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test kits for β-amylase activity β-amy  (Beijing Solarbio Science)


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    Beijing Solarbio Science test kits for β-amylase activity β-amy
    Test Kits For β Amylase Activity β Amy, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/beta+test+kit/%CE%B1+amylase+assay+kit/pmc12279718-75-21-25
    Average 90 stars, based on 1 article reviews
    test kits for β-amylase activity β-amy - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Proline Improves Pullulan Biosynthesis Under High Sugar Stress Condition
    Article Snippet: The activities of α-amylase (AMY) and isopullulanase (IPU) were determined using the α-Amylase Activity Assay Kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) and the Isopullulanase Activity Assay Kit (Suzhou Grace Biotechnology Co., Ltd., Suzhou, China), respectively.

    Article Title: Polysaccharide from Gynostemma pentaphyllum Makino Modulates the Oxidative Stress Response under an Acute Hypobaric Hypoxia Environment by Enhancing the Relative Abundance of Anti-inflammatory Gut Microbiota.
    Article Snippet: Gynostemma pentaphylla Makino, a plant renowned for its dual medicinal and culinary properties, is rich in highquality phytochemicals with significant therapeutic potential.. Polysaccharides represent one of their key bioactive components.. This study investigated the mechanism of polysaccharide from Gynostemma pentaphyllumMakino (GPMP) in counteracting high-altitude hypoxic stress.

    Article Title: Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress
    Article Snippet: The soluble sugar metabolism index was assessed using test kits for starch (Sta), glucose (Glu), sucrose (Suc), α-amylase activity (α-AMY), and β-amylase activity (β-AMY) from Solarbio, China, as well as maltose (Mal) from OKA, China.

    Article Title: Screening microorganisms with robust and stable protein expression and secretion capacity.
    Article Snippet: Review Editor: Aitziber L. Cortajarena Abstract Robust and stable protein secretion is crucial for efficient recombinant protein production.. Here, a novel and powerful platform using split GFP activated droplet sorting (SGADS) has been developed to significantly boost the yields of the protein of interest (POI).. The SGADS platform leverages solubilizing peptide P17 and secretory expression in Bacillus subtilis to optimize two split GFP sensors: the P17-GFP1-9/GFP10-POI-GFP11 sensor for assessing protease activity and the P17-GFP1-10/GFP11-POI sensor for measuring secretion capacity.

    Article Title: The regulation of cassava nanostarch structure treated with malt endogenous enzymes and adsorption property for anthocyanins.
    Article Snippet: The enzymatic preparation of nanostarch exhibits specific and efficient.. However, commercial amylases face issues of expensive and time-consuming extraction, which limit their application.. This study obtained cassava nanostarch (MCS) from Cassava starch (CS) treated with malt endogenous amylase, followed by structural characterization and property exploration.

    Article Title: Influence of serotonin (5-HT) on locomotor behavior and digestive physiology in the sea cucumber Apostichopus japonicus
    Article Snippet: The sea cucumber Apostichopus japonicus exhibits distinct physiological processes such as aestivation, seasonal reproduction, and regeneration, all of which are significantly regulated by neurohormones.. Serotonin (5-hydroxytryptamine, 5-HT) is known to regulate behavior in various species, particularly influencing feeding and locomotion.. However, its effects on the behavior of sea cucumbers remain largely unexamined.

    Activity Assay:

    Article Title: Expandable konjac fiber modulates appetite and chyme digestion in vivo by stomach-intestine-brain axis.
    Article Snippet: Appetite regulation is a lifestyle intervention strategy to maintain health.. The regulatory effects of dietary fiber (especially insoluble dietary fiber), as a crucial element of the nutritional composition, on appetite remain poorly understood.. This study investigated modulatory effects of konjac fiber (KF, with high and low expansion) and konjac powder (KP) on chyme digestion, gastrointestinal hormones, intestinal microbiota, appetite genes in hypothalamus, GLP-1 receptor (GLP-1R) protein in various tissues of rats by dietary intervention.



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    TAF15 facilitates M2-like polarization of macrophages, enhancing <t>TGFBI</t> secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) <t>ELISA</t> analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.
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    TAF15 facilitates M2-like polarization of macrophages, enhancing <t>TGFBI</t> secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) <t>ELISA</t> analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.
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    TAF15 facilitates M2-like polarization of macrophages, enhancing <t>TGFBI</t> secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) <t>ELISA</t> analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.
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    TAF15 facilitates M2-like polarization of macrophages, enhancing <t>TGFBI</t> secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) <t>ELISA</t> analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.
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    TAF15 facilitates M2-like polarization of macrophages, enhancing <t>TGFBI</t> secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) <t>ELISA</t> analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.
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    TAF15 facilitates M2-like polarization of macrophages, enhancing <t>TGFBI</t> secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) <t>ELISA</t> analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.
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    Image Search Results


    TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

    Journal: JHEP Reports

    Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

    doi: 10.1016/j.jhepr.2025.101545

    Figure Lengend Snippet: TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

    Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

    Techniques: Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay

    M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

    Journal: JHEP Reports

    Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

    doi: 10.1016/j.jhepr.2025.101545

    Figure Lengend Snippet: M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

    Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

    Techniques: In Vivo, In Vitro, Imaging, Immunofluorescence, Western Blot, Transfection, Encapsulation, Liposomes, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Derivative Assay, Immunohistochemistry, Real-time Polymerase Chain Reaction, Binding Assay