Journal: Molecular Neurobiology

Article Title: Silibinin Alleviates Cognitive Dysfunction, Biochemical Alterations, and Hippocampal Structural Changes Induced by 5 days of Sleep Deprivation with or Without a Subsequent 24-H Recovery in Rats

doi: 10.1007/s12035-026-06195-y

Figure Lengend Snippet: Effect of silibinin administration on A BDNF (ng/ml) and B CaMKII (ng/mg) in sleep-deprived rats with and without consequent 24-h recovery. Data are represented as mean ± SD ( n = 8); group comparisons were performed using one-way ANOVA, followed by Tukey’s multiple-comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus the control group; $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 versus the SD group; # P < 0.05, ## P < 0.01, and ### P < 0.001 versus the SD + R group. SD: sleep-deprived untreated group; SD + R: sleep-deprived untreated group with recovery; SD + Sil: sleep-deprived group treated with silibinin; SD + Sil + R: sleep-deprived group treated with silibinin followed by recovery. BDNF: brain‑derived neurotrophic factor; CaMKII: calcium/calmodulin‑dependent protein kinase II

Article Snippet: Furthermore, hippocampal tissue homogenates were prepared to determine BDNF (Boster Biological Technology, Pleasanton, CA, USA; Cat. No. EK0308) and CaMKII (BlueGene Biotech, Shanghai, China; Cat. No. E02C0682) using commercially available ELISA kits supplied by Bio-KITS (Cairo, Egypt), according to the manufacturers' instructions.

Techniques: Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

doi: 10.3389/fcell.2026.1929409

Figure Lengend Snippet: Primer sequences for RT-qPCR analysis.

Article Snippet: Following transfer, membranes were blocked and incubated overnight at 4 °C with the indicated primary antibodies: GPR81 (1:400, Sigma, SAB1300790), p-ERK1/2 (1:1000, CST, 4370), ERK1/2 (1:1000, CST, 4695), BDNF (1:1000, Proteintech, 28205-1-AP), SYN (1:1000, CST, 4329), PSD95 (1:1000, CST, 3450), PGC-1α (1:1000, Novus, NBP1-04676SS), NRF1 (1:1000, CST, 46743), NRF2 (1:1000, CST, 12721), TFAM (1:2000, Abcam, ab131607), OPA1 (1:1000, CST, 8047), MFN1 (1:2000, Proteintech, 13798-1-AP), MFN2 (1:1000, CST, 9482), and β-Tubulin (1:2000, Proteintech, 10068-1-AP).

Techniques: Analysis, Sequencing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

doi: 10.3389/fcell.2026.1929409

Figure Lengend Snippet: Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and PSD95, derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.

Article Snippet: Following transfer, membranes were blocked and incubated overnight at 4 °C with the indicated primary antibodies: GPR81 (1:400, Sigma, SAB1300790), p-ERK1/2 (1:1000, CST, 4370), ERK1/2 (1:1000, CST, 4695), BDNF (1:1000, Proteintech, 28205-1-AP), SYN (1:1000, CST, 4329), PSD95 (1:1000, CST, 3450), PGC-1α (1:1000, Novus, NBP1-04676SS), NRF1 (1:1000, CST, 46743), NRF2 (1:1000, CST, 12721), TFAM (1:2000, Abcam, ab131607), OPA1 (1:1000, CST, 8047), MFN1 (1:2000, Proteintech, 13798-1-AP), MFN2 (1:1000, CST, 9482), and β-Tubulin (1:2000, Proteintech, 10068-1-AP).

Techniques: Expressing, Western Blot, Analysis, Derivative Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

doi: 10.3389/fcell.2026.1929409

Figure Lengend Snippet: Effects of mitochondrial dysfunction on expression of genes and proteins related to synaptic plasticity in Neuro-2A cells. (a-e) Quantitative analysis (qRT-PCR) of Arc , Fos , Egr1 , bdnf , and Syp , derived from three independent replicates. (f) Exemplary Western blot image. (g-i) Bar graphs depict the quantified protein levels for BDNF, SYN, and PSD95, derived from six independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the LA group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 3OBA group. n. s., no significant difference.

Article Snippet: Following transfer, membranes were blocked and incubated overnight at 4 °C with the indicated primary antibodies: GPR81 (1:400, Sigma, SAB1300790), p-ERK1/2 (1:1000, CST, 4370), ERK1/2 (1:1000, CST, 4695), BDNF (1:1000, Proteintech, 28205-1-AP), SYN (1:1000, CST, 4329), PSD95 (1:1000, CST, 3450), PGC-1α (1:1000, Novus, NBP1-04676SS), NRF1 (1:1000, CST, 46743), NRF2 (1:1000, CST, 12721), TFAM (1:2000, Abcam, ab131607), OPA1 (1:1000, CST, 8047), MFN1 (1:2000, Proteintech, 13798-1-AP), MFN2 (1:1000, CST, 9482), and β-Tubulin (1:2000, Proteintech, 10068-1-AP).

Techniques: Expressing, Analysis, Quantitative RT-PCR, Derivative Assay, Western Blot

Journal: Drug Design, Development and Therapy

Article Title: Everolimus Attenuates Salicylate-Induced Tinnitus-Like Behavior and Auditory Cortical Hyperexcitability

doi: 10.2147/DDDT.S632911

Figure Lengend Snippet: Everolimus mitigates the aberrant upregulation of BDNF in the primary auditory cortex of tinnitus rats.

Article Snippet: Subsequently, they were incubated overnight at 4°C with primary antibodies against phospho-mTOR (Ser2448) (ABclonal, Cat. AP1413), mTOR (ABclonal, Cat. A2445), BDNF (ABclonal, Cat. A4873), PSD95 (Abcam, Cat. ab18258), and Vinculin (ServiceBio, Cat. GB150068 ) as a loading control.

Techniques: