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big dye terminator v3.1 cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher big dye terminator v3.1 cycle sequencing kit
    Big Dye Terminator V3.1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/big+dye+terminators/bigdye+terminator+v3+1+cycle+sequencing+kit/pm40640756-66-26-33
    Average 90 stars, based on 1 article reviews
    big dye terminator v3.1 cycle sequencing kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: The identification of novel missense variant in ChAT gene in a patient with gestational diabetes denotes plausible genetic association
    Article Snippet: The amplified PCR products were cleaned up, and the sequencing reaction was performed with a BigDye ® Terminator kit (Applied Biosystems), and capillary electrophoresis was performed using the ABI PRISM ® 3130xl Genetic Analyser (Applied Biosystems by ThermoFisher Scientific).

    Article Title: Targeting of bowel tissue by fully human antibodies cross-reactive with human and mouse GPA33 antigen.
    Article Snippet: Sequence determination of these purified nucleotides was carried out with a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, 4337455).

    Article Title: Fatal babesiosis in a free-ranging iberian wolf co-infected with Hepatozoon canis and Leishmania infantum : Epidemiological implications for the cantabrian wolf population
    Article Snippet: Negative controls for extraction, negative PCR controls (water instead of DNA) and positive controls containing DNA of Babesia bigemina or Theileria orientalis complex [Zoonosis collection, registered in the National Registry of Biobanks from Carlos III Health Institute (Reference: C.0006409) and located at CRETAV] were included in the PCR assays. qPCR and PCR amplicons were sequenced using the BigDye ® Terminator v3·1 Cycle Sequencing Kit (Thermo Fisher Scientific), following the manufacturer's instructions, on an ABI PRISM and a SeqStudio genetic analysers (Thermo Fisher Scientific), respectively.

    Article Title: Development of a Multiplex Real-Time PCR Assay for the Rapid Detection of the Asian-Type DEL.
    Article Snippet: Sequencing was performed with the BigDye Terminator version 3.1 cycle sequencing kit (Thermo Fisher Scientific) and the 3500 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Rod-shaped microglia represent a morphologically distinct subpopulation of disease-associated microglia.
    Article Snippet: Sanger sequencing was performed using the forward primer and the PCR product with the BigDye Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific).

    Sequencing:

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA.
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. 2023), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..

    Article Title: When Two Species Meet: A Potential Beetle-Yeast Facultative Mutualism.
    Article Snippet: .. We conducted PCR reactions as follows: LSU—96°C for 3 min, 35 cycles at 96°C for 30 s, 61°C for 45 s and 72°C for 1 min; ITS—96°C for 3 min, 35 cycles at 94°C for 1 min, 55°C for 1 min, and 72°C for 2 min. We purified the amplicons with Wizard SV Gel and PCR Clean- Up System (Promega, Madison, WI, USA) and sequenced using BigDye Terminator v. 3.1 Cycle Sequencing Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA), following the manufacturer's protocols. ..

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. ), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..

    Polymerase Chain Reaction:

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA.
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. 2023), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..

    Article Title: When Two Species Meet: A Potential Beetle-Yeast Facultative Mutualism.
    Article Snippet: .. We conducted PCR reactions as follows: LSU—96°C for 3 min, 35 cycles at 96°C for 30 s, 61°C for 45 s and 72°C for 1 min; ITS—96°C for 3 min, 35 cycles at 94°C for 1 min, 55°C for 1 min, and 72°C for 2 min. We purified the amplicons with Wizard SV Gel and PCR Clean- Up System (Promega, Madison, WI, USA) and sequenced using BigDye Terminator v. 3.1 Cycle Sequencing Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA), following the manufacturer's protocols. ..

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. ), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..

    Purification:

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA.
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. 2023), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..

    Article Title: When Two Species Meet: A Potential Beetle-Yeast Facultative Mutualism.
    Article Snippet: .. We conducted PCR reactions as follows: LSU—96°C for 3 min, 35 cycles at 96°C for 30 s, 61°C for 45 s and 72°C for 1 min; ITS—96°C for 3 min, 35 cycles at 94°C for 1 min, 55°C for 1 min, and 72°C for 2 min. We purified the amplicons with Wizard SV Gel and PCR Clean- Up System (Promega, Madison, WI, USA) and sequenced using BigDye Terminator v. 3.1 Cycle Sequencing Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA), following the manufacturer's protocols. ..

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. ), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..

    Amplification:

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA.
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. 2023), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..

    Article Title: Ewingella allii sp. nov. isolated from a diseased onion plant in the Columbia Basin of Washington State, USA
    Article Snippet: .. The sequencing PCR reaction mixture was done by mixing 1 μl of the purified PCR amplicon (Mushtaq et al. ), 5 μl of 5X sequencing buffer, 0.5 μl of BigDye Terminator (Thermo Scientific, Pennsylvania, USA) 0.5 μl of 25 nmoles forward primer, and 6.5 μl of nuclease-free water. ..



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