward and reverse directions using the BigDye® Terminator v3.1 Cycle Sequencing Kit on an ABI PRISM 3730XL Analyzer (Macrogen Inc., Korea). ..

Article Title: Hippobosca equina: Influences on oxidative stress and immune-regulating cytokine responses in horses.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Amplicons were purified using a QIAquick PCR purification kit (Qiagen, USA) and sequenced bidirectionally by Macrogen Inc. (Seoul, South Korea) using the BigDye Terminator Cycle Sequencing Kit and a 3730XL sequencer (Applied BiosystemsTM, USA). .. Sequences were edited and assembled using BioEdit software and submitted to GenBank, resulting in accession numbers PQ637294.1 and PQ637295.1 for the newly sequenced variants.

Article Title: Genetic diversity and phylogeography of Hyalomma scupense, the main vector of tropical theileriosis: Global analysis with novel data from Tunisia.
Article Snippet: Hyalomma scupense, the main vector of tropical theileriosis, presents taxonomic challenges that impede vector surveillance.. This study combines targeted sampling of 20 morphologically-confirmed specimens from El Hessiene, Tunisia (3650'N 1012'E) with global GenBank analysis of mitochondrial markers 16S rRNA, 12S rRNA, and COI to assess genetic diversity, phylogeographic structure, and regional gene flow.. All Tunisian sequences showed 99.88–100% identity to H. scupense references.

Article Title: Heat-Assisted Extraction and Bioactivity Evaluation of a Dinactin-Associated Compound from Streptomyces UP Strains
Article Snippet: .. The sequencing was performed using an automated Applied Biosystems 3100 sequencer with the BigDye® Terminator v3.1 Cycle Sequencing Kit (Macrogen Inc., Seoul, Republic of Korea). .. The nucleotide sequence data were validated and edited using BioEdit software (version 7.2; Informer Technologies Inc., Los Angeles, CA, USA).

Article Title: Identification of OPN1LW Exon 3 Variants Impairing Red-Cone Function in Color Vision Deficiency
Article Snippet: .. Macrogen performed all subsequent services, including the cleanup of the sequencing reaction and the actual Sanger sequencing. .. Unidirectional sequencing of exon 3 was performed on an ABI 3730xl sequencer (Applied Biosystems) using the BigDye Terminator v3.1 chemistry and 10 pmol of the reverse primer (5′-CCACTCCATCTTGCGTCCTC-3′).

Article Title: First report of the giant kidney worm Dioctophyme renale in a domestic dog from Costa Rica.
Article Snippet: Dioctophyme renale, the giant kidney worm, is a parasitic nematode of domestic and wild carnivores, occasionally infecting humans.. While widely reported in South America, it has not been documented in Central America before.. A 3-year-old French bulldog presented with leukocytosis, thrombocytopenia, azotemia, pyrexia, vomiting, and apathy.

DNA Sequencing:

Article Title: Identification of OPN1LW Exon 3 Variants Impairing Red-Cone Function in Color Vision Deficiency
Article Snippet: .. Macrogen performed all subsequent services, including the cleanup of the sequencing reaction and the actual Sanger sequencing. .. Unidirectional sequencing of exon 3 was performed on an ABI 3730xl sequencer (Applied Biosystems) using the BigDye Terminator v3.1 chemistry and 10 pmol of the reverse primer (5′-CCACTCCATCTTGCGTCCTC-3′).

Staining:

Article Title: First report of the giant kidney worm Dioctophyme renale in a domestic dog from Costa Rica.
Article Snippet: Dioctophyme renale, the giant kidney worm, is a parasitic nematode of domestic and wild carnivores, occasionally infecting humans.. While widely reported in South America, it has not been documented in Central America before.. A 3-year-old French bulldog presented with leukocytosis, thrombocytopenia, azotemia, pyrexia, vomiting, and apathy.



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